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Citations for Vector Labs :
801 - 848 of 848 citations for 3RS 4 Dimethylamino 3 methyl 2 2 diphenylbutanenitrile Isodidiavalo since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Sections were rinsed 3 × 5 min in TBS buffer and then incubated for 100 min in a solution of streptavidin Dylight 649 (Vector laboratories, SA5649, 1:100) in TBS buffer ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated for 1h at room temperature in a solution of 3% normal horse serum (for monoclonal antibodies; Vector Laboratories Inc., Burlingame, CA) and 0.25% Triton-X (Merck ...
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bioRxiv - Neuroscience 2022Quote: ... This was followed by an incubation with biotinylated goat anti-rabbit secondary antibody (1:200 in 3% NGS + 0.1% Triton X-100; Vector Labs, Burlingame, CA; Lot# 140893). Next ...
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bioRxiv - Neuroscience 2020Quote: ... and then incubated for 1h at room temperature in a solution of 3% normal horse serum (for polyclonal antisera and monoclonal antibodies, respectively; Vector Laboratories Inc., Burlingame, CA) and 0.25% Triton-X (Merck ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were then washed 3 times and treated with avidin-biotin complex solution (VECTASTAIN® Elite ABC-HRP Kit, Vector Lab, #PK-6100) for 90 min at RT ...
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bioRxiv - Neuroscience 2021Quote: ... Following one (cell lines) or 3 days expression (primary hippocampal cultures) cells were fixed using paraformaldehyde and coverslips mounted using Vectashield (Vector Laboratories, Burlingame, CA, USA) with or without DAPI (cell lines) ...
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bioRxiv - Neuroscience 2023Quote: ... then reacted for visualization of peroxidase with nickel-cobalt intensified diaminobenzidine for 3 - 8 minutes (DAB Peroxidase substrate kit SK-4100, Vector Laboratories, Burlingame, CA, USA). Brain slices were washed between each incubation step using TBS (3 × 10 minutes ...
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bioRxiv - Physiology 2022Quote: ... cells were washed 3 times with PBS on ice and bound biotinyl tyramide was visualized with fluorescent streptavidin (Vector Laboratories, Inc., Burlingame, CA, USA) at 1:1000 in PBS for 30 minutes at 4 °C ...
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bioRxiv - Developmental Biology 2024Quote: ... Tissues were then washed 3 times for 5 minutes each in PT and mounted in Vectashield with DAPI (Vector Laboratories; H-1200-314 10).
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bioRxiv - Bioengineering 2020Quote: ... beads were stored at 4°C in PBS to reduce background before mounting in VECTASHIELD antifade mounting media (Vector Laboratories H-1000). Images were acquired on a confocal laser scanning microscope (Leica SP5) ...
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bioRxiv - Neuroscience 2020Quote: ... was incubated overnight at 4 °C in blocking solution and biotinylated anti-rabbit IgG secondary antibody (1:3000, Vector Laboratories BA-1000) at RT for 1h ...
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bioRxiv - Biophysics 2021Quote: ... The cells were subsequently fixed in 4% paraformaldehyde for 15 minutes and the coverslips were mounted with Vectashield + DAPI (Vector Laboratories, USA) on a glass slide ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were rinsed in 0.01 PBS and incubated overnight at 4 °C with Alexa biotinylated goat anti-rabbit 488 (1:2000, Vector Laboratories, Burlingame, CA). After rinses ...
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bioRxiv - Neuroscience 2022Quote: ... Delipidated sections in PBS were blocked with BSA 4 % and incubated with a 50 ug/ml solution of lectin conjugated with fluorescein (Vector Laboratories, FL1171), 24 h at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... samples were washed 4×15 min with 0.2 % Tween-20 in PHEM buffer and mounted using Vectashield mounting media (Vector Laboratories, H-1000). Imaging was performed using a SP8 Leica confocal microscope with a 40 x objective with 1x digital zoom ...
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bioRxiv - Neuroscience 2024Quote: ... The sections were washed 4 times with PBST and mounted using VectaShield Hardset Antifade mounting medium (Vector Laboratories, Cat#H-1700-10). Images were taken using a Keyence BZ-810 all-in-one fluorescence microscope or a Zeiss LSM 700 inverted confocal microscope.
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bioRxiv - Neuroscience 2024Quote: ... After another washing session (6 × 20 minutes at 4°C) the retinae were embedded in mounting media (Vectashield, Vector Laboratories Inc., USA) on a glass slide ...
