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Citations for Vector Labs :
1301 - 1350 of 1492 citations for RGPD1 2 3 4 5 8 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... slides were washed 5 times with 1X PBS prior to being mounted with VectaShield medium containing DAPI (Vector lab, Burlingame, CA).
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bioRxiv - Evolutionary Biology 2022Quote: ... The samples were washed three times in PBTx for 15 min followed by five 5-minute washes in PBS and mounted in Vectashield antifade mounting medium containing DAPI (Vector Laboratories). Samples were imaged on an Olympus FV3000 Confocal Laser Scanning Microscope and processed in Imaris.
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bioRxiv - Microbiology 2022Quote: ... The membrane was washed 6 separate times in TBS-T for 5 min and then incubated with HRP-conjugated streptavidin (Vector Laboratories) for an additional hour ...
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bioRxiv - Cell Biology 2022Quote: ... tissues were washed with PBS (20 min x 5) and incubated with the secondary biotinylated antibodies (1:1000 in PBS, Vector Labs) for 2 hrs at RT ...
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bioRxiv - Molecular Biology 2024Quote: ... Then the fixed specimens were washed thrice for 5 min in 1x phosphate buffered saline (1xPBS; pH 7.4) and mounted using Vectashield mounting medium (H-1000, Vector Laboratories, CA). FITC-albumin and 10 kD dextran specimens were imaged using a ZEISS LSM900 confocal microscope with ZEISS Zen acquisition software (blue edition ...
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bioRxiv - Cell Biology 2024Quote: ... cells were stained with 0.5 μg/mL DAPI in PBS for 5 min prior to drying with ethanol and were mounted using Vectashield mounting medium without DAPI (Vector Laboratories). Images were acquired and analysed using a Leica fluorescence microscope ...
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bioRxiv - Neuroscience 2023Quote: ... Microglia were eliminated by panning on plates coated with Griffonia (Bandeiraea) simplicifolia lectin 1 (5 μg/ml) (Vector Laboratories; L-1100). OPCs were purified on plates coated with an antibody to platelet-derived growth factor receptor alpha (PDGFrα ...
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bioRxiv - Neuroscience 2023Quote: ... The CST was labeled using the Avidin Biotin Complex method (ABC) to detect the BDA by incubating in ABC (5 µL/mL A and B; PK-6100; Vector Laboratories) overnight at room temperature and co-labeled against GFAP (1:5000 ...
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bioRxiv - Neuroscience 2023Quote: ... Incubation with the biotinylated secondary antibody (1:150, Vector in 5% NGS in PBST) for 1 h was followed by the Vector ABC Kit (Vector Laboratories, solution A and solution B in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... tissue sections were washed twice in PBS (5 min) and mounted using Vectashield without DAPI (Vector Laboratories Inc., Burlingame, CA, USA) for immediate visualization by microscopy.
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bioRxiv - Cancer Biology 2023Quote: ... Ki-67 immunohistochemistry (IHC) was performed on 5 μm sections using a mouse on mouse immunodection kit (Vector Laboratories BMK-2202). Briefly ...
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bioRxiv - Pathology 2023Quote: ... The sections were washed with PBST (3x, 5 min) and developed using a DAB substrate kit (Vector Laboratories, Burlingame, CA, USA). Counterstaining was performed with hematoxylin ...
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bioRxiv - Cell Biology 2022Quote: ... Organs were washed three times in PBS for 5 min per wash and mounted in Vectashield (Cat# H-1000, Vector Laboratories).
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bioRxiv - Biochemistry 2024Quote: ... 250 μM CuSO4 and 2.5 mM sodium ascorbate were mixed as a reaction pre-mix with 100 μM of alkyne-label: TAMRA-alkyne (Vector Labs), biotin-PEG4-alkyne (Sigma) ...
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bioRxiv - Cancer Biology 2024Quote: ... membranes were washed in 1X TBST 5 times and incubated with horseradish peroxidase (HRP)-conjugated secondary antibody (1:1000 in 1X TBST) (Vector Laboratories) for 1 hour ...
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bioRxiv - Developmental Biology 2019Quote: ... Secondary antibodies were used for 2 hours at room temperature and then mounted on slides in Vectashield (Vector Labs). Images were taken with a Leica SP5 confocal using 40x oil immersion objective and processed with Adobe Photoshop and ImageJ.
