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Citations for Vector Labs :
901 - 950 of 1062 citations for 2 Amino 3 chloro 5 nitro 6 picoline since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Slides were blocked/permeabilized for 1 hour at room temperature with a solution of 5% Normal Horse Serum (Vector Laboratories, Burlingame, California), 0.2% Triton X-100 (Amresco ...
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bioRxiv - Neuroscience 2022Quote: ... or Phaseolus vulgaris leucoagglutinin (PHA-L; 2.5% solution in 10 mM phosphate buffer, pH 7.4; Vector Laboratories, Cat. No. L-1110-5). To reduce the number of animals ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were washed 4 × 10min in TBS 0.1% Tween and incubated with one of the following peroxidase-conjugated secondary antibodies diluted in TBS 0.1% Tween supplemented with 5%BSA for 2h at room temperature: horse anti-mouse IgG(H+L) (Vector laboratories, PI-2000), goat anti-rabbit IgG(H+L ...
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bioRxiv - Neuroscience 2023Quote: ... slides were deparaffinized and boiled for 20 min in citrate buffer (0.1M sodium citrate and 0.1M citric acid in distilled H2O) for antigen retrieval and incubated with 5% normal rabbit serum (Vector laboratory #S-5000) in PBS containing 0.2% Triton for 1 h at RT to block unspecific binding ...
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bioRxiv - Cancer Biology 2024Quote: ... Sections were washed with 1x PBS for 5 min and antigen retrieval was performed with Antigen Unmasking Solution Citrate-based pH 6.0 (Vector Laboratories H-3300) in a Decloaking Chamber™ (Biocare Medical ...
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bioRxiv - Cell Biology 2024Quote: ... After blocking with 5% goat serum/1% bovine serum albumin/PBS and Mouse on Mouse blocking reagent (Vector Laboratories, Newark, CA, USA), the samples were incubated with primary antibodies overnight at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: Immunohistochemistry on 5 µm sections from frozen or paralin-embedded tissues was performed using the biotin-peroxidase method (Vector laboratories, Burlingame, CA). IHC staining and scoring were evaluated by a trained pathologist.
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bioRxiv - Physiology 2024Quote: ... Cardiac sections were then washed in PBST and labeled with FITC conjugated Wheat-Germ Agglutinin (1:500; Vector Labs #FL-1021-5) and Goat anti-Mouse IgM Alexa Fluor 594 (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... tissue was rinsed again three times (5 minutes each) in 0.01M PBS before immersing in an avidin-biotin complex (ABC; Vector labs, PK-4000) in the same concentration PBS for 30 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... followed by washing in ultrapure water for 5 min and mounted with Vectashield Vibrance® Antifade Mounting Medium (# ZKO8O3, Vector Labs, US) with or without DAPI (0.001 mg/ml) ...
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bioRxiv - Cancer Biology 2024Quote: ... The incubation with the primary antibody is done for 5 hours followed by biotinylated mouse secondary (Vector Labs, MOM Kit BMK-2202) in 5.75μg/mL ...
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bioRxiv - Genetics 2021Quote: ... To reduce fluorescence fading most slides were mounted in Vectashield antifade mounting medium containing 4.6-diamidino-2-phenylindole (DAPI; Vector Laboratories). Slides stained with Alexa Fluor 660 were stained with DAPI and then mounted in the ProLongTM Gold Antifade Mountant (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... for 2 hrs at room temperature before 10 min staining with DAPI (1:10,000) and mounting in Vectashield (Vector Laboratories). Mean fluorescence was measured with Fiji (https://imagej.net/fiji ...
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bioRxiv - Microbiology 2020Quote: ... Coverslips were mounted using Vectashield mounting medium supplemented with 1 ug/ml 4,6-diamidino-2-phenylindole (DAPI; Vector Laboratories, Inc.). Slides were examined on a Zeiss Axiovert 200 microscope with an AxioCam camera and ZEN Pro software (Carl Zeiss ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were rinsed with TBS-T (0.1% Tween-20 in TBS) 2 times for 10 min and block with 1.5% horse serum (S-2000, Vector Laboratories) in TBS-T for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Floating sections were blocked for 1 hour at room temperature in 2% normal donkey serum (NDS, Vector Laboratories, Burlingame, CA) in 0.3% Triton X-100 PBS ...
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bioRxiv - Microbiology 2020Quote: ... sections were incubated for 2 hours with SNL-EBL conjugated to Cy5 (1:50 dilution, Vector Labs, cat # CL-1303) followed by 1 hour with Spn typing sera (1:2000 dilution ...
