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Citations for Vector Labs :
851 - 895 of 895 citations for 3' Chloro 3 3 chloro 5 fluorophenyl 4' fluoropropiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... Slides were washed with 1X TBS twice again for 5 minutes each and Vectashield Mounting Media (H-1200; Vector Laboratories, Newark, CA, USA) was added before coverslipping ...
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bioRxiv - Genetics 2023Quote: ... We washed slides twice for 5 minutes in RNase-free 1X PBS and marked the sample perimeter of each slide with an ImmEdge pen (Vector Laboratories #H-4000) in between both washes ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Haematoxylin and Eosin (H&E) staining was performed on 5-μm sections that were prepared and stained using kit (Vector Laboratories, CA, USA) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... slides were washed in TBS + 0.001 % Triton (2 x 5 min) and incubated with an ImmPress HRP secondary antibody (Vector Laboratories, MP-7401-15) for 1 h ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... followed by washing 3x in PBS for 5 minutes before 60-minute incubation with a Streptavidin-conjugated Dylight 488 secondary (1:200, Vector Laboratories, SA-5488). After secondary ...
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bioRxiv - Neuroscience 2024Quote: ... slides were rinsed three times for 5 minutes with 0.4% Triton-X-100 TBS and mounted using VectaShield® Hardset ™ Antifade Mounting Medium with DAPI (Vector Laboratories, California). Slides were then left alone to set for at least 48 hours at 4° C and imaged on a Leica DMi8 Confocal Microscope.
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bioRxiv - Cancer Biology 2023Quote: ... Cell nuclei were stained with spectral DAPI in PBS for 5 min and the slides were mounted with Hard-set Vectashield mounting medium (Vector Laboratories, Burlingame, CA). Mounted slides were allowed to harden prior to scanning.
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bioRxiv - Molecular Biology 2022Quote: ... ATAC-see samples were incubated for 1 hour at room temperature protected from light with blocking buffer (1X PBS, 0.1% TX-100 and 5% normal goat serum (Vector Laboratories, S-1000-20). ds/ssDNA mouse monoclonal IgM antibody clone AC-30-10 (PROGEN ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed with PBS-TX (0.5% Triton X-100 in PBS) 6 x 20 min then blocked in 5% normal goat serum (NGS; Vector Laboratories, Burlingame, CA, USA) for 1 h before being incubated on a shaker overnight with primary antibodies (Table 1 ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were again rinsed five times in PBS and then incubated for 1 hr in avidin-biotin complex (4.5 μL each of A and B reagents/ml PBS with 0.4% Triton-X 100, ABC Elite Kit, Vector Laboratories, Burlingame, CA, USA). After rinsing in PBS ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated for two hours with a biotinylated horse anti-mouse antibody (1:500 in PBS containing 0.5% Triton X-100, Vector Laboratories, #BA-2001-.5), followed by three PBS washes ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were washed with PBS and either stained for 5 min with 1 µg/ml DAPI (for chamber slides) or mounted using DAPI-containing Vectashield (Vector Laboratories; for coverslips). An Olympus IX70 inverted microscope with a UPlanApo 100X/1.35 oil iris objective was used for image acquisition with Micro-Manager software (v2.0) ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated with a rabbit polyclonal anti-syncollin antibody for 2 h at room temperature followed by an FITC-conjugated goat anti-rabbit secondary antibody and 4′,6-diamidino-2-phenylindole (DAPI, Vector Laboratories, Burlingame, CA; 1.5 μg.mL−1).
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bioRxiv - Microbiology 2022Quote: ... The treated samples were incubated with primary antibodies at 4°C overnight followed by universal HRP-conjugated secondary antibodies (Vectastain® Elite ABC-HRP kit, Vector Laboratories, Inc., Burlingame, CA, USA). Immunoreactivity was developed by incubating membrane filter with DAB (3,3-diaminobenzidine tetrahydrochloride ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated overnight at 4°C in slow agitation with primary antibody and/or biotinylated Wisteria Floribunda lectin (WFA, 1:1000, Vector Labs, USA, Ref. B-1355-2). The following primary antibodies were used ...