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bioRxiv - Neuroscience 2019Quote: ... They were then treated for 2 h at room temperature with biotinylated secondary antibody (Vector Laboratories, VECTASAIN ABC Kit). Washing between steps was rinsed with PBS ...
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bioRxiv - Neuroscience 2020Quote: ... filled with a chloride solution 0.5 M containing neurobiotin tracer (1-2%, w/v; CAT#SP-1120, Vector laboratories) to perform juxtacellular labelling ...
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bioRxiv - Cancer Biology 2019Quote: ... tissue slides were transferred in deionized water for 2 minutes then stained in Eosin for 30 second (Vector Labs) followed by three incubations of 5 min in 95% ethanol then 100% ethanol ...
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bioRxiv - Neuroscience 2021Quote: Reagents used were as follows: N-(2-aminoethyl) biotin amide hydrochloride (NEUROBIOTIN™ Tracer, SP-1120, from Vector Laboratories), Alexa 488-conjugated streptavidin (Molecular Probes ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... Sections were then incubated at room temperature for 2 h in biotinylated goat anti-mouse (1:200, Vector Laboratories). Brain sections were further processed using the Avidin-Biotin Complex (ABC Elite kit ...
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bioRxiv - Neuroscience 2022Quote: ... followed by 2 h incubation in a solution containing avidin-biotin-peroxidase complex (ABC, 1:300 dilution, Vector Laboratories). Finally ...
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bioRxiv - Neuroscience 2022Quote: ... followed by 2 h incubation in a solution containing avidin–biotin–peroxidase complex (ABC, 1:300 dilution, Vector Laboratories). Finally ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated for 2 h at room temperature with species-specific biotinylated secondary antibody (1:200 dilution) (Vector Laboratories) in 0.1M PBS ...
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bioRxiv - Developmental Biology 2024Quote: ... containing 0.3% Tween-20 and blocked with PBS with 0.3% Triton-X100 and 2% normal hose serum (Vector Laboratories # S-2000 ...
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bioRxiv - Cell Biology 2023Quote: ... and a hydrophobic barrier was created around the tissue using a hydrophobic barrier pen (Vector Laboratories, H-4000-2). For immunohistochemical (IHC ...
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bioRxiv - Neuroscience 2023Quote: ... The electrodes were filled with 0.5 M K+- acetate and 2% neurobiotin (Vector Laboratories, Burlingame, CA, USA, #SP-1120). Juxtacellular labeling of the recorded neurons was done by filling the neurons with neurobiotin ...
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bioRxiv - Genetics 2024Quote: ... Tissue sections were blocked with 2.5% normal donkey serum and 0.1% 4’6-diamidino-2-phenylindole (DAPI, Vector lab, D21490) dissolved in 0.2% PBST for 30 mins after washing with PBS three times ...
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bioRxiv - Bioengineering 2021Quote: ... Mounting was conducted by incubating for 16 h at 4 °C with VECTASHIELD® PLUS Antifade Mounting Medium (H-1900; Vector Laboratories, Burlingame, CA). Extended information detailing primary antibody ...
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bioRxiv - Neuroscience 2022Quote: ... sections were washed 5x in 0.1M PBS for 10 min per wash and incubated overnight at 4°C in secondary antibody (biotinylated goat anti-rabbit IgG; 1:500; Vector Laboratories, Inc, Burlingame, CA). The last day ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were then rinsed and incubated in secondary antibody solution for 24h at 4°C: 1:1000 rabbit anti-goat (Vector Laboratories, Burlington, ON, Canada) in 0.1M PBS ...
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bioRxiv - Microbiology 2022Quote: ... The cells were washed twice with DPBS before the coverslips were mounted on to a glass slide with 4 μl Vectashield with DAPI (Vector Laboratories, cat# H-1200) and sealed with clear nail polish ...
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bioRxiv - Cancer Biology 2023Quote: ... Autofluorescence in tissue sections due to aldehyde fixation was removed by treatment with the TrueVIEW Autofluorescence Quenching Kit for 4 min (Vector Laboratories; SP-8400-15). Sections were counterstained with DAPI (ThermoFisher 62247 ...
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bioRxiv - Microbiology 2022Quote: ... cells were stained at room temperature with 5 μg/mL fluorescein-conjugated wheat-germ agglutinin (WGA, Vector Laboratories catalog no. FL-1021) for 15 minutes to detect exposed chitin or 50 μg/mL fluorescein-conjugated concanavalin A (ConA ...
