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Citations for Vector Labs :
551 - 600 of 688 citations for 4 Methoxy 6 methyl 6 phenyl 5H pyran 2 one since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... Immunostained preparations were mounted in VECTASHIELD® Antifade Mounting Medium containing DAPI (4,6 diamidino-2-phenylindole) (VECTOR Laboratories). To quantify the foci at least 1000 cells were analyzed for each PIR ...
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bioRxiv - Biophysics 2023Quote: ... Cells were mounted on 20 x 75 x 1.0 mm slide using vectashield (Vector Laboratories, H-1700-2) and sealed with nail polish 1 hour later.
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bioRxiv - Cancer Biology 2024Quote: ... Microvessels were stained with 2 mg/mL Fluorescein Ulex Europaeus Agglutinin 1 (UEA1, Vector Laboratories, Burlingame, CA, USA) in EGM-2 daily until microscope observation ...
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bioRxiv - Neuroscience 2022Quote: ... and in TBS-Tx (2 × 5min) and incubated with a Vector ABC kit (Vector laboratories, Inc., Burlingame, USA) for 90 min at RT ...
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bioRxiv - Neuroscience 2023Quote: ... slides were incubated with Vector hematoxylin QS (2 min) to counterstain nuclei (Vector Hematoxylin QS Kit, Vector Laboratories). After dehydration in ethanol series and clearing in xylene they were again mounted using VectaMount (all steps as described for DAB-staining).
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bioRxiv - Immunology 2022Quote: ... The second primary antibody was added overnight at 4°C and visualised the following day using Vector Red Substrate Kit (Vector Labs, SK-5100). Slides were counterstained with haematoxylin ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were then washed with 0.1% PBST for 3 × 10 minutes and carefully cut into 4 quadrants and mounted on slides with DAPI Vectashield (Vector Laboratories, H-1200). For cryosections ...
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bioRxiv - Neuroscience 2020Quote: ... tissue sections were rinsed and incubated at room temperature in a secondary biotinylated anti-mouse antibody for 4 hours (Vector Laboratories, Burlingame, CA). This series was then incubated with ABC solution and reacted with Vector SG (Vector Laboratories ...
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bioRxiv - Bioengineering 2020Quote: ... gels were fixed with 4% paraformaldehyde and stained with 10 mg/mL rhodamine-labeled Griffonia simplicifolia lectin I (GS-1lectin; Vector Laboratories; Burlingame, CA). Five randomly selected fields were imaged per gel to an area of 3×3 mm and a depth of ∼500 µm using a Zeiss LSM 700 confocal microscope ...
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bioRxiv - Physiology 2023Quote: ... the cells were fixed with 4% PFA at 4 ℃ for 20 minutes in the dark and mounted using VECTASHIELD® HardSet™ Antifade Mounting Medium (#H-1400-10, Vector Laboratories). Cell nuclei were stained with DAPI ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated overnight at 4 °C with STL-FITC (dilution 1:750, solanum tuberosum lectin-fluorescein isothiocyanate, endothelial marker, Vector Laboratories FL-1161) and goat anti-IBA1 (dilution 1:1500 ...
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bioRxiv - Developmental Biology 2023Quote: ... in 1% NDS in PBS/MT at 4°C for 24 hr and subjected to peroxidase-conjugated streptavidin treatment in blocking solution (1:500; PK6200, Vector Laboratories, Burlingame, CA) at 4°C for 24 hr after rinsing in PBS/MT on ice (1 hr x 5) ...
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bioRxiv - Cell Biology 2023Quote: ... melanogaster larval imaginal discs were dissected in PBS and fixed in 4% paraformaldehyde and either mounted directly in VectaShield Mounting Medium (Vector Laboratories, H-1000) or first permeabilised with PBST (PBS with 0.3% Triton X-100 ...
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bioRxiv - Cancer Biology 2023Quote: ... Slides were incubated with primary antibodies overnight at 4°C and with secondary antibodies for 1 hr at room temperature (RT) before ABC-HRP (Vector Laboratories #PK-7100) treatment and DAB (Vector Laboratories #sk-4105 ...
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bioRxiv - Cell Biology 2024Quote: ... Cultures were subsequently fixed in 4% PFA and stained for ALP using the Vector Blue Alkaline Phosphatase Substrate Kit (Vector Laboratories SK-5300) and Von Kossa using a 4% silver nitrate solution and exposing cultures to 2400 kJ of ultraviolet light using a UV Stratalinker.
