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Citations for Vector Labs :
1101 - 1150 of 1384 citations for GDNF family receptor alpha 1 GFRA1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... were applied for 1 h at 37°C prior to mounting onto slides using mounting media containing DAPI (Vectashield, Vector Labs). Images were obtained on a Leica SP5 confocal microscope or a Nexcelom Celigo as described in individual figure legends.
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bioRxiv - Neuroscience 2020Quote: ... 30 min in biotinylated goat anti-mouse or anti-rabbit IgG (1:200, PA-920 or BA-1000; Vector Laboratories, USA); 30 min avidin-biotin complex solution (PK-7100 ...
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bioRxiv - Neuroscience 2020Quote: ... Tissue was rinsed five times for ten minutes in PBS and then incubated overnight at 4°C in a rabbit anti-goat secondary (1:500; Vector Laboratories) diluted in PBS ...
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bioRxiv - Microbiology 2020Quote: ... The homogenate was blocked using 3% bovine serum albumin (BSA) in 0.2% Triton X-100 before staining with 1:250 rhodamine-conjugated Dolichos biflorus lectin (Vector Laboratories Inc.) to visualize the cyst wall ...
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bioRxiv - Immunology 2021Quote: ... Germany) for 60minutes at a 1:1500 dilution and followed by a biotinylated anti-rat (Cat. BA-5000, Vector Laboratories, Inc.) for 15 minutes at a 1:2000 dilution ...
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bioRxiv - Neuroscience 2022Quote: ... tissues were incubated for 1-hour in an avidin biotin complex solution (per kit instructions, ABC Peroxidase, PK-6100, Vector Laboratories) at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... PV staining was performed as above and then sections were incubated at 4°C overnight with byotinilated VVA (1:5000, #B-1235, RRID: AB_2336855, Vector Laboratories, USA). After rinsing ...
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bioRxiv - Neuroscience 2022Quote: ... in 0.1 M PB, for 10 min) to reduce background auto-fluorescence (Clancy & Cauller, 1998) before being mounted in VectaShield (Vector Laboratories) and later imaged using an epifluorescence system (Leica DM 5000 ...
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bioRxiv - Neuroscience 2022Quote: ... incubated in appropriate secondary antibody solutions for 2 hours (ChAT (1:200): rabbit anti-goat IgG (biotinylated; Vector Laboratories, BA-5000); PV (1:200) ...
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bioRxiv - Neuroscience 2022Quote: ... staining was enhanced after primary antibody incubation with biotinylated secondary antibodies (biotinylated horse anti-goat IgG, Vector Laboratories: BA-9500, RRID: AB_2336123; 1:300; biotinylated goat anti-rabbit – bGAR, Vector Laboratories: BA-1000 ...
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bioRxiv - Neuroscience 2022Quote: ... all brain sections were transferred into the secondary antibody solution (1:200 in PBS; biotinylated anti-rabbit made in goat, BA-1000 Vector Laboratories) for 60 min at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... or CD4 (ab183685, 1:400 dilution overnight at 4 degrees).Tissues were then counterstained with biotinylated anti-rabbit secondary (Vector Labs, BA-1000 ...
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bioRxiv - Genetics 2022Quote: ... Stained gonads were resuspended in 1 drop of Vectashield containing 4’,6-Diamidino-2-Phenylindole Dihydrochloride (DAPI) (Vector Laboratories H-1200), applied to a large pre-made agarose pad on a glass slide ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated with primary antibody for 16-24 hrs at 4°C and then for 2 hrs at 4°C with biotinylated goat anti-rabbit IgG secondary antibody (1:800, Vector Laboratories) in 5% normal goat serum in 0.1% Triton-X-100 in TBS ...
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bioRxiv - Neuroscience 2020Quote: ... After the transfer the blots were blocked for 2 h in 1 x Animal-Free Blocker (SP-5030 Vector Labs, USA). The blots were incubated over night at 4°C under gentle agitation in the primary antibody solutions (1:300 goat anti-UCP1 ...
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bioRxiv - Immunology 2020Quote: RBC ghosts were suspended in equal volumes of calcium binding buffer containing 2% Triton X-100, pre-cleared with magnetic streptavidin beads (88816, Pierce) and incubated with biotinylated GNA lectin (1 mg/ml; B1245, Vector Laboratories), biotinylated MAL-II lectin (1 mg/ml ...
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bioRxiv - Neuroscience 2021Quote: ... Immunoperoxidase detection was then performed by enzymatic reaction with an avidin-peroxidase complex for 1 h (Vectastain ABC HRP Kit Peroxidase Standard, Vector Lab.). After washing in PBS ...
