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Citations for Vector Labs :
601 - 650 of 764 citations for IL 4 Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... Secondary antibodies were incubated with the sections for one hour before washing using PBS and mounting using Vectashield Antifade Mounting Medium with 4’,6-diamidino-2-phenylindole (DAPI) (Vector Laboratories, Burlingame, CA).
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bioRxiv - Neuroscience 2022Quote: ... Cells incubated with MitoSOX™ were then fixed with 3.7% paraformaldehyde and applied with an anti-fading mounting medium with 4′,6-diamidino-2-phenylindole (DAPI; H-1200, Vector Laboratories, Burlingame, CA). Live or fixed cell images were captured at an excitation/emission (in nm ...
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bioRxiv - Neuroscience 2023Quote: ... slices were incubated overnight at 4°C with a solution containing biotinylated Wisteria floribunda Lectin (WFA, B-1355-2, Vector Laboratories, 1:200) and 3% BSA in PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... staining was 2% goat serumPrimary antibodies were left overnight in 4 degrees and followed by anti-rabbit secondary (Vector Laboratories Cat# BA-1000). Next ...
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bioRxiv - Neuroscience 2023Quote: ... for 40 seconds and sections were finally coverslipped with Vectashield mounting medium containing 4′,6-diamidino-2-phenylindole (DAPI) to label the nucleus (Vector Laboratories, H-1800). Slides were kept at 4 °C until imaging ...
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bioRxiv - Physiology 2023Quote: ... the cells were fixed with 4% PFA at 4 ℃ for 20 minutes in the dark and mounted using VECTASHIELD® HardSet™ Antifade Mounting Medium (#H-1400-10, Vector Laboratories). Cell nuclei were stained with DAPI ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated overnight at 4 °C with STL-FITC (dilution 1:750, solanum tuberosum lectin-fluorescein isothiocyanate, endothelial marker, Vector Laboratories FL-1161) and goat anti-IBA1 (dilution 1:1500 ...
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bioRxiv - Cancer Biology 2022Quote: ... The sections were washed with PBS and mounted using VECTASHIELD® Vibrance™ mounting medium with 4′,6-diamidino-2-phenylindole (DAPI, Vector Laboratories). Table S6 lists the primary and secondary antibody dilutions for the organ tissues ...
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bioRxiv - Developmental Biology 2023Quote: ... in 1% NDS in PBS/MT at 4°C for 24 hr and subjected to peroxidase-conjugated streptavidin treatment in blocking solution (1:500; PK6200, Vector Laboratories, Burlingame, CA) at 4°C for 24 hr after rinsing in PBS/MT on ice (1 hr x 5) ...
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bioRxiv - Physiology 2023Quote: ... the sections were mounted with hard-set DAPI (4′, 6-diamidino-2-phenylindole –hard set; Cat# H-1500, Vector Laboratories Ltd., Peterborough, UK). The slides were left overnight in the dark at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... melanogaster larval imaginal discs were dissected in PBS and fixed in 4% paraformaldehyde and either mounted directly in VectaShield Mounting Medium (Vector Laboratories, H-1000) or first permeabilised with PBST (PBS with 0.3% Triton X-100 ...
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bioRxiv - Physiology 2023Quote: ... The coverslips were washed in PBS before being mounted onto a microscope slide with 4’,6-diamidino-2-phenylindole (DAPI) (Vectashield, Vector Laboratories, Burlingame, CA) mounting medium.
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bioRxiv - Cancer Biology 2023Quote: ... Slides were incubated with primary antibodies overnight at 4°C and with secondary antibodies for 1 hr at room temperature (RT) before ABC-HRP (Vector Laboratories #PK-7100) treatment and DAB (Vector Laboratories #sk-4105 ...
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bioRxiv - Cell Biology 2020Quote: ... membranes were further probed with HRP-conjugated anti-rabbit and anti-mouse secondary antibodies (Vector Laboratories# PI-1000, PI-2000). Immunoblots were developed using either Pierce ECL Western Blotting Substrate (ThermoFisher Scientific# 32106 ...
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bioRxiv - Molecular Biology 2019Quote: ... the sections were incubated with biotinylated anti-mouse or anti-goat secondary antibodies (1:750, Vector Laboratories, Burlingame, CA, USA) diluted in KPBS containing 1% BSA and 0.02% Triton X-100 for 1.5 h at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... was revealed by incubation with a biotinylated mouse secondary antibody (1:200) and VECTASTAIN Elite-ABC kit reaction (Vector Laboratories) with the TSA-Plus Fluorescein System NEL741001KT (Perkin-Elmer Inc.) ...
