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Citations for Vector Labs :
1451 - 1500 of 1511 citations for GATOR2 complex protein WDR24 WDR24 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... the slides were incubated with a biotin-conjugated secondary antibody for 1 h and with VECTASTAIN ABC Reagent (Cat. #PK- 4000; Vector Laboratories, Burlingame, CA, USA) for 30 min at 20°C ...
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bioRxiv - Immunology 2022Quote: ... were incubated at 4°C overnight followed by washing and incubation with a secondary biotinylated goat anti-mouse IgG antibody (dilution: 1:200, Vector Laboratories, Burlingame, California, USA). For color development freshly prepared avidin-biotin-peroxidase complex (ABC ...
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bioRxiv - Immunology 2020Quote: ... and then washed three times in PBST before incubation with biotinylated-secondary antibodies dissolved in PBST (BA-1000 for anti-rabbit and BA-9200 for anti-mouse; Vector Laboratories, 1:1,000 dilution) at 25°C for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... incubated in the appropriate biotinylated secondary antibody (goat anti-rabbit, #BA-100; goat anti-mouse, Vector Labs #BA-9200;Vector Laboratories, Burlington, ON Canada) and then incubated with avidin-biotin complex (Vectastain ABC kit ...
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bioRxiv - Neuroscience 2023Quote: ... The blots were washed with 1X TBST thrice for 5 minutes and then incubated with HRP-conjugated secondary antibodies (1:4000, Vector Labs, Burlingame, CA, USA) for 1-2 hours at RT ...
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bioRxiv - Neuroscience 2023Quote: For light and electron microscopic analysis when we visualized the L5 fibers in the brainstem after the anti-mCherry primary antibody we used anti-rabbit ImmPRESS (1:2, Vector Laboratories Burlingame, Ca 94010) and DAB-Ni/DAB (DAB-Ni ...
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bioRxiv - Neuroscience 2023Quote: ... incubated for 1 hour at room temperature in biotinylated anti-mouse secondary antibody at 1:500 dilution (Vector Laboratories; cat # BA-9200; RRID: AB_2336171), followed by incubation in ABC Media 1:800 (Vector Laboratories ...
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bioRxiv - Pathology 2023Quote: ... Slides were then washed with PBS and incubated at room temperature for one hour with appropriate secondary antibodies (Vector Laboratories MP- 7401, MP-7402). Slides were developed with diaminobenzidine substrate (Vector Laboratories SK-4100 ...
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bioRxiv - Pathology 2023Quote: ... the sections were incubated for 30 min with a secondary antibody (peroxidase-labeled anti-mouse/anti-rabbit IgG (ImmPRESS kit, Vector Labs, Burlingame, CA, USA)) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cleaved caspase 8 immunohistochemistry staining was performed using cleaved caspase 8 primary antibody (1:100; Cell Signalling Technology, cat. #9496) and goat anti-rabbit biotinylated secondary (1:200; Vector Laboratories, cat. BA-1000). Briefly ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Peroxidase-conjugated avidin and the secondary biotinylated antibodies rabbit anti-mouse IgG and rabbit fblanti-goat used in immunohistochemical studies were from VECTASTAIN® Elite ABC-Kit (Vector Laboratories, Burlingame, CA)
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bioRxiv - Neuroscience 2023Quote: ... This was followed by incubation with biotinylated horse anti-mouse IgG secondary antibody (1:200, PK-6102, Vectastain Elite ABC HRP kit, Vector Laboratories, USA, RRID: AB_2336821) for 30 min at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were probed with fluorochrome-conjugated secondary antibodies for 1 hour at room temperature and mounted using VECTASHIELD Antifade Mounting Medium with DAPI (Vector Laboratories, H-1200-10). Images were taken with the LSM 880 confocal microscope (Zeiss).
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bioRxiv - Cancer Biology 2024Quote: ... and then stained with secondary anti-mouse IgG and anti-rabbit IgG HRP-conjugated antibodies for 30 min at RT (Vector Laboratories, MP-7500-50). After washing twice in washing buffer for 5 min each ...
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bioRxiv - Microbiology 2023Quote: ... Parasites were then stained with an anti-VSG-2 monoclonal antibody (Hovel-Miner et al., 2012) conjugated to Alexa647 and mounted in Vectashield (Vector Laboratories, H-1800-10) containing 4′,6-diamidino-2-phenylindole (DAPI) ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated at room temperature for 1.5 hours in secondary antibody solution containing either biotinylated rabbit anti-goat IgG (Vector Laboratories, Burlingame, CA; PK-6105) or biotinylated goat anti-rabbit IgG (Vector Laboratories ...
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bioRxiv - Developmental Biology 2022Quote: ... immunofluorescence was performed on paraffin sections using antibodies in Supplemental Table 1b and the following reagents: ImmPRESS(r) HRP Horse Anti-Rabbit IgG Polymer Detection Kit (Vector Labs, MP-7401-50), ImmPRESS(r ...
