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Citations for Vector Labs :
601 - 650 of 848 citations for 2 Methyl 3 4 sulfobutyl benzoxazolium betaine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Primary antibody incubation was completed for 48 hours at 4°C followed by incubation with biotinylated secondary antibodies (Vector Labs) for 24 hours at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... then 4 times 20 min in PBS at RT before leaving brains overnight in Vectashield (Vector Laboratories H-1000-10) at 4C ...
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bioRxiv - Neuroscience 2023Quote: ... Subsequently the sections were incubated with anti-rabbit secondary antibody (1:200 dilution; 4°C; 17h.; Vector Laboratories, Burlingame, USA) followed by tertiary labelling with avidin biotin complex (1:200 ...
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bioRxiv - Biophysics 2024Quote: ... The co-culture was incubated overnight at 4 °C with 10 µg/ml of Tomato Lectin (Vector Laboratories, #DL-1178) in blocking solution ...
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bioRxiv - Neuroscience 2023Quote: ... then incubated overnight in an avidin-biotin-horseradish peroxidase complex at 4°C with 0.1% Triton X-100 (Vector Elite; Vector Laboratories). The tissue was counterstained with cresyl violet and cover slipped with dibutyl phithalate (Electron Microscopy Sciences) ...
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bioRxiv - Cell Biology 2020Quote: ... coverslips were mounted by inversion onto 2 μl of Vectashield Antifade Mounting Medium (Vector Labs H1000). Slides were analysed using an Axioskop2 (Zeiss ...
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bioRxiv - Developmental Biology 2022Quote: ... and cell nuclei were counterstained with 4,6-diamidino-2-phenylindoel (DAPI, Vectashield; Vector Laboratories, Burlingame, USA) 1:10 diluted in fluorescent mounting medium (Serva ...
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bioRxiv - Neuroscience 2021Quote: ... were filled with 1.5 M potassium acetate and 2% Neurobiotin (Vector Labs, Inc; 50-100 MΩ) (59) ...
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bioRxiv - Developmental Biology 2023Quote: ... Lectins used were fluorescein conjugated Lotus tetragonolobus lectin (1:300, LTL, Vector Laboratories, FL-1321-2) and rhodamine conjugated Dolichos biflorus agglutinin (1:100 ...
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bioRxiv - Molecular Biology 2024Quote: ... the spreads were counterstained with 4,6-diamidino-2-phenylindole (DAPI, Vector Laboratories, Inc., Burlingame, CA, USA) for 5 min ...
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bioRxiv - Genetics 2022Quote: ... washed three times in 2% PBTx and mounted in Vectashield with DAPI (Vector Labs, H-1200). Images were obtained with a laser confocal microscope (Zeiss 710 ...
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bioRxiv - Cell Biology 2024Quote: ... The nuclei were then stained with 4,6-diamidino-2-phenylindole (DAPI, Vector Laboratories, cat#H-1200), and sections were coverslipped with antifade mounting media (Vector Laboratories ...
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bioRxiv - Microbiology 2024Quote: ... and detection of SA-α2,3 was performed using biotinylated MAA-1 (B-1315-2, Vector Laboratories) and biotinylated MAA-2 (B-1265-1 ...
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bioRxiv - Immunology 2024Quote: ... slides were mounted using VECTASHIELD Vibrance Antifade Mounting Medium with DAPI (H-1800-2, Vector Laboratories).
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bioRxiv - Neuroscience 2024Quote: ... perineuronal nets were visualized with fluorescently labelled Wisteria floribunda agglutinin (Vector Labs, cat# B-1355-2), which was used in conjunction with primary antibody incubation ...
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bioRxiv - Microbiology 2024Quote: Detection of SA-α2,6 was performed using biotinylated SNA (B-1305-2, Vector Laboratories, California, USA) and detection of SA-α2,3 was performed using biotinylated MAA-1 (B-1315-2 ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated in 3,3’-diaminobenzidine (DAB) solution for 1-2 min (SK-4100, Vector Laboratories) and the DAB staining reaction aborted with 3 washes in ddH2O ...
