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Citations for Vector Labs :
801 - 842 of 842 citations for Recombinant Rabbit IL5 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... followed by incubation with biotin-conjugated anti-rabbit antibody or biotin-conjugated anti-mouse antibody (Vectastain Elite ABC Kit, Vector Laboratories, Burlingame, CA, USA). After triple washing steps ...
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bioRxiv - Neuroscience 2021Quote: ... followed by incubation overnight at 4 °C in secondary antibody diluted in block (1:500 goat anti-chicken Alexa 488, Life Technologies, A11039; 1:5000 biotinylated goat anti-rabbit, Vectastain Elite ABC HRP kit, PK-6101, Vector Laboratories, Burlingame, CA). Sections were washed several times in TBS with 0.3% Triton X-100 and 0.05% Tween 20 ...
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bioRxiv - Immunology 2020Quote: ... and then washed three times in PBST before incubation with biotinylated-secondary antibodies dissolved in PBST (BA-1000 for anti-rabbit and BA-9200 for anti-mouse; Vector Laboratories, 1:1,000 dilution) at 25°C for 1 h ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were then rinsed and incubated in secondary antibody solution for 24h at 4°C: 1:1000 rabbit anti-goat (Vector Laboratories, Burlington, ON, Canada) in 0.1M PBS ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated at room temperature for 1.5 hours in secondary antibody solution containing either biotinylated rabbit anti-goat IgG (Vector Laboratories, Burlingame, CA; PK-6105) or biotinylated goat anti-rabbit IgG (Vector Laboratories ...
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bioRxiv - Developmental Biology 2022Quote: ... immunofluorescence was performed on paraffin sections using antibodies in Supplemental Table 1b and the following reagents: ImmPRESS(r) HRP Horse Anti-Rabbit IgG Polymer Detection Kit (Vector Labs, MP-7401-50), ImmPRESS(r ...
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bioRxiv - Cancer Biology 2022Quote: ... Cleaved caspase 8 immunohistochemistry staining was performed using cleaved caspase 8 primary antibody (1:100; Cell Signalling Technology, cat. #9496) and goat anti-rabbit biotinylated secondary (1:200; Vector Laboratories, cat. BA-1000). Briefly ...
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bioRxiv - Neuroscience 2022Quote: ... The tissue sections were incubated overnight at 4°C with primary rabbit anti-TH antibodies followed by staining with biotinylated anti-rabbit IgG and an avidin-biotin peroxidase complex (ABC) standard kit (Vector Laboratories, Burlingame, CA, USA). Signals were detected by incubating sections with 0.5 mg/ml 3,3′-diaminobenzidine (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... Incubation in primary antibody was followed by washes and a 2-hour incubation in anti- rabbit biotinylated secondary antibodies (1:200, Vector Laboratories, Burlingame, CA, RRID:AB_2313606). Sections were then washed and incubated for 1.5 hours in avidin- biotin complex solution (1:200 ...
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bioRxiv - Neuroscience 2023Quote: ... incubated in the appropriate biotinylated secondary antibody (goat anti-rabbit, #BA-100; goat anti-mouse, Vector Labs #BA-9200;Vector Laboratories, Burlington, ON Canada) and then incubated with avidin-biotin complex (Vectastain ABC kit ...
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bioRxiv - Neuroscience 2023Quote: For light and electron microscopic analysis when we visualized the L5 fibers in the brainstem after the anti-mCherry primary antibody we used anti-rabbit ImmPRESS (1:2, Vector Laboratories Burlingame, Ca 94010) and DAB-Ni/DAB (DAB-Ni ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissues were incubated with primary antibody overnight at 4°C then exposed to biotinylated anti-rabbit secondary antibody (Vector Labs, BA-1000, 1:400 dilution, 30Dmin at room temperature followed by avidin-biotin complex formation (Vector Laboratories, # PK-6100), staining with DAB substrate (Vector Laboratories ...
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bioRxiv - Pathology 2023Quote: ... the sections were incubated for 30 min with a secondary antibody (peroxidase-labeled anti-mouse/anti-rabbit IgG (ImmPRESS kit, Vector Labs, Burlingame, CA, USA)) ...
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bioRxiv - Bioengineering 2023Quote: ... incubation with secondary antibodies (either anti-goat, anti-rabbit, or anti-mouse biotinylated secondary antibody, all from Vector Labs, California, USA, at 1:200), detection using a Vector Elite ABC kit (Vector) ...
