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Citations for Vector Labs :
1251 - 1300 of 1305 citations for 7H Pyrrolo 2 3 d pyrimidin 2 amine 7 3 5 bis O 2 4 dichlorophenyl methyl 2 C methyl b D ribofuranosyl 4 chloro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Sections were washed 5 times again and mounted onto slides using Vectashield Hardset Antifade reagent with DAPI (Vector Labs, cat. # H-1400). Thalamocortical slice imaging was performed on an Andor Dragonfly microscope described below.
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bioRxiv - Neuroscience 2019Quote: ... followed by blocking at room temperature for 30 minutes in blocking solution containing 5% normal goat serum (NGS) (Vector Labs, Burlingame, CA), 0.1% Triton X100 ...
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bioRxiv - Molecular Biology 2021Quote: ... Sections were washed with PBS for 10 min before staining with 1:1000 dilution of rhodamine wheat germ agglutinin (WGA; 5 μg/mL;Vector Laboratories, UK) for 10 min ...
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bioRxiv - Neuroscience 2023Quote: ... we removed the supernatant and added 1 ml ACSF with DAPI (Vector Laboratories, Cat No.: H-1200-10ml, 5 μ g/ml). Collected single-cell suspensions were used for library construction.
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bioRxiv - Neuroscience 2023Quote: ... The sections were incubated at 4 °C overnight with primary antibody diluted in PBS containing 0.3% Triton X-100 and 5% normal goat serum blocking solution (Vector Laboratories, #S-1000). Anti-mouse monoclonal pS202/pT205 1:1000 (AT8 ...
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bioRxiv - Microbiology 2024Quote: ... the specimens were washed in PBS for 5 min each and cover with glass slides using mounting medium (Vector Laboratories, United States). For imaging ...
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bioRxiv - Developmental Biology 2024Quote: ... Slides were washed twice in PBS for 5 min and mounted on a drop of VECTASHIELD solution (Vector Laboratories, H-1200-10). Images were acquired using a Zeiss LSM 880 confocal microscope.
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bioRxiv - Neuroscience 2023Quote: ... Slides were blocked/permeabilized for 1 hour at room temperature with a solution of 5% Normal Horse Serum (Vector Laboratories, Burlingame, California), 0.2% Triton X-100 (Amresco ...
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bioRxiv - Neuroscience 2022Quote: ... or Phaseolus vulgaris leucoagglutinin (PHA-L; 2.5% solution in 10 mM phosphate buffer, pH 7.4; Vector Laboratories, Cat. No. L-1110-5). To reduce the number of animals ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were washed 4 × 10min in TBS 0.1% Tween and incubated with one of the following peroxidase-conjugated secondary antibodies diluted in TBS 0.1% Tween supplemented with 5%BSA for 2h at room temperature: horse anti-mouse IgG(H+L) (Vector laboratories, PI-2000), goat anti-rabbit IgG(H+L ...
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bioRxiv - Neuroscience 2023Quote: ... slides were deparaffinized and boiled for 20 min in citrate buffer (0.1M sodium citrate and 0.1M citric acid in distilled H2O) for antigen retrieval and incubated with 5% normal rabbit serum (Vector laboratory #S-5000) in PBS containing 0.2% Triton for 1 h at RT to block unspecific binding ...
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bioRxiv - Cancer Biology 2024Quote: Immunohistochemistry on 5 µm sections from frozen or paralin-embedded tissues was performed using the biotin-peroxidase method (Vector laboratories, Burlingame, CA). IHC staining and scoring were evaluated by a trained pathologist.
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bioRxiv - Cancer Biology 2024Quote: ... Sections were washed with 1x PBS for 5 min and antigen retrieval was performed with Antigen Unmasking Solution Citrate-based pH 6.0 (Vector Laboratories H-3300) in a Decloaking Chamber™ (Biocare Medical ...
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bioRxiv - Cell Biology 2024Quote: ... After blocking with 5% goat serum/1% bovine serum albumin/PBS and Mouse on Mouse blocking reagent (Vector Laboratories, Newark, CA, USA), the samples were incubated with primary antibodies overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... incubated in 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked in 5% normal goat serum (NGS) (Vector Labs, Burlingame, CA) with 0.1% Triton X-100 ...
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bioRxiv - Synthetic Biology 2021Quote: ... blocked in 5% bovine serum albumin for 30 min and probed for 1 h with fluorescein-labeled SBA (Vector Laboratories, catalog # FL-1011) and Alexa Fluor 647® (AF647)-conjugated anti-His antibody (R&D Systems ...
