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Citations for Vector Labs :
851 - 900 of 965 citations for Recombinant Human IL6 Protein non tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Excess antibody buffer was removed with PBSTx and followed by incubation in biotinylated goat anti-mouse secondary antibody (1:1000 Cat# BP-9200-50: Vector Laboratories, California USA) overnight at 4°C in PBSTx and 1% NGS ...
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bioRxiv - Neuroscience 2022Quote: ... tissue sections incubated with biotinylated secondary antibody were washed and antigen/antibody complexes were visualized using Vectastain ABS peroxidase kit (Vector Laboratories, Burlingame, CA) followed by counterstaining with Harris hematoxylin ...
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bioRxiv - Neuroscience 2023Quote: ... slices were incubated overnight at 4°C with a solution containing biotinylated Wisteria floribunda Lectin (WFA, B-1355-2, Vector Laboratories, 1:200) and 3% BSA in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed 3 × 10 min with PBS and incubated in a tertiary of avidin and biotinylated horseradish peroxidase (1:1000; ABC kit, Vector Labs, PK-6100) and stained with a 3 ...
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bioRxiv - Cancer Biology 2022Quote: ... CDX tumors were grown to approximately 750 mm3 and mice received an i.v injection of Biotinylated Tomato Lectin (4mg/kg, Vector Laboratories, B-1175-1) one hour before they were sacrificed by Schedule 1 method ...
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bioRxiv - Neuroscience 2023Quote: ... or the corresponding secondary antibodies: biotinylated goat anti-guinea pig antibody (1:100 dilution for GFP detection; Vector Laboratories, Cat# PK-4007; RRID:AB_2336816) and secondary antibody goat anti-mouse IgG coupled to 1.4 nm gold (1:100 dilution for TH detection ...
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bioRxiv - Neuroscience 2023Quote: ... USA) followed by secondary biotinylated antibody incubation (1:200, goat anti-rabbit IgG, Cat. No. BA-1000; Vector Labs Inc., Burlingame, CA, USA). Controls for nonspecific binding consisted in omitting the primary antibody (S Fig 5) ...
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bioRxiv - Cancer Biology 2023Quote: ... sections were incubated with biotinylated secondary antibody for 30 minutes followed by ABC kit (VECTASTAIN Elite ABC-HRP kit, Vector Laboratories, PK-6100) for 30 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... Bound integrins were quantified by an enzyme-linked immunosorbent (ELISA)-like solid-phase assay using biotinylated rabbit anti-velcro (against ACIS/BASE coiled-coil) antibody and HRP-conjugated streptavidin (VECTOR Laboratories #SA-5004). After addition of ABTS ...
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bioRxiv - Neuroscience 2023Quote: ... 0.3% Triton X-100 (24 hours, 37°C) followed by peroxidase-conjugated secondary antibody (1:200 biotinylated anti-rabbit Vector Labs #BA-1000) and incubated in Elite ABC solution ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated for 2 h (at RT) with a secondary biotinylated antibody followed by an avidin-biotin-peroxidase complex (ABC; Vector Laboratories, Peterborough, UK) for 1 hour in PBS ...
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bioRxiv - Microbiology 2023Quote: ... The wells were then incubated in a primary antibody blocking solution containing rabbit anti-GAP45 or guinea pig anti-CDPK1 or biotinylated dolichos (Vector labs B-1035) for 60 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed three times in 0.1% Triton X-100 in TBS followed by 1 hr incubation with goat biotinylated anti-rabbit IgG secondary antibody (1:250, BA-1000, Vector Laboratories, RRID: AB_2313606) at room temperature and 30 min incubation with Vectastain Elite ABC reagent (PK-6100 ...
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bioRxiv - Cell Biology 2023Quote: ... tissue sections were incubated with the secondary biotinylated antibody corresponding to the animal species of the primary antibody (BA-1000; Vector Laboratories, Burlingame, CA) and exposed to 3,30-diaminobenzidine (SK-4105 ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed and incubated in the presence of biotinylated goat anti-rabbit IgG followed by Vectastain elite ABC reagent (1:600, #PK6101, Vector Laboratories, Burlingame, USA). Fos-like immunoreactive cells were visualized with the avidin-biotin peroxidase method using diaminobenzidine as a chromogen ...
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bioRxiv - Neuroscience 2021Quote: ... Non-specific binding sites were blocked for 1 hour with Mouse on Mouse (M.O.M.) Blocking reagent (Vector Laboratories; MKB-2213) followed by washing with TBS ...
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bioRxiv - Physiology 2022Quote: ... the sections were stained with DAPI and the slides were mounted using a non-fluorescing aqueous mounting medium (Vector Laboratories) and and images were captured using Nikon Eclipse Ti-2e microscope (Nikon ...
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bioRxiv - Neuroscience 2020Quote: ... The tissue was then washed in PBS and incubated for 1 hour in biotinylated anti-rabbit IgG (1:200; Vector Labs, Burlingame, CA, USA), rotating at RT ...