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bioRxiv - Cancer Biology 2024Quote: ... They were then blocked with 10% goat serum in PBS-Triton 0.1% (T-PBS) containing 4 drops/ml of Avidin solution (of the Avidin/Biotin Blocking Kit, Vector Labs SP-2001) for 1 hour and then incubated overnight with the primary antibody (Table 1 ...
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bioRxiv - Developmental Biology 2024Quote: ... De-waxed and rehydrated sections were heated up and then pressure cooked for 4 minutes in antigen unmasking solution (H-3300, Vector Laboratories Inc). Once cooled ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were washed with PBS (3 x 10 min) and incubated with biotinylated goat anti-rabbit secondary antibody (1:600, Vector Laboratories, Cat# BA-1000, RRID:AB_2313606), diluted in 1% NGS in PBS with Triton X-100 ...
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bioRxiv - Neuroscience 2024Quote: ... 3 x 10min PBS washes were followed by mounting in VECTASHIELD® HardSet™ mounting medium with DAPI (Vector Labs, UK, Cat# H-1500-10).
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bioRxiv - Neuroscience 2024Quote: ... sections were washed in PBS 3 more times (10 min, room temperature) and mounted to microscope slides using VECTASHIELD Antifade Mounting Medium with DAPI (Vector Laboratories, cat. # H-1200-10). Sections were imaged on a Nikon A1R confocal microscope at the Tufts University Center for Neuroscience Research Microscopy Core ...
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bioRxiv - Immunology 2022Quote: ... The second primary antibody was added overnight at 4°C and visualised the following day using Vector Red Substrate Kit (Vector Labs, SK-5100). Slides were counterstained with haematoxylin ...
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bioRxiv - Neuroscience 2020Quote: ... tissue sections were rinsed and incubated at room temperature in a secondary biotinylated anti-mouse antibody for 4 hours (Vector Laboratories, Burlingame, CA). This series was then incubated with ABC solution and reacted with Vector SG (Vector Laboratories ...
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bioRxiv - Bioengineering 2020Quote: ... gels were fixed with 4% paraformaldehyde and stained with 10 mg/mL rhodamine-labeled Griffonia simplicifolia lectin I (GS-1lectin; Vector Laboratories; Burlingame, CA). Five randomly selected fields were imaged per gel to an area of 3×3 mm and a depth of ∼500 µm using a Zeiss LSM 700 confocal microscope ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated overnight at 4 °C with STL-FITC (dilution 1:750, solanum tuberosum lectin-fluorescein isothiocyanate, endothelial marker, Vector Laboratories FL-1161) and goat anti-IBA1 (dilution 1:1500 ...
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bioRxiv - Physiology 2023Quote: ... the cells were fixed with 4% PFA at 4 ℃ for 20 minutes in the dark and mounted using VECTASHIELD® HardSet™ Antifade Mounting Medium (#H-1400-10, Vector Laboratories). Cell nuclei were stained with DAPI ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were incubated with primary antibodies overnight at 4°C and with secondary antibodies for 1 hr at room temperature (RT) before ABC-HRP (Vector Laboratories #PK-7100) treatment and DAB (Vector Laboratories #sk-4105 ...
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bioRxiv - Cell Biology 2023Quote: ... melanogaster larval imaginal discs were dissected in PBS and fixed in 4% paraformaldehyde and either mounted directly in VectaShield Mounting Medium (Vector Laboratories, H-1000) or first permeabilised with PBST (PBS with 0.3% Triton X-100 ...
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bioRxiv - Developmental Biology 2023Quote: ... in 1% NDS in PBS/MT at 4°C for 24 hr and subjected to peroxidase-conjugated streptavidin treatment in blocking solution (1:500; PK6200, Vector Laboratories, Burlingame, CA) at 4°C for 24 hr after rinsing in PBS/MT on ice (1 hr x 5) ...
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bioRxiv - Cell Biology 2024Quote: ... Cultures were subsequently fixed in 4% PFA and stained for ALP using the Vector Blue Alkaline Phosphatase Substrate Kit (Vector Laboratories SK-5300) and Von Kossa using a 4% silver nitrate solution and exposing cultures to 2400 kJ of ultraviolet light using a UV Stratalinker.
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bioRxiv - Physiology 2024Quote: ... mouse pancreatic sections were incubated overnight at 4°C in guinea pig anti-insulin antibody (1:800) and visualized using the diaminobenzidine peroxidase substrate (Vector laboratories, Burlingame, CA) and hematoxylin staining ...
