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Citations for Vector Labs :
901 - 950 of 2268 citations for 3 1 Pyrrolidinylmethyl phenyl magnesium bromide solution since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... Heat-induced epitope retrieval was done using a pressure cooker containing 15 ml of Antigen Citrate-based unmasking solution (Vector laboratories; H-3300), diluted in 1.6 L of dH2O ...
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bioRxiv - Neuroscience 2024Quote: ... Three 15-minute rinses in PBS were followed by a one hour room temperature incubation in an avidin-biotin peroxidase PBS solution (Vector Laboratories, Burlingame, CA). Finally ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were rinsed once in TX-PBS and twice in PBS-/-before color development for 10 min in ImmPACT Vector SG Substrate chromogen solution (Vector Laboratories, #AK-4705) without H2O2 ...
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bioRxiv - Microbiology 2024Quote: ... The wells were then incubated in a primary antibody blocking solution containing rabbit anti-GAP45 or guinea pig anti-CDPK1 or biotinylated dolichos (Vector labs B-1035) for 60 minutes at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... sections were deparaffinized in xylene followed by heat-induced antigen retrieval using Citrate-based Antigen Unmasking Solution at pH 7.0 (Vector Labs, Newark, CA, USA). Sections were blocked in 10% normal goat serum ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed 3x in 0.1M PBS for 10 min per wash and horseradish peroxidase was visualized using 3,3’ diaminobenzidine (DAB) in a 3 M sodium acetate buffer containing 2.5% nickel sulfate and 0.05% H2O2 (SK-4100, Vector Laboratories, Inc, Burlingame, CA) for 3 min ...
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bioRxiv - Cancer Biology 2022Quote: ... in phosphate buffer 0.2 % Triton supplemented with10 % horse serum and rinsed 3 more times 10 min before being mounted in Vectashield (Vector Labs, H-1000). For Phalloidin staining ...
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bioRxiv - Neuroscience 2021Quote: ... After inactivation of endogenous peroxidases and blocking with 3% H2O2 the tissue was treated with Avidin/Biotin Blocking Kit (SP-2002 Vector Labs, USA) followed by an incubation overnight at 4°C with antiDsRed rabbit polyclonal primary antibody (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... free–floating brain sections were rinsed in 0.1 M PBS (2 × 15 min) followed by a 30 min blocking in 3 % normal horse serum (Vector Laboratories, Burlingame, CA) and 0.3 % Triton X-100 in 0.1 M PBS ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then washed 3 times for 5 minutes with PBS and mounted using VECTASHIELD Antifade Mounting Medium (Vector Laboratories, H-1000) or Prolong Gold Antifade Mountant with DAPI (Thermo Fisher ...
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bioRxiv - Cell Biology 2021Quote: ... Slides were again washed 3 times with 0.1% PBST with final wash in PBS prior to developing using freshly prepared DAB substrate (Vector Labs SK-4100). Slides were rinsed with water and counterstained with Hematoxylin (RICCA Chemical Company ...
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bioRxiv - Cancer Biology 2021Quote: ... and cuticles were washed 3 times in PBSS before mounting on glass slides in Vectashield mounting media without DAPI (Vector Laboratories, Inc.). Tumours were stained overnight at 4°C with DAPI ...
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bioRxiv - Plant Biology 2022Quote: ... the slides were dehydrated in 70-90-100% ethanol series for 3 min each and counterstained with 10 µg/ml 4’,6-diamidino-2-phenylindoline (DAPI) in Vectashield antifade medium (Vector Laboratories, USA). For immunodetection of microtubules ...
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bioRxiv - Neuroscience 2022Quote: ... free-floating sections were washed with PBS 3 times and blocked in 5% normal goat serum (NGS, Vector Laboratories, S-1000-20) and 0.1% Triton X-100 (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... slices were washed for 3 times in 0.1 M PBS and mounted on glass slides using Vectashield medium containing DAPI (Vector Labs, Peterborough, UK).
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bioRxiv - Neuroscience 2024Quote: Free-floating brain sections were first washed with a series of PBS and 0.5% PBS-Triton X-100 (PBS-T) before applying a 3% normal goat serum (NGS, #1000, Vector Laboratories, Burlingame, CA) blocking solution for 1 hour at room temperature (RT) ...