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bioRxiv - Neuroscience 2020Quote: ... retinas were washed in 0.5% TX-100 and incubated for 1.5 hours in anti-mouse biotinylated secondary antibody (1:400; Vector Laboratories; cat # BA-9200; RRID: AB_2336171). Washes were performed followed by treatment in VECTASTAIN Elite ABC HRP Kit (1:800 ...
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bioRxiv - Neuroscience 2020Quote: ... secondary antibodies (goat anti-rabbit, Vector Laboratories inc., PI-1000, 1:1,000 and biotinylated horse anti-mouse, Vector Laboratories inc., PK-4002, 1:200) were added to the sections for 150 min ...
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bioRxiv - Pathology 2021Quote: ... we performed immunohistochemical staining using the avidin-biotinylated enzyme complex method and the VECTASTAIN Elite ABC Rabbit IgG Kit (Vector Laboratories, Burlingame, CA, USA). The tissue sections were deparaffinized with xylene and ethanol ...
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bioRxiv - Neuroscience 2022Quote: ... This was followed by an incubation with biotinylated goat anti-rabbit secondary antibody (1:200 in 3% NGS + 0.1% Triton X-100; Vector Labs, Burlingame, CA; Lot# 140893). Next ...
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bioRxiv - Neuroscience 2022Quote: ... sections were washed 5x in 0.1M PBS for 10 min per wash and incubated overnight at 4°C in secondary antibody (biotinylated goat anti-rabbit IgG; 1:500; Vector Laboratories, Inc, Burlingame, CA). The last day ...
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bioRxiv - Bioengineering 2022Quote: ... primary monoclonal rat anti mouse Mac1 antibodies (AbD Serotec, Raleigh, NC, USA) were used along with secondary biotinylated goat anti-rat antibodies (Vector Laboratories, Burlingame, CA, USA). Each midbrain section was viewed at low power (10x objective) ...
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bioRxiv - Immunology 2022Quote: ... Detection of bound antibodies was performed with biotinylated secondary goat anti-rabbit antibody and ABC-Kit using the peroxidase substrate method DABImmPact (all from Vector Laboratories, Burlingame, CA, USA).
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bioRxiv - Neuroscience 2021Quote: ... followed by incubation overnight at 4 °C in secondary antibody diluted in block (1:500 goat anti-chicken Alexa 488, Life Technologies, A11039; 1:5000 biotinylated goat anti-rabbit, Vectastain Elite ABC HRP kit, PK-6101, Vector Laboratories, Burlingame, CA). Sections were washed several times in TBS with 0.3% Triton X-100 and 0.05% Tween 20 ...
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bioRxiv - Immunology 2022Quote: ... were incubated at 4°C overnight followed by washing and incubation with a secondary biotinylated goat anti-mouse IgG antibody (dilution: 1:200, Vector Laboratories, Burlingame, California, USA). For color development freshly prepared avidin-biotin-peroxidase complex (ABC ...
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bioRxiv - Immunology 2020Quote: ... and then washed three times in PBST before incubation with biotinylated-secondary antibodies dissolved in PBST (BA-1000 for anti-rabbit and BA-9200 for anti-mouse; Vector Laboratories, 1:1,000 dilution) at 25°C for 1 h ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were rinsed repeatedly with PBS and then incubated in 1% avidin-biotinylated horseradish peroxidase (Vector ABC staining kit, Vector Lab. Inc., Burlingame, USA) containing 0.1% Triton X-100 for 1 h at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Slices were rinsed repeatedly with PBS and then incubated in 1% avidin-biotinylated horseradish peroxidase (Vector ABC staining kit, Vector Lab. Inc., Burlingame, USA) containing 0.1% Triton X-100 for 1 h at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated at room temperature for 1.5 hours in secondary antibody solution containing either biotinylated rabbit anti-goat IgG (Vector Laboratories, Burlingame, CA; PK-6105) or biotinylated goat anti-rabbit IgG (Vector Laboratories ...
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bioRxiv - Cancer Biology 2022Quote: ... Cleaved caspase 8 immunohistochemistry staining was performed using cleaved caspase 8 primary antibody (1:100; Cell Signalling Technology, cat. #9496) and goat anti-rabbit biotinylated secondary (1:200; Vector Laboratories, cat. BA-1000). Briefly ...
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bioRxiv - Neuroscience 2022Quote: ... The tissue sections were incubated overnight at 4°C with primary rabbit anti-TH antibodies followed by staining with biotinylated anti-rabbit IgG and an avidin-biotin peroxidase complex (ABC) standard kit (Vector Laboratories, Burlingame, CA, USA). Signals were detected by incubating sections with 0.5 mg/ml 3,3′-diaminobenzidine (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... incubated in the appropriate biotinylated secondary antibody (goat anti-rabbit, #BA-100; goat anti-mouse, Vector Labs #BA-9200;Vector Laboratories, Burlington, ON Canada) and then incubated with avidin-biotin complex (Vectastain ABC kit ...
