Labshake search
Citations for Vector Labs :
1051 - 1100 of 1131 citations for ERK1 2 Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... free–floating brain sections were rinsed in 0.1 M PBS (2 × 15 min) followed by a 30 min blocking in 3 % normal horse serum (Vector Laboratories, Burlingame, CA) and 0.3 % Triton X-100 in 0.1 M PBS ...
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bioRxiv - Immunology 2021Quote: ... Slides were mounted and nuclei were stained with 4’,6-Diamidino-2-Phenylindole (DAPI) fluorescent mounting medium (VECTASHIELD®, Vector laboratories Inc). Images were taken using confocal laser scanning microscope LEICA TCS SP8 (LEICA microsystems ...
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bioRxiv - Neuroscience 2022Quote: ... and cover slipped with a mountant containing the nuclear stain 4’,6-diamidino-2-phenylinodole (DAPI) (Vectashield; Vector Lab, Burlingame, CA, USA). Slides were stored in the dark at −20°C until imaging.
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bioRxiv - Developmental Biology 2020Quote: ... A small aliquot of embryo pellet (2 μL) was taken right before freezing and mixed with 10 μL VECTASHIELD® Antifade Mounting Medium with DAPI (Vector laboratories) and immediately frozen in dry ice ...
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bioRxiv - Microbiology 2021Quote: ... incubated with avidin-biotin complex kit (ThermoFischer Scientific, 32020) for 2 hrs and visualized with a 3,3’-diaminobenzidine kit (Vectastain, Vector Labs, SK-4100). Sections were then counterstained with cresyl violet for Nissl labeling.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The slides were then coverslipped with antifade mounting medium containing 4′,6-diamidino-2-phenylindole (DAPI; H-1200, Vector Laboratories, Burlingame, CA). Images were captured using a Nikon C2+ laser scanning confocal head mounted on an Eclipse Ti2 inverted microscope (Nikon ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were mounted and coverslips were applied with Vectashield mounting medium containing DAPI (4’-6-diamidino-2-phenylinodole; Vector Laboratories, Burlingame, CA). Sections were visualized on a Zeiss AxioImager M2 microscope (Zeiss ...
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bioRxiv - Plant Biology 2022Quote: ... the slides were dehydrated in 70-90-100% ethanol series for 3 min each and counterstained with 10 µg/ml 4’,6-diamidino-2-phenylindoline (DAPI) in Vectashield antifade medium (Vector Laboratories, USA). For immunodetection of microtubules ...
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bioRxiv - Neuroscience 2023Quote: ... tissue was incubated in a secondary solution containing 1:2 ImmPRESS® (peroxidase) polymer horse anti-mouse IgG (rat absorbed) (Vector Laboratories) in TBS for 30 minutes ...
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bioRxiv - Immunology 2023Quote: ... we used the lectin from Datura stramonium at a staining concentration of 10 µg/ mL (Biotinylated DSL, Vector laboratories, B-1185-2). 2 × 105 cells per condition were collected and rinsed three times with PBS ...
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bioRxiv - Neuroscience 2023Quote: ... 30-80 MΩ) lowered vertically and filled with a solution of potassium acetate (0.5M) and Neurobiotin (2%, Vector Laboratories, Burlingame, CA, USA). The signal was amplified and low-pass filtered (10kHz ...
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bioRxiv - Microbiology 2024Quote: ... The parasitophorous vacuoles (PV) of the parasites of were then stained with 2 μg/mL fluorescein labelled Vicia Villosa lectin (VVL; Vector Laboratories #ZG0124) in 1% BSA/PBS for 1 hour at room temperature then nuclei stained with 2 μg/mL DAPI (Merk #096M-4014V ...
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bioRxiv - Neuroscience 2024Quote: ... free–floating brain sections were rinsed in 0.1 M PBS (2 × 15 minutes) followed by a 30-minute blocking in 10% normal goat serum (NGS; Vector Laboratories, Burlingame, CA) and 0.4% Triton X-100 in 0.1 M PBS ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Slices were then washed overnight at 4 °C in a biotinylated Wisteria Floribunda Agglutinin (WFA, 1:2000, Vector Laboratories, B-1355-2) followed by washing 3x in PBS for 5 minutes before 60-minute incubation with a Streptavidin-conjugated Dylight 488 secondary (1:200 ...
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bioRxiv - Neuroscience 2019Quote: ... After overnight incubation in primary antibody,the sections were rinsed in washing buffer and then incubated for 1 h in an anti-rabbit biotinylated antibody (Vector Laboratories, #ba-9400; 1:200) in washing buffer containing 2% normal goat serum ...
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bioRxiv - Microbiology 2020Quote: ... The detection of antibody binding was performed with anti-rabbit IgG HRP-conjugated polymer (Zytomed GmbH, Berlin, Germany) or anti-rat IgG HRP-conjugated polymer ImmPRESS™ (Vector Laboratories, Burlingame, CA, USA) and 3-amino-9-ethylcarbazole (AEC ...
