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Citations for Vector Labs :
551 - 600 of 1065 citations for SARS CoV 2 Spike Glycoprotein S2 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... IHC signal was amplified by using biotin conjugated secondary antibodies combined with VECTASTAIN® Elite ABC-HRP Kit (Vector laboratories) and images acquired with a Zeiss Z1 scanner ...
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bioRxiv - Microbiology 2019Quote: ... cells were permeabilized by 0.1% saponin (in FACS-B with 1% normal mouse serum) with streptavidin added (Streptavidin/Biotin Blocking Kit, Vector Laboratories) to block endogenous biotin (permeabilizing and blocking solution) ...
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bioRxiv - Systems Biology 2019Quote: ... The 5-end cap structure was biotinylated by sequential oxidation with NaIO4 and biotinylation with biotin hydrazide (Vector Laboratories, USA). After RNase I treatment (Promega ...
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bioRxiv - Neuroscience 2019Quote: ... diluted 1:200 in KPBS containing 3 % NGS and then for 1 h at RT in an avidin-biotin-peroxidase complex solution prepared in KPBS according to the manufacturer’s recommendations (Vectastain ABC kit, Vector Laboratories). Sections from VGLUT2-EYFP and VGLUT2-ChR2 mice were processed In parallel and for the same period of time (15 min ...
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bioRxiv - Immunology 2019Quote: ... processed with an avidin/biotin staining kit with 3,3-diaminobenzidene (DAB) as the chromogen (Vector ABC Elite Kit and DAB Kit, Vector Laboratories), then counterstained with hematoxylin [29] ...
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bioRxiv - Cell Biology 2019Quote: ... The 5-end cap structure was biotinylated by sequential oxidation with NaIO4 and biotinylation with biotin hydrazide (Vector Laboratories, USA). After RNase I treatment (Promega ...
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bioRxiv - Cell Biology 2021Quote: ... For Lotus Tetragonolobus Lectin (LTL biotinylated) staining sections were additionally blocked with a streptavidin/biotin blocking kit (both Vector Laboratories) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Tissue sections were fixed with ice cold acetone for 5 min and blocked with Avidin/Biotin Blocking Kit (Vector laboratories) and Protein block (Protein block (Dako ...
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bioRxiv - Developmental Biology 2022Quote: ... and Ki67 immunostaining signals were further amplified by tyramide-FITC or tyramide-Cy3 (1:2,000, Perkin-Elmer, Wellesley, MA) after avidin-biotin complex incubation (Elite ABC kit, Vector Laboratories). Immunostained sections were counterstained with DAPI (1 μg /ml ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were washed with PBS and incubated for 30 min with the avidin-biotin complex (Vector Laboratories, Burlingame CA) and then incubated for 5 min in 3,3’-diaminobenzidine (DAB)/H2O2 (Sigma Fast ...
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bioRxiv - Neuroscience 2022Quote: ... The sections were then incubated for 1 h at room temperature with biotin-conjugated goat anti-mouse secondary antibody (Cat#BA-9200-1.5, Vector Laboratories) at 1:200 dilution ...
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bioRxiv - Genomics 2023Quote: The aldehyde groups resulting in a 40 μl mix were then biotinylated using a mixture containing 4 μl of NaOAc (1M, pH 6.0) and 4 μl of Biotin (Long Arm) Hydrazide (100 mM, catalog num. SP-1100, Vector Laboratories). The 48 μl resulting mixture was next incubated for 30 minutes at 40°C ...
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bioRxiv - Pathology 2023Quote: ... and 3,3’-Diaminobenzidine tetrahydrochloride (DAB, Carl Roth) as previously described 34 or using avidin–biotin complex (ABC) peroxidase kit (Vector Labs) and DAB (Carl Roth ...
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bioRxiv - Cancer Biology 2023Quote: ... A biotinylated secondary antibody was used to amplify the signal using an avidin–biotin substrate (Vector Laboratories, Inc., Newark, CA). Immunohistofluorescence staining for γH2AX was done as described in Wang et al 23.
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bioRxiv - Neuroscience 2023Quote: ... then incubated overnight in an avidin-biotin-horseradish peroxidase complex at 4°C with 0.1% Triton X-100 (Vector Elite; Vector Laboratories). The tissue was counterstained with cresyl violet and cover slipped with dibutyl phithalate (Electron Microscopy Sciences) ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were blocked with 5% normal goat serum followed by an Avidin/Biotin blocking kit (Vector Labs, Burlingame, CA, USA) and then incubated overnight at room temperature with the primary antibody ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated in the biotinylated goat anti-rabbit secondary antibody and avidin–biotin–peroxidase complex (ABC Elite; Vector Laboratories). They were washed in PBS and reacted with diaminobenzidine (DAB free base ...
