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Citations for Vector Labs :
601 - 650 of 801 citations for 5 Nitrobenzothiazol 2 thiol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Coverslips were mounted with Vectashield Vibrance antifade mounting medium containing 4′,6-diamidino-2-phenylindole (DAPI) (H-1800, Vector Laboratories) for immunocytochemistry with p62 or ...
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bioRxiv - Neuroscience 2024Quote: ... full-thickness colon segments of ∼1cm in length were blocked for 2 hrs in 1.5ml tubes containing 1 drop of Mouse-on-Mouse blocking reagent (MOM; Vector Labs) in 1ml of PBS with 0.5% Triton-X 100 ...
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bioRxiv - Developmental Biology 2024Quote: ... then mounted onto microscope slides with Vectashield Plus Antifade Mounting Medium containing 4’,6’-diamidino-2-phenylindole (DAPI, Vector Laboratories). Slides were imaged with a Leica TCS Sp5 confocal system with 40x or 63x objectives (Leica Microsystems ...
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bioRxiv - Cell Biology 2020Quote: ... Sections were incubated at room temperature with 3% H2O2 for 10 minutes to quench endogenous peroxidase activity and blocked by 5% normal goat serum (Vector Laboratories# S-1000) in TBST (TBS containing 0.1% Tween® 20 ...
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bioRxiv - Neuroscience 2020Quote: ... Endogenous peroxidase activity and non-specific binding were blocked by incubation with 3% hydrogen peroxide in methanol for 15 minutes followed by 5% normal goat serum (Vector Laboratories, CA, USA) with 0.1% TWEEN-20 in PBS for 1 hour ...
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bioRxiv - Neuroscience 2021Quote: ... incubated in 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked in 5% normal goat serum (NGS) (Vector Labs, Burlingame, CA) with 0.1% Triton X-100 ...
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bioRxiv - Synthetic Biology 2021Quote: ... blocked in 5% bovine serum albumin for 30 min and probed for 1 h with fluorescein-labeled SBA (Vector Laboratories, catalog # FL-1011) and Alexa Fluor 647® (AF647)-conjugated anti-His antibody (R&D Systems ...
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bioRxiv - Cell Biology 2021Quote: ... the retinal sections were washed with PBS three times for 5 min each and then mounted with Vectashield Mounting Medium (Vector Laboratories, Burlingame, CA). The images were acquired with a BZ-X810 Keyence microscope (Keyence ...
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bioRxiv - Physiology 2021Quote: ... Sections were incubated at room temperature with 3% (v/v) H2O2 for 10 min to quench endogenous peroxidase activity and blocked by 5% (v/v) normal goat serum (Vector Laboratories, #S-1000) in PBS for 1 hour ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were rinsed again in PBS and then incubated for 1 hr in avidin-biotin complex (4.5 μL each of A and B reagents/ml PBS with 0.4% Triton-X 100, ABC Elite Kit, Vector Laboratories, Burlingame, CA, USA). After rinsing in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were rinsed again in PBS and then incubated for 1 hr in avidin-biotin complex (4.5 μL each of A and B reagents/ml PBS with 0.4% Triton-X 100, ABC Elite Kit, Vector Laboratories, Burlingame, CA, USA). After rinsing in PBS ...
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Mechanotransduction-dependent control of stereocilia dimensions and row identity in inner hair cellsbioRxiv - Cell Biology 2019Quote: ... Organs were washed three time in PBS for 5 min per wash and then mounted in Vectashield (Cat#H-1000, Vector Laboratories, Burlingame, CA).
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bioRxiv - Neuroscience 2020Quote: ... Another set of washes in PBS (3x–5 min each) were performed and then the slides were mounted using Vectashield with DAPI (Vector Labs, H-1200).
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bioRxiv - Immunology 2020Quote: ... 3 times with PBTD and counterstaining with DAPI in 5% NGS blocking solution took place after the first wash. Coverslips (No. 1) were mounted using Vectashield (Vector Laboratories, Burlingame, California) and sealed with nail polish ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then rinsed in PBS (5 x 5 min) followed by a 1 hour incubation in biotinylated goat anti-rabbit secondary (1:500 in PBS, Vector Laboratories, BA-1000). Sections were rinsed in PBS (5 x 5 min ...
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bioRxiv - Molecular Biology 2022Quote: ... ATAC-see samples were incubated for 1 hour at room temperature protected from light with blocking buffer (1X PBS, 0.1% TX-100 and 5% normal goat serum (Vector Laboratories, S-1000-20). ds/ssDNA mouse monoclonal IgM antibody clone AC-30-10 (PROGEN ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed with PBS-TX (0.5% Triton X-100 in PBS) 6 x 20 min then blocked in 5% normal goat serum (NGS; Vector Laboratories, Burlingame, CA, USA) for 1 h before being incubated on a shaker overnight with primary antibodies (Table 1 ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were again rinsed five times in PBS and then incubated for 1 hr in avidin-biotin complex (4.5 μL each of A and B reagents/ml PBS with 0.4% Triton-X 100, ABC Elite Kit, Vector Laboratories, Burlingame, CA, USA). After rinsing in PBS ...
