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Citations for Vector Labs :
601 - 650 of 746 citations for Recombinant Human CD28 protein Biotin labelled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Sections were then washed five times for ten minutes in PBS and then incubated in an avidin-biotin horseradish peroxidase complex (1:1000; ABC Vector Elite kit; Vector Laboratories) for 4 h at room temperature ...
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bioRxiv - Immunology 2021Quote: ... The red-brown antigen labelling was developed by application of avidin-biotin-peroxidase complex (ABC) solution (Vectastain ABC Kit, #PK 6100, Vector Laboratories), followed by exposure to 3-amino-9-ethylcarbazole substrate (AEC ...
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bioRxiv - Neuroscience 2019Quote: ... the tissue was incubated for 40 min in biotinylated secondary anti-rabbit antibody made in goat (1:200; Vector BA-1000) followed by incubation with the avidin–biotin complex solution (Vectastain ABC kit, Vector Laboratories). Immunoreactivity was visualized using standard DAB procedures ...
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bioRxiv - Cancer Biology 2020Quote: ... after washing sections were incubated with biotinylated secondary antibody at room temperature for 1 hour followed by incubation with ABC (Avidin Biotin complex) (Vector Labs) for 30 minutes ...
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bioRxiv - Developmental Biology 2021Quote: ... the slides were washed with PBS (pH 7.4) and submitted to the avidin-biotin-peroxidase solution (ABC Kit -PK -6100 Vector Laboratories®) for 45 minutes ...
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bioRxiv - Immunology 2020Quote: ... Slides were incubated with primary antibody for overnight and secondary antibody for 20 minutes and then the staining was amplified using avidin-biotin-peroxidase conjugate (ABC elite; Vector Laboratories) and visualized using 3 ...
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bioRxiv - Neuroscience 2022Quote: ... Immunohistochemical reactions on free-floating brain and spinal cord sections using the avidin-biotin peroxidase method (Vectastain, Vector Laboratories, Burlingame, CA) were used to identify rabies-infected neurons ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated with a solution of avidin-biotin-peroxidase complex (1:100; VECTASTAIN Elite ABC Standard Kit, Vector Laboratories) overnight ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then wash 3 times for ten minutes each in TBST and then incubated with prepared elite avidin biotin complex (ABC) reagent (Vector Laboratories) for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... free-floating sections were rinsed and bound antibodies were visualized by the Avidin-Biotin complex (ABC) amplification system (Vectastain ABC kit, Vector Laboratories) using 3,3′-diaminobenzidine tetrahydrochloride (DAB Substrate Kit ...
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bioRxiv - Neuroscience 2023Quote: ... followed by additional rinses and an overnight incubation in avidin biotin complex (Vectastain Elite ABC kit HRP, standard, cat#PK-6100, Vector Laboratories). The next day sections were rinsed in PBS and incubated in a solution containing 3’3’-diaminobezidine tetrahydrochloride hydrate (DAB ...
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bioRxiv - Neuroscience 2023Quote: ... and hybridized with 2 µg/ml digoxigenin (DIG)- and dinitrophenol (DNP)-labeled probes in a hybridization buffer containing the Biotin solution (Vector Laboratories) overnight at 60°C in a humidified chamber ...
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bioRxiv - Cancer Biology 2022Quote: ... Development of the biotinilated antibody signal was done by 1 h incubation with the Avidin/Biotin-HRP complex (Elite Vectastain ABComplex Kit, Vector Laboratories) following 1 h incubation with Streptavidin-647 (BioLegend) ...
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bioRxiv - Neuroscience 2023Quote: ... 1:1000) antibodies and biotin-conjugated anti-mouse secondary antibody (1:200) before applying the DAB peroxidase substrate kit (Vector laboratories) and imaging with a Nikon Eclipse 80i microscope ...
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bioRxiv - Systems Biology 2023Quote: ... Biotin-conjugated antibodies were prepared 100µg at a time using the ChromaLINK One-Shot Antibody Biotinylation Kit (Vector labs, Newark, USA).
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bioRxiv - Neuroscience 2023Quote: ... followed by incubation with a biotin-conjugated anti-rabbit or anti-mouse antibody (1:250, Vectastain Elite ABC kit, Vector laboratories). Sections were counterstained with Nissl (0.09% thionin ...
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bioRxiv - Neuroscience 2023Quote: ... The slices were washed with PBS (10 min, three times) and incubated with biotin-labeled goat anti-rabbit secondary antibody (1:500; Vector Labs) at room temperature for 90 min ...