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bioRxiv - Genetics 2024Quote: ... Slides were washed for 5 minutes 3 times and mounted in Vectashield plus DAPI (4’,6-diamidino-2-phenylindole) (Vector Laboratories, USA). Stained testis spreads were imaged using an Olympus delta vision or Zeiss Observer microscope using 40X or 63X objectives.
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bioRxiv - Pathology 2024Quote: ... or anti-mouse (1:2,500, PI-2000-1, Vector Labs) secondary antibodies ...
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bioRxiv - Cell Biology 2024Quote: ... 1:40 Mouse on Mouse (Vector Laboratories; MKB-2213-1), syringe filtered ...
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bioRxiv - Neuroscience 2020Quote: ... Non-specific binding of secondary antibodies was minimised by incubating the tissue sections in a TBS-Tx solution containing 20% normal serum from the species of which the secondary antibodies were raised (Vector Laboratories, Burlingame, CA, USA), for one hour at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Non-specific binding of secondary antibodies was minimised by incubating the tissue sections in a TBS-Tx solution containing 20% normal serum from the species of which the secondary antibodies were raised (Vector Laboratories, Burlingame, CA, USA), for two hours at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated at room temperature for 1.5 hours in secondary antibody solution containing either biotinylated rabbit anti-goat IgG (Vector Laboratories, Burlingame, CA; PK-6105) or biotinylated goat anti-rabbit IgG (Vector Laboratories ...
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bioRxiv - Neuroscience 2023Quote: ... free-floating sections were first washed 3 × 10 min in 1x PBS and then incubated for 30 min in a blocking solution of 2% normal goat serum (S-1000-20; Vector Laboratories, Newark, CA, USA) and 0.1% Triton x-100 (T8787-50ML ...
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bioRxiv - Cell Biology 2023Quote: ... sections were subjected to heat-induced epitope retrieval pre-treatment at 120 °C using citrate-based antigen unmasking solution (Vector Laboratories, Burlingame, CA, USA), tris-based antigen unmasking solution (Vector Laboratories) ...
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bioRxiv - Cancer Biology 2023Quote: ... sections were subjected to heat-induced epitope retrieval pre-treatment at 120 °C using citrate-based antigen unmasking solution (Vector Laboratories, Burlingame, CA, USA). For immunofluorescence ...
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bioRxiv - Cancer Biology 2023Quote: ... sections were subjected to heat-induced epitope retrieval pre-treatment at 120 °C using citrate-based antigen unmasking solution (Vector Laboratories, Burlingame, CA, USA). For immunofluorescence ...
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bioRxiv - Bioengineering 2023Quote: ... cells were washed three times with PBS and mounted onto a glass slide with VectaShield DAPI mounting solution (Vector Labs, Cat # H-1800-10). For each sample ...
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bioRxiv - Cell Biology 2023Quote: ... DAPI was used to stain the nuclei and samples were mounted with VectaShield mounting solution (H-1200; Vector Laboratories, Inc, Newark, CA, United States). Confocal microscopy was performed on a Leica SP8 images were analyzed with the Leica Application Suite X (Leica Microsystems Inc. ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were then incubated in deionized water for one minute prior and transferred to the BioGenex EZ-Retriever System where they were incubated in antigen unmasking solution (Vector Laboratories; Cat# H-3300) and microwaved for 15 minutes at 95°C ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were then incubated in deionized water for one minute prior and transferred to the BioGenex EZ-Retriever System where they were incubated in antigen unmasking solution (Vector Laboratories; Cat# H-3300) and microwaved for 15 minutes at 95°C ...
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bioRxiv - Neuroscience 2024Quote: ... Slides were washed in deionized water for 1 min prior to transfer to the BioGenex EZ-Retriever System where they were incubated in an antigen unmasking solution (Vector Laboratories; Cat# H-3300) at 95°C for 15 min ...
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bioRxiv - Neuroscience 2024Quote: ... The slices were mounted on glass slides with a mounting solution containing DAPI (4’,6-diamidino-2-phenylindole; Vectashield with DAPI, Vector Laboratories, Burlingame, CA, USA). Images were acquired using a slide scanning system (Panoramic Scan ...
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bioRxiv - Neuroscience 2024Quote: ... Slides were then incubated in deionized water for one minute prior to transfer to the BioGenex EZ-Retriever System where they were incubated in antigen unmasking solution (Vector Laboratories; Cat# H-3300) and microwaved for 15 minutes at 95°C ...