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bioRxiv - Developmental Biology 2023Quote: ... sections were incubated in Tris-buffered saline/0.1% Tween-20 (TBS-T) containing 2.5% goat serum (included in ImmPRESS HRP Goat Anti-Rabbit IgG Polymer Kit, Vector Laboratories, MP-MP-7451-15) to block non-specific binding ...
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bioRxiv - Cancer Biology 2024Quote: ... and then stained with secondary anti-mouse IgG and anti-rabbit IgG HRP-conjugated antibodies for 30 min at RT (Vector Laboratories, MP-7500-50). After washing twice in washing buffer for 5 min each ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and incubated for 2-3 hours with biotinylated anti-rabbit secondary antibody (1:450 for DeltaFosB and 1:600 for c-Fos, Vector Laboratories, Burlingame, CA, USA). Finally ...
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bioRxiv - Neuroscience 2024Quote: ... for 30 min, a first antibody solution (PB 0.1M, Triton 0.3%, NGS 2%, Rabbit α-Phal-L 1:8000 (Vector Laboratories, AS-2300, RRID: AB_2313686), Mouse α-PV 1:4000 (Swant ...
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bioRxiv - Cancer Biology 2024Quote: ... Slides were washed with PBS and incubated for 1 h at room temperature with a protein-blocking solution (VECTASTAIN ABC kit, Vector Laboratories, Burlingame, CA, USA). Excess blocking solution was drained ...
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bioRxiv - Neuroscience 2019Quote: ... After overnight incubation in primary antibody,the sections were rinsed in washing buffer and then incubated for 1 h in an anti-rabbit biotinylated antibody (Vector Laboratories, #ba-9400; 1:200) in washing buffer containing 2% normal goat serum ...
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bioRxiv - Microbiology 2020Quote: ... The detection of antibody binding was performed with anti-rabbit IgG HRP-conjugated polymer (Zytomed GmbH, Berlin, Germany) or anti-rat IgG HRP-conjugated polymer ImmPRESS™ (Vector Laboratories, Burlingame, CA, USA) and 3-amino-9-ethylcarbazole (AEC ...
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bioRxiv - Cancer Biology 2020Quote: ... Primary antibodies were incubated with sections overnight at 4°C and detected using Vectastain Elite ABC HRP Kits (Vector Laboratories: Peroxidase Rabbit IgG PK-6101) and DAB substrate (Vector Laboratories ...
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bioRxiv - Pathology 2022Quote: ... overnight at 4°C then rinsed with TBST prior to detection using an anti-rabbit avidin-biotin amplification kit (Vector Laboratories Cat# PK-6101, RRID:AB_2336820). Vector NovaRed ...
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bioRxiv - Microbiology 2022Quote: ... the secondary biotinylated goat anti-mouse or anti-rabbit antibody was applied for 30 minutes at room temperature (Vector Laboratories, Burlingame, CA, USA, 1:200). Slides were scanned using Hamamatsu S60 scanner ...
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bioRxiv - Neuroscience 2019Quote: ... sections were incubated for 1 hr at room temperature in a biotinylated solution (biotinylated goat anti-rabbit IgG; 1:200 dilution; Vector Laboratories; catalog no. BA-1000) before incubating in an avidin-biotin horseradish peroxidase solution for 2 hrs at room temperature (Vector Novoxastra Laboratories) ...
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bioRxiv - Microbiology 2022Quote: ... Slides were washed thrice with PBS to remove excess antibody and incubated with a rabbit anti-goat biotinylated IgG (Vector laboratories catalogue number BA-5000) for 10 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... The second primary was added to the sections overnight at 4°C then detected with a goat α-rabbit DyLight® 488 (DI1488, Vector Laboratories, 1:200) and mounted using VECTASHIELD® Antifade Mounting Medium with DAPI (H-1200-10 ...
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bioRxiv - Immunology 2023Quote: ... primary antibody in Leica Primary Antibody Diluent for 30 min was followed by application of a rabbit anti-rat secondary (Vector Laboratories, Catalog Number AI-4001) for 8 min ...