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bioRxiv - Molecular Biology 2021Quote: All slides were washed three times for 5 min at RT and dried before mounting with the DAPI-containing VectaMount Permanent Mounting Medium (Vector Laboratories, Burlingame, CA, USA), and sealed with nail varnish ...
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bioRxiv - Developmental Biology 2020Quote: ... Secondary antibody was decanted and slides washed three times with PBS for 5 minutes each followed by VECTASHIELD® Antifade Mounting Medium with DAPI (Vector Labs H-1200), sealing coverslip ...
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bioRxiv - Neuroscience 2021Quote: ... sections were washed two more times for 5 min in 1x PBS and mounted with Vectashield mounting media (Vector Laboratories, # H1000, Burlingame, CA, USA). The following primary antibodies and dilutions were used ...
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bioRxiv - Biochemistry 2020Quote: ... The membranes were blocked with 5% BSA and then incubated with biotin-labeled Maackia amurensis lectin II (MAL-II biotin conjugate, Vector Laboratories, Inc., Burlingame, CA) for 1 h at room temperature ...
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bioRxiv - Microbiology 2021Quote: Paraffin-embedded tissues were sectioned at 5 µm and subjected to IHC using Vectastain Elite ABC Peroxidase (HRP) Kit (Vector Laboratories Cat # PK-6102) as previously described [40] ...
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bioRxiv - Immunology 2022Quote: Clarified cell supernatant was adjusted to a final concentration of 100 mM NaCl and then incubated with Galanthus Nivalis Lectin Agarose (Vector Laboratories, AL-1243-5), washed with 10 column volumes of 20 mM Tris ...
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bioRxiv - Cell Biology 2022Quote: ... Endogenous peroxidase was blocked for 5 mins before incubation with 2.5% Normal Goat Serum (ImmPRESS Rat kit – Vector Laboratories, San Francisco Bay Area, USA) TRU for 20 mins ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were washed three times with 1X PBS for 5 minutes each wash and then mounted using a DAPI-staining mounting medium (Vector Laboratories; H-1200-10). Cells were imaged using Leica DMI4000B microscope fitted with Leica TCS SPE laser-scanning confocal system with Solid State lasers (405/488/561/635 ...
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bioRxiv - Microbiology 2021Quote: ... Worms were washed in PBS three times for 5 mins and transferred to new 12 well plates with 1ml of Fluorescein labeled Aleuria Aurantia Lectin (Vector labs cat# FL-1391) and Rhodamine labeled Griffonia Simplicifolia Lectin I (Vector labs cat# RL-1102 ...
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bioRxiv - Neuroscience 2023Quote: ... The blots were washed with 1X TBST thrice for 5 minutes and then incubated with HRP-conjugated secondary antibodies (1:4000, Vector Labs, Burlingame, CA, USA) for 1-2 hours at RT ...
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bioRxiv - Microbiology 2022Quote: ... Removal of α(2,3)-linked and α(2,6)-linked sialic acids on the cell surface was verified by staining with biotinylated Maackia amurensis lectin (MAL) I (5 μg/ml; Vector Laboratories; B-1265-1) or fluorescein-labeled Sambucus nigra lectin (SNA ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... the slides were washed three times with PBS (5 minutes each at RT) and mounted using Vectashield antifade mounting media with DAPI (Vector Laboratories, #H-1200-10). To secure the coverslips ...
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bioRxiv - Neuroscience 2022Quote: ... antigen retrieval was performed by using either formic acid for 5 minutes at room temperature or citric acid pH=6 (Vector Laboratories; Cat# H-3300) for 15 minutes at 95 °C ...
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bioRxiv - Neuroscience 2024Quote: ... sections were rinsed in PBS three times for 5 min each and mounted on slides with VECTASHIELD Antifade Mounting Medium with DAPI (Vector Laboratories, H-1200-10) and coverslipped ...
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bioRxiv - Pathology 2024Quote: ... sections were stained with Flash Phalloidin™ and autofluorescence quenched for 5 min with the Vector® TrueVIEW™ autofluorescence quenching kit (Vector Laboratories), then mounted with Prolong Diamond Antifade Mountant with DAPI ...