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bioRxiv - Neuroscience 2022Quote: ... Then slices were incubated with biotinylated goat anti-mouse IgG antibody (BA-9200, Vector Laboratories, Burlingame, CA, USA; 1: 500) and immunoreactivity revealed with Avidin-peroxidase conjugate (Vectastain ABC Elite ...
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bioRxiv - Genetics 2022Quote: ... Block solution was replaced with ImmPress-HRP Horse anti-rabbit/mouse secondary antibody (Vector Laboratories, MP-7401/ MP-7402-15) and incubated for 30 minutes at room temperature followed by 2X 5 minute rinses with PBS ...
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bioRxiv - Neuroscience 2020Quote: ... blots were washed and incubated with an HRP anti-mouse secondary antibody (1:10,000, Lot#: X0328; Vector Labs, Burlingame, CA). Blots were then developed in chemiluminescent substrate (ECL-Prime ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then processed with a secondary biotinylated horse anti-mouse IgG antibody (1:200, Vector Laboratories, Burlingame, CA, USA). They were then incubated for 1h in an avidin-biotin peroxidase complex (Vectastain ABC Elite PK6100 ...
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bioRxiv - Neuroscience 2021Quote: ... membranes were incubated in horseradish peroxidase secondary antibodies (anti-mouse, 1:10,000, Jackson ImmunoResearch; anti-rabbit, 1:10,000, Vector Labs) for 1 hr ...
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bioRxiv - Neuroscience 2022Quote: ... Cat# 111-035-144] and Peroxidase-conjugated horse anti-mouse IgG (H+L) antibody [(1:5,000) (Vector laboratories, PI-2000)] ...
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bioRxiv - Neuroscience 2019Quote: ... in 0.3% Triton X-100 PBS (PBST) was performed before overnight incubation with biotinylated anti mouse IgG (1:250 in in 0.3% PBST, Vector Laboratories) at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... cells were permeabilized by 0.1% saponin (in FACS-B with 1% normal mouse serum) with streptavidin added (Streptavidin/Biotin Blocking Kit, Vector Laboratories) to block endogenous biotin (permeabilizing and blocking solution) ...
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bioRxiv - Immunology 2019Quote: ... all slides were incubated with ImmPRESS™ HRP Reagent Kit Universal anti-mouse/rabbit IgG (Vector Laboratories, Burlingame, CA, USA) for 30 minutes ...
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bioRxiv - Pathology 2021Quote: ... Then slices were incubated with biotinylated goat anti-mouse IgG antibody (BA-9200, Vector Laboratories, Burlingame, CA, USA; 1: 500). Avidin-peroxidase conjugate revealed immunoreactivity (Vectastain ABC Elite ...
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bioRxiv - Pathology 2021Quote: ... tissues were washed twice with 1 mL wash buffer and the antigen:antibody complex detected with the ImmPRESS universal (Anti-Mouse/Anti-Rabbit) kit (Vector labs). Chromogenic solution (DAB ...
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bioRxiv - Molecular Biology 2021Quote: ... Sections were then incubated in biotinylated α mouse (6E10) or rabbit (AT8) secondary antibody (1:200; Vector Labs, Burlingame, CA) for 2 hrs at room temperature followed by Vectastain Elite ABC reagent (Vector Labs ...
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bioRxiv - Neuroscience 2022Quote: ... 2.5% horse normal serum from the ImmPRESS reagent kit Anti-mouse IgG/Anti-rabbit IgG (Vector Laboratories, Burlingame, CA, USA) was used for 30 min (Table 1) ...
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bioRxiv - Neuroscience 2022Quote: ... followed by incubation of the sections in biotinylated anti-rabbit/mouse secondary antibody (1:1,000 dilution, Vector Laboratories, Burlingame, CA) and then in avidin-biotin-peroxidase complex (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... The tissue was washed by PBS-TX (0.3% Triton X-100) and then incubated with 1:1000 dilution of biotinylated anti-mouse antibody (Vector Labs) with agitation ...
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bioRxiv - Neuroscience 2022Quote: ... Cat# 111-035-144] and Peroxidase-conjugated horse anti-mouse IgG (H+L) antibody [(1:5,000) (Vector laboratories, PI-2000)].
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bioRxiv - Pathology 2022Quote: ... Membranes were probed with a horseradish peroxidase-conjugated secondary antibody (1:5000; anti-rabbit IgG or 1:20,000; anti-mouse IgG, Vector Laboratories) in 5% not-fat milk powder in TBST (1h ...
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bioRxiv - Neuroscience 2022Quote: ... The sections were then incubated for 1 h at room temperature with biotin-conjugated goat anti-mouse secondary antibody (Cat#BA-9200-1.5, Vector Laboratories) at 1:200 dilution ...