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bioRxiv - Microbiology 2020Quote: ... at 1:200 for 30 minutes followed by Vector Streptavidin Alkaline Phosphatase at a dilution of 1:200 for 20 min (Vector Laboratories). Slides were developed with Bio-Red (Biopath Laboratories ...
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bioRxiv - Microbiology 2020Quote: ... at 1:200 for 30 minutes followed by Vector Streptavidin Alkaline Phosphatase at a dilution of 1:200 for 20 min (Vector Laboratories). Slides were developed with Bio-Red (Biopath ...
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bioRxiv - Cancer Biology 2019Quote: ... slides were thoroughly washed and incubated for 1 h with the appropriate secondary antibody: biotinylated anti-rabbit (BA-1000, Vector Laboratories) or biotinylated anti-mouse (BA-2000 ...
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bioRxiv - Microbiology 2021Quote: ... Triton X-100 in PBS for 15 min at room temperature and immunostained using the SARS-CoV N protein cross-reactive monoclonal antibody 1C7C7 (1 μg/ml) and the Vectastain ABC kit (Vector Laboratories), following the manufacturers’ instruction ...
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bioRxiv - Bioengineering 2021Quote: ... Sections were then washed thrice in phosphate buffered saline prior to 30 min incubation with biotin-conjugated Goat Anti-Mouse IgG (H+L) (1:500, BP-9200, Vector Laboratories). Endogenous peroxidase was blocked and detection was performed using VECTASTAIN® ABC-HRP Kit with signal development carried out using 3,3-diaminobenzidine ...
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bioRxiv - Physiology 2022Quote: ... After reducing non-specific binding with 30 min of Animal-Free Blocker incubation followed by 1 h incubation with Mouse on Mouse Blocking Reagent (Vector Laboratories), sections were incubated with primary antibodies (Proinsulin ...
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bioRxiv - Physiology 2019Quote: ... at a dilution of 1:25 for 20 min using agitation followed by 3 washes with PBT and 1 wash in PBS before mounting in Vectashield with DAPI (#H-1200, Vector Laboratories). Analysis of the tissue and image acquisition was carried out using a Zeiss LSM 710 confocal laserscanning microscope and processed using Fiji and Adobe Photoshop.
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bioRxiv - Molecular Biology 2020Quote: ... cells were stained with 1 µg/ml DAPI and mounted with VECTASHIELD® Antifade Mounting Medium (#H-1000-10, Vector Laboratories). Images were taken using a Perkin Elmer spinning disc confocal equipped with a Nikon-TiE inverted microscope using 60X objective oil lens with an Orca ER CCD camera ...
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bioRxiv - Neuroscience 2020Quote: ... Primary and secondary antibodies were incubated for >36h at 4°C in wash buffer (PBS 1% BSA) and mounted in Vectashield (Vector Labs). Antibodies used were rabbit and guinea pig anti-Hth (1:500 ...
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bioRxiv - Synthetic Biology 2021Quote: ... slides were again washed 5 times for 10 min with 1 × PBS at room temperature and mounted in Vectashield (Vector Laboratories) with 1 µg/ml DAPI ...
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bioRxiv - Microbiology 2021Quote: ... Triton X-100 in PBS for 15 min at room temperature and immunostained using the SARS-CoV cross-reactive N protein 1C7C7 MAb (1 μg/ml) and the Vectastain ABC kit (Vector Laboratories), following the manufacturers’ instruction ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... was used at 5 μg/mL final concentration for 1 hour at 24°C following by goat anti-rabbit IgG biotin conjugate used as secondary antibody at 1-200 final dilution (BA-1000, Vector Laboratories). DAB Map including 3,3-Diaminobenzidine chromogen was applied to the slides for histological staining.
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bioRxiv - Neuroscience 2019Quote: ... sections were incubated with biotinylated anti-goat antibody IgG produced in rabbit (1: 1000; Alexa 594, Vector Laboratories, Burlingame, CA, USA) for 1 hour ...
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bioRxiv - Neuroscience 2019Quote: ... The sections were then incubated in ABC (PK 6100, Vectors Laboratories, Burlingame, CA) for 1 hr and oxytocin-ir visualized using Vector-SG (SK-4700; Vector Laboratories). Sections were mounted onto microscope slides and coverslipped ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then washed five times for ten minutes in PBS and then incubated in an avidin-biotin horseradish peroxidase complex (1:1000; ABC Vector Elite kit; Vector Laboratories) for 4 h at room temperature ...
