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Citations for Vector Labs :
951 - 1000 of 1186 citations for E3 Ubiquitin Protein Ligase DTX1 DTX1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated with secondary antibodies (anti-rabbit, 1:400; DI-1594; anti-mouse, 1:1000; fl-2000; Vector laboratories, USA) for 2 h ...
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bioRxiv - Cell Biology 2022Quote: ... tissues were washed with PBS (20 min x 5) and incubated with the secondary biotinylated antibodies (1:1000 in PBS, Vector Labs) for 2 hrs at RT ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated with a biotin-conjugated anti-mouse secondary antibody (1:200) and revealed with the DAB peroxidase substrate kit (Vector laboratories) before imaging with a Nikon Eclipse 80i microscope ...
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bioRxiv - Physiology 2024Quote: ... was incubated at 4°C overnight and a biotinylated secondary antibody followed by streptavidin-DyLight 594 (BA1000 and SA-5594, Vector Laboratories) were used for cardiomyocyte identification ...
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bioRxiv - Cancer Biology 2023Quote: ... slides were incubated for 30 minutes with the appropriate secondary antibody: ImmPRESS HRP Anti-Rabbit IgG secondary (Vector Laboratories, MP-7401). Immunodetection was performed using the HRP system and visualized with chromogen 3,3’-diaminobenzidine (Vector Laboratories ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were washed and incubated first with a Biotinylated Goat Anti-Rabbit IgG Antibody (over-night at 4°C-Vector Laboratories), and then with Alexa Fluor™ 594 streptavidin (3 hours – room temperature-Thermo Fisher) ...
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bioRxiv - Immunology 2024Quote: ... Successful attachment of azide groups to antibodies was confirmed by a test conjugation with DBCO-AZDye (Click Chemistry Tools/Vector Laboratories) followed by detection of fluor-conjugated antibodies using UltraComp eBeads (ThermoFisher) ...
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bioRxiv - Immunology 2024Quote: ... We then used flow cytometry to confirm higher expression of Tn on the AT3 cell surfaces using the ReBaG6 antibody (generously provided by Richard Cummings at Harvard University) (Supplementary Fig. 1A) (57) and VVL lectin (Vector Laboratories) (Supplementary Fig ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by a 10 min incubation with secondary antibody (either ImmPress-mouse HRP, MP-7402, or ImmPress-rabbit HRP, MP-7401, Vector Laboratories) and fluorescent labelling for 10 min using tyramide signal amplification with Opal dyes in the sequence of 690 ...
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bioRxiv - Cancer Biology 2024Quote: ... Incubation with biotinylated antibodies slides followed by modified Elite avidin-biotin peroxidase (ABC) method (Vector Laboratories, Burlingame, CA, USA; pk-6100) for 30 minutes (RT) ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were washed with DPBS and incubated with secondary antibody (Jackson) 1:100 in DPBS for 1 hour and mounted with DAPI (Vector Labs).
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bioRxiv - Biochemistry 2024Quote: ... after washing with PBS the bone sections were treated for 1 h with biotinylated goat anti-rabbit IgG secondary antibody (1:200; Vector Laboratories). The sections were developed using the ABC system (Vector Laboratories ...
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bioRxiv - Neuroscience 2024Quote: ... It was reacted with a second antibody and the positive brown signal generated with DAB peroxidase-based methodology (Vector Laboratories, SK4100). Sections were counterstained with haematoxylin alone ...
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bioRxiv - Neuroscience 2023Quote: ... the neuronal cultures were washed with PBS three times and incubated in Alexa Fluor conjugated secondary antibodies and DAPI (Vector Labs) diluted in a blocking buffer containing 10% NGS for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... The brain slices were washed for three times in TBS and incubated in 10% normal goat serum in TBS-T with a biotinylated secondary antibody from goat against rabbit IgG (1:1000, Vector Laboratories) at room temperature for 2 hours ...
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bioRxiv - Neuroscience 2023Quote: ... The next day sections were washed twice with TBST and incubated with biotinylated anti-rabbit or anti-mouse antibodies (Vector Laboratories) diluted 1:200 in blocking buffer for 1h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... using Dako Link48 plus as a service with the primary antibodies described in the Key Resources Table and species-appropriate ImmPRESS Polymer Detection Kit (Vector Laboratories) and developed with 3,3’-Diaminobenzidine (DAB ...
