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Citations for Vector Labs :
701 - 750 of 763 citations for Recombinant Goat VEGFA Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... staining was 2% goat serumPrimary antibodies were left overnight in 4 degrees and followed by anti-rabbit secondary (Vector Laboratories Cat# BA-1000). Next ...
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bioRxiv - Neuroscience 2022Quote: ... nonspecific binding was blocked with a 1 h incubation in PBS-TX with 10% normal goat serum (NGS; S-1000, Vector Laboratories, Burlingame, USA) or normal donkey serum (NDS ...
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bioRxiv - Neuroscience 2023Quote: ... or the corresponding secondary antibodies: biotinylated goat anti-guinea pig antibody (1:100 dilution for GFP detection; Vector Laboratories, Cat# PK-4007; RRID:AB_2336816) and secondary antibody goat anti-mouse IgG coupled to 1.4 nm gold (1:100 dilution for TH detection ...
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bioRxiv - Neuroscience 2023Quote: ... USA) followed by secondary biotinylated antibody incubation (1:200, goat anti-rabbit IgG, Cat. No. BA-1000; Vector Labs Inc., Burlingame, CA, USA). Controls for nonspecific binding consisted in omitting the primary antibody (S Fig 5) ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue was then washed with 0.1 M TBS (5 × 5 min) and incubated in ImmPRESS HRP goat anti-rabbit IgG polymer detection kit reagent (Vector Laboratories, MP-74551) for 18 h at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... Alexa Fluor 488 goat anti-rabbit IgG or Alexa Fluor 546 goat anti-rabbit IgG conjugates for 2 hours and mounted in VECTASHIELD mounting medium with DAPI (Vector Laboratories, Burlingame, CA). Fluorescence images were taken using an inverted Nikon Eclipse TE2000-U microscope ...
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bioRxiv - Molecular Biology 2023Quote: ... non-specific binding sites were blocked with Tris-phosphate buffered saline (TPBS) supplemented with 10% normal goat serum (Vector Labs, Newark, CA, USA) and 3% bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2023Quote: ... fresh frozen brain slices on glass coverslips were incubated in PBS solution containing 10% heat inactivated goat serum (Vector Laboratories, Ref S-100) and 0.3% Triton X-100 for 20 min ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed three times in 0.1% Triton X-100 in TBS followed by 1 hr incubation with goat biotinylated anti-rabbit IgG secondary antibody (1:250, BA-1000, Vector Laboratories, RRID: AB_2313606) at room temperature and 30 min incubation with Vectastain Elite ABC reagent (PK-6100 ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were washed and incubated in the presence of biotinylated goat anti-rabbit IgG followed by Vectastain elite ABC reagent (1:600, #PK6101, Vector Laboratories, Burlingame, USA). Fos-like immunoreactive cells were visualized with the avidin-biotin peroxidase method using diaminobenzidine as a chromogen ...
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bioRxiv - Developmental Biology 2024Quote: ... Biotin-conjugated anti-goat or anti-rabbit secondary antibodies were each applied at 1:500 dilution and incubated with Vector elite Reagent (Vector Laboratories, CA, USA) for 45 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... Slides were washed again with PBS and blocked with 2.5% Normal Goat Serum for 20 minutes at room temperature (ImmPRESS Reagent, Vector Lab, cat#: MP-7451) and then incubated with the anti-rabbit ImmPRESS IgG peroxidase reagent (Vector Lab ...
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bioRxiv - Neuroscience 2020Quote: ... Tissue was rinsed 3 × 5 min in PBS followed by blocking in 20% normal goat serum (NGS; Vector Labs S-1000, Burlingame, CA, USA) in PBS for 1 hr at RT ...
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bioRxiv - Neuroscience 2020Quote: ... secondary antibodies (goat anti-rabbit, Vector Laboratories inc., PI-1000, 1:1,000 and biotinylated horse anti-mouse, Vector Laboratories inc., PK-4002, 1:200) were added to the sections for 150 min ...
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bioRxiv - Neuroscience 2022Quote: ... This was followed by an incubation with biotinylated goat anti-rabbit secondary antibody (1:200 in 3% NGS + 0.1% Triton X-100; Vector Labs, Burlingame, CA; Lot# 140893). Next ...
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bioRxiv - Neuroscience 2022Quote: ... sections were washed 5x in 0.1M PBS for 10 min per wash and incubated overnight at 4°C in secondary antibody (biotinylated goat anti-rabbit IgG; 1:500; Vector Laboratories, Inc, Burlingame, CA). The last day ...
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bioRxiv - Bioengineering 2022Quote: ... primary monoclonal rat anti mouse Mac1 antibodies (AbD Serotec, Raleigh, NC, USA) were used along with secondary biotinylated goat anti-rat antibodies (Vector Laboratories, Burlingame, CA, USA). Each midbrain section was viewed at low power (10x objective) ...