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bioRxiv - Neuroscience 2023Quote: ... Detection of antibody signal was revealed with 3,3’-diaminobenzidine (DAB) peroxidase substrate (DAB Substrate Kite, Peroxidase with Nickel, Vector Labs #SK-4100, Burlingame, CA).
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bioRxiv - Cell Biology 2023Quote: ... HRP-conjugated secondary antibody followed by 3,3’-Diaminobenzidine (DAB) staining using the ImmPRESS™ Excel Amplified HRP peroxidase Polymer Staining Kit (Vector Laboratories MP-7602-15), according to the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and incubated for 2-3 hours with biotinylated anti-rabbit secondary antibody (1:450 for DeltaFosB and 1:600 for c-Fos, Vector Laboratories, Burlingame, CA, USA). Finally ...
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Tumor microenvironment acidosis favors pancreatic cancer stem cell properties and in vivo metastasisbioRxiv - Cancer Biology 2024Quote: ... The following day, the sections were incubated with secondary antibodies (1 h, room temperature) and mounted with Vectashield® Mounting Medium (Vector Laboratories, #H-1000). Image acquisition was performed using a Leica SP8 confocal microscope ...
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bioRxiv - Neuroscience 2024Quote: ... Slides were washed in PBS two times for 10 minutes and then primary antibodies were applied overnight in 10% normal horse serum (Vector Labs, #S-2000-20), 0.5% Triton-X-10 (Sigma ...
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bioRxiv - Bioengineering 2024Quote: ... Membranes were washed with TBS-T for 5 minutes three times on a shaker at room temperature and incubated with HRP-conjugated secondary antibody (1:8000 in TBS-T; Vector Laboratories, PI-2000-1) for one hour at RT ...
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bioRxiv - Neuroscience 2024Quote: ... a blocking serum is applied from the same species as the one used to produce the secondary antibody (ImmPRESS™ Anti-Rabbit and Anti-Mouse Kit, Vector Labs, Burlington, USA). The blocking process was carried out at room temperature for at least 20 minutes.
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bioRxiv - Cell Biology 2024Quote: ... hydrogen peroxide in methanol to block endogenous peroxidase and then incubated with Normal Goat Serum Blocking Solution for rabbit antibody (S-1000, Vector Laboratories, Newark, CA, USA) to block nonspecific sites ...
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bioRxiv - Neuroscience 2024Quote: ... for 30 min, a first antibody solution (PB 0.1M, Triton 0.3%, NGS 2%, Rabbit α-Phal-L 1:8000 (Vector Laboratories, AS-2300, RRID: AB_2313686), Mouse α-PV 1:4000 (Swant ...
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bioRxiv - Neuroscience 2024Quote: ... followed by incubation with an appropriate biotinylated secondary antibody (goat anti-rabbit, horse anti-goat, or horse anti-mouse; Vector Laboratories, Burlingame, CA, USA) diluted 1:200 in T-KPBS for two hours at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Slides were incubated with the primary antibody overnight at 4 °C and processed using the VECTASTAIN® Elite ABC-HRP Rabbit IgG Kit (Vector Laboratories, #PK-6101) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed 3 times with TBS-Tx to remove unbound primary antibody then prepared using the Rabbit IgG VECTASTAIN® Elite ABC-HRP Kit (PK-6101, Vector Laboratories, Burlingame, CA). Briefly ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by incubation in secondary antibody (biotinylated goat anti-rabbit, 1:200, or biotinylated goat anti-mouse, 1:200, Vector Laboratories, Burlingame, CA, USA) for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... tissues were incubated with primary and secondary antibodies as above and then stained with DAPI and mounted with Vectashield antifade mounting medium (Vector Labs, Newark, CA, USA). Images were captured using a Leica TCS SP5 confocal microscope ...
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bioRxiv - Cancer Biology 2024Quote: ... Cryosections were blocked in PBS containing 10% donkey serum and 0.1% Triton X-100 for 2 h and incubated overnight at 4°C with rabbit anti-Iba-1 and goat anti-Arg1 antibodies or FITC-conjugated Lycopersicon esculentum (Tomato) Lectin (Vector Labs, FL-1171-1). Next ...
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bioRxiv - Microbiology 2021Quote: ... was used to stain nucleic acids following washing away unbound secondary antibodies and samples were covered with VectaShield anti-fade reagent (Vector Laboratories, VWR, Cat# 101098-048) and a coverslip ...
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bioRxiv - Cancer Biology 2020Quote: ... Primary antibodies were incubated with sections overnight at 4°C and detected using Vectastain Elite ABC HRP Kits (Vector Laboratories: Peroxidase Rabbit IgG PK-6101) and DAB substrate (Vector Laboratories ...