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bioRxiv - Developmental Biology 2024Quote: ... immunofluorescence was performed on paraffin sections using antibodies in Supplementary Table 2 and the following reagents: ImmPRESS® HRP Horse Anti-Rabbit IgG Polymer Detection Kit (Vector Labs, MP-7401-50). Following the application of TSA fluorophores (listed in Supplementary Table 2 ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were washed with PBS (3 x 10 min) and incubated with biotinylated goat anti-rabbit secondary antibody (1:600, Vector Laboratories, Cat# BA-1000, RRID:AB_2313606), diluted in 1% NGS in PBS with Triton X-100 ...
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bioRxiv - Cell Biology 2024Quote: Secondary antibodies were Horse Radish Peroxidase conjugated goat anti-rabbit or anti-mouse (ImmPRESS Polymer Detection Kits, Peroxidase, Vector Laboratories, MP-7451 and MP-7452) or Dako REAL EnVision detection system (Dako ...
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bioRxiv - Pathology 2024Quote: ... the primary antibody was detected using the ImmPRESS® HRP Horse Anti-Rabbit IgG PLUS Polymer Kit (Vector Laboratories, MP-7801-15, Newark, California, USA) following manufacturer instructions.
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bioRxiv - Biochemistry 2022Quote: ... high-mannose N-glycosylated proteins were detected employing ConA-biotin (Galab, Hamburg, Germany) and the VECTASTAIN ABC-HRP Kit (Vector Laboratories, Burlingame, CA, United States).
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bioRxiv - Immunology 2020Quote: ... The SARS-CoV-2 RBD-His tag and S1 proteins were purified using Ni-NTA resin (Thermofischer, cat.no. 88221) and Agarose bound Galanthus Nivalis Lectin (GNL) (Vector Labs, cat. no. AL-1243-5) respectively ...
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Targeting MOG to skin macrophages prevents EAE in macaques through TGFβ-induced peripheral tolerancebioRxiv - Immunology 2019Quote: ... Incubation with primary antibodies was followed by a biotinylated goat-anti-rabbit antibody for 30 min followed by the avidin–biotin–peroxidase complex (Vectastain Elite ABC Kit, Vector Laboratories, PK 6100; Burlingame, CA, USA) for 30 min at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated with a rabbit polyclonal anti-syncollin antibody for 2 h at room temperature followed by an FITC-conjugated goat anti-rabbit secondary antibody and 4′,6-diamidino-2-phenylindole (DAPI, Vector Laboratories, Burlingame, CA; 1.5 μg.mL−1).
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bioRxiv - Neuroscience 2021Quote: ... sections were washed in PBST and incubated for 60 minutes at room temperature with a secondary biotinylated goat anti-rabbit antibody (Vector laboratories, Cat# BA-1000, lot# 2F0430) diluted 1:1000 ...
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bioRxiv - Molecular Biology 2023Quote: ... slides were incubated for 1 hour with a secondary antibody cocktail: 1:250 anti-rabbit IgG Texas Red 594 (Vector Labs, Newark, CA, USA; TI-1000) + anti-mouse IgG2b AF488 (Thermo Fisher Scientific ...
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bioRxiv - Pathology 2023Quote: ... sections were incubated with biotinylated anti-mouse or anti-rabbit secondary antibody and then with avidin-biotin complex using the Elite Vectastain kit (Vector Laboratories, PK-6101 and PK-6102). Chromogen reactions were performed using diaminobenzidine (SIGMAFAST DAB ...
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bioRxiv - Cancer Biology 2022Quote: ... sections were incubated for 1 hr with species-specific Horseradish Peroxidase (HRP)-conjugated secondary antibodies (ImmPRESS HRP Goat Anti-Mouse or Rabbit IgG Polymer Detection Kit, Peroxidase, Vector Laboratories, Cat. #: MP-7452 and MP-7451) before developing positive staining with ImmPACT DAB Substrate Peroxidase (HRP ...
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bioRxiv - Neuroscience 2020Quote: ... The next day the slides were rinsed 3 times for 5 minutes in 1X PBS and then incubated for 1 hour at RT with anti-rabbit biotinylated secondary antibody (Vector Laboratories, product no: BA-1000 (1:500)) diluted in blocking solution ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... followed by treatment with secondary biotinylated rabbit anti-goat IgG or rabbit anti-mouse IgG (30 min, room temperature) and peroxidase-conjugated avidin (VECTASTAIN® Elite ABC-Kit; Vector Laboratories, Burlingame, CA; another 30 min). The chromogen 3-amino-9-ethylcarbazole (AEC Substrate Pack ...