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bioRxiv - Microbiology 2022Quote: ... Removal of α(2,3)-linked and α(2,6)-linked sialic acids on the cell surface was verified by staining with biotinylated Maackia amurensis lectin (MAL) I (5 μg/ml; Vector Laboratories; B-1265-1) or fluorescein-labeled Sambucus nigra lectin (SNA ...
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bioRxiv - Neuroscience 2023Quote: ... incubated for 1 hour at room temperature in biotinylated anti-mouse secondary antibody at 1:500 dilution (Vector Laboratories; cat # BA-9200; RRID: AB_2336171), followed by incubation in ABC Media 1:800 (Vector Laboratories ...
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bioRxiv - Bioengineering 2023Quote: ... incubation with secondary antibodies (either anti-goat, anti-rabbit, or anti-mouse biotinylated secondary antibody, all from Vector Labs, California, USA, at 1:200), detection using a Vector Elite ABC kit (Vector) ...
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bioRxiv - Neuroscience 2023Quote: ... This was followed by incubation with biotinylated horse anti-mouse IgG secondary antibody (1:200, PK-6102, Vectastain Elite ABC HRP kit, Vector Laboratories, USA, RRID: AB_2336821) for 30 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... slices were rinsed several times using 100 mM PBS and subsequently incubated in 1% avidin-biotinylated horseradish peroxidase (Vector ABC staining kit, Vector Lab. Inc., Burlingame, USA) containing 0.1% Triton X-100 for 1 h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Incubation in primary antibody was followed by washes and a 2-hour incubation in anti- rabbit biotinylated secondary antibodies (1:200, Vector Laboratories, Burlingame, CA, RRID:AB_2313606). Sections were then washed and incubated for 1.5 hours in avidin- biotin complex solution (1:200 ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were then mounted on glass slides (VECTASHIELD antifade mounting medium, H-1000, or with DAPI for non-stained slices, H-1200, Vector Laboratories) and imaged using Zeiss Axio Scan Z1 slide scanner fluorescence microscope ...
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bioRxiv - Cell Biology 2020Quote: ... embryos were treated with AB solution for 30 min at R/T (Avidin-Biotinylated Horseradish Peroxidase H from Vectastain-ABC KIT of Vector Laboratories 1:200 in PBT).
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bioRxiv - Microbiology 2021Quote: ... For blotting experiments gels were transferred onto nitrocellulose and unspecific binding blocked using 5% BSA and 2% polyvinylpyrrolidone (PVP) for blot detection with α-DAF or biotinylated Sambucus nigra agglutinin (SNA) (Vector Laboratories B-1305-2), respectively ...
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bioRxiv - Neuroscience 2019Quote: ... sections were incubated in avidin-biotinylated horseradish peroxidase complex (6 mL/mL avidin and 6 mL/mL biotin; 2 hr at room temperature; Vector Laboratories, CA, USA, #PK-6100), washed in PB ...
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bioRxiv - Neuroscience 2019Quote: ... After overnight incubation in primary antibody,the sections were rinsed in washing buffer and then incubated for 1 h in an anti-rabbit biotinylated antibody (Vector Laboratories, #ba-9400; 1:200) in washing buffer containing 2% normal goat serum ...
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bioRxiv - Microbiology 2022Quote: ... the secondary biotinylated goat anti-mouse or anti-rabbit antibody was applied for 30 minutes at room temperature (Vector Laboratories, Burlingame, CA, USA, 1:200). Slides were scanned using Hamamatsu S60 scanner ...
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bioRxiv - Neuroscience 2020Quote: ... We then rinsed sections with PB and incubated for 1 h at room temperature in avidin-biotinylated horseradish peroxidase (Vector Laboratories; ABC kit; 1:100). Sections were rinsed ...
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bioRxiv - Microbiology 2022Quote: ... Slides were washed thrice with PBS to remove excess antibody and incubated with a rabbit anti-goat biotinylated IgG (Vector laboratories catalogue number BA-5000) for 10 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... Tissues were incubated with primary antibody overnight at room temperature and detected using the appropriate biotinylated secondary antibody (1:200, Vector Labs, BA-1100, BA-2000) and alkaline phosphatase-Vector Red according to manufacturer instructions (Vector Labs) ...
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Analysis of spike protein variants evolved in a novel mouse model of persistent SARS-CoV-2 infectionbioRxiv - Microbiology 2023Quote: ... The slides stained with rabbit anti-SARS-CoV-2 S polyclonal antibody or rabbit anti-ACE2 antibody were further incubated with biotinylated horse anti-rabbit antibody (Vector Laboratories, cat. no. BP 1100). All slides were then incubated with HRP-conjugated streptavidin (Vector Laboratories ...