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bioRxiv - Cancer Biology 2020Quote: ... Primary antibodies were incubated with sections overnight at 4°C and detected using Vectastain Elite ABC HRP Kits (Vector Laboratories: Peroxidase Rabbit IgG PK-6101) and DAB substrate (Vector Laboratories ...
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bioRxiv - Pathology 2022Quote: ... overnight at 4°C then rinsed with TBST prior to detection using an anti-rabbit avidin-biotin amplification kit (Vector Laboratories Cat# PK-6101, RRID:AB_2336820). Vector NovaRed ...
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bioRxiv - Microbiology 2022Quote: ... the secondary biotinylated goat anti-mouse or anti-rabbit antibody was applied for 30 minutes at room temperature (Vector Laboratories, Burlingame, CA, USA, 1:200). Slides were scanned using Hamamatsu S60 scanner ...
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bioRxiv - Neuroscience 2019Quote: ... sections were incubated for 1 hr at room temperature in a biotinylated solution (biotinylated goat anti-rabbit IgG; 1:200 dilution; Vector Laboratories; catalog no. BA-1000) before incubating in an avidin-biotin horseradish peroxidase solution for 2 hrs at room temperature (Vector Novoxastra Laboratories) ...
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bioRxiv - Microbiology 2022Quote: ... Slides were washed thrice with PBS to remove excess antibody and incubated with a rabbit anti-goat biotinylated IgG (Vector laboratories catalogue number BA-5000) for 10 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... The second primary was added to the sections overnight at 4°C then detected with a goat α-rabbit DyLight® 488 (DI1488, Vector Laboratories, 1:200) and mounted using VECTASHIELD® Antifade Mounting Medium with DAPI (H-1200-10 ...
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bioRxiv - Immunology 2023Quote: ... primary antibody in Leica Primary Antibody Diluent for 30 min was followed by application of a rabbit anti-rat secondary (Vector Laboratories, Catalog Number AI-4001) for 8 min ...
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bioRxiv - Microbiology 2019Quote: ... and holdfasts were labelled using 50 µl of fluorescent Alexa Fluor (AF488 or AF594) conjugated lectins (Molecular Probes or Vector Labs, Table 2) at a final concentration of 0.5 µg/ml ...
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bioRxiv - Neuroscience 2019Quote: ... TC neurons in 250 micron brain slices or RGCs in flat-mount retinas were loaded by passive diffusion with 2% neurobiotin (Vector Labs, Burlingame, CA) and/or 100 μM CF568 dye (Biotium ...
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bioRxiv - Genetics 2021Quote: ... the gonads were again washed three times with PBSTw and resuspended in 1 drop of Vectashield containing 4’,6-diamidino-2-phenylindole (DAPI) (Vector Laboratories, Burlingame, CA). The gonad was finally mounted on pads of 5% agarose in water on microscope slides and covered with a cover glass (Sulston and Horvitz ...
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bioRxiv - Cell Biology 2021Quote: ... pelleted (400 g, 6 minutes) and resuspended in 2 to 3 drops (∼75 μL) of Vectashield Antifade Mounting Medium (Vector Laboratories, H-1000). This suspension was then mixed thoroughly by pipetting to separate clumped cells ...
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bioRxiv - Neuroscience 2021Quote: ... a thin slice of dorsal spinal cord (∼2 mm thick) with attached DR stumps was cut with a microscissors and mounted on slides in Vectashield (Vector Laboratories, Burlingame, CA).
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bioRxiv - Neuroscience 2021Quote: ... The slices were then washed three times with PBS and mounted with 4’,6-diamidino-2-phenylindole (DAPI)-containing Vectashield (Vector Laboratories, CA, USA). Cell counting was done manually on brain sections containing the mPFC ...
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bioRxiv - Molecular Biology 2019Quote: ... DAB staining was developed under a light microscope for between 2-10 min as per the manufacturer’s instructions with the ImmPACT DAB peroxidase substrate kit (Vector Labs, Cat# SK-4105). Slides were then counterstained with Hematoxylin Solution ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated in avidin-biotinylated horseradish peroxidase complex (6 μl/ml avidin and 6 μl/ml biotin; 2 hours at room temperature; Vector Laboratories, CA, USA), washed in PB ...
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bioRxiv - Developmental Biology 2021Quote: ... embryos were incubated in the second antibody solution for 1 h at 37° C and subsequently mounted in Vectashield mounting medium containing 4′,6-diamidin-2-phenylindol (DAPI, Vector Laboratories, Burlingame, California) in a manner that conserves the 3D structure of the specimen [42] ...
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bioRxiv - Microbiology 2020Quote: ... Cells were counterstained with VECTASHIELD® Antifade Mounting Medium containing 1.5 µg ml- 1 4,6-diamidino-2-phenylindole (Vector Laboratories, Burlingame, CA, USA). Images were captured on a BX61 microscope equipped with a DP80 camera (Olympus Australia ...