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bioRxiv - Neuroscience 2024Quote: ... slides were rinsed three times for 5 minutes with 0.4% Triton-X-100 TBS and mounted using VectaShield® Hardset ™ Antifade Mounting Medium with DAPI (Vector Laboratories, California). Slides were then left alone to set for at least 48 hours at 4° C and imaged on a Leica DMi8 Confocal Microscope.
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bioRxiv - Cancer Biology 2023Quote: ... Cell nuclei were stained with spectral DAPI in PBS for 5 min and the slides were mounted with Hard-set Vectashield mounting medium (Vector Laboratories, Burlingame, CA). Mounted slides were allowed to harden prior to scanning.
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... followed by washing 3x in PBS for 5 minutes before 60-minute incubation with a Streptavidin-conjugated Dylight 488 secondary (1:200, Vector Laboratories, SA-5488). After secondary ...
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bioRxiv - Bioengineering 2023Quote: ... Slides were washed with 1X TBS twice again for 5 minutes each and Vectashield Mounting Media (H-1200; Vector Laboratories, Newark, CA, USA) was added before coverslipping ...
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bioRxiv - Pathology 2023Quote: ... Tissue sections (5 mm) from each block were cut in a cryostat at −21 C and mounted onto Vectabond-coated slides (Vector Laboratories, Peterborough, UK), fixed in cold acetone for 10 minutes and incubated with an anti PECAM-1 rat primary antibody (BD Biosciences ...
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bioRxiv - Neuroscience 2023Quote: ... The intracellular single unit recordings were performed on the L2/L3 DRGs using the quartz microelectrode containing 5% Neurobiotin (Vector Laboratories, Burlingame, CA) in 1M potassium acetate ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Haematoxylin and Eosin (H&E) staining was performed on 5-μm sections that were prepared and stained using kit (Vector Laboratories, CA, USA) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... We washed slides twice for 5 minutes in RNase-free 1X PBS and marked the sample perimeter of each slide with an ImmEdge pen (Vector Laboratories #H-4000) in between both washes ...
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bioRxiv - Cell Biology 2023Quote: ... slides were washed in 3 x 5 min in TBST and mounted with Vectashield® mounting medium (Cat. No. H-1000; Vector Laboratories, USA).
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bioRxiv - Molecular Biology 2024Quote: ... Cells were washed with PBS and either stained for 5 min with 1 µg/ml DAPI (for chamber slides) or mounted using DAPI-containing Vectashield (Vector Laboratories; for coverslips). An Olympus IX70 inverted microscope with a UPlanApo 100X/1.35 oil iris objective was used for image acquisition with Micro-Manager software (v2.0) ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were washed 3 times with cold PBS and resuspended in Vectashield mounting medium with 4’,6-diamidino-2-phenylindole (DAPI) (Vector Laboratories). Cells were imaged at 100X magnification using a Leica DM IRBE microscope ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were washed with PBS for 3 times and mounted with VECTASHIELD antifade mounting medium with 4′,6-diamidino-2-phenylindole (DAPI, H-1200; Vector Laboratories). Images were taken by SP8 confocal microscopy (Leica Microsystems) ...
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bioRxiv - Genetics 2021Quote: ... Petals were removed from the buds and transferred to a microscope slide with 10ul of Vectashield Mounting Media containing 4’,6-Diamidino-2-Phenylindole (DAPI) (1.5ug/mL) (Vector Laboratories, Canada). A 22×22 mm coverslip was added and mild pressure was applied to the coverslip ...
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bioRxiv - Microbiology 2020Quote: ... The slides were allowed to air dry before placing a drop of Vectashield mounting medium containing DAPI (4’,6-Diamidino-2-phenylindole dihydrochloride) (Vector Laboratories) on them ...
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bioRxiv - Molecular Biology 2021Quote: ... Slides were finally mounted with Vectashield Mounting Media with 4,6-diamidino-2-phenylindole as nuclear counterstain (Vector Laboratories, Cat# H-1200). Stainings were digitalized employing the Olympus BX61VS microscope and scanner software VS-ASW-S6 (Olympus ...
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bioRxiv - Genomics 2021Quote: ... Flies were dissected 10 to 40 days pi and organs viewed by fluorescence microscopy and imaged live or fixed in 2% paraformaldehyde and stained with DAPI in VECTASHIELD mounting medium (Vector Laboratories).
