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Citations for Vector Labs :
601 - 650 of 2433 citations for 4 4 Fluoro 2 hydroxyphenyl methyl 2 6 bis 1 methylethyl 5 propyl 3 pyridinemethanol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... sections were washed and stained with biotinylated anti-mouse IgG (Table 3) in PBST 1 % BSA for 1 h at RT before washing and addition of ABC Elite amplification (Vector Labs) for 30 min at RT according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... together with Fluorescein Avidin DCS (1 μl/ml, Vector Laboratories A-2001-5,) in PBS and 0.3% triton X-100 overnight at 4°C ...
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bioRxiv - Neuroscience 2021Quote: Reagents used were as follows: N-(2-aminoethyl) biotin amide hydrochloride (NEUROBIOTIN™ Tracer, SP-1120, from Vector Laboratories), Alexa 488-conjugated streptavidin (Molecular Probes ...
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bioRxiv - Cell Biology 2023Quote: ... and a hydrophobic barrier was created around the tissue using a hydrophobic barrier pen (Vector Laboratories, H-4000-2). For immunohistochemical (IHC ...
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bioRxiv - Neuroscience 2023Quote: ... The electrodes were filled with 0.5 M K+- acetate and 2% neurobiotin (Vector Laboratories, Burlingame, CA, USA, #SP-1120). Juxtacellular labeling of the recorded neurons was done by filling the neurons with neurobiotin ...
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bioRxiv - Developmental Biology 2024Quote: ... containing 0.3% Tween-20 and blocked with PBS with 0.3% Triton-X100 and 2% normal hose serum (Vector Laboratories # S-2000 ...
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bioRxiv - Genetics 2024Quote: ... Tissue sections were blocked with 2.5% normal donkey serum and 0.1% 4’6-diamidino-2-phenylindole (DAPI, Vector lab, D21490) dissolved in 0.2% PBST for 30 mins after washing with PBS three times ...
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bioRxiv - Neuroscience 2020Quote: ... Tissue was rinsed 3 × 5 min in PBS followed by blocking in 20% normal goat serum (NGS; Vector Labs S-1000, Burlingame, CA, USA) in PBS for 1 hr at RT ...
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bioRxiv - Developmental Biology 2024Quote: ... Tissues were then washed 3 times for 5 minutes each in PT and mounted in Vectashield with DAPI (Vector Laboratories; H-1200-314 10).
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bioRxiv - Physiology 2020Quote: ... Tissue was blocked for 1 hour at room temperature with 3% normal horse serum (Vector Laboratories) in 1X PBS containing 0.1% bovine serum albumin (Sigma-Aldrich) ...
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bioRxiv - Bioengineering 2021Quote: ... Mounting was conducted by incubating for 16 h at 4 °C with VECTASHIELD® PLUS Antifade Mounting Medium (H-1900; Vector Laboratories, Burlingame, CA). Extended information detailing primary antibody ...
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bioRxiv - Microbiology 2022Quote: ... The cells were washed twice with DPBS before the coverslips were mounted on to a glass slide with 4 μl Vectashield with DAPI (Vector Laboratories, cat# H-1200) and sealed with clear nail polish ...
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bioRxiv - Cancer Biology 2023Quote: ... Autofluorescence in tissue sections due to aldehyde fixation was removed by treatment with the TrueVIEW Autofluorescence Quenching Kit for 4 min (Vector Laboratories; SP-8400-15). Sections were counterstained with DAPI (ThermoFisher 62247 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Slides were incubated with the primary antibody overnight at 4 °C and processed using the VECTASTAIN® Elite ABC-HRP Rabbit IgG Kit (Vector Laboratories, #PK-6101) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... Muscle sections were incubated in 1:1000 Wheat germ agglutinin (WGA– fluorescein 5 μg/mL; Cat# FL-1021-5, Vector Laboratories Ltd., Peterborough, UK) for 10 min followed by three washes of PBS-T for 5 min each ...
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bioRxiv - Neuroscience 2020Quote: The next day sections were wash 6 × 10 min with wash buffer (1 × TBS, 0.05% triton X-100) and incubated with biotinylated antibody (Vector Laboratories) diluted 1:200 in secondary blocking buffer (1 × TBS pH 7.6 ...
