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Citations for Vector Labs :
1051 - 1100 of 1196 citations for GA binding protein alpha chain GABPA Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... slides were incubated with Alexa Fluor conjugated anti-mouse and anti-rat antibodies (1:200 dilution) and mounted using the Vectashield without DAPI (Vector Laboratories, H-1000). Images were acquired as described above ...
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bioRxiv - Neuroscience 2020Quote: ... The sections treated with biotinylated antibodies were further incubated for one hour in avidin-biotin-peroxidase solution (ABC Elite, Vector laboratories, Burlingame, USA). The protein was finally visualized by a 0.1M PB solution containing 5% 3,3’-diaminobenzidine (DAB ...
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bioRxiv - Microbiology 2021Quote: ... while sections labelled for CD3 were incubated with anti-mouse biotinylated secondary antibody (catalogue number BA-2000-1.5 Vector Laboratories, California, United States) at 1:50 with 1% BSA and thereafter incubated with Vectastain Elite ABC reagent (Vectastain Elite ABC Kit ...
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bioRxiv - Molecular Biology 2022Quote: ... Secondary antibody staining and biotin-streptavidin incubation were performed using species-specific VECTASTAIN Elite ABC kits (Vector Labs, PK-6101 and BMK-2202). DAB Peroxidase Substrate kit (Vector Labs ...
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bioRxiv - Developmental Biology 2022Quote: ... we first detected the DIG-labelled nkx2.4b riboprobe with anti-DIG antibody (1:6000) and BM purple staining in 28 hpf embryos and the DNP-labelled tg riboprobe with anti-DNP antibody (Vector Laboratories, 1:500) and NBT/BCIP (Roche ...
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bioRxiv - Cancer Biology 2022Quote: ... antibody (1 µg/mL, 3 h; Santa Cruz, #SC-816; secondary: biotinylated goat anti-rabbit IgG, 1:200, 16 minutes; Vector labs, #PK6101), or an anti-c-MYC antibody (1:100 ...
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bioRxiv - Immunology 2022Quote: ... sections were incubated using Alexa Fluor-conjugated secondary antibodies (Table S2) and counterstained using VECTASHIELD Antifade Mounting Medium with DAPI (H-1200, Vector Laboratories, Burlingame, CA). After drying for a few hours ...
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bioRxiv - Bioengineering 2022Quote: ... Secondary visualization of the primary antibodies was completed with ImmPRESS Horse anti-rabbit Ig Kit (Catalog No. MP-7401-50, Vector Laboratories, Burlingame, CA) or ImmPRESS Horse anti-mouse Ig Kit (Catalog No ...
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bioRxiv - Developmental Biology 2021Quote: ... Antibodies listed in Table 2 were detected using ImmPress HRP Universal antibody polymer detection kit (Horse Anti-mouse/rabbit IgG, Vector Labs, MP-7500), as previously described for mouse in50 ...
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bioRxiv - Neuroscience 2020Quote: ... tissue sections were rinsed and incubated at room temperature in a secondary biotinylated anti-mouse antibody for 4 hours (Vector Laboratories, Burlingame, CA). This series was then incubated with ABC solution and reacted with Vector SG (Vector Laboratories ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were washed four times in PBST and transferred to a secondary antibody solution of goat-anti-rabbit (Dylight Alexa flour 594, Vector Laboratories, Peterborough, UK) or at a dilution of 1:200 at PBST ...
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bioRxiv - Bioengineering 2020Quote: ... After the primary antibody, then treated with biotinlated donkey anti-rat IgG (LifeTech Thermo, A18743) followed by DAB staining using ABC kit (Vector Labs PK-6100) and DAB (Sigma D5637) ...
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bioRxiv - Neuroscience 2020Quote: ... sections were rinsed in PBS (5 × 5 min) and incubated in biotinylated anti-rabbit secondary antibody (1:1000 in PBS with 0.1% bovine serum albumin, Vector Laboratories, Inc., Burlingame, CA) for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: ... The sections were washed with PBS for 10 minutes 3 times and incubated with a biotinylated secondary antibody followed with avidin– HRP solution (Vectastain ABC HRP Kit; cat #: PK-4000; Vector Laboratories, Burlingame, CA) at 4°C overnight ...
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bioRxiv - Pathology 2020Quote: ... slides were rinsed in PBS and incubated in a horse anti-mouse/rabbit biotinylated IgG secondary antibody (VECTASTAIN Elite ABC Kit, Vector Labs, PK-6200). Sections were rinsed again ...
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bioRxiv - Neuroscience 2020Quote: ... in 2% BSA and 0.1 M TBS for 18 h at room temperature followed by incubation with biotinylated goat anti-rabbit Immunoglobin G secondary antibody (1:200; Vector Laboratories, Burlingame, CA) in 2% BSA and 0.1 M TBS for 75 min at room temperature ...