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bioRxiv - Neuroscience 2024Quote: ... 6-diamidino-2-phenylindole (DAPI) was used (H 1200, Vector Laboratories, Burlingame, CA, USA, Lot # 2E0815). All tissue sections from experimental and control animals were stained at the same time and with the same antibody lot.
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bioRxiv - Cell Biology 2020Quote: ... The coverslips with cells were mounted on 3 cm glass bottom cell culture plates using Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA). Random fields on the coverslips were photographed using an inverted Olympus IX81 microscope (Olympus Europe Holding Gmbh ...
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bioRxiv - Cell Biology 2020Quote: ... Sections were incubated at room temperature with 3% H2O2 for 10 minutes to quench endogenous peroxidase activity and blocked by 5% normal goat serum (Vector Laboratories# S-1000) in TBST (TBS containing 0.1% Tween® 20 ...
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bioRxiv - Neuroscience 2020Quote: ... Endogenous peroxidase activity and non-specific binding were blocked by incubation with 3% hydrogen peroxide in methanol for 15 minutes followed by 5% normal goat serum (Vector Laboratories, CA, USA) with 0.1% TWEEN-20 in PBS for 1 hour ...
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bioRxiv - Neuroscience 2020Quote: ... Brain sections that contained the ventrolateral preoptic nucleus were collected and blocked in PBS containing 0.25% Triton X-100 and 3% normal donkey serum (Vector Laboratories, Burlingame, CA, USA) for 60 min at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... antibody (1 µg/mL, 3 h; Santa Cruz, #SC-816; secondary: biotinylated goat anti-rabbit IgG, 1:200, 16 minutes; Vector labs, #PK6101), or an anti-c-MYC antibody (1:100 ...
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bioRxiv - Neuroscience 2020Quote: Sections were again washed 3×15 min with PBS and incubated in avidin-biotin complex solution (Vectastain Elite ABC kit, Vector Laboratories, Burlingame, CA) (2-hours ...
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bioRxiv - Pathology 2020Quote: ... cryoslides were washed 3 times in PBS and then slides were fluoromounted using Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA, USA).
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bioRxiv - Cell Biology 2020Quote: ... The signal was revealed using Vectastain Elite ABC-HRP kit and AEC Peroxidase (HRP) substrate 3-amino-9ethylcarbazole (VECTOR AEC Peroxidase Subtract Kit SK-4200, Vector Laboratories, Burlingame, CA). Samples were mounted using ProLong Gold antifade reagent (Life Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... The slides were then rinsed in diH2O and counterstained in a 1:3 diluted solution of Nuclear fast red (Vector labs H-3403). Counterstaining was done for no longer than 3 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... In the end slides were washed 3 times with 0.1%Tween in PBS and mounted with VECTASHIELD Antifade Mounting Medium with DAPI (Vector Laboratories, H-1200-10). The images were acquired using Zeiss LSM800 confocal microscope and analyses using Fiji software 86 and visualized and deposited using OMERO 87 (project ID:12920).
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bioRxiv - Neuroscience 2022Quote: ... Sections were then washed 3 times with PBS and mounted on slides with VECTASHIELD® Vibrance™ Antifade Mounting Medium with DAPI (Vector laboratories).
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bioRxiv - Neuroscience 2022Quote: The sections were rinsed in PB and to block non-specific antibody binding they were preincubated for 1 h at room temperature in a stock solution containing 3% normal serum of the species in which the secondary antibodies were raised (Vector Laboratories, Burlingame, CA) in PB with Triton X-100 (0.25%) ...
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bioRxiv - Cell Biology 2024Quote: ... Peroxidase was quenched by incubating them in 3% hydrogen peroxide (H2O2) for 10 min and they were then blocked with 1% normal horse serum (Vector Laboratories, SP-2001) at room temperature for 15 min ...
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bioRxiv - Cell Biology 2023Quote: ... slides were washed in 3 x 5 min in TBST and mounted with Vectashield® mounting medium (Cat. No. H-1000; Vector Laboratories, USA).
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bioRxiv - Neuroscience 2023Quote: ... 0.3% Triton X-100 (24 hours, 37°C) followed by peroxidase-conjugated secondary antibody (1:200 biotinylated anti-rabbit Vector Labs #BA-1000) and incubated in Elite ABC solution ...
