Labshake search
Citations for Vector Labs :
751 - 800 of 2053 citations for 5 MORPHOLIN 4 YLPENT 3 YN 1 OL since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2022Quote: ... staining was 2% goat serumPrimary antibodies were left overnight in 4 degrees and followed by anti-rabbit secondary (Vector Laboratories Cat# BA-1000). Next ...
-
bioRxiv - Neuroscience 2023Quote: ... for 40 seconds and sections were finally coverslipped with Vectashield mounting medium containing 4′,6-diamidino-2-phenylindole (DAPI) to label the nucleus (Vector Laboratories, H-1800). Slides were kept at 4 °C until imaging ...
-
bioRxiv - Physiology 2023Quote: ... the cells were fixed with 4% PFA at 4 ℃ for 20 minutes in the dark and mounted using VECTASHIELD® HardSet™ Antifade Mounting Medium (#H-1400-10, Vector Laboratories). Cell nuclei were stained with DAPI ...
-
bioRxiv - Physiology 2023Quote: ... the sections were mounted with hard-set DAPI (4′, 6-diamidino-2-phenylindole –hard set; Cat# H-1500, Vector Laboratories Ltd., Peterborough, UK). The slides were left overnight in the dark at 4°C ...
-
bioRxiv - Cancer Biology 2022Quote: ... The sections were washed with PBS and mounted using VECTASHIELD® Vibrance™ mounting medium with 4′,6-diamidino-2-phenylindole (DAPI, Vector Laboratories). Table S6 lists the primary and secondary antibody dilutions for the organ tissues ...
-
bioRxiv - Physiology 2023Quote: ... The coverslips were washed in PBS before being mounted onto a microscope slide with 4’,6-diamidino-2-phenylindole (DAPI) (Vectashield, Vector Laboratories, Burlingame, CA) mounting medium.
-
bioRxiv - Cell Biology 2023Quote: ... melanogaster larval imaginal discs were dissected in PBS and fixed in 4% paraformaldehyde and either mounted directly in VectaShield Mounting Medium (Vector Laboratories, H-1000) or first permeabilised with PBST (PBS with 0.3% Triton X-100 ...
-
bioRxiv - Cell Biology 2020Quote: ... 0.5% TritonX-100 in PBS) for 30 min at RT followed by another blocking using M.O.M kit (Vector Laboratories BMK-2202). Primary antibodies (mouse anti-MYH3 ...
-
bioRxiv - Cell Biology 2020Quote: ... The organoids were centrifuged at 500 x g for 5 min and resuspended in 30 μL Vectashield (Vector Labs; H-1000) using a cut pipet tip and pipetted onto a coverslide ...
-
bioRxiv - Genetics 2021Quote: ... slides were washed 3 times in PBS-T for 5 min each and incubated with the biotinylated horse anti-mouse IgG antibody (AK-5002, Vector laboratories) for 30 min ...
-
bioRxiv - Cell Biology 2019Quote: ... Cells were then blocked in a mix of 3% v/v BSA in PBS with 5% v/v donkey serum and Mouse on Mouse (M.O.M) blocking agent (Vector Laboratories MKB-2213), for 2 hours ...
-
bioRxiv - Biochemistry 2021Quote: ... and treated with or without PNGase F was assayed by lectin blotting using Concanavalin A (Con A) (Cat. B-1005-5, Vector Laboratories). A total of 10 μg protein each were electrophoresed in a 12% SDS-PAGE gel and transferred onto PVDF membranes at a constant of 300 mA for 2 h ...
-
bioRxiv - Genetics 2021Quote: ... then blocked with 5% (v/v) horse serum (ImmPRESS HRP Anti-Rabbit IgG [Peroxidase] Polymer Detection Kit; Vector labs; MP-7401). Sections were incubated with rabbit anti-TCFL5 (Sigma ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... DNMT3A and DNMT3B was visualized in cross-section (5 µm) of paraffin-embedded tissues using specific primary antibodies and a Vectastain Elite ABC Kit (Vector Laboratories) or AlexaFluor 488-conjugated secondary antibodies (Invitrogen) ...
-
bioRxiv - Neuroscience 2022Quote: Sections were briefly dried then washed 3x in 0.1% Triton X-100 in phosphate buffered saline (PBST) before they were blocked with 5% neutral goat serum (Abcam or Vector Labs). Sections were then incubated for double-labeling experiments with primary antibody (AB ...
-
bioRxiv - Neuroscience 2021Quote: ... Processed spinal cord slides were incubated with DAPI (made up in water) for 5 minutes in a hydration chamber at room temperature and a coverslip was mounted with vectoshield (Vector labs). Process slides were imaged on a Leica SP8 confocal microscope.