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bioRxiv - Neuroscience 2023Quote: ... sections were washed in PBS-T and incubated in blocking solution containing biotinylated goat anti-rabbit secondary antibody (1:250, Vector Laboratories) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... sections were washed in PBS and incubated for two hours in goat-anti-rabbit secondary antibody (Vector Laboratories, Burlingame, CA, USA) at a 1:200 concentration ...
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bioRxiv - Molecular Biology 2023Quote: ... vWAT sections were incubated overnight at 4°C with primary antibody (CD68, 1:100 in PBST) and visualised by probing with Horse Anti-Rabbit IgG Polymer Kit (MP-7401, Vector Labs) for 1 hour at 37°C and ImmPACT DAB Peroxidase Substrate Kit (SK-4105 ...
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bioRxiv - Cancer Biology 2023Quote: ... This procedure was modified for mouse primary antibodies for use with the Mouse on Mouse Immunodetection Kit (BMK-2202; Vector Laboratories) according to manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... free-floating sections were rinsed and bound antibodies were visualized by the Avidin-Biotin complex (ABC) amplification system (Vectastain ABC kit, Vector Laboratories) using 3,3′-diaminobenzidine tetrahydrochloride (DAB Substrate Kit ...
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bioRxiv - Neuroscience 2023Quote: ... sections were washed in 0.2% Triton X-100 PBS and incubated in secondary antibody solution for 1 hour before amplification of the signal by incubation in Vectastain ABC kit (1:400, Vector Laboratories) for 1 hour ...
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bioRxiv - Molecular Biology 2023Quote: ... This was followed by incubation with HRP conjugated 2° antibodies (α-mouse (PI-2000) and α-rabbit (PI-1000, Vector Labs)) in 5% Milk in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... Incubation with the biotinylated secondary antibody (1:150, Vector in 5% NGS in PBST) for 1 h was followed by the Vector ABC Kit (Vector Laboratories, solution A and solution B in PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... Development of the biotinilated antibody signal was done by 1 h incubation with the Avidin/Biotin-HRP complex (Elite Vectastain ABComplex Kit, Vector Laboratories) following 1 h incubation with Streptavidin-647 (BioLegend) ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were washed with PBS and blocked and stained using appropriate primary antibodies and reagents from the Vectastain Elite ABC kit (Vector Labs). Diaminobenzidine exposure was titrated to optimal contrast ...
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bioRxiv - Cell Biology 2023Quote: ... Sections and myofibers that were stained with anti-Pax7 antibody (DSHB) also included a block with 1:50 Mouse on Mouse Ig Blocking Reagent (MKB-2213, Vector Labs).
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bioRxiv - Cancer Biology 2023Quote: ... Chromogen amplification and detection of antibodies was performed using the Vectastain Elite ABC-HRP Kit and DAB Peroxidase Substrate Kit (Vector Labs). Antigen unmasking was performed with a citrate buffer pH 6.0 by a modified microwave retrieval method or boiling ...
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bioRxiv - Physiology 2023Quote: ... of paraffin-embedded tissues using specific commercially available primary antibodies (Supplementary Table S1) and AlexaFluor 488 and 568-conjugated F(ab’) secondary antibody (Molecular Probe) or VECTASTAIN ABC kit (Vector lab). Cell-specific CD68 positive and total cell numbers were counted in the area of 0.07244 mm2 ...
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bioRxiv - Neuroscience 2023Quote: ... BA-2000)/ goat anti-guinea pig (BA-7000)/ goat anti-rabbit (BA-1000) or horse anti-goat (BA-9500) biotinylated secondary antibodies overnight (1:500, Vector Laboratories) followed by extensive washes in TBS (3×10 min ...
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bioRxiv - Neuroscience 2023Quote: ... followed by incubation with a biotin-conjugated anti-rabbit or anti-mouse antibody (1:250, Vectastain Elite ABC kit, Vector laboratories). Sections were counterstained with Nissl (0.09% thionin ...
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bioRxiv - Microbiology 2023Quote: ... the secondary antibody was applied for 30 min and rinsed (twice) in TBST before reaction with Vector ImmPACT DAB (Vector Labs) for 5 min ...