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bioRxiv - Bioengineering 2022Quote: ... Retinas were then washed 3 times in PBS at RT and incubated with polyclonal rabbit LM opsin and S opsin antibody (1/300; Vector Laboratories, Peterborough, UK) for 48 hours at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... the slides were incubated with secondary antibodies for 1 hour and washed again 3 times with PBS and mounted with Vectaschield containing DAPI (Vector Laboratories, H-1500).
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bioRxiv - Neuroscience 2024Quote: ... slides were washed with PBS and incubated for at least an hour with blocking buffer containing 3% normal goat serum (NGS; Vector Laboratories, Burlingame, CA) and 0.4% Triton X-100 (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were washed 3 times with PBST for 5 min each and mounted with Vectashield vibrance with DAPI (Cat. # H-1800-10, Vector Labs, Newark, CA). Cells were imaged at 63x on the confocal 60 μm disk setting with the BioTek Cytation C10 using the Gen5 software (Agilent) ...
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bioRxiv - Cell Biology 2024Quote: ... and after 3 more washes with PBS the coverslips were mounted with Vectashield solution (mounting medium with DAPI, Vector Laboratories Inc., Burlingame, CA). Evaluation was done using a Nikon 80i or Bio-rad confocal microscope ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated in biotinylated goat-anti-mouse antibody for 24 hours at 4 °C in blocking solution (1:200, BA-1000, Vector Laboratories). Color reaction was performed using the Vectastain Elite ABC Kit (PK-6100 ...
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bioRxiv - Genetics 2020Quote: ... Sections were incubated with primary antibody (supplementary table S4) overnight at 4°C and with universal biotinylated horse anti-mouse/rabbit immunoglobulin G secondary antibody (Vector Laboratories) for 1 hour ...
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bioRxiv - Physiology 2021Quote: ... Ears were then gently shaken (120 rpm) at 4°C overnight in 20 μg/ml Dylight 488 strepavidin (SA-5488, Vector Laboratories) and 0.05 mg/ml DAPI (D9542 ...
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bioRxiv - Neuroscience 2021Quote: ... Primary and secondary antibodies were incubated for >36h at 4°C in wash buffer (PBS + 1% BSA) and mounted in Vectashield (Vector Labs). Antibodies used were mouse anti-abd-A (gift of Ian Duncan) ...
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bioRxiv - Physiology 2021Quote: ... Frozen bone sections were incubated for 4 min at room temperature in Alkaline Phosphatase Substrate Solution ImmPACT Vector Red (Vector Laboratories). After washing with buffer ...
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bioRxiv - Microbiology 2021Quote: ... ovaries were fixed in 4% PFA and then mounted in Vectashield® mounting media with DAPI counterstain (Vector Laboratories, Burlingame, CA). Ovaries were then imaged using Differential Interference Contrast on a Zeiss Inverted Observer Z1 at 100X magnification.
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bioRxiv - Neuroscience 2020Quote: ... Tissue was rinsed five times for ten minutes in PBS and then incubated overnight at 4°C in a rabbit anti-goat secondary (1:500; Vector Laboratories) diluted in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... PV staining was performed as above and then sections were incubated at 4°C overnight with byotinilated VVA (1:5000, #B-1235, RRID: AB_2336855, Vector Laboratories, USA). After rinsing ...
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bioRxiv - Cancer Biology 2022Quote: ... or CD4 (ab183685, 1:400 dilution overnight at 4 degrees).Tissues were then counterstained with biotinylated anti-rabbit secondary (Vector Labs, BA-1000 ...
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bioRxiv - Neuroscience 2020Quote: ... Primary and secondary antibodies were incubated for >36h at 4°C in wash buffer (PBS 1% BSA) and mounted in Vectashield (Vector Labs). Antibodies used were rabbit and guinea pig anti-Hth (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were blocked with 10% normal goat or horse serum followed by overnight incubation with primary antibody at 4°C and 30 min incubation with ImmPRESS HRP-conjugated secondary antibodies (Vector Laboratories) at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... sections were incubated with primary antibodies at 4°C overnight followed with biotinylated HRP-conjugated secondary antibody (Vector Laboratories # BA-1000) at room temperature for 1 hour ...