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bioRxiv - Bioengineering 2022Quote: ... Secondary antibodies were incubated with the sections for one hour before washing using PBS and mounting using Vectashield Antifade Mounting Medium with 4’,6-diamidino-2-phenylindole (DAPI) (Vector Laboratories, Burlingame, CA).
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bioRxiv - Neuroscience 2022Quote: ... Cells incubated with MitoSOX™ were then fixed with 3.7% paraformaldehyde and applied with an anti-fading mounting medium with 4′,6-diamidino-2-phenylindole (DAPI; H-1200, Vector Laboratories, Burlingame, CA). Live or fixed cell images were captured at an excitation/emission (in nm ...
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bioRxiv - Neuroscience 2023Quote: ... slices were incubated overnight at 4°C with a solution containing biotinylated Wisteria floribunda Lectin (WFA, B-1355-2, Vector Laboratories, 1:200) and 3% BSA in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... The sections were mounted onto gelatinized slides and left to dry (at least 2 hours) before being coverslipped with VectaShield (Vector Laboratories, Burlingame, CA). The sections were imaged with 10x objective and 10x eyepiece magnification on a Leica LSI 3000 microscope ...
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bioRxiv - Neuroscience 2022Quote: ... slides were washed in TBS + 0.001 % Triton (2 x 5 min) and incubated with an ImmPress HRP secondary antibody (Vector Laboratories, MP-7401-15) for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... for 40 seconds and sections were finally coverslipped with Vectashield mounting medium containing 4′,6-diamidino-2-phenylindole (DAPI) to label the nucleus (Vector Laboratories, H-1800). Slides were kept at 4 °C until imaging ...
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bioRxiv - Neuroscience 2023Quote: ... tissue was washed with 0.1 M TBS (5 × 5) and the stain was allowed to develop for 2 minutes using an ImmPACT novaRED Substrate Kit (Vector Laboratories, SK-4805). Tissue sections were then washed for 5 min in tap water ...
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bioRxiv - Cancer Biology 2022Quote: ... The sections were washed with PBS and mounted using VECTASHIELD® Vibrance™ mounting medium with 4′,6-diamidino-2-phenylindole (DAPI, Vector Laboratories). Table S6 lists the primary and secondary antibody dilutions for the organ tissues ...
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bioRxiv - Physiology 2023Quote: ... the sections were mounted with hard-set DAPI (4′, 6-diamidino-2-phenylindole –hard set; Cat# H-1500, Vector Laboratories Ltd., Peterborough, UK). The slides were left overnight in the dark at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then washed 2 × 30s in 1M tris-HCl solution and then incubated in a DAB reaction solution (Vector Labs, SK-4100) for 5-15 min ...
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bioRxiv - Evolutionary Biology 2023Quote: ... pH 7.4 containing 0.1% Tween 20) and squashed on a glass slide with Vectashield mounting medium containing 4,6-diamidino-2-phenylindole (DAPI; Vector Laboratories, Newark, California, USA). Observations were performed using an Olympus BX61 microscope (N = 45-193 individuals per strain).
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bioRxiv - Physiology 2023Quote: ... The coverslips were washed in PBS before being mounted onto a microscope slide with 4’,6-diamidino-2-phenylindole (DAPI) (Vectashield, Vector Laboratories, Burlingame, CA) mounting medium.
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bioRxiv - Neuroscience 2023Quote: The procedure was similar to that of single-cell recordings except that the micropipette was filled with 2% Neurobiotin (SP-1120, Vector Laboratories, CA., USA) in 0.5 M NaCl ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated for 2 h (at RT) with a secondary biotinylated antibody followed by an avidin-biotin-peroxidase complex (ABC; Vector Laboratories, Peterborough, UK) for 1 hour in PBS ...
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Targeting MOG to skin macrophages prevents EAE in macaques through TGFβ-induced peripheral tolerancebioRxiv - Immunology 2019Quote: ... Incubation with primary antibodies was followed by a biotinylated goat-anti-rabbit antibody for 30 min followed by the avidin–biotin–peroxidase complex (Vectastain Elite ABC Kit, Vector Laboratories, PK 6100; Burlingame, CA, USA) for 30 min at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... sections were washed in PBST and incubated for 60 minutes at room temperature with a secondary biotinylated goat anti-rabbit antibody (Vector laboratories, Cat# BA-1000, lot# 2F0430) diluted 1:1000 ...
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bioRxiv - Molecular Biology 2023Quote: ... slides were incubated for 1 hour with a secondary antibody cocktail: 1:250 anti-rabbit IgG Texas Red 594 (Vector Labs, Newark, CA, USA; TI-1000) + anti-mouse IgG2b AF488 (Thermo Fisher Scientific ...