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bioRxiv - Neuroscience 2023Quote: ... 10min each) followed by incubation in a secondary antibody solution (1:3000, peroxidase labeled anti-mouse, PI-2000, Vector laboratories). This was followed by rinsing in TBST ...
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bioRxiv - Neuroscience 2024Quote: ... washed and incubated in horse anti-mouse biotinylated secondary (1:200 in 2% BSA and 0.1% Triton {TBS]; Vector Laboratories) for 180 min prior to washing and development in Streptavidin-tertiary reagent (1:500 in 2% BSA and 0.1% Triton [TBS] ...
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bioRxiv - Cell Biology 2019Quote: ... oocytes were incubated with 4’,6-diamidino-2-phenylindole (DAPI) for 20 min and mounted onto glass slides using Vectashield (Vector Laboratories, Burlingame, CA, USA). Fluorescence was examined by confocal laser scanning microscopy (TCS SPE II ...
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bioRxiv - Neuroscience 2021Quote: ... The histological sections were washed three times (10 min/each) with PBS, counterstained with 4’, 6-diamidino-2-phenylindole (DAPI, 1:16000) and mounted with Vecta-Shield (H1000, Vector Laboratories, Burlingame, CA, USA) (Varman et al. ...
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bioRxiv - Neuroscience 2021Quote: ... samples were washed 4-6 times over 2 hours in PBT at room temperature and mounted with VECTASHIELD Mounting Media (Vector Labs, Burlingame, CA, USA) using glass slides between two bridge glass coverslips ...
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bioRxiv - Bioengineering 2021Quote: ... Mounting was conducted by incubating for 16 h at 4 °C with VECTASHIELD® PLUS Antifade Mounting Medium (H-1900; Vector Laboratories, Burlingame, CA). Extended information detailing primary antibody ...
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bioRxiv - Neuroscience 2022Quote: ... sections were washed 5x in 0.1M PBS for 10 min per wash and incubated overnight at 4°C in secondary antibody (biotinylated goat anti-rabbit IgG; 1:500; Vector Laboratories, Inc, Burlingame, CA). The last day ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... each slide was coverslipped using a mounting medium that contained the nuclear stain 4’,6-diamidino-2-phenylindole (DAPI) (Vectashield; Vector Lab, Burlingame, CA, USA) and stored at −20°C until image acquisition occurred.
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bioRxiv - Neuroscience 2022Quote: ... slices were rinsed again 4 times for 5 minutes in PBS and revealed using a DAB substrate kit (Vector Laboratories Cat# SK-4100, RRID:AB_2336382). Stained slices (at least 5 per region ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were then rinsed and incubated in secondary antibody solution for 24h at 4°C: 1:1000 rabbit anti-goat (Vector Laboratories, Burlington, ON, Canada) in 0.1M PBS ...
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bioRxiv - Microbiology 2022Quote: ... The cells were washed twice with DPBS before the coverslips were mounted on to a glass slide with 4 μl Vectashield with DAPI (Vector Laboratories, cat# H-1200) and sealed with clear nail polish ...
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bioRxiv - Neuroscience 2023Quote: ... The slices were mounted on glass slides with a mounting solution containing DAPI (4’,6-diamidino-2-phenylindole; Vectashield with DAPI, Vector Laboratories, Burlingame, CA, USA). Images were acquired using a slide scanning system (Panoramic Scan ...
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bioRxiv - Pathology 2023Quote: ... The tissue sections were washed and mounted on microscope slides with VECTASHIELD Antifade Mounting Medium with 4′,6′[Diamidino[2[phenylindole (DAPI; H-1200, Vector Laboratories, Newark, CA, USA) to preserve fluorescence and nuclear staining ...
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bioRxiv - Microbiology 2023Quote: ... the slides were stained with 4’,6-diamidino-2-phenylindole (DAPI) and mounted in a 4:1 mixture of Citifluor (Citifluor Ltd, London, UK) and Vecta Shield (Vector Laboratories, Inc., Burlingame, CA), covered with glass coverslips ...
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bioRxiv - Cancer Biology 2023Quote: ... Autofluorescence in tissue sections due to aldehyde fixation was removed by treatment with the TrueVIEW Autofluorescence Quenching Kit for 4 min (Vector Laboratories; SP-8400-15). Sections were counterstained with DAPI (ThermoFisher 62247 ...
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bioRxiv - Developmental Biology 2023Quote: ... Nuclei were counterstained with 4’,6-diamidino-2-phenyl-indole (DAPI) diluted at 10 μg/ml in the mounting medium (Vectashield; Vector Laboratories, Newark, CA, USA). ImmPRESS® Polymer Detection Kits MP-7500 and MP-7405 were used according to the manufacturer’s protocol (Vector Laboratories).