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bioRxiv - Cancer Biology 2024Quote: ... Slides were rinsed in distilled water and PBS for 5 minutes and then blocked in 10% normal goat serum (Vector Laboratories S-1000-20) in PBS ...
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bioRxiv - Developmental Biology 2024Quote: ... rehydrated and subjected to antigen retrieval using a pre-heated (5 minutes at 100% microwave power) 1/1000 dilution of citrate-based antigen unmasking solution (Vector Labs, H-3300-250) for 20 minutes in the microwave at 10% power ...
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bioRxiv - Microbiology 2021Quote: ... For blotting experiments gels were transferred onto nitrocellulose and unspecific binding blocked using 5% BSA and 2% polyvinylpyrrolidone (PVP) for blot detection with α-DAF or biotinylated Sambucus nigra agglutinin (SNA) (Vector Laboratories B-1305-2), respectively ...
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bioRxiv - Neuroscience 2021Quote: ... and then cleared in xylene for 5 min before mounting with antifade medium with DAPI (VectaShield® HardSet™ with DAPI, Vector Laboratories, Auckland, NZ) and a coverslip ...
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bioRxiv - Immunology 2020Quote: ... The SARS-CoV-2 RBD-His tag and S1 proteins were purified using Ni-NTA resin (Thermofischer, cat.no. 88221) and Agarose bound Galanthus Nivalis Lectin (GNL) (Vector Labs, cat. no. AL-1243-5) respectively ...
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bioRxiv - Immunology 2023Quote: Single chain HIV-1 gp140 SOSIP variant strain BG505 [52] was purified over agarose bound Galanthus nivalis lectin (Vector Laboratories cat no. AL-1243-5). Column was washed with PBS ...
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bioRxiv - Immunology 2021Quote: ... Supernatant was 0.45 µm filtered with PES membrane Nalgene Rapid Flow Disposable Filter Units and then run slowly over an affinity column of agarose bound Galanthus nivalis lectin (Vector Laboratories cat no. AL-1243-5) at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... three PBS washes were performed for 5 min each and the slides were incubated for 1 hour with the secondary antibody (Vector Lab, BA-1000, 1:200 dilution), followed by three PBS washes for 5 min each ...
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bioRxiv - Molecular Biology 2022Quote: ... The sections were then stained with 16μM Hoechst 33258 (ThermoFischer, H3569) for 5 minutes and washed with 1X PBS before mounting with VECTASHIELD® HardSet™ Antifade Mounting Medium (Vector Laboratories, H-1400-10). Stained slides were then visualized with a Leica SP8 confocal microscope ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were then washed thrice with DPBS before blocking and permeabilizing for one hour at room temperature with blocking buffer: DPBS supplemented with 5% Normal Goat Serum (Vector Laboratories; Cat. No. S-1000-20) with 0.01% Triton X-100 (TEKnova ...
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bioRxiv - Cell Biology 2024Quote: ... sections were washed in PBS-TT for 5 min twice then incubated with the secondary antibodies: biotinylated anti-rabbit IgG (Vector Laboratories, Burlingame, United States; 1:250) and Alexa Fluor 647 (Far red ...
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bioRxiv - Neuroscience 2020Quote: ... The next day the slides were rinsed 3 times for 5 minutes in 1X PBS and then incubated for 1 hour at RT with anti-rabbit biotinylated secondary antibody (Vector Laboratories, product no: BA-1000 (1:500)) diluted in blocking solution ...
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bioRxiv - Neuroscience 2024Quote: ... to quench endogenous peroxidases, washed and blocked in 5% normal goat serum (Linaris, 69221; Dossenheim Germany, S-1000) and bovine serum albumin (Vector Laboratories, Newark, CA 94560, USA; SP-5050) in PBST ...
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bioRxiv - Developmental Biology 2024Quote: ... washed again 3 times for 5 minutes at RT with PBS and mounted with Vectashield (with or without DAPI, depending on experiment – Vector Laboratories H-1000-10 & H-1200-10).
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Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: Fluorescein-conjugated Sambucus nigra lectin (SNA) that binds sialic acid and Erythrina cristagalli lectin (ECL) that binds galactose were acquired from Vector Laboratories (SA-5001-1 and FL-1141-5, respectively).