-
bioRxiv - Cell Biology 2021Quote: ... Tween 20 (TBST) for 5 min each and incubated at room temperature for an hour in secondary antibody-HRP (Vector Laboratories). Antibodies were then visualized by peroxidase substrate kit DAB (Vector Laboratories) ...
-
bioRxiv - Neuroscience 2022Quote: ... three times for 5-min and then blocked for 30-min with 30% normal goat serum (Vector Labs, cat# S-1000) and 1X PBS/Triton 0.3% ...
-
bioRxiv - Immunology 2020Quote: ... tissues were washed twice for 5 minutes in wash buffer and stained using the ImmPRESS universal (Anti-Mouse/Anti-Rabbit) kit (Vector labs).
-
bioRxiv - Cell Biology 2021Quote: ... cells were washed twice for 30 min with 10% formamide in 2X SSC before washing for 5 min with PBS and mounting of the coverslips using Vectashield (Vector Laboratories).
-
bioRxiv - Immunology 2021Quote: ... slides were washed 5 times with 1X PBS prior to being mounted with VectaShield medium containing DAPI (Vector lab, Burlingame, CA).
-
bioRxiv - Evolutionary Biology 2022Quote: ... The samples were washed three times in PBTx for 15 min followed by five 5-minute washes in PBS and mounted in Vectashield antifade mounting medium containing DAPI (Vector Laboratories). Samples were imaged on an Olympus FV3000 Confocal Laser Scanning Microscope and processed in Imaris.
-
bioRxiv - Microbiology 2022Quote: ... The membrane was washed 6 separate times in TBS-T for 5 min and then incubated with HRP-conjugated streptavidin (Vector Laboratories) for an additional hour ...
-
bioRxiv - Neuroscience 2023Quote: ... The CST was labeled using the Avidin Biotin Complex method (ABC) to detect the BDA by incubating in ABC (5 µL/mL A and B; PK-6100; Vector Laboratories) overnight at room temperature and co-labeled against GFAP (1:5000 ...
-
bioRxiv - Neuroscience 2023Quote: ... tissue sections were washed twice in PBS (5 min) and mounted using Vectashield without DAPI (Vector Laboratories Inc., Burlingame, CA, USA) for immediate visualization by microscopy.
-
bioRxiv - Cancer Biology 2023Quote: ... Ki-67 immunohistochemistry (IHC) was performed on 5 μm sections using a mouse on mouse immunodection kit (Vector Laboratories BMK-2202). Briefly ...
-
bioRxiv - Pathology 2023Quote: ... The sections were washed with PBST (3x, 5 min) and developed using a DAB substrate kit (Vector Laboratories, Burlingame, CA, USA). Counterstaining was performed with hematoxylin ...
-
bioRxiv - Biochemistry 2022Quote: ... Fixative was washed out with a continuous flow of 50 μl blocking buffer (5% normal goat serum S1000 in low-salt CSFxB; Vector Labs), then reactions were incubated in that buffer for 1 hour at 4°C ...
-
bioRxiv - Cell Biology 2022Quote: ... Organs were washed three times in PBS for 5 min per wash and mounted in Vectashield (Cat# H-1000, Vector Laboratories).
-
bioRxiv - Molecular Biology 2024Quote: ... Then the fixed specimens were washed thrice for 5 min in 1x phosphate buffered saline (1xPBS; pH 7.4) and mounted using Vectashield mounting medium (H-1000, Vector Laboratories, CA). FITC-albumin and 10 kD dextran specimens were imaged using a ZEISS LSM900 confocal microscope with ZEISS Zen acquisition software (blue edition ...
-
bioRxiv - Cell Biology 2024Quote: ... cells were stained with 0.5 μg/mL DAPI in PBS for 5 min prior to drying with ethanol and were mounted using Vectashield mounting medium without DAPI (Vector Laboratories). Images were acquired and analysed using a Leica fluorescence microscope ...
-
bioRxiv - Cell Biology 2019Quote: ... Agglutinin 1 (UEA1, Vector Labs), and EPCAM (9C4 ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... biotinylated (1/200; Vector Laboratories) for 2 hours ...
-
bioRxiv - Immunology 2023Quote: ... and UEA-1 (Vector Laboratories). Images were analyzed with a TCS SP8 confocal laser scanning microscope (Leica).
-
bioRxiv - Cancer Biology 2024Quote: ... IB4 (1:200, Vector Laboratories), anti-Ki67 (1:50 ...
-
bioRxiv - Cell Biology 2019Quote: ... oocytes were incubated with 4’,6-diamidino-2-phenylindole (DAPI) for 20 min and mounted onto glass slides using Vectashield (Vector Laboratories, Burlingame, CA, USA). Fluorescence was examined by confocal laser scanning microscopy (TCS SPE II ...