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bioRxiv - Immunology 2021Quote: ... A secondary biotinylated goat-anti rabbit antibody was used (#BA-1000, diluted 1:200 in TBS, Vector Laboratories, Burlingame, CA, USA) for 30 minutes at RT ...
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bioRxiv - Neuroscience 2019Quote: ... sections were incubated with secondary antibodies for 1 h at RT then washed further in PBS and mounted in Vectashield containing DAPI (Vector Laboratories). Primary antibodies used were ...
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bioRxiv - Developmental Biology 2019Quote: ... Stage 28 hpf embryos were incubated with anti-DIG to detect the nkx2.4b probe and stage 55 hpf embryos were incubated with anti-DNP antibody conjugated to alkaline phosphatase (1:500, Vector Laboratories) to detect tg probe ...
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bioRxiv - Neuroscience 2019Quote: ... the tissue was incubated for 40 min in biotinylated secondary anti-rabbit antibody made in goat (1:200; Vector BA-1000) followed by incubation with the avidin–biotin complex solution (Vectastain ABC kit, Vector Laboratories). Immunoreactivity was visualized using standard DAB procedures ...
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bioRxiv - Neuroscience 2019Quote: ... for 1 hour at room temperature and developed with 3,3’-diaminobenzidine (DAB/ImmPACT DAB) or Vector SG (Vector Laboratories, Burlingame, CA) of for 5-15 minutes (until optimal differentiation of signal) ...
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bioRxiv - Cell Biology 2020Quote: Plasma membranes were labelled by incubating cells with 1:250 wheat germ agglutinin (WGA, v/v) conjugated with the fluorophore FITC or rhodamine (Vector Labs), either live in complete media (immediately after staining to avoid WGA-induced cytotoxicity ...
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bioRxiv - Physiology 2021Quote: ... slides were placed in a blocking buffer (1:60 normal goat serum [Vector Laboratories; Cat. #S-1000] in 0.1% BSA/PBS) for 30 min at RT ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were again washed 3 times in PBST and incubated with FITC- or Texas Red-conjugated streptavidin in blocking buffer (1:50, Vector Laboratories). Where appropriate ...
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bioRxiv - Cancer Biology 2020Quote: ... after washing sections were incubated with biotinylated secondary antibody at room temperature for 1 hour followed by incubation with ABC (Avidin Biotin complex) (Vector Labs) for 30 minutes ...
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bioRxiv - Microbiology 2021Quote: ... Slides were then washed with DI water and heated to 90C for 30 minutes in 1% citrate-based antigen unmasking solution (Vector Laboratories) using a Biocare Medical Decloaking Chamber ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibody signal of Cytokeratin 8 was amplified using biotinylated anti-rat antibody (1:300, 0.5 mg, FisherScientific Vector Laboratories, CAS#NC9016344), followed by staining with Streptavidin ...
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bioRxiv - Microbiology 2022Quote: ... for 15 min at room temperature and immunostained using the N protein 1C7C7 monoclonal antibody (1 μg/ml) and the Vectastain ABC kit (Vector Laboratories), following the manufacturers’ instruction ...
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bioRxiv - Neuroscience 2022Quote: ... primary antibody overnight at 4 °C and revealed with a biotinylated goat anti-rabbit IgG secondary antibody (1:1,000, 2 hat RT, Vector Laboratories, VECTBA1000), the VECTASTAIN ABC Elite HRP kit (Vector Laboratories ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed with PBS-T and then incubated for 2 h at RT with biotinylated secondary antibody (1:200, Vector Laboratories). After subsequent washes with PBS-T ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were rinsed with PBST and incubated with Cy3-conjugated donkey anti-rabbit IgG (1:500, dissolved in blocking solution; Vector Laboratories) secondary antibody for 2 h ...
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bioRxiv - Physiology 2022Quote: ... Tissues were rehydrated with 1X PBS for 5 minutes and blocked in 10% goat serum diluted in 1X PBS for 1 hour or MOM blocking reagent (Vector Labs). Primary antibodies (CD68 BioRad ...
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bioRxiv - Cell Biology 2022Quote: ... tissues were washed with PBS (20 min x 5) and incubated with the secondary biotinylated antibodies (1:1000 in PBS, Vector Labs) for 2 hrs at RT ...
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bioRxiv - Neuroscience 2023Quote: ... The brain slices were washed for three times in TBS and incubated in 10% normal goat serum in TBS-T with a biotinylated secondary antibody from goat against rabbit IgG (1:1000, Vector Laboratories) at room temperature for 2 hours ...