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bioRxiv - Pathology 2020Quote: ... The larvae were then washed in PBT and incubated in secondary antibody in PBT for 1hr followed by washing steps and mounted in VectaShield (Vector Laboratories, Burlingame, CA) on slides ...
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bioRxiv - Microbiology 2021Quote: ... was used for blocking the sections for 30 min at room temperature and incubated with Ki-67 antibody (#VP-RM04; Vector Laboratories, 1:100) overnight at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... washed 3 times with 0.1% PBST and then incubated for 1 hour at room temperature with ImmPRESS anti-rabbit peroxidase conjugated antibody (Vector Laboratories, Burlingame, CA, USA). Slides were again washed 3 times with 0.1% PBST with final wash in PBS prior to developing using freshly prepared DAB substrate (Vector Labs SK-4100) ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then washed in PBS and incubated with DyLight 594-conjugated goat anti-rabbit secondary antibody (1:1000, Vector Laboratories DI-1594) for 2 h on a shaker at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then washed in PBS and incubated with biotinylated goat anti-rabbit secondary antibody (1:600 dilution; Vector Laboratories, catalog #BA-1000) in PBS-Tx and 1% NGS for 2 h at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated with the appropriate secondary antibody conjugated to a fluorochrome and mounted with Vectashield® Vibrance™ Antifade Mounting medium with DAPI (Vector Laboratories) for nuclei counterstaining ...
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bioRxiv - Neuroscience 2020Quote: ... brain sections were incubated first with biotinylated goat anti-rabbit secondary antibody and then with avidin-biotin complex using the Elite Vectastain kit (Vector Laboratories, PK-6101). Chromogen reactions were performed with diaminobenzidine (SIGMAFAST DAB ...
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bioRxiv - Pathology 2023Quote: ... 20 Sections were processed following ImmPRESS HRP Universal Antibody (horse anti-mouse/rabbit IgG) Polymer Detection Kit and ImmPACT DAB EqV Peroxidase (HRP) Substrate (Vector Laboratories, Burlingame, CA) protocol ...
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bioRxiv - Neuroscience 2023Quote: ... USA) followed by secondary biotinylated antibody incubation (1:200, goat anti-rabbit IgG, Cat. No. BA-1000; Vector Labs Inc., Burlingame, CA, USA). Controls for nonspecific binding consisted in omitting the primary antibody (S Fig 5) ...
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bioRxiv - Cancer Biology 2023Quote: ... and then incubated in anti-Mouse IgG or anti-Rabbit IgG secondary antibodies conjugated to Horse Radish Peroxidase (ImmPACT DAB, Vector Laboratories, Newark, CA) in a humid chamber for 30 min at 24L ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were incubated with anti-mouse Ig-fluorescein antibody (1:200) and mounted on glass slides using DAPI (Vectashield, Vector Laboratories, CA, USA). Images were acquired in 40X magnification using a fluorescence microscope BX53 (Olympus Corporation ...
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bioRxiv - Immunology 2023Quote: ... Cells were then incubated for 30 min at RT with secondary antibody fluorochrome-conjugated anti-Rabbit Dylight 488 (Vector Laboratories, Newark, CA, USA). Finally ...
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bioRxiv - Neuroscience 2022Quote: ... slides were washed in TBS + 0.001 % Triton (2 x 5 min) and incubated with an ImmPress HRP secondary antibody (Vector Laboratories, MP-7401-15) for 1 h ...
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bioRxiv - Neuroscience 2022Quote: The sections were rinsed in PB and to block non-specific antibody binding they were preincubated for 1 h at room temperature in a stock solution containing 3% normal serum of the species in which the secondary antibodies were raised (Vector Laboratories, Burlingame, CA) in PB with Triton X-100 (0.25%) ...
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bioRxiv - Cancer Biology 2022Quote: ... staining was 2% goat serumPrimary antibodies were left overnight in 4 degrees and followed by anti-rabbit secondary (Vector Laboratories Cat# BA-1000). Next ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Sections were then washed and incubated with secondary antibody for 1h at 37°C and developed with ImmPACT Nova RED HRP substrate (Vector Labs, SK-4805). Tissues were counterstained with hematoxylin ...
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bioRxiv - Developmental Biology 2024Quote: ... Biotin-conjugated anti-goat or anti-rabbit secondary antibodies were each applied at 1:500 dilution and incubated with Vector elite Reagent (Vector Laboratories, CA, USA) for 45 minutes ...