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bioRxiv - Immunology 2022Quote: ... Detection of bound antibodies was performed with biotinylated secondary goat anti-rabbit antibody and ABC-Kit using the peroxidase substrate method DABImmPact (all from Vector Laboratories, Burlingame, CA, USA).
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bioRxiv - Cell Biology 2022Quote: ... Endogenous peroxidase was blocked for 5 mins before incubation with 2.5% Normal Goat Serum (ImmPRESS Rat kit – Vector Laboratories, San Francisco Bay Area, USA) TRU for 20 mins ...
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bioRxiv - Neuroscience 2021Quote: ... followed by incubation overnight at 4 °C in secondary antibody diluted in block (1:500 goat anti-chicken Alexa 488, Life Technologies, A11039; 1:5000 biotinylated goat anti-rabbit, Vectastain Elite ABC HRP kit, PK-6101, Vector Laboratories, Burlingame, CA). Sections were washed several times in TBS with 0.3% Triton X-100 and 0.05% Tween 20 ...
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bioRxiv - Immunology 2022Quote: ... were incubated at 4°C overnight followed by washing and incubation with a secondary biotinylated goat anti-mouse IgG antibody (dilution: 1:200, Vector Laboratories, Burlingame, California, USA). For color development freshly prepared avidin-biotin-peroxidase complex (ABC ...
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bioRxiv - Cancer Biology 2022Quote: ... Sections were incubated with ImmPRESS® - HRP anti-goat IgG reagent for 1 hr and developed using ImmPACT® NovaRED or ImmPACT® DAB (Vector Laboratories). Images were captured with a Nikon 80i Eclipse microscope and processed with NIS-Elements Imaging Software.
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bioRxiv - Cell Biology 2019Quote: ... with bound antibodies being detected using anti-rabitt (for SP-D, TNFα, TSLP and CCSP) or anti-goat (for TLR4 and pEGFR) biotin-labeled antibodies (Vector Laboratories, Burlingame, CA, USA) in 1% PBS-BSA ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were then rinsed and incubated in secondary antibody solution for 24h at 4°C: 1:1000 rabbit anti-goat (Vector Laboratories, Burlington, ON, Canada) in 0.1M PBS ...
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bioRxiv - Cell Biology 2021Quote: ... or goat anti-mouse IgG Alexa Fluor 647 (Thermo, A32728) were diluted 1:200 in blocking buffer and 1% normal goat serum (Vector Laboratories, S-1000-20) and incubated for 2 hours at room temperature in a dark container ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated at room temperature for 1.5 hours in secondary antibody solution containing either biotinylated rabbit anti-goat IgG (Vector Laboratories, Burlingame, CA; PK-6105) or biotinylated goat anti-rabbit IgG (Vector Laboratories ...
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bioRxiv - Neuroscience 2023Quote: ... free-floating sections were first washed 3 × 10 min in 1x PBS and then incubated for 30 min in a blocking solution of 2% normal goat serum (S-1000-20; Vector Laboratories, Newark, CA, USA) and 0.1% Triton x-100 (T8787-50ML ...
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bioRxiv - Cancer Biology 2022Quote: ... Cleaved caspase 8 immunohistochemistry staining was performed using cleaved caspase 8 primary antibody (1:100; Cell Signalling Technology, cat. #9496) and goat anti-rabbit biotinylated secondary (1:200; Vector Laboratories, cat. BA-1000). Briefly ...
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bioRxiv - Genetics 2023Quote: ... slides were blocked for one hour in 10% goat serum and incubated for one hour at room temperature using a M.O.M kit (Vector Labs Cat# BMK-2202; Newark, CA).
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bioRxiv - Bioengineering 2023Quote: ... incubation with secondary antibodies (either anti-goat, anti-rabbit, or anti-mouse biotinylated secondary antibody, all from Vector Labs, California, USA, at 1:200), detection using a Vector Elite ABC kit (Vector) ...
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bioRxiv - Neuroscience 2023Quote: ... hBO sections were washed once with 1x PBS and blocked in blocking buffer containing 10% normal goat serum (NGS, Vector labs: S-1000-20), 0.1% BSA and 0.3% Triton-X in 1× PBS for one hour at RT ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Peroxidase-conjugated avidin and the secondary biotinylated antibodies rabbit anti-mouse IgG and rabbit fblanti-goat used in immunohistochemical studies were from VECTASTAIN® Elite ABC-Kit (Vector Laboratories, Burlingame, CA)
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bioRxiv - Genetics 2021Quote: ... biotin and protein blocking following Vector labs ABC elite staining kit (Vector labs, cat. number PK-6104) prior to incubation with monoclonal rat anti-MRP9 antibodies at 1:50 overnight at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Env protein was extracted from the culture supernatants using a Galanthus nivalis lectin (GNL) column (Vector Labs), whereas for all SApNPs ...