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bioRxiv - Microbiology 2023Quote: ... infectious foci were visualized by incubation with Streptavidin Biotin Complex Peroxidase (Nacalai Tesque) for 30 min followed by incubation with VIP Peroxidase Substrate (Vector Laboratories) for 10 min.
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated with a biotin-conjugated anti-mouse secondary antibody (1:200) and revealed with the DAB peroxidase substrate kit (Vector laboratories) before imaging with a Nikon Eclipse 80i microscope ...
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bioRxiv - Neuroscience 2024Quote: ... Immunohistochemistry was performed on free-floating sections using a standard avidin-biotin (ABC Standard Elite kit, Vector Laboratories, Burlingame, CA, USA) method ...
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bioRxiv - Physiology 2024Quote: ... then rinsed several times in PB followed by incubation for two hours at room temperature with avidin–biotin–peroxidase (ABC; 1:250 in PB; VECTASTAIN Elite ABC kit PK6100, Vector Laboratories). The immunoreaction was visualized with 3,3-diaminobenzidine (DAB) ...
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bioRxiv - Cancer Biology 2024Quote: ... The IHC signal was amplified with a biotin-avidin-peroxidase system (ABC Elite Kit Vector) and visualized using diaminobenzidine (DAB Kit, Vector Laboratories). For immunofluorescence ...
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bioRxiv - Physiology 2023Quote: ... Then the sections were treated in avidin/biotin-peroxidase complex (Vectastain Elite ABC Elite 1:500, Vector Laboratories Inc, Burlingame, US) and the GnRH-immunoreactivity was developed in 0.05% DAB /0.005% H2O2 in 0.05 M Tris buffer ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then washed and incubated for 1.5 hours in avidin- biotin complex solution (1:200; Vectastain standard kit, Vector Laboratories, RRID:AB_2336819). Finally ...
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bioRxiv - Cancer Biology 2024Quote: ... Incubation with biotinylated antibodies slides followed by modified Elite avidin-biotin peroxidase (ABC) method (Vector Laboratories, Burlingame, CA, USA; pk-6100) for 30 minutes (RT) ...
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bioRxiv - Neuroscience 2021Quote: ... goat anti rat (3052-08)) for 1 hour at room temperature and labelled structures were visualized using the ABC Vectastain kit (Vector laboratories, PK-6100). Finally ...
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bioRxiv - Microbiology 2019Quote: ... and holdfasts were labelled using 50 µl of fluorescent Alexa Fluor (AF488 or AF594) conjugated lectins (Molecular Probes or Vector Labs, Table 2) at a final concentration of 0.5 µg/ml ...
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bioRxiv - Microbiology 2021Quote: ... while sections labelled for CD3 were incubated with anti-mouse biotinylated secondary antibody (catalogue number BA-2000-1.5 Vector Laboratories, California, United States) at 1:50 with 1% BSA and thereafter incubated with Vectastain Elite ABC reagent (Vectastain Elite ABC Kit ...
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bioRxiv - Developmental Biology 2022Quote: ... we first detected the DIG-labelled nkx2.4b riboprobe with anti-DIG antibody (1:6000) and BM purple staining in 28 hpf embryos and the DNP-labelled tg riboprobe with anti-DNP antibody (Vector Laboratories, 1:500) and NBT/BCIP (Roche ...
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bioRxiv - Cell Biology 2020Quote: ... was carried out according to manufacturer’s protocol after fixation of organoids with 0.1% paraformaldehyde in PBS for 1 hour at room temperature followed by blocking with Avidin/Biotin (Vector Labs SP-2001). Biotin-Maackia Amurensis lectin was labeled with streptavidin-PE conjugate (Thermo Fisher SA10041 ...
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bioRxiv - Developmental Biology 2020Quote: ... immunohistochemistry was carried out using heat-induced epitope retrieval buffers (A. Menarini) and Vectastain avidin-biotin-HRP reagents (Vector Lab PK-6100). Anti-BAP1 antibody (Cell signalling ...
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bioRxiv - Neuroscience 2021Quote: ... signal amplification after species-specific secondary biotinylated antibody incubation of two hours was done using an avidin/biotin-based peroxidase system (PK6100, ABC Vectastain Elite kit, Vector Laboratories, UK). Chromogen development was sequential with double labelling ...
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Obesity alters mobility and adult neurogenesis, but not hippocampal dependent learning in ob/ob micebioRxiv - Neuroscience 2019Quote: ... Rinsing with PBS was followed by incubation in a solution containing biotin-conjugated horse anti-goat IgG (1:200 (Vector Laboratories, USA) in 3 % horse serum + 0.1 % Triton X-100 in PBS ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were incubated with a solution of avidin-biotin-peroxidase complex (1:100; VECTASTAIN Elite ABC Standard Kit, Vector Laboratories, PK-6100) overnight at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Another PBS/TWEEN washing battery was performed and sections were then incubated in an avidin-biotin-peroxidase complex (ABC kit, Vector Laboratories®) for 2 h ...