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bioRxiv - Cell Biology 2021Quote: ... the retinal sections were washed with PBS three times for 5 min each and then mounted with Vectashield Mounting Medium (Vector Laboratories, Burlingame, CA). The images were acquired with a BZ-X810 Keyence microscope (Keyence ...
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bioRxiv - Physiology 2021Quote: ... Sections were incubated at room temperature with 3% (v/v) H2O2 for 10 min to quench endogenous peroxidase activity and blocked by 5% (v/v) normal goat serum (Vector Laboratories, #S-1000) in PBS for 1 hour ...
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Mechanotransduction-dependent control of stereocilia dimensions and row identity in inner hair cellsbioRxiv - Cell Biology 2019Quote: ... Organs were washed three time in PBS for 5 min per wash and then mounted in Vectashield (Cat#H-1000, Vector Laboratories, Burlingame, CA).
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bioRxiv - Neuroscience 2020Quote: ... Another set of washes in PBS (3x–5 min each) were performed and then the slides were mounted using Vectashield with DAPI (Vector Labs, H-1200).
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bioRxiv - Immunology 2020Quote: ... 3 times with PBTD and counterstaining with DAPI in 5% NGS blocking solution took place after the first wash. Coverslips (No. 1) were mounted using Vectashield (Vector Laboratories, Burlingame, California) and sealed with nail polish ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then rinsed in PBS (5 x 5 min) followed by a 1 hour incubation in biotinylated goat anti-rabbit secondary (1:500 in PBS, Vector Laboratories, BA-1000). Sections were rinsed in PBS (5 x 5 min ...
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bioRxiv - Molecular Biology 2022Quote: ... ATAC-see samples were incubated for 1 hour at room temperature protected from light with blocking buffer (1X PBS, 0.1% TX-100 and 5% normal goat serum (Vector Laboratories, S-1000-20). ds/ssDNA mouse monoclonal IgM antibody clone AC-30-10 (PROGEN ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed with PBS-TX (0.5% Triton X-100 in PBS) 6 x 20 min then blocked in 5% normal goat serum (NGS; Vector Laboratories, Burlingame, CA, USA) for 1 h before being incubated on a shaker overnight with primary antibodies (Table 1 ...
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bioRxiv - Neuroscience 2024Quote: ... slides were rinsed three times for 5 minutes with 0.4% Triton-X-100 TBS and mounted using VectaShield® Hardset ™ Antifade Mounting Medium with DAPI (Vector Laboratories, California). Slides were then left alone to set for at least 48 hours at 4° C and imaged on a Leica DMi8 Confocal Microscope.
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bioRxiv - Cancer Biology 2023Quote: ... Cell nuclei were stained with spectral DAPI in PBS for 5 min and the slides were mounted with Hard-set Vectashield mounting medium (Vector Laboratories, Burlingame, CA). Mounted slides were allowed to harden prior to scanning.
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... followed by washing 3x in PBS for 5 minutes before 60-minute incubation with a Streptavidin-conjugated Dylight 488 secondary (1:200, Vector Laboratories, SA-5488). After secondary ...
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bioRxiv - Bioengineering 2023Quote: ... Slides were washed with 1X TBS twice again for 5 minutes each and Vectashield Mounting Media (H-1200; Vector Laboratories, Newark, CA, USA) was added before coverslipping ...
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bioRxiv - Neuroscience 2023Quote: ... The intracellular single unit recordings were performed on the L2/L3 DRGs using the quartz microelectrode containing 5% Neurobiotin (Vector Laboratories, Burlingame, CA) in 1M potassium acetate ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Haematoxylin and Eosin (H&E) staining was performed on 5-μm sections that were prepared and stained using kit (Vector Laboratories, CA, USA) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... We washed slides twice for 5 minutes in RNase-free 1X PBS and marked the sample perimeter of each slide with an ImmEdge pen (Vector Laboratories #H-4000) in between both washes ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were washed with PBS and either stained for 5 min with 1 µg/ml DAPI (for chamber slides) or mounted using DAPI-containing Vectashield (Vector Laboratories; for coverslips). An Olympus IX70 inverted microscope with a UPlanApo 100X/1.35 oil iris objective was used for image acquisition with Micro-Manager software (v2.0) ...
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bioRxiv - Molecular Biology 2021Quote: All slides were washed three times for 5 min at RT and dried before mounting with the DAPI-containing VectaMount Permanent Mounting Medium (Vector Laboratories, Burlingame, CA, USA), and sealed with nail varnish ...
