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Citations for Vector Labs :
1401 - 1449 of 1449 citations for Mouse Anti Human Ig kappa chain Alexa Fluor 647 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... or the corresponding secondary antibodies: biotinylated goat anti-guinea pig antibody (1:100 dilution for GFP detection; Vector Laboratories, Cat# PK-4007; RRID:AB_2336816) and secondary antibody goat anti-mouse IgG coupled to 1.4 nm gold (1:100 dilution for TH detection ...
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bioRxiv - Neuroscience 2023Quote: ... USA) followed by secondary biotinylated antibody incubation (1:200, goat anti-rabbit IgG, Cat. No. BA-1000; Vector Labs Inc., Burlingame, CA, USA). Controls for nonspecific binding consisted in omitting the primary antibody (S Fig 5) ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue was then washed with 0.1 M TBS (5 × 5 min) and incubated in ImmPRESS HRP goat anti-rabbit IgG polymer detection kit reagent (Vector Laboratories, MP-74551) for 18 h at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... Cells were then incubated for 30 min at RT with secondary antibody fluorochrome-conjugated anti-Rabbit Dylight 488 (Vector Laboratories, Newark, CA, USA). Finally ...
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bioRxiv - Cell Biology 2022Quote: ... Bound integrins were quantified by an enzyme-linked immunosorbent (ELISA)-like solid-phase assay using biotinylated rabbit anti-velcro (against ACIS/BASE coiled-coil) antibody and HRP-conjugated streptavidin (VECTOR Laboratories #SA-5004). After addition of ABTS ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were blocked with BSA in PBS-Tween and antibodies were incubated at the following dilutions: anti-Biotin (1:1000, Vector Labs, SP-3000), anti-RNA polymerase II CTD repeat YSPTSPS [phospho S5] [4H8] (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... 0.3% Triton X-100 (24 hours, 37°C) followed by peroxidase-conjugated secondary antibody (1:200 biotinylated anti-rabbit Vector Labs #BA-1000) and incubated in Elite ABC solution ...
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bioRxiv - Cancer Biology 2024Quote: ... slides were washed twice with 1x PBS and incubated with ImmPRESS® HRP Horse Anti-Rabbit IgG Polymer Reagent (Vector Laboratories MP-7401) for 30 min at room temperature ...
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bioRxiv - Immunology 2024Quote: ... diluted 1:30 was applied for 15 minutes followed by biotinylated secondary rabbit anti-rat antibody diluted 1:400 (BA-4001; Vector Laboratories, Burlingame, CA) for 10 minutes and then ready-to-use Bond polymer refine detection kit with 3,3’- diaminobenzidine (DAB ...
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bioRxiv - Developmental Biology 2024Quote: ... slides were rinsed with PBS and incubated for 40 minutes at room temperature with horseradish-peroxidase polymer horse anti-rabbit secondary antibody (MP-6401-15, Vector Laboratories, Burlington, ON) in a covered humidity chamber ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed three times in 0.1% Triton X-100 in TBS followed by 1 hr incubation with goat biotinylated anti-rabbit IgG secondary antibody (1:250, BA-1000, Vector Laboratories, RRID: AB_2313606) at room temperature and 30 min incubation with Vectastain Elite ABC reagent (PK-6100 ...
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bioRxiv - Neuroscience 2024Quote: ... slides were washed in PBS 1X and incubated for 45 minutes with goat anti-Rabbit IgG secondary biotinylated antibody (1:500, Vector Laboratories, BA-1000) followed by an incubation with Cy3-conjugated streptavidin (1:300 ...
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bioRxiv - Physiology 2024Quote: ... mouse pancreatic sections were incubated overnight at 4°C in guinea pig anti-insulin antibody (1:800) and visualized using the diaminobenzidine peroxidase substrate (Vector laboratories, Burlingame, CA) and hematoxylin staining ...
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bioRxiv - Cell Biology 2020Quote: ... The embryos were then washed 3 x 10 minutes with 3% BSA in PBS-T and 2 x 10 minutes with PBS alone and mounted on glass slides (Superfrost Plus, Menzel, Braunschweig, Germany) with anti-fade mounting medium (Vectashield, Vector Laboratories, Burlingame, California, USA).
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bioRxiv - Microbiology 2021Quote: ... After blocking of endogenous peroxidases sections were incubated with ImmPRESS® HRP Horse Anti-Rabbit IgG Polymer Detection Kit (Vector Laboratories, MP-7401-15) for 1h at RT and then with DAB substrate (Roche ...
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bioRxiv - Evolutionary Biology 2021Quote: ... DIG-labelled probes were visualized using anti-DIG AP-conjugated antibodies and the Vector Red Alkaline Phosphatase (AP) Substrate Kit (Vector Laboratories, Burlingame, CA, US); biotin-labelled probes were visualized by streptavidin-horseradish peroxidase (HRP ...