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bioRxiv - Cell Biology 2024Quote: ... hydrogen peroxide in methanol to block endogenous peroxidase and then incubated with Normal Goat Serum Blocking Solution for rabbit antibody (S-1000, Vector Laboratories, Newark, CA, USA) to block nonspecific sites ...
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bioRxiv - Cell Biology 2020Quote: ... The coverslips with cells were mounted on 3 cm glass bottom cell culture plates using Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA). Random fields on the coverslips were photographed using an inverted Olympus IX81 microscope (Olympus Europe Holding Gmbh ...
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bioRxiv - Cell Biology 2020Quote: ... Sections were incubated at room temperature with 3% H2O2 for 10 minutes to quench endogenous peroxidase activity and blocked by 5% normal goat serum (Vector Laboratories# S-1000) in TBST (TBS containing 0.1% Tween® 20 ...
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bioRxiv - Neuroscience 2020Quote: ... Endogenous peroxidase activity and non-specific binding were blocked by incubation with 3% hydrogen peroxide in methanol for 15 minutes followed by 5% normal goat serum (Vector Laboratories, CA, USA) with 0.1% TWEEN-20 in PBS for 1 hour ...
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bioRxiv - Neuroscience 2020Quote: ... Brain sections that contained the ventrolateral preoptic nucleus were collected and blocked in PBS containing 0.25% Triton X-100 and 3% normal donkey serum (Vector Laboratories, Burlingame, CA, USA) for 60 min at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... pelleted (400 g, 6 minutes) and resuspended in 2 to 3 drops (∼75 μL) of Vectashield Antifade Mounting Medium (Vector Laboratories, H-1000). This suspension was then mixed thoroughly by pipetting to separate clumped cells ...
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bioRxiv - Pathology 2020Quote: ... cryoslides were washed 3 times in PBS and then slides were fluoromounted using Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA, USA).
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bioRxiv - Cell Biology 2020Quote: ... The signal was revealed using Vectastain Elite ABC-HRP kit and AEC Peroxidase (HRP) substrate 3-amino-9ethylcarbazole (VECTOR AEC Peroxidase Subtract Kit SK-4200, Vector Laboratories, Burlingame, CA). Samples were mounted using ProLong Gold antifade reagent (Life Technologies ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then washed 3 times with PBS and mounted on slides with VECTASHIELD® Vibrance™ Antifade Mounting Medium with DAPI (Vector laboratories).
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bioRxiv - Molecular Biology 2023Quote: ... In the end slides were washed 3 times with 0.1%Tween in PBS and mounted with VECTASHIELD Antifade Mounting Medium with DAPI (Vector Laboratories, H-1200-10). The images were acquired using Zeiss LSM800 confocal microscope and analyses using Fiji software 86 and visualized and deposited using OMERO 87 (project ID:12920).
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bioRxiv - Cell Biology 2023Quote: ... slides were washed in 3 x 5 min in TBST and mounted with Vectashield® mounting medium (Cat. No. H-1000; Vector Laboratories, USA).
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bioRxiv - Neuroscience 2024Quote: ... for 30 seconds to block endogenous fluorescence signal before washing in PBS (3 x 2 mins) and cover slipped and mounting with Vectashield antifade mounting medium with DAPI (H1900, Vector Laboratories, Peterborough, UK).
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bioRxiv - Neuroscience 2024Quote: ... slides were washed with PBS and incubated for at least an hour with blocking buffer containing 3% normal goat serum (NGS; Vector Laboratories, Burlingame, CA) and 0.4% Triton X-100 (Sigma Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... The proximal end of the femur was first dipped in phosphate-buffered saline with 0.5% Triton-X (PBST) for 2-3 seconds and immediately transferred to a Vaseline® well of neurobiotin 488 tracer (Vector Labs, USA) solution prepared in 1M NaCl ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were washed 3 times with PBST for 5 min each and mounted with Vectashield vibrance with DAPI (Cat. # H-1800-10, Vector Labs, Newark, CA). Cells were imaged at 63x on the confocal 60 μm disk setting with the BioTek Cytation C10 using the Gen5 software (Agilent) ...
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bioRxiv - Neuroscience 2022Quote: ... and UEA-1 594 (1:1000; Vector Laboratories, Cat# DL-1067), in antibody diluent (Dako ...
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bioRxiv - Cancer Biology 2022Quote: ... and Tomato Lectin Biotinylated (1:250, Vector Laboratories, B-1175-1) for one hour at room temperature followed by APC-Streptavidin secondary (1:500 ...