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bioRxiv - Developmental Biology 2020Quote: ... Secondary antibody was decanted and slides washed three times with PBS for 5 minutes each followed by VECTASHIELD® Antifade Mounting Medium with DAPI (Vector Labs H-1200), sealing coverslip ...
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bioRxiv - Neuroscience 2021Quote: ... sections were washed two more times for 5 min in 1x PBS and mounted with Vectashield mounting media (Vector Laboratories, # H1000, Burlingame, CA, USA). The following primary antibodies and dilutions were used ...
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bioRxiv - Biochemistry 2020Quote: ... The membranes were blocked with 5% BSA and then incubated with biotin-labeled Maackia amurensis lectin II (MAL-II biotin conjugate, Vector Laboratories, Inc., Burlingame, CA) for 1 h at room temperature ...
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bioRxiv - Microbiology 2021Quote: Paraffin-embedded tissues were sectioned at 5 µm and subjected to IHC using Vectastain Elite ABC Peroxidase (HRP) Kit (Vector Laboratories Cat # PK-6102) as previously described [40] ...
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bioRxiv - Immunology 2022Quote: Clarified cell supernatant was adjusted to a final concentration of 100 mM NaCl and then incubated with Galanthus Nivalis Lectin Agarose (Vector Laboratories, AL-1243-5), washed with 10 column volumes of 20 mM Tris ...
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bioRxiv - Cell Biology 2022Quote: ... Endogenous peroxidase was blocked for 5 mins before incubation with 2.5% Normal Goat Serum (ImmPRESS Rat kit – Vector Laboratories, San Francisco Bay Area, USA) TRU for 20 mins ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were washed three times with 1X PBS for 5 minutes each wash and then mounted using a DAPI-staining mounting medium (Vector Laboratories; H-1200-10). Cells were imaged using Leica DMI4000B microscope fitted with Leica TCS SPE laser-scanning confocal system with Solid State lasers (405/488/561/635 ...
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bioRxiv - Microbiology 2021Quote: ... Worms were washed in PBS three times for 5 mins and transferred to new 12 well plates with 1ml of Fluorescein labeled Aleuria Aurantia Lectin (Vector labs cat# FL-1391) and Rhodamine labeled Griffonia Simplicifolia Lectin I (Vector labs cat# RL-1102 ...
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bioRxiv - Neuroscience 2022Quote: ... antigen retrieval was performed by using either formic acid for 5 minutes at room temperature or citric acid pH=6 (Vector Laboratories; Cat# H-3300) for 15 minutes at 95 °C ...
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bioRxiv - Neuroscience 2023Quote: ... The blots were washed with 1X TBST thrice for 5 minutes and then incubated with HRP-conjugated secondary antibodies (1:4000, Vector Labs, Burlingame, CA, USA) for 1-2 hours at RT ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... the slides were washed three times with PBS (5 minutes each at RT) and mounted using Vectashield antifade mounting media with DAPI (Vector Laboratories, #H-1200-10). To secure the coverslips ...
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bioRxiv - Neuroscience 2024Quote: ... sections were rinsed in PBS three times for 5 min each and mounted on slides with VECTASHIELD Antifade Mounting Medium with DAPI (Vector Laboratories, H-1200-10) and coverslipped ...
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bioRxiv - Neuroscience 2021Quote: ... and then cleared in xylene for 5 min before mounting with antifade medium with DAPI (VectaShield® HardSet™ with DAPI, Vector Laboratories, Auckland, NZ) and a coverslip ...
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bioRxiv - Immunology 2020Quote: ... The SARS-CoV-2 RBD-His tag and S1 proteins were purified using Ni-NTA resin (Thermofischer, cat.no. 88221) and Agarose bound Galanthus Nivalis Lectin (GNL) (Vector Labs, cat. no. AL-1243-5) respectively ...
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bioRxiv - Immunology 2023Quote: Single chain HIV-1 gp140 SOSIP variant strain BG505 [52] was purified over agarose bound Galanthus nivalis lectin (Vector Laboratories cat no. AL-1243-5). Column was washed with PBS ...
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bioRxiv - Neuroscience 2019Quote: ... the far end of the pallial nerve was placed in a Vaseline pool filled with tracer solution (Neurobiotin 5% in distilled water, Vector Laboratories Cat #SP-1120, RRID: AB_2313575) while the remaining tissue was immersed in sea water ...
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bioRxiv - Immunology 2021Quote: ... Supernatant was 0.45 µm filtered with PES membrane Nalgene Rapid Flow Disposable Filter Units and then run slowly over an affinity column of agarose bound Galanthus nivalis lectin (Vector Laboratories cat no. AL-1243-5) at 4°C ...