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bioRxiv - Neuroscience 2024Quote: ... After an hour of cool-down slides were washed with PBS and incubated with blocking buffer (1% BSA, 5% FBS, 0.01 % Triton-X 100 in 1x PBS) followed by Avidin/Biotin blocking (Vector laboratories). Slides were incubated overnight at 4°C with primary antibodies (see Supplementary Table 4 ...
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bioRxiv - Physiology 2019Quote: ... Tissue slides were then acetone-fixed followed by incubation with Fc-receptor blocking in 2.5% goat serum (Vector Laboratories) and incubation with primary antibodies cocktail containing anti-CD11b:AlexaFluor488 and CD11c:PE (Biolegend) ...
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bioRxiv - Molecular Biology 2019Quote: ... PB and E samples were subjected to SDS-PAGE and Western blotting followed by biotin detection using the Vectastain ABC AmP Reagent and Duolux chemiluminescent substrate (Vector Labs) according to the manufacturer’s directions ...
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bioRxiv - Biochemistry 2019Quote: ... was carried out using a biotinylated secondary antibody together with the avidin-biotin system and the VECTOR peroxidase kit (PK-6100, Vector Laboratories) using diaminobenzidine tablets as a substrate.
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bioRxiv - Biophysics 2021Quote: ... Biotinlabelled probes were detected using one layer of FITC-conjugated streptavidin followed by a layer of biotin-conjugated anti-avidin and a second layer of FITC-conjugated streptavidin (Vector Laboratories). Slides were counter-stained with 0.5 µg ml−1 DAPI.
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bioRxiv - Neuroscience 2021Quote: Sections used for morphological analyses in Figure 1C were further incubated in a solution of conjugated avidin-biotin horseradish peroxidase (ABC; 1:300; Vector Labs) in TBS (pH = 7.4 ...
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bioRxiv - Cancer Biology 2019Quote: ... for 1 h at room temperature or overnight at 4°C and avidin/biotin or polymer horse radish peroxidase kits for primary mouse or rabbit antibodies (Vector Laboratories) were used for detection ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were processed the following day using a Vectastain kit (biotinylated goat-anti-rabbit igG secondary antibody and avidin-biotin-peroxidase complex) and ImpacDAB (Vector Laboratories). Control sections lacking the primary antibody were unlabeled ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated with anti-rat biotinylated secondary antibody (2h, room temperature) and processed with avidin-biotin-peroxydase complex (ABC Elite Kit, Vector Laboratories) for 30 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... sections were washed in TBS and incubated for 1 hr with horseradish peroxidase-labeled avidin-biotin complex (ABC complex, 1:400, Vector Labs) at RT ...
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bioRxiv - Neuroscience 2022Quote: ... tissues were incubated for 1-hour in an avidin biotin complex solution (per kit instructions, ABC Peroxidase, PK-6100, Vector Laboratories) at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... The wells were then washed twice with PBS and reacted with avidin/biotin-HRP complex (VECTASTAIN Elite ABC kit; Vector Laboratories) at 50 μL/well at 4 °C for 1 h ...
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bioRxiv - Neuroscience 2021Quote: ... We rinsed the sections again in PBS and incubated them in avidin–biotin– peroxidase complex (ABC Elite kit, PK‐6100, Vector Laboratories) in 0.5% PBS‐TX for 1 h ...
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bioRxiv - Immunology 2021Quote: ... Color was developed by incubating the slides with freshly prepared avidin-biotin-peroxidase complex (ABC) solution (Vectastain Elite ABC Kit; Vector Laboratories), followed by exposure to 3-amino-9-ethylcarbazole substrate (AEC ...
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bioRxiv - Neuroscience 2020Quote: ... the tissue was incubated in biotinylated secondary antibody followed by incubation with the avidin-biotin complex solution (Vectastain ABC kit, Vector Laboratories). Immunoreactivity was visualized using standard DAB procedures ...
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bioRxiv - Developmental Biology 2021Quote: ... Visualization of eRNAi clones was achieved by detecting the co-injected biotin-dextran with the Vectastain ABC-AP kit (Vector Laboratories) after ISH ...
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bioRxiv - Neuroscience 2021Quote: ... Cleaved caspase 3 was detected on frozen sections of conditional Bcl6 mutants using an avidin/biotin-based peroxidase system and DAB substrate (Vector Laboratories). All fluorescent images were examined on a TCS SP5II confocal microscope (Leica ...