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bioRxiv - Neuroscience 2021Quote: ... The membranes were then washed in PBST (3×5 minutes) before incubation with a relevant HRP-conjugated secondary antibody as appropriate (Vector Labs, UK; 1:1000, 1 h, RT). The signal was developed ...
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bioRxiv - Bioengineering 2021Quote: ... Mounting was conducted by incubating for 16 h at 4 °C with VECTASHIELD® PLUS Antifade Mounting Medium (H-1900; Vector Laboratories, Burlingame, CA). Extended information detailing primary antibody ...
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bioRxiv - Neuroscience 2022Quote: ... sections were washed 5x in 0.1M PBS for 10 min per wash and incubated overnight at 4°C in secondary antibody (biotinylated goat anti-rabbit IgG; 1:500; Vector Laboratories, Inc, Burlingame, CA). The last day ...
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bioRxiv - Neuroscience 2022Quote: ... slices were rinsed again 4 times for 5 minutes in PBS and revealed using a DAB substrate kit (Vector Laboratories Cat# SK-4100, RRID:AB_2336382). Stained slices (at least 5 per region ...
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bioRxiv - Microbiology 2022Quote: ... The cells were washed twice with DPBS before the coverslips were mounted on to a glass slide with 4 μl Vectashield with DAPI (Vector Laboratories, cat# H-1200) and sealed with clear nail polish ...
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bioRxiv - Cancer Biology 2023Quote: ... Autofluorescence in tissue sections due to aldehyde fixation was removed by treatment with the TrueVIEW Autofluorescence Quenching Kit for 4 min (Vector Laboratories; SP-8400-15). Sections were counterstained with DAPI (ThermoFisher 62247 ...
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bioRxiv - Microbiology 2024Quote: ... and mounted on a glass microscope slide using a 4:1 mixture of Citiflour™ AFI mountant solution to Vectashield® vibrance antifade mounting medium (Vector Laboratories, USA). The fluorescence images were taken using an Echo Revolve R4K (Upright & Inverted Capability ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Slides were incubated with the primary antibody overnight at 4 °C and processed using the VECTASTAIN® Elite ABC-HRP Rabbit IgG Kit (Vector Laboratories, #PK-6101) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Cryosections were blocked in PBS containing 10% donkey serum and 0.1% Triton X-100 for 2 h and incubated overnight at 4°C with rabbit anti-Iba-1 and goat anti-Arg1 antibodies or FITC-conjugated Lycopersicon esculentum (Tomato) Lectin (Vector Labs, FL-1171-1). Next ...
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bioRxiv - Systems Biology 2021Quote: ... #415190-9041-000) were treated with UV light for 1 h and coated with APES (3-aminopropyltriethoxysilane) using Vectabond reagent (Vector Laboratories, Burlingame, CA, USA, cat. #SP-1800-7) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... Primary antibodies were incubated with sections overnight at 4°C and detected using Vectastain Elite ABC HRP Kits (Vector Laboratories: Peroxidase Rabbit IgG PK-6101) and DAB substrate (Vector Laboratories ...
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bioRxiv - Pathology 2022Quote: ... overnight at 4°C then rinsed with TBST prior to detection using an anti-rabbit avidin-biotin amplification kit (Vector Laboratories Cat# PK-6101, RRID:AB_2336820). Vector NovaRed ...
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bioRxiv - Microbiology 2021Quote: ... the filter pieces were embedded in a 4:1 mix of Citifluor (Citifluor Ltd, London, United Kingdom) and Vectashield (Vector Laboratories, Inc., Burlingame, United States), and stored overnight at −20°C for later microscopy evaluation.
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bioRxiv - Developmental Biology 2022Quote: ... The second primary was added to the sections overnight at 4°C then detected with a goat α-rabbit DyLight® 488 (DI1488, Vector Laboratories, 1:200) and mounted using VECTASHIELD® Antifade Mounting Medium with DAPI (H-1200-10 ...
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bioRxiv - Microbiology 2022Quote: ... The treated samples were incubated with primary antibodies at 4°C overnight followed by universal HRP-conjugated secondary antibodies (Vectastain® Elite ABC-HRP kit, Vector Laboratories, Inc., Burlingame, CA, USA). Immunoreactivity was developed by incubating membrane filter with DAB (3,3-diaminobenzidine tetrahydrochloride ...
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bioRxiv - Neuroscience 2021Quote: ... Then the sections were rinsed with 1× PBS (3 × 10 min) and incubated with avidin–biotin–peroxidase complex (ABC Elite, Vector Laboratories, Germany; 0.2% A and 0.2% B in 1× PBS) at RT (1.5 h) ...