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bioRxiv - Neuroscience 2020Quote: ... sections were washed in PBS with 0.1% Triton X-100 three times then incubated overnight in avidin-biotin-peroxidase complex (Vector Laboratories, PK-6100) in PBS with 0.1% Triton X-100 at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated for 2 hours at room temperature with avidin–biotin–peroxidase (ABC; 1:250 in PB; VECTASTAIN Elite ABC kit, Vector Laboratories, PK6100). The immunoreaction was visualized with 3,3-diaminobenzidine (DAB) ...
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bioRxiv - Neuroscience 2022Quote: ... followed by a 1h incubation at room temperature with a 1:250 dilution of an avidin-biotin complex solution (ABC Vectastain kit, Vector Laboratories®). Revelation was performed using the DAB Peroxidase Substrate Kit (DAB SK4100 kit ...
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bioRxiv - Neuroscience 2021Quote: ... After inactivation of endogenous peroxidases and blocking with 3% H2O2 the tissue was treated with Avidin/Biotin Blocking Kit (SP-2002 Vector Labs, USA) followed by an incubation overnight at 4°C with antiDsRed rabbit polyclonal primary antibody (1:1000 ...
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bioRxiv - Physiology 2021Quote: ... the sections were immunostained by the avidin–biotin peroxidase method using the Vectastain Elite Kit (PK-6102, Vector Laboratories, Burlingame, CA, USA) with 3–3-diaminobenzidine and hydrogen peroxide as the chromogen ...
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bioRxiv - Cancer Biology 2020Quote: ... were used for immunohistochemistry (IHC) and immunofluorescence staining in combination with the avidin-biotin-peroxidase method (Vectastain, Vector laboratories, Burlingame, CA, US) or Alexa Fluor-488-conjugated secondary antibody ...
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bioRxiv - Physiology 2020Quote: ... sections were blocked for endogenous avidin and biotin activity and incubated with a biotinylated anti-rabbit IgG secondary antibody (0.5%, Vector Laboratories, Burlingame, CA) for 30 min at RT ...
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bioRxiv - Genetics 2020Quote: ... Slides were washed in PBS and incubated for 1 h in 1:400 diluted biotin-conjugated secondary antibodies (Vector Laboratories, Burlingame, CA) for IHC and 1:400 Alexa 488 or Alexa 594 (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... incubated with avidin-biotin complex kit (ThermoFischer Scientific, 32020) for 2 hrs and visualized with a 3,3’-diaminobenzidine kit (Vectastain, Vector Labs, SK-4100). Sections were then counterstained with cresyl violet for Nissl labeling.
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Serotonin, Etonogestrel and breathing activity in murine Congenital Central Hypoventilation SyndromebioRxiv - Physiology 2022Quote: ... and finally an avidin-biotin-peroxidase complex (ABC kit standard, PK-6100, Vectastain, Vector Laboratories, Burlingame, CA, USA; 1:250; 1 h). Peroxidase activity was detected using a solution containing 0.02 % 3,3’-diaminobenzidine tetrahydrochloride ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated in an avidin-biotin-peroxidase complex kit (Vectastain Elite ABC Kit, Vector Laboratories, Burlingame, CA, catalog no. PK-6100). Immunoreactivity was visualized by incubation in 0.05% 3’ ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and blocked with 10% goat or rabbit serum in PBS for 60 min and with Avidin/Biotin Blocking Kit (Vector Laboratories, Inc.) for 15 min ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by blocking of nonspecific binding sites using normal goat serum (10%) and an avidin/biotin blocking kit (Vector Laboratories, Burlingame, CA). Sections were incubated with phospho-SMAD2/3 (Cell Signaling Technology ...
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bioRxiv - Systems Biology 2024Quote: ... Sections were rinsed once more in 0.1% Triton-X in TBS then incubated in an avidin-biotin complex (PK-6100; Vectastain ABC Elite kit, Vector Laboratories, Newark, CA) for 1 hour ...
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bioRxiv - Biochemistry 2024Quote: ... The sections were then incubated for 45 min with the avidin-biotin-peroxidase complex (PK-6100, ABC Vectastain; Vector Laboratories, Burlingame, USA). Immunohistochemical reactions were visualized by incubating the sections with 0.05% 3,3-diaminobenzidine (DAB ...