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bioRxiv - Neuroscience 2021Quote: ... visualization of antigen-bound primary antibodies was carried out using a biotinylated secondary antibody together with the avidin-biotin system and the VECTOR peroxidase kit (PK-6100, Vector Laboratories) using both diaminobenzidine tablets (D4293 ...
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bioRxiv - Bioengineering 2021Quote: ... Sections were then washed thrice in phosphate buffered saline prior to 30 min incubation with biotin-conjugated Goat Anti-Mouse IgG (H+L) (1:500, BP-9200, Vector Laboratories). Endogenous peroxidase was blocked and detection was performed using VECTASTAIN® ABC-HRP Kit with signal development carried out using 3,3-diaminobenzidine ...
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bioRxiv - Pathology 2020Quote: ... The sections were then rinsed in PBS and allowed to react with avidin-biotin peroxidase complexes (VECTASTAIN Elite ABC kit, Vector Laboratories) for 60 min at 37°C ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by subsequent processing by the indirect streptavidin-biotin-peroxidase method using the Vectastain Elite kit (Vector Laboratories, Burlingame, CA, USA) and nickel-sulphate-based chromogen enhancement detection as previously described (Bartkova et al. ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... was used at 5 μg/mL final concentration for 1 hour at 24°C following by goat anti-rabbit IgG biotin conjugate used as secondary antibody at 1-200 final dilution (BA-1000, Vector Laboratories). DAB Map including 3,3-Diaminobenzidine chromogen was applied to the slides for histological staining.
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bioRxiv - Neuroscience 2020Quote: ... Sections were then washed five times for ten minutes in PBS and then incubated in an avidin-biotin horseradish peroxidase complex (1:1000; ABC Vector Elite kit; Vector Laboratories) for 4 h at room temperature ...
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bioRxiv - Immunology 2021Quote: ... The red-brown antigen labelling was developed by application of avidin-biotin-peroxidase complex (ABC) solution (Vectastain ABC Kit, #PK 6100, Vector Laboratories), followed by exposure to 3-amino-9-ethylcarbazole substrate (AEC ...
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bioRxiv - Neuroscience 2019Quote: ... the tissue was incubated for 40 min in biotinylated secondary anti-rabbit antibody made in goat (1:200; Vector BA-1000) followed by incubation with the avidin–biotin complex solution (Vectastain ABC kit, Vector Laboratories). Immunoreactivity was visualized using standard DAB procedures ...
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bioRxiv - Cancer Biology 2020Quote: ... after washing sections were incubated with biotinylated secondary antibody at room temperature for 1 hour followed by incubation with ABC (Avidin Biotin complex) (Vector Labs) for 30 minutes ...
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bioRxiv - Developmental Biology 2021Quote: ... the slides were washed with PBS (pH 7.4) and submitted to the avidin-biotin-peroxidase solution (ABC Kit -PK -6100 Vector Laboratories®) for 45 minutes ...
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bioRxiv - Immunology 2020Quote: ... Slides were incubated with primary antibody for overnight and secondary antibody for 20 minutes and then the staining was amplified using avidin-biotin-peroxidase conjugate (ABC elite; Vector Laboratories) and visualized using 3 ...
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bioRxiv - Neuroscience 2022Quote: ... Immunohistochemical reactions on free-floating brain and spinal cord sections using the avidin-biotin peroxidase method (Vectastain, Vector Laboratories, Burlingame, CA) were used to identify rabies-infected neurons ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then wash 3 times for ten minutes each in TBST and then incubated with prepared elite avidin biotin complex (ABC) reagent (Vector Laboratories) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated with a solution of avidin-biotin-peroxidase complex (1:100; VECTASTAIN Elite ABC Standard Kit, Vector Laboratories) overnight ...
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bioRxiv - Neuroscience 2023Quote: ... followed by additional rinses and an overnight incubation in avidin biotin complex (Vectastain Elite ABC kit HRP, standard, cat#PK-6100, Vector Laboratories). The next day sections were rinsed in PBS and incubated in a solution containing 3’3’-diaminobezidine tetrahydrochloride hydrate (DAB ...
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bioRxiv - Molecular Biology 2023Quote: ... free-floating sections were rinsed and bound antibodies were visualized by the Avidin-Biotin complex (ABC) amplification system (Vectastain ABC kit, Vector Laboratories) using 3,3′-diaminobenzidine tetrahydrochloride (DAB Substrate Kit ...