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bioRxiv - Neuroscience 2021Quote: ... They were then washed and incubated with biotinylated secondary antibody for 2 h at room temperature and developed with Elite ABC kit and 3,3-diaminobenzidine (Vector laboratories). Sections were mounted ...
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bioRxiv - Microbiology 2020Quote: ... 4,6-diamidino-2-phenylindole (DAPI) was added to the embedding medium (4:1 Citifluor AF1 (Citifluor) and Vectashield (Vector Laboratories)) ...
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bioRxiv - Neuroscience 2024Quote: ... washed and incubated in horse anti-mouse biotinylated secondary (1:200 in 2% BSA and 0.1% Triton {TBS]; Vector Laboratories) for 180 min prior to washing and development in Streptavidin-tertiary reagent (1:500 in 2% BSA and 0.1% Triton [TBS] ...
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bioRxiv - Neuroscience 2022Quote: ... and counterstained with 1 μg/mL DAPI (Thermo) for 2 minutes to label nuclei and mounted with VectaShield (Vector Laboratories). Images were taken using a confocal microscope (Zeiss LSM 800) ...
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bioRxiv - Molecular Biology 2023Quote: ... Double blocking was achieved with (1) H2O2 for 10 mins and (2) 2.5% normal horse serum for 1 hour (S-2012, Vector Labs). vWAT sections were incubated overnight at 4°C with primary antibody (CD68 ...
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bioRxiv - Neuroscience 2023Quote: ... for 2 h at RT followed by incubation in avidin-horseradish peroxidase complex (Vectastain ABC Elite, PK-6000; Vector Laboratories) for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue was then exposed to diaminobenzidine for 2 minutes using the DAB Peroxidase HRP Substrate Kit (Vector Laboratories, SK-4100) then washed with 1X PBS ...
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bioRxiv - Neuroscience 2024Quote: ... full-thickness colon segments of ∼1cm in length were blocked for 2 hrs in 1.5ml tubes containing 1 drop of Mouse-on-Mouse blocking reagent (MOM; Vector Labs) in 1ml of PBS with 0.5% Triton-X 100 ...
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bioRxiv - Neuroscience 2024Quote: ... Primary antibody was detected using a biotinylated secondary antibody (Table 2) by avidin-biotin peroxidase method (ABC Kit, Vector Laboratories) using diaminobenzidine (DAB ...
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bioRxiv - Biochemistry 2024Quote: ... the samples were washed four times with 1× PBS and mounted in Vectashield Vibrance antifade mounting media with 40,6-diamidino-2-phenylindole (DAPI; catalog no.: H-1800; Vector Laboratories). Images were acquired with a Zeiss LSM 880 confocal microscope with Airyscan ...
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bioRxiv - Cell Biology 2024Quote: ... Nuclei were stained using 49,6-diamidino-2-phenylindole (DAPI) and were subsequently overlaid with Vectashield (Vectashield; Vector Laboratories, Burlingame, CA) mounting medium for imaging with a Zeiss 900 confocal microscope with a 40X objective.
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bioRxiv - Systems Biology 2021Quote: ... #415190-9041-000) were treated with UV light for 1 h and coated with APES (3-aminopropyltriethoxysilane) using Vectabond reagent (Vector Laboratories, Burlingame, CA, USA, cat. #SP-1800-7) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... incubated in 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked in 5% normal goat serum (NGS) (Vector Labs, Burlingame, CA) with 0.1% Triton X-100 ...
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bioRxiv - Synthetic Biology 2021Quote: ... blocked in 5% bovine serum albumin for 30 min and probed for 1 h with fluorescein-labeled SBA (Vector Laboratories, catalog # FL-1011) and Alexa Fluor 647® (AF647)-conjugated anti-His antibody (R&D Systems ...
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bioRxiv - Cell Biology 2021Quote: ... the retinal sections were washed with PBS three times for 5 min each and then mounted with Vectashield Mounting Medium (Vector Laboratories, Burlingame, CA). The images were acquired with a BZ-X810 Keyence microscope (Keyence ...