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bioRxiv - Neuroscience 2021Quote: Free-floating parallel brain sections were washed with PBS + 0.5% Triton X-100 (PBS-T) and then blocked with 5% normal goat serum (NGS, #S1000, Vector laboratories, Burlingame, CA) in PBS-T for 1 h ...
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Neuron-specific knockouts indicate the importance of network communication to Drosophila rhythmicitybioRxiv - Neuroscience 2019Quote: ... the brains were rinsed 5 × 10 mins with PBST and mounted on glass slides using Vectashield (Vector Laboratories INC., Burlingame, CA, USA) mounting medium.
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bioRxiv - Cell Biology 2021Quote: ... membranes were washed 4 × 10min in TBS 0.1% Tween and incubated with one of the following peroxidase-conjugated secondary antibodies diluted in TBS 0.1% Tween supplemented with 5%BSA for 2h at room temperature: horse anti-mouse IgG(H+L) (Vector laboratories, PI-2000), goat anti-rabbit IgG(H+L ...
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bioRxiv - Neuroscience 2020Quote: ... The peroxidase reaction was produced by incubating the sections for 5 to 12 minutes in a DAB substrate solution (SK-4100; Vector Labs, CA). Reacted tissue was then mounted on gelatin coated slides ...
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bioRxiv - Neuroscience 2019Quote: ... slices were washed for 3 x 5 minutes in 0.1 M PBS and mounted on glass slides using Vectashield medium (containing DAPI as required, Vector Labs, Peterborough, UK) and cover-slipped.
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bioRxiv - Cell Biology 2020Quote: ... DAPI was applied as a nuclear counter stain at 0.3 μM for 5 minutes prior to mounting with medium (Vectashield soft, Vector Labs, Peterborough, UK) and placing the coverslips upside down onto microscope slides ...
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bioRxiv - Cancer Biology 2020Quote: ... was used at 1:100 for 45 minutes at room temperature followed by a 5-minute incubation with Vectastain® ABC kit (Vector laboratories). 3,3’ diaminobenxidine (DAB ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were permeabilized with 0.5% Triton X-100 for 30 min at room temperature and blocked in 5% goat serum (Vector Laboratories, 1:200), goat anti-rat 488 and goat anti-mouse 647 (Invitrogen ...
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bioRxiv - Physiology 2020Quote: ... Sections were washed with PBS for 10 min before staining with 1:1000 dilution of rhodamine wheat germ agglutinin (WGA; 5 μg/mL;Vector Laboratories,UK) for 10 min ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were washed 5 times again and mounted onto slides using Vectashield Hardset Antifade reagent with DAPI (Vector Labs, cat. # H-1400). Thalamocortical slice imaging was performed on an Andor Dragonfly microscope described below.
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bioRxiv - Molecular Biology 2021Quote: ... Sections were washed with PBS for 10 min before staining with 1:1000 dilution of rhodamine wheat germ agglutinin (WGA; 5 μg/mL;Vector Laboratories, UK) for 10 min ...
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bioRxiv - Neuroscience 2023Quote: ... we removed the supernatant and added 1 ml ACSF with DAPI (Vector Laboratories, Cat No.: H-1200-10ml, 5 μ g/ml). Collected single-cell suspensions were used for library construction.
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bioRxiv - Developmental Biology 2024Quote: ... Slides were washed twice in PBS for 5 min and mounted on a drop of VECTASHIELD solution (Vector Laboratories, H-1200-10). Images were acquired using a Zeiss LSM 880 confocal microscope.
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bioRxiv - Genetics 2024Quote: ... Cover slips were subsequently washed with Wash buffer B for 5 minutes and mounted onto the slides with Vectashield® Mounting Medium (H-1000, Vector Laboratories). Images were taken at the Imaging Facility ...
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bioRxiv - Microbiology 2024Quote: ... the specimens were washed in PBS for 5 min each and cover with glass slides using mounting medium (Vector Laboratories, United States). For imaging ...
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bioRxiv - Neuroscience 2022Quote: ... or Phaseolus vulgaris leucoagglutinin (PHA-L; 2.5% solution in 10 mM phosphate buffer, pH 7.4; Vector Laboratories, Cat. No. L-1110-5). To reduce the number of animals ...