-
bioRxiv - Neuroscience 2021Quote: ... samples were washed 4-6 times over 2 hours in PBT at room temperature and mounted with VECTASHIELD Mounting Media (Vector Labs, Burlingame, CA, USA) using glass slides between two bridge glass coverslips ...
-
bioRxiv - Bioengineering 2021Quote: ... Mounting was conducted by incubating for 16 h at 4 °C with VECTASHIELD® PLUS Antifade Mounting Medium (H-1900; Vector Laboratories, Burlingame, CA). Extended information detailing primary antibody ...
-
bioRxiv - Animal Behavior and Cognition 2021Quote: ... each slide was coverslipped using a mounting medium that contained the nuclear stain 4’,6-diamidino-2-phenylindole (DAPI) (Vectashield; Vector Lab, Burlingame, CA, USA) and stored at −20°C until image acquisition occurred.
-
bioRxiv - Microbiology 2022Quote: ... The cells were washed twice with DPBS before the coverslips were mounted on to a glass slide with 4 μl Vectashield with DAPI (Vector Laboratories, cat# H-1200) and sealed with clear nail polish ...
-
bioRxiv - Neuroscience 2023Quote: ... The slices were mounted on glass slides with a mounting solution containing DAPI (4’,6-diamidino-2-phenylindole; Vectashield with DAPI, Vector Laboratories, Burlingame, CA, USA). Images were acquired using a slide scanning system (Panoramic Scan ...
-
bioRxiv - Pathology 2023Quote: ... The tissue sections were washed and mounted on microscope slides with VECTASHIELD Antifade Mounting Medium with 4′,6′[Diamidino[2[phenylindole (DAPI; H-1200, Vector Laboratories, Newark, CA, USA) to preserve fluorescence and nuclear staining ...
-
bioRxiv - Cancer Biology 2023Quote: ... Autofluorescence in tissue sections due to aldehyde fixation was removed by treatment with the TrueVIEW Autofluorescence Quenching Kit for 4 min (Vector Laboratories; SP-8400-15). Sections were counterstained with DAPI (ThermoFisher 62247 ...
-
bioRxiv - Developmental Biology 2023Quote: ... Nuclei were counterstained with 4’,6-diamidino-2-phenyl-indole (DAPI) diluted at 10 μg/ml in the mounting medium (Vectashield; Vector Laboratories, Newark, CA, USA). ImmPRESS® Polymer Detection Kits MP-7500 and MP-7405 were used according to the manufacturer’s protocol (Vector Laboratories).
-
bioRxiv - Evolutionary Biology 2023Quote: ... Chromosomes and nuclei were stained with 2 ng/µl DAPI (4’,6-diamidino-2-2phenylindole) in Vectashield antifade medium (Vector Laboratories, Burlingame, CA, USA). Preparations were analyzed with an Axiolmager M2 epifluorescent microscope (Carl Zeiss ...
-
bioRxiv - Microbiology 2022Quote: ... cells were stained at room temperature with 5 μg/mL fluorescein-conjugated wheat-germ agglutinin (WGA, Vector Laboratories catalog no. FL-1021) for 15 minutes to detect exposed chitin or 50 μg/mL fluorescein-conjugated concanavalin A (ConA ...
-
bioRxiv - Neuroscience 2021Quote: Free-floating parallel brain sections were washed with PBS + 0.5% Triton X-100 (PBS-T) and then blocked with 5% normal goat serum (NGS, #S1000, Vector laboratories, Burlingame, CA) in PBS-T for 1 h ...
-
bioRxiv - Neuroscience 2020Quote: ... The sections were incubated at 4 °C overnight with primary antibody diluted in PBS containing 0.3% Triton X-100 and 5% normal goat serum blocking solution (Vector Laboratories, #S-1000). Antibodies were as follows ...
-
Neuron-specific knockouts indicate the importance of network communication to Drosophila rhythmicitybioRxiv - Neuroscience 2019Quote: ... the brains were rinsed 5 × 10 mins with PBST and mounted on glass slides using Vectashield (Vector Laboratories INC., Burlingame, CA, USA) mounting medium.
-
bioRxiv - Cell Biology 2021Quote: ... membranes were washed 4 × 10min in TBS 0.1% Tween and incubated with one of the following peroxidase-conjugated secondary antibodies diluted in TBS 0.1% Tween supplemented with 5%BSA for 2h at room temperature: horse anti-mouse IgG(H+L) (Vector laboratories, PI-2000), goat anti-rabbit IgG(H+L ...