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bioRxiv - Physiology 2021Quote: ... Sections were incubated at room temperature with 3% (v/v) H2O2 for 10 min to quench endogenous peroxidase activity and blocked by 5% (v/v) normal goat serum (Vector Laboratories, #S-1000) in PBS for 1 hour ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were rinsed again in PBS and then incubated for 1 hr in avidin-biotin complex (4.5 μL each of A and B reagents/ml PBS with 0.4% Triton-X 100, ABC Elite Kit, Vector Laboratories, Burlingame, CA, USA). After rinsing in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were rinsed again in PBS and then incubated for 1 hr in avidin-biotin complex (4.5 μL each of A and B reagents/ml PBS with 0.4% Triton-X 100, ABC Elite Kit, Vector Laboratories, Burlingame, CA, USA). After rinsing in PBS ...
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bioRxiv - Neuroscience 2020Quote: ... Another set of washes in PBS (3x–5 min each) were performed and then the slides were mounted using Vectashield with DAPI (Vector Labs, H-1200).
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bioRxiv - Immunology 2020Quote: ... 3 times with PBTD and counterstaining with DAPI in 5% NGS blocking solution took place after the first wash. Coverslips (No. 1) were mounted using Vectashield (Vector Laboratories, Burlingame, California) and sealed with nail polish ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then rinsed in PBS (5 x 5 min) followed by a 1 hour incubation in biotinylated goat anti-rabbit secondary (1:500 in PBS, Vector Laboratories, BA-1000). Sections were rinsed in PBS (5 x 5 min ...
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bioRxiv - Molecular Biology 2022Quote: ... ATAC-see samples were incubated for 1 hour at room temperature protected from light with blocking buffer (1X PBS, 0.1% TX-100 and 5% normal goat serum (Vector Laboratories, S-1000-20). ds/ssDNA mouse monoclonal IgM antibody clone AC-30-10 (PROGEN ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were again rinsed five times in PBS and then incubated for 1 hr in avidin-biotin complex (4.5 μL each of A and B reagents/ml PBS with 0.4% Triton-X 100, ABC Elite Kit, Vector Laboratories, Burlingame, CA, USA). After rinsing in PBS ...
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bioRxiv - Neuroscience 2024Quote: ... slides were rinsed three times for 5 minutes with 0.4% Triton-X-100 TBS and mounted using VectaShield® Hardset ™ Antifade Mounting Medium with DAPI (Vector Laboratories, California). Slides were then left alone to set for at least 48 hours at 4° C and imaged on a Leica DMi8 Confocal Microscope.
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bioRxiv - Cancer Biology 2023Quote: ... Cell nuclei were stained with spectral DAPI in PBS for 5 min and the slides were mounted with Hard-set Vectashield mounting medium (Vector Laboratories, Burlingame, CA). Mounted slides were allowed to harden prior to scanning.
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... followed by washing 3x in PBS for 5 minutes before 60-minute incubation with a Streptavidin-conjugated Dylight 488 secondary (1:200, Vector Laboratories, SA-5488). After secondary ...
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bioRxiv - Bioengineering 2023Quote: ... Slides were washed with 1X TBS twice again for 5 minutes each and Vectashield Mounting Media (H-1200; Vector Laboratories, Newark, CA, USA) was added before coverslipping ...
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bioRxiv - Pathology 2023Quote: ... Tissue sections (5 mm) from each block were cut in a cryostat at −21 C and mounted onto Vectabond-coated slides (Vector Laboratories, Peterborough, UK), fixed in cold acetone for 10 minutes and incubated with an anti PECAM-1 rat primary antibody (BD Biosciences ...
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bioRxiv - Neuroscience 2023Quote: ... The intracellular single unit recordings were performed on the L2/L3 DRGs using the quartz microelectrode containing 5% Neurobiotin (Vector Laboratories, Burlingame, CA) in 1M potassium acetate ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Haematoxylin and Eosin (H&E) staining was performed on 5-μm sections that were prepared and stained using kit (Vector Laboratories, CA, USA) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... We washed slides twice for 5 minutes in RNase-free 1X PBS and marked the sample perimeter of each slide with an ImmEdge pen (Vector Laboratories #H-4000) in between both washes ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were washed with PBS and either stained for 5 min with 1 µg/ml DAPI (for chamber slides) or mounted using DAPI-containing Vectashield (Vector Laboratories; for coverslips). An Olympus IX70 inverted microscope with a UPlanApo 100X/1.35 oil iris objective was used for image acquisition with Micro-Manager software (v2.0) ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated for two hours with a biotinylated horse anti-mouse antibody (1:500 in PBS containing 0.5% Triton X-100, Vector Laboratories, #BA-2001-.5), followed by three PBS washes ...