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bioRxiv - Molecular Biology 2019Quote: ... Sections were then washed for 2 minutes in PBS and incubated in the dark for 15 minutes with a secondary antibody solution containing Texas Red-conjugated anti-rabbit IgG (catalog #: TI-1000; Vector Laboratories, Burlingame, CA, USA), and Alexa Fluor 488-conjugated anti-mouse IgG (catalog # ...
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bioRxiv - Neuroscience 2020Quote: ... The tissue was then washed in PBS and incubated for 1 hour in biotinylated anti-rabbit IgG (1:200; Vector Labs, Burlingame, CA, USA), rotating at RT ...
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bioRxiv - Immunology 2021Quote: ... for one hour at a 1:600 dilution and then incubated in a rabbit anti-rat secondary (BA-4001, Vector Laboratories, Inc., Burlingame, CA) for 12mins at a 1:7500 dilution ...
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bioRxiv - Neuroscience 2022Quote: ... This was followed by an incubation with biotinylated goat anti-rabbit secondary antibody (1:200 in 3% NGS + 0.1% Triton X-100; Vector Labs, Burlingame, CA; Lot# 140893). Next ...
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bioRxiv - Neuroscience 2022Quote: ... sections were washed 5x in 0.1M PBS for 10 min per wash and incubated overnight at 4°C in secondary antibody (biotinylated goat anti-rabbit IgG; 1:500; Vector Laboratories, Inc, Burlingame, CA). The last day ...
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bioRxiv - Immunology 2022Quote: ... Detection of bound antibodies was performed with biotinylated secondary goat anti-rabbit antibody and ABC-Kit using the peroxidase substrate method DABImmPact (all from Vector Laboratories, Burlingame, CA, USA).
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bioRxiv - Cancer Biology 2022Quote: ... Sections were incubated with ImmPRESS® - HRP anti-goat IgG reagent for 1 hr and developed using ImmPACT® NovaRED or ImmPACT® DAB (Vector Laboratories). Images were captured with a Nikon 80i Eclipse microscope and processed with NIS-Elements Imaging Software.
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bioRxiv - Neuroscience 2019Quote: ... Sections were then rinsed and incubated in secondary antibody solution for 24h at 4°C: 1:1000 rabbit anti-goat (Vector Laboratories, Burlington, ON, Canada) in 0.1M PBS ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated at room temperature for 1.5 hours in secondary antibody solution containing either biotinylated rabbit anti-goat IgG (Vector Laboratories, Burlingame, CA; PK-6105) or biotinylated goat anti-rabbit IgG (Vector Laboratories ...
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bioRxiv - Developmental Biology 2022Quote: ... immunofluorescence was performed on paraffin sections using antibodies in Supplemental Table 1b and the following reagents: ImmPRESS(r) HRP Horse Anti-Rabbit IgG Polymer Detection Kit (Vector Labs, MP-7401-50), ImmPRESS(r ...
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bioRxiv - Cancer Biology 2022Quote: ... Cleaved caspase 8 immunohistochemistry staining was performed using cleaved caspase 8 primary antibody (1:100; Cell Signalling Technology, cat. #9496) and goat anti-rabbit biotinylated secondary (1:200; Vector Laboratories, cat. BA-1000). Briefly ...
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bioRxiv - Neuroscience 2022Quote: ... The tissue sections were incubated overnight at 4°C with primary rabbit anti-TH antibodies followed by staining with biotinylated anti-rabbit IgG and an avidin-biotin peroxidase complex (ABC) standard kit (Vector Laboratories, Burlingame, CA, USA). Signals were detected by incubating sections with 0.5 mg/ml 3,3′-diaminobenzidine (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... Incubation in primary antibody was followed by washes and a 2-hour incubation in anti- rabbit biotinylated secondary antibodies (1:200, Vector Laboratories, Burlingame, CA, RRID:AB_2313606). Sections were then washed and incubated for 1.5 hours in avidin- biotin complex solution (1:200 ...
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bioRxiv - Developmental Biology 2023Quote: ... sections were incubated in Tris-buffered saline/0.1% Tween-20 (TBS-T) containing 2.5% goat serum (included in ImmPRESS HRP Goat Anti-Rabbit IgG Polymer Kit, Vector Laboratories, MP-MP-7451-15) to block non-specific binding ...
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bioRxiv - Microbiology 2023Quote: ... Parasites were then stained with an anti-VSG-2 monoclonal antibody (Hovel-Miner et al., 2012) conjugated to Alexa647 and mounted in Vectashield (Vector Laboratories, H-1800-10) containing 4′,6-diamidino-2-phenylindole (DAPI) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and incubated for 2-3 hours with biotinylated anti-rabbit secondary antibody (1:450 for DeltaFosB and 1:600 for c-Fos, Vector Laboratories, Burlingame, CA, USA). Finally ...