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bioRxiv - Neuroscience 2024Quote: ... β-amyloid was detected using thiazine red (1 µM, Cat# 2150-33-6, Chemsavers) associated with DAPI (0.5 µM, Cat# 1485, Vector lab) and a mouse monoclonal antibody against VSM α-smooth muscle actin (Cat# ABT1487 ...
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bioRxiv - Genetics 2021Quote: ... To reduce fluorescence fading most slides were mounted in Vectashield antifade mounting medium containing 4.6-diamidino-2-phenylindole (DAPI; Vector Laboratories). Slides stained with Alexa Fluor 660 were stained with DAPI and then mounted in the ProLongTM Gold Antifade Mountant (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... They were then washed and incubated with biotinylated secondary antibody for 2 h at room temperature and developed with Elite ABC kit and 3,3-diaminobenzidine (Vector laboratories). Sections were mounted ...
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bioRxiv - Neuroscience 2023Quote: ... for 2 h at RT followed by incubation in avidin-horseradish peroxidase complex (Vectastain ABC Elite, PK-6000; Vector Laboratories) for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue was then exposed to diaminobenzidine for 2 minutes using the DAB Peroxidase HRP Substrate Kit (Vector Laboratories, SK-4100) then washed with 1X PBS ...
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bioRxiv - Neuroscience 2024Quote: ... Primary antibody was detected using a biotinylated secondary antibody (Table 2) by avidin-biotin peroxidase method (ABC Kit, Vector Laboratories) using diaminobenzidine (DAB ...
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bioRxiv - Biochemistry 2024Quote: ... the samples were washed four times with 1× PBS and mounted in Vectashield Vibrance antifade mounting media with 40,6-diamidino-2-phenylindole (DAPI; catalog no.: H-1800; Vector Laboratories). Images were acquired with a Zeiss LSM 880 confocal microscope with Airyscan ...
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bioRxiv - Cell Biology 2024Quote: ... Nuclei were stained using 49,6-diamidino-2-phenylindole (DAPI) and were subsequently overlaid with Vectashield (Vectashield; Vector Laboratories, Burlingame, CA) mounting medium for imaging with a Zeiss 900 confocal microscope with a 40X objective.
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bioRxiv - Neuroscience 2023Quote: ... Blocking for 1 h with 5% (vol/vol) normal goat serum (NGS, Vector Laboratories) was followed by incubation with the appropriate primary antibody at 4°C for 48 h in 2% (vol/vol ...
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bioRxiv - Neuroscience 2022Quote: ... Blocking for 1 h with 5% (vol/vol) normal goat serum (NGS, Vector Laboratories) was followed by incubation with the appropriate primary antibody at 4°C for 48 h in 2% (vol/vol ...
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bioRxiv - Cancer Biology 2020Quote: ... Primary antibodies were incubated with sections overnight at 4°C and detected using Vectastain Elite ABC HRP Kits (Vector Laboratories: Peroxidase Rabbit IgG PK-6101) and DAB substrate (Vector Laboratories ...
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bioRxiv - Pathology 2022Quote: ... overnight at 4°C then rinsed with TBST prior to detection using an anti-rabbit avidin-biotin amplification kit (Vector Laboratories Cat# PK-6101, RRID:AB_2336820). Vector NovaRed ...
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bioRxiv - Bioengineering 2023Quote: ... Cyanine (Cy)3- and Cy5-conjugated secondary antibodies were used at 1:400 (Vector Laboratories, Burlingame, CA).
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bioRxiv - Cancer Biology 2024Quote: ... Primary antibodies were diluted in 3% BSA with mouse-on-mouse kit (Vector Laboratories, MKB-2213-1), and incubated at 4°C overnight ...
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bioRxiv - Cancer Biology 2024Quote: ... Slides were washed 3 times with PBS-T containing 1% BSA and mounted using VectaShield (Vector Labs). DNA fibers were imaged at 60X with a Nikon 90i microscope.
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bioRxiv - Cancer Biology 2022Quote: ... antibody (1 µg/mL, 3 h; Santa Cruz, #SC-816; secondary: biotinylated goat anti-rabbit IgG, 1:200, 16 minutes; Vector labs, #PK6101), or an anti-c-MYC antibody (1:100 ...
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bioRxiv - Immunology 2020Quote: ... 3-diaminobenzidine (DAB) (Vector Laboratories). Mouse spleen ...