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bioRxiv - Neuroscience 2024Quote: ... Visualization of the target protein was achieved by adding 3,3’-diaminobenzidine (DAB) solution (Vector Laboratories #SK-4100), and the reaction was stopped by placing the sections in PBS ...
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bioRxiv - Microbiology 2022Quote: ... the secondary biotinylated goat anti-mouse or anti-rabbit antibody was applied for 30 minutes at room temperature (Vector Laboratories, Burlingame, CA, USA, 1:200). Slides were scanned using Hamamatsu S60 scanner ...
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bioRxiv - Neuroscience 2019Quote: ... sections were incubated for 1 hr at room temperature in a biotinylated solution (biotinylated goat anti-rabbit IgG; 1:200 dilution; Vector Laboratories; catalog no. BA-1000) before incubating in an avidin-biotin horseradish peroxidase solution for 2 hrs at room temperature (Vector Novoxastra Laboratories) ...
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bioRxiv - Microbiology 2022Quote: ... Slides were washed thrice with PBS to remove excess antibody and incubated with a rabbit anti-goat biotinylated IgG (Vector laboratories catalogue number BA-5000) for 10 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... The second primary was added to the sections overnight at 4°C then detected with a goat α-rabbit DyLight® 488 (DI1488, Vector Laboratories, 1:200) and mounted using VECTASHIELD® Antifade Mounting Medium with DAPI (H-1200-10 ...
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bioRxiv - Neuroscience 2023Quote: ... for the primary and Anti-glutamate receptor 1 (rabbit polyclonal) Goat Anti-rabbit HRP (Novus Biologicals Cat# NB7160, 1:250; Vector Laboratories, Burlingame, CA, USA) for the secondary antibodies ...
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bioRxiv - Biochemistry 2023Quote: ... Secondary rabbit anti-rat IgG and tertiary goat anti-rabbit IgG with horseradish peroxidase (HRP; ImmPRESS® HRP) was purchased from Vector Laboratories (Newark, CA, USA).
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bioRxiv - Cancer Biology 2021Quote: ... Antibody-protein interactions were visualized with Vectastain Elite ABC and DAB peroxidase substrate kits (Vector Laboratories, Burlingame, CA) and counterstained with nuclear fast red.
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Targeting MOG to skin macrophages prevents EAE in macaques through TGFβ-induced peripheral tolerancebioRxiv - Immunology 2019Quote: ... Incubation with primary antibodies was followed by a biotinylated goat-anti-rabbit antibody for 30 min followed by the avidin–biotin–peroxidase complex (Vectastain Elite ABC Kit, Vector Laboratories, PK 6100; Burlingame, CA, USA) for 30 min at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated with a rabbit polyclonal anti-syncollin antibody for 2 h at room temperature followed by an FITC-conjugated goat anti-rabbit secondary antibody and 4′,6-diamidino-2-phenylindole (DAPI, Vector Laboratories, Burlingame, CA; 1.5 μg.mL−1).
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bioRxiv - Neuroscience 2021Quote: ... sections were washed in PBST and incubated for 60 minutes at room temperature with a secondary biotinylated goat anti-rabbit antibody (Vector laboratories, Cat# BA-1000, lot# 2F0430) diluted 1:1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... and 5% goat serum) for 1 h at RT and then stained with EC specific biotinylated isolectin B4 (Vector lab, B-1205-.5, 1:100 dilution) overnight at 4 °C and followed by staining with FITC- streptavidin (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... followed by an ALP-coupled goat anti-mouse immunoglobulin G secondary antibody (ImmunoLogic, WellMed, MS Arnhem, Netherlands) and ImmPACT® Vector® Red Substrate (Vector Laboratories, Newark, CA, USA) visualization.
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bioRxiv - Cancer Biology 2022Quote: ... sections were incubated for 1 hr with species-specific Horseradish Peroxidase (HRP)-conjugated secondary antibodies (ImmPRESS HRP Goat Anti-Mouse or Rabbit IgG Polymer Detection Kit, Peroxidase, Vector Laboratories, Cat. #: MP-7452 and MP-7451) before developing positive staining with ImmPACT DAB Substrate Peroxidase (HRP ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... followed by treatment with secondary biotinylated rabbit anti-goat IgG or rabbit anti-mouse IgG (30 min, room temperature) and peroxidase-conjugated avidin (VECTASTAIN® Elite ABC-Kit; Vector Laboratories, Burlingame, CA; another 30 min). The chromogen 3-amino-9-ethylcarbazole (AEC Substrate Pack ...
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bioRxiv - Neuroscience 2023Quote: ... sections were rinsed with 0.1 M PB (4 × 5 min) and incubated in biotinylated goat anti-mouse IgG antibody (Vector Laboratories: 1/200 in 2% NGS in 0.1M PB) for 30 min ...