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bioRxiv - Cell Biology 2023Quote: ... The blots were washed in TBST 3X followed by incubation in blocking buffer containing a goat anti-rabbit HRP tagged secondary antibody (Vector Labs) for 2 hr RT ...
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bioRxiv - Cancer Biology 2023Quote: ... slides were incubated with Alexa Flour-conjugated secondary antibody for 1 hr at room temperature and mounted in Vectashield mounting medium containing DAPI (Vector Laboratories). Nikon A1 Confocal microscope (Nikon ...
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bioRxiv - Neuroscience 2023Quote: ... The slices were washed with PBS (10 min, three times) and incubated with biotin-labeled goat anti-rabbit secondary antibody (1:500; Vector Labs) at room temperature for 90 min ...
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bioRxiv - Cancer Biology 2024Quote: ... samples were incubated for 90 minutes with appropriate secondary pre-diluted antibody from the Vectastain ABC kit (Vector Laboratories Ltd, UK).
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bioRxiv - Neuroscience 2024Quote: Sections were then incubated in secondary antibody (biotinylated anti-goat IgG made in horse, 1:500, Vector Laboratories, see Table 2) for 1 hr in Tris B ...
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bioRxiv - Cancer Biology 2024Quote: ... the cells were incubated with 594 (or 488) labeled secondary antibodies (1:200) and DAPI-containing mounting solution VECTASHIELD (Vector Laboratories). The slices were visualized by using a Nikon inverted microscope Eclipse Ti-U equipped with a digital camera ...
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bioRxiv - Cancer Biology 2024Quote: ... membranes were washed in 1X TBST 5 times and incubated with horseradish peroxidase (HRP)-conjugated secondary antibody (1:1000 in 1X TBST) (Vector Laboratories) for 1 hour ...
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bioRxiv - Neuroscience 2024Quote: ... was followed by incubation with the primary antibody (Table S2) at 4°C for 24 or 48h in 2% (vol/vol) serum and with the corresponding biotinylated antibody (Vector Laboratories) or alkaline-phosphatase antibody (ab6722 ...
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bioRxiv - Molecular Biology 2024Quote: ... Slides were then washed for 5 minutes in PBS and incubated in a secondary antibody solution (1x PBS and 1% [or 1:100 dilution] Texas Red-conjugated anti-rabbit IgG [catalog #: TI-1000; Vector Laboratories, Burlingame ...
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bioRxiv - Immunology 2024Quote: ... was diluted 1:500 and applied for 30 min at RT followed by biotinylated secondary rabbit anti-goat antibody 1:400 (BA-5000; Vector Laboratories) for 10 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... On the following day sections were incubated in biotinylated goat anti-rabbit secondary antibody (1:500, #BA-1000, Vector laboratories, RRID: AB_2313606) and avidin-biotin complex (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... signal amplification after species-specific secondary biotinylated antibody incubation of two hours was done using an avidin/biotin-based peroxidase system (PK6100, ABC Vectastain Elite kit, Vector Laboratories, UK). Chromogen development was sequential with double labelling ...
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bioRxiv - Neuroscience 2021Quote: ... re-blocked and probed with the relevant primary and secondary antibodies (bound to HRP) then developed with DAB + Nickel (SK4100, Vector Laboratories, UK). Chromogen development reactions were halted with distilled water ...
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bioRxiv - Neuroscience 2021Quote: ... blocked with 3% NDS-PB-TX and first incubated in biotinylated secondary anti-mouse antibody (1:200, Vector Labs, CAMK-IIα amplification) overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... The tissue was rinsed in TBS-X and reacted with the respective biotinylated secondary antibody (1:200, Vector Laboratories Ltd., Peterborough, UK) for 60 min at room temperature in 1% BSA in TBS-X ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were incubated at 4 °C overnight with primary antibody diluted in PBS containing 0.3% Triton X-100 and 5% normal goat serum blocking solution (Vector Laboratories, #S-1000). Antibodies were as follows ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were then incubated with secondary anti-mouse antibody (Immpress-HRP anti-mouse IgG (peroxidase polymer detection kit, Vector Laboratories, Burlingame, CA) for 30 min ...