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bioRxiv - Neuroscience 2020Quote: ... Following 3 washes in PBS-T they were incubated for 2 hr at RT with the appropriate biotinylated secondary antibody (Vector Laboratories) diluted 1:500 in PBS-T ...
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bioRxiv - Neuroscience 2021Quote: ... unexpanded sections were washed in PBS-T and mounted under Vectashield fluorescence media with 4’,6-diamidino-2-phenylindole (DAPI) (Vector Laboratories).
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bioRxiv - Cancer Biology 2020Quote: ... Cells were postfixed with 10 % NBF for 2-3 min before mounting on a clean glass slide using Vectashield antifade mounting media (Vector Laboratories). Image acquisition was performed on the LSM 700 laser confocal microscope (Zeiss) ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were washed and transferred to donkey anti-rabbit secondary antibody (1:500; Jackson Immuno) for 2 hr before detection with ABC (Vectastain ABC Kit, Vector Laboratories) and visualization with DAB substrate (Vector Laboratories) ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were then incubated overnight at 4°C with their respective primary antibodies (1%–2% BSA in TBST) followed by horseradish peroxidase IgG (Vector Laboratories)-conjugated secondary antibody (anti-rabbit and anti-mouse ...
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Role of the Topoisomerase IIα Chromatin Tether domain in Nucleosome Binding & Chromosome SegregationbioRxiv - Cell Biology 2021Quote: ... and then treated with secondary antibodies for 1h before being washed and mounted onto slide glasses with VECTASHIELD Antifade Mounting Medium containing 4’,6-diamidino-2-phenylindole (#H-1200, Vector Laboratory) and sealed using nail polish.
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bioRxiv - Biochemistry 2022Quote: ... 0.02% Triton X-100 and mounted onto slides with Vectashield anti-fade mounting media containing 4’,6-diamindino-2-pheylindole (DAPI, Vector Laboratories). Slides were imaged on a Zeiss Cell Observer widefield fluorescence microscope and analysed using ImageJ.
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bioRxiv - Molecular Biology 2022Quote: ... slides were dried and counterstained with 1.5μg/mL 4′,6-diamidino-2-phenylindole (DAPI) and mounted in Vectashield antifade mounting medium with DAPI (Vector Laboratories, Piedmont, Italy) before being sealed with a coverslip and nail varnish.
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bioRxiv - Neuroscience 2022Quote: ... incubated in appropriate secondary antibody solutions for 2 hours (ChAT (1:200): rabbit anti-goat IgG (biotinylated; Vector Laboratories, BA-5000); PV (1:200) ...
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bioRxiv - Immunology 2022Quote: Coverslips were mounted on slides with VECTASHIELD Antifade Mounting Medium containing 4’,6-diamidino-2-phenylindole (DAPI) (Vector Laboratories; H-1200). Images were obtained using a wide-field DeltaVision Elite microscope with a 100× objective using a PCO Edge sCMOS camera ...
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bioRxiv - Genetics 2022Quote: ... Stained gonads were resuspended in 1 drop of Vectashield containing 4’,6-Diamidino-2-Phenylindole Dihydrochloride (DAPI) (Vector Laboratories H-1200), applied to a large pre-made agarose pad on a glass slide ...
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bioRxiv - Neuroscience 2021Quote: ... We then rinsed the sections in PBS and incubated them for 2 h with biotinylated anti‐rabbit IgG secondary antibody (BA‐1000, Vector Laboratories) diluted 1:600 in 4% BSA in 0.4% PBS‐TX ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated with primary antibody for 16-24 hrs at 4°C and then for 2 hrs at 4°C with biotinylated goat anti-rabbit IgG secondary antibody (1:800, Vector Laboratories) in 5% normal goat serum in 0.1% Triton-X-100 in TBS ...
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bioRxiv - Neuroscience 2020Quote: ... After the transfer the blots were blocked for 2 h in 1 x Animal-Free Blocker (SP-5030 Vector Labs, USA). The blots were incubated over night at 4°C under gentle agitation in the primary antibody solutions (1:300 goat anti-UCP1 ...
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bioRxiv - Developmental Biology 2021Quote: ... For IBA1 staining sections were subsequently incubated with a biotinylated F(ab’)2-Goat anti-Rabbit IgG (Vector Labs, CA, USA). All sections were then incubated with horseradish peroxidase (HRP)-conjugated streptavidin (Dako Agilent Pathology Solutions ...
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bioRxiv - Genomics 2022Quote: ... Cells nuclei were counterstained with 1:15,000 DAPI (4′,6-diamidino-2-phenylindole) at room temperature and then mounted onto slides by using the VectaShield (Vector Laboratories) mounting media ...