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bioRxiv - Microbiology 2022Quote: ... the secondary biotinylated goat anti-mouse or anti-rabbit antibody was applied for 30 minutes at room temperature (Vector Laboratories, Burlingame, CA, USA, 1:200). Slides were scanned using Hamamatsu S60 scanner ...
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bioRxiv - Developmental Biology 2024Quote: ... immunofluorescence was performed on paraffin sections using antibodies in Supplementary Table 2 and the following reagents: ImmPRESS® HRP Horse Anti-Rabbit IgG Polymer Detection Kit (Vector Labs, MP-7401-50). Following the application of TSA fluorophores (listed in Supplementary Table 2 ...
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bioRxiv - Microbiology 2022Quote: ... Slides were washed thrice with PBS to remove excess antibody and incubated with a rabbit anti-goat biotinylated IgG (Vector laboratories catalogue number BA-5000) for 10 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were washed with PBS (3 x 10 min) and incubated with biotinylated goat anti-rabbit secondary antibody (1:600, Vector Laboratories, Cat# BA-1000, RRID:AB_2313606), diluted in 1% NGS in PBS with Triton X-100 ...
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bioRxiv - Cell Biology 2024Quote: Secondary antibodies were Horse Radish Peroxidase conjugated goat anti-rabbit or anti-mouse (ImmPRESS Polymer Detection Kits, Peroxidase, Vector Laboratories, MP-7451 and MP-7452) or Dako REAL EnVision detection system (Dako ...
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bioRxiv - Molecular Biology 2024Quote: ... Optimization of all antibodies was performed on native postmenopausal ovarian tissue containing both ovarian cortex and medulla along with positive controls (High Grade Serous Ovarian Carcinoma tissue sections) and non-immune controls (Rabbit and Mouse IgG control antibodies, Vector Laboratories Inc., Burlingame, CA, USA) at the same concentrations as the corresponding primary antibody ...
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bioRxiv - Pathology 2024Quote: ... the primary antibody was detected using the ImmPRESS® HRP Horse Anti-Rabbit IgG PLUS Polymer Kit (Vector Laboratories, MP-7801-15, Newark, California, USA) following manufacturer instructions.
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bioRxiv - Pathology 2024Quote: ... slides were incubated with secondary antibody solution with DAPI for 1 hr at RT and mounted using VECTASHIELD® Antifade Mounting Medium (Vector Laboratories, H-1000-10). Stained tissues were then imaged under EVOS Cell Imaging System (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... slides were incubated in secondary antibody for 1hr at room temperature: goat anti-Rabbit (ImmPRESS HRP Goat Anti-Rabbit IgG Polymer Detection Kit, Vector Laboratories, Cat. No.: MP-7451). Immunohistochemistry slides were developed using ImmPact DAB Brown (Vector Labs ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were washed three times with PBS-T and incubated for 1 hr at room temperature with the corresponding horseradish peroxidase (HRP)-labelled Secondary antibodies: Goat Anti-Rabbit IgG (H+L) (Vector Laboratories, (BA-1000; 1:2000) and Rabbit Anti-Rat IgG (H+L ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated with a rabbit polyclonal anti-syncollin antibody for 2 h at room temperature followed by an FITC-conjugated goat anti-rabbit secondary antibody and 4′,6-diamidino-2-phenylindole (DAPI, Vector Laboratories, Burlingame, CA; 1.5 μg.mL−1).
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bioRxiv - Neuroscience 2021Quote: ... sections were washed in PBST and incubated for 60 minutes at room temperature with a secondary biotinylated goat anti-rabbit antibody (Vector laboratories, Cat# BA-1000, lot# 2F0430) diluted 1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... the samples were washed twice for 20 min in 2XSSC at 42°C and subsequently incubated with fluorescein anti-biotin antibody (Vector Laboratories, SP-3040; Dilution 1:100) for at least 45min ...
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bioRxiv - Molecular Biology 2023Quote: ... slides were incubated for 1 hour with a secondary antibody cocktail: 1:250 anti-rabbit IgG Texas Red 594 (Vector Labs, Newark, CA, USA; TI-1000) + anti-mouse IgG2b AF488 (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... The 3,3′-diaminobenzidine (DAB) based-chromogenic detection was performed using biotinylated secondary antibodies in conjunction with VECTASTAIN® ELITE® ABC HRP Detection Kit (Vector Laboratories, Cat# PK-6100) and ImmPACT® DAB Substrate Kit ...
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bioRxiv - Cell Biology 2024Quote: ... followed by an ALP-coupled goat anti-mouse immunoglobulin G secondary antibody (ImmunoLogic, WellMed, MS Arnhem, Netherlands) and ImmPACT® Vector® Red Substrate (Vector Laboratories, Newark, CA, USA) visualization.