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bioRxiv - Pathology 2020Quote: ... 3’-diaminobenzidine (DAB; Vector Laboratories) was used as a substrate according to the manufacturer’s instructions and hematoxylin was used as a counter-stain ...
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bioRxiv - Molecular Biology 2021Quote: ... Slides were finally mounted with Vectashield Mounting Media with 4,6-diamidino-2-phenylindole as nuclear counterstain (Vector Laboratories, Cat# H-1200). Stainings were digitalized employing the Olympus BX61VS microscope and scanner software VS-ASW-S6 (Olympus ...
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bioRxiv - Genomics 2021Quote: ... Flies were dissected 10 to 40 days pi and organs viewed by fluorescence microscopy and imaged live or fixed in 2% paraformaldehyde and stained with DAPI in VECTASHIELD mounting medium (Vector Laboratories).
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bioRxiv - Neuroscience 2021Quote: ... We then rinsed the sections in PBS and incubated them for 2 h with biotinylated anti‐rabbit IgG secondary antibody (BA‐1000, Vector Laboratories) diluted 1:600 in 4% BSA in 0.4% PBS‐TX ...
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bioRxiv - Developmental Biology 2021Quote: ... For IBA1 staining sections were subsequently incubated with a biotinylated F(ab’)2-Goat anti-Rabbit IgG (Vector Labs, CA, USA). All sections were then incubated with horseradish peroxidase (HRP)-conjugated streptavidin (Dako Agilent Pathology Solutions ...
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bioRxiv - Cell Biology 2020Quote: ... Muscle tissues were then placed into a microcentrifuge tube containing PBT with 2 % final concentration of normal goat serum (NGS; Vector Labs) for 30 mins ...
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bioRxiv - Neuroscience 2020Quote: ... two times for 2 min each before reacting with diaminobenzidine in the presence of nickel (DAB Peroxidase Substrate Kit, Vector Laboratories) according to the manufacturer instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... This was followed by incubation with HRP conjugated 2° antibodies (α-mouse (PI-2000) and α-rabbit (PI-1000, Vector Labs)) in 5% Milk in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... Free-floating sections were washed (three times in 1xPBS for 10 min) and blocked for 2 hours with 10% normal goat serum (NGS, Vector Laboratories), 0.2% BSA (Carl Roth ...
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bioRxiv - Neuroscience 2023Quote: ... The slides were washed in 1x PBS three times for 2 minutes and covered with Vectashield mounting medium without DAPI (Vector Labs). Five imaging fields in the gray matter of the same case were captured using a fluorescent microscope (Keyence BZ-X710) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Expression of MUC protein by Caco-2 cells was studied using biotinylated wheat germ agglutinin (WGA) (Vector Labs; Burlingame, CA, USA). Briefly ...
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bioRxiv - Neuroscience 2022Quote: ... a thin slice of dorsal spinal cord (∼2 mm thick) with attached DR stumps was cut with a microscissors and mounted on slides in Vectashield (Vector Laboratories).
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bioRxiv - Genomics 2022Quote: ... sections were incubated with Alexa dye–conjugated secondary antibodies diluted in blocking serum for 2 hours before being washed again and mounted with Vectashield Antifade Mounting Medium with DAPI (Vector Laboratories). Alexa Fluor® 488 Donkey IgG ...
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bioRxiv - Neuroscience 2023Quote: ... and hybridized with 2 µg/ml digoxigenin (DIG)- and dinitrophenol (DNP)-labeled probes in a hybridization buffer containing the Biotin solution (Vector Laboratories) overnight at 60°C in a humidified chamber ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were dehydrated by incubating for 2 min in increasing concentrations of EtOH (70%, 95%, and 100%) before mounting with Vectashield with DAPI (Vector laboratories). Images of at least 150 nuclei were acquired at 20X using a Zeiss Imager.M2 epifluorescence microscope equipped with a Axiocam 503 mono camera ...
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bioRxiv - Genomics 2024Quote: ... cells were stained with DAPI 2 μg per ml and mounted into slides using Vectashield (Vector Laboratories Cat # H-5700-60). Images were obtained with a Leica TCS-SL confocal microscope ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were then incubated for 2 hours at room temperature with horseradish-peroxidase-labelled secondary antibody (Goat Anti-Rabbit IgG Antibody; Vector Laboratories) diluted 1:10,000 in 5% dried non-fat milk powder in TBS-T ...