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bioRxiv - Developmental Biology 2021Quote: Immunohistochemistry was performed as previously described (Frei et al., 2011) using anti-HAND2 antibody (A-12, Sc-398167, 1:100) and secondary universal antibody (Vectastain, Vector laboratories Inc., Burlingame, CA, USA), according to manufacturer instructions ...
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bioRxiv - Microbiology 2021Quote: ... was used to stain nucleic acids following washing away unbound secondary antibodies and samples were covered with VectaShield anti-fade reagent (Vector Laboratories, VWR, Cat# 101098-048) and a coverslip ...
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bioRxiv - Neuroscience 2019Quote: ... After overnight incubation in primary antibody,the sections were rinsed in washing buffer and then incubated for 1 h in an anti-rabbit biotinylated antibody (Vector Laboratories, #ba-9400; 1:200) in washing buffer containing 2% normal goat serum ...
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bioRxiv - Pathology 2022Quote: ... overnight at 4°C then rinsed with TBST prior to detection using an anti-rabbit avidin-biotin amplification kit (Vector Laboratories Cat# PK-6101, RRID:AB_2336820). Vector NovaRed ...
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bioRxiv - Neuroscience 2019Quote: ... sections were incubated for 1 hr at room temperature in a biotinylated solution (biotinylated goat anti-rabbit IgG; 1:200 dilution; Vector Laboratories; catalog no. BA-1000) before incubating in an avidin-biotin horseradish peroxidase solution for 2 hrs at room temperature (Vector Novoxastra Laboratories) ...
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bioRxiv - Microbiology 2022Quote: ... Slides were washed thrice with PBS to remove excess antibody and incubated with a rabbit anti-goat biotinylated IgG (Vector laboratories catalogue number BA-5000) for 10 minutes ...
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bioRxiv - Immunology 2023Quote: ... primary antibody in Leica Primary Antibody Diluent for 30 min was followed by application of a rabbit anti-rat secondary (Vector Laboratories, Catalog Number AI-4001) for 8 min ...
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bioRxiv - Developmental Biology 2024Quote: ... immunofluorescence was performed on paraffin sections using antibodies in Supplementary Table 2 and the following reagents: ImmPRESS® HRP Horse Anti-Rabbit IgG Polymer Detection Kit (Vector Labs, MP-7401-50). Following the application of TSA fluorophores (listed in Supplementary Table 2 ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were washed with PBS (3 x 10 min) and incubated with biotinylated goat anti-rabbit secondary antibody (1:600, Vector Laboratories, Cat# BA-1000, RRID:AB_2313606), diluted in 1% NGS in PBS with Triton X-100 ...
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bioRxiv - Pathology 2024Quote: ... the primary antibody was detected using the ImmPRESS® HRP Horse Anti-Rabbit IgG PLUS Polymer Kit (Vector Laboratories, MP-7801-15, Newark, California, USA) following manufacturer instructions.
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Targeting MOG to skin macrophages prevents EAE in macaques through TGFβ-induced peripheral tolerancebioRxiv - Immunology 2019Quote: ... Incubation with primary antibodies was followed by a biotinylated goat-anti-rabbit antibody for 30 min followed by the avidin–biotin–peroxidase complex (Vectastain Elite ABC Kit, Vector Laboratories, PK 6100; Burlingame, CA, USA) for 30 min at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated with a rabbit polyclonal anti-syncollin antibody for 2 h at room temperature followed by an FITC-conjugated goat anti-rabbit secondary antibody and 4′,6-diamidino-2-phenylindole (DAPI, Vector Laboratories, Burlingame, CA; 1.5 μg.mL−1).
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bioRxiv - Neuroscience 2021Quote: ... sections were washed in PBST and incubated for 60 minutes at room temperature with a secondary biotinylated goat anti-rabbit antibody (Vector laboratories, Cat# BA-1000, lot# 2F0430) diluted 1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... the samples were washed twice for 20 min in 2XSSC at 42°C and subsequently incubated with fluorescein anti-biotin antibody (Vector Laboratories, SP-3040; Dilution 1:100) for at least 45min ...
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bioRxiv - Molecular Biology 2023Quote: ... slides were incubated for 1 hour with a secondary antibody cocktail: 1:250 anti-rabbit IgG Texas Red 594 (Vector Labs, Newark, CA, USA; TI-1000) + anti-mouse IgG2b AF488 (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... The next day the slides were rinsed 3 times for 5 minutes in 1X PBS and then incubated for 1 hour at RT with anti-rabbit biotinylated secondary antibody (Vector Laboratories, product no: BA-1000 (1:500)) diluted in blocking solution ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein block and secondary antibody for CD3 employed ImmPRESS-Alkaline Phosphatase Horse Anti-Rabbit IgG Polymer Kit-Alkaline Phosphatase (Vector Laboratories, Burlingame, CA, Cat No. MP-5401). F4/80 antibody (1:200 ...