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bioRxiv - Genetics 2024Quote: ... the slides were left at room temperature for 1 hour and then incubated for 30 min with the anti-mouse or anti-rabbit ImmPRESS HRP (peroxidase) secondary antibodies (Vector laboratories, CA, USA). Between all steps (except after the blockage of unspecific staining ...
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bioRxiv - Molecular Biology 2024Quote: ... Sections were processed following ImmPRESS HRP Universal Antibody (horse anti-mouse/rabbit IgG) Polymer Detection Kit and ImmPACT DAB EqV Peroxidase (HRP) Substrate (Vector Laboratories, Burlingame, CA) protocol ...
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bioRxiv - Developmental Biology 2023Quote: ... Sections were washed and then incubated in secondary antibody (Alexa 488) for 1 h at room temperature and mounted using VECTASHIELD with DAPI (VECTOR Laboratories Ltd, UK) and coverslips.
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bioRxiv - Neuroscience 2023Quote: ... 0.3% Triton X-100 (24 hours, 37°C) followed by peroxidase-conjugated secondary antibody (1:200 biotinylated anti-rabbit Vector Labs #BA-1000) and incubated in Elite ABC solution ...
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bioRxiv - Neuroscience 2023Quote: ... a blocking serum is added from the same species from which the secondary antibody is derived (ImmPRESS TM Anti-Rabbit and Anti-Mouse Kit from Vector Labs, Burlington, USA). The blocking was done at room temperature for a minimum of 20 minutes.
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated for 2 h (at RT) with a secondary biotinylated antibody followed by an avidin-biotin-peroxidase complex (ABC; Vector Laboratories, Peterborough, UK) for 1 hour in PBS ...
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bioRxiv - Immunology 2023Quote: ... The secondary antibodies were washed off with above-mentioned washing buffer for 15 min and finally slides were mounted with DAPI (Vector Laboratories, Burlingame, USA). The stained cells were examined under a Zeiss LSM 780 fluorescence microscope (Carl Zeiss MicroImaging ...
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bioRxiv - Neuroscience 2023Quote: ... labeled with rabbit anti-ionized calcium-binding adapter molecule (Iba)-1 antibody (Wako Chemicals, Neuss, Germany) and VectaStain Elite ABC-HRP kit (Cat.-No.: PK-6100, Vector Laboratories, Burlingame, USA), and visualized with diaminobenzidine (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies with their respective concentrations were as follows Griffonia Simplicifolia Lectin I (GSL I) Isolectin B4 (1:25, Vector Laboratories, #FL-1201), mouse monoclonal anti-3G5 IgM (1:10 ...
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bioRxiv - Developmental Biology 2022Quote: ... Ki67 staining was performed using monoclonal rabbit anti-Ki67 antibody (1:100, Linaris, Dossenheim, Germany) and signals were amplified by the use of the Vectastain Elite ABC Kit (Vector Laboratories, Burlingame, CA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Excess antibody buffer was removed with PBSTx and followed by incubation in biotinylated goat anti-mouse secondary antibody (1:1000 Cat# BP-9200-50: Vector Laboratories, California USA) overnight at 4°C in PBSTx and 1% NGS ...
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bioRxiv - Microbiology 2022Quote: ... brain sections were stained with rabbit anti-SINV capsid antibody (1:5000 dilution) and the Vectastain Elite ABC HRP kit (Vector Laboratories, PK-6101) was used for signal detection ...
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bioRxiv - Neuroscience 2023Quote: ... The secondary antibody incubation was with horse anti-mouse IgG conjugated to biotin (1:100 in PBS; Vector Labs, catalog BA-2000-1.5) for 2 h at room temperature ...
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bioRxiv - Bioengineering 2022Quote: ... Retinas were then washed 3 times in PBS at RT and incubated with polyclonal rabbit LM opsin and S opsin antibody (1/300; Vector Laboratories, Peterborough, UK) for 48 hours at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... sections were incubated with biotinylated secondary antibody for 30 minutes followed by ABC kit (VECTASTAIN Elite ABC-HRP kit, Vector Laboratories, PK-6100) for 30 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... The sections were washed with TBS and incubated with VectaFluor™ Excel Amplified Anti-mouse IgG Dylight™ 488 secondary antibody (DK-2488, Vector Laboratories) for 30 minutes at room temperature ...
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Small Molecule Screen Identifies Pyrimethamine as an Inhibitor of NRF2-driven Esophageal HyperplasiabioRxiv - Cancer Biology 2022Quote: ... antigens were retrieved on the deparaffinized sections for detection with primary antibodies (Table S1) and further detected with a streptavidin-peroxidase reaction kit and DAB as a chromogen (ABC kit; Vector Labs, Burlingame, CA). To ensure the specificity of the primary antibody ...