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bioRxiv - Physiology 2024Quote: ... mouse pancreatic sections were incubated overnight at 4°C in guinea pig anti-insulin antibody (1:800) and visualized using the diaminobenzidine peroxidase substrate (Vector laboratories, Burlingame, CA) and hematoxylin staining ...
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bioRxiv - Neuroscience 2020Quote: ... filled with a chloride solution 0.5 M containing neurobiotin tracer (1-2%, w/v; CAT#SP-1120, Vector laboratories) to perform juxtacellular labelling ...
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bioRxiv - Neuroscience 2021Quote: Reagents used were as follows: N-(2-aminoethyl) biotin amide hydrochloride (NEUROBIOTIN™ Tracer, SP-1120, from Vector Laboratories), Alexa 488-conjugated streptavidin (Molecular Probes ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... Sections were then incubated at room temperature for 2 h in biotinylated goat anti-mouse (1:200, Vector Laboratories). Brain sections were further processed using the Avidin-Biotin Complex (ABC Elite kit ...
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bioRxiv - Neuroscience 2022Quote: ... followed by 2 h incubation in a solution containing avidin-biotin-peroxidase complex (ABC, 1:300 dilution, Vector Laboratories). Finally ...
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bioRxiv - Neuroscience 2022Quote: ... followed by 2 h incubation in a solution containing avidin–biotin–peroxidase complex (ABC, 1:300 dilution, Vector Laboratories). Finally ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated for 2 h at room temperature with species-specific biotinylated secondary antibody (1:200 dilution) (Vector Laboratories) in 0.1M PBS ...
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bioRxiv - Developmental Biology 2024Quote: ... containing 0.3% Tween-20 and blocked with PBS with 0.3% Triton-X100 and 2% normal hose serum (Vector Laboratories # S-2000 ...
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bioRxiv - Cell Biology 2023Quote: ... and a hydrophobic barrier was created around the tissue using a hydrophobic barrier pen (Vector Laboratories, H-4000-2). For immunohistochemical (IHC ...
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bioRxiv - Neuroscience 2023Quote: ... The electrodes were filled with 0.5 M K+- acetate and 2% neurobiotin (Vector Laboratories, Burlingame, CA, USA, #SP-1120). Juxtacellular labeling of the recorded neurons was done by filling the neurons with neurobiotin ...
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bioRxiv - Genetics 2024Quote: ... Tissue sections were blocked with 2.5% normal donkey serum and 0.1% 4’6-diamidino-2-phenylindole (DAPI, Vector lab, D21490) dissolved in 0.2% PBST for 30 mins after washing with PBS three times ...
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bioRxiv - Genetics 2024Quote: ... The chromosomes were counterstained with 5 μg/mL of 4,6-diamidino-2-phenylindole (DAPI) in Vectashield (Vector Laboratories, USA) then observed under a BX43 phase-contrast microscope or a BX-53 fluorescence microscope (both from Olympus ...
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bioRxiv - Bioengineering 2021Quote: ... Mounting was conducted by incubating for 16 h at 4 °C with VECTASHIELD® PLUS Antifade Mounting Medium (H-1900; Vector Laboratories, Burlingame, CA). Extended information detailing primary antibody ...
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bioRxiv - Neuroscience 2022Quote: ... sections were washed 5x in 0.1M PBS for 10 min per wash and incubated overnight at 4°C in secondary antibody (biotinylated goat anti-rabbit IgG; 1:500; Vector Laboratories, Inc, Burlingame, CA). The last day ...
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bioRxiv - Neuroscience 2022Quote: ... slices were rinsed again 4 times for 5 minutes in PBS and revealed using a DAB substrate kit (Vector Laboratories Cat# SK-4100, RRID:AB_2336382). Stained slices (at least 5 per region ...
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bioRxiv - Microbiology 2022Quote: ... The cells were washed twice with DPBS before the coverslips were mounted on to a glass slide with 4 μl Vectashield with DAPI (Vector Laboratories, cat# H-1200) and sealed with clear nail polish ...
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bioRxiv - Cancer Biology 2023Quote: ... Autofluorescence in tissue sections due to aldehyde fixation was removed by treatment with the TrueVIEW Autofluorescence Quenching Kit for 4 min (Vector Laboratories; SP-8400-15). Sections were counterstained with DAPI (ThermoFisher 62247 ...
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bioRxiv - Microbiology 2024Quote: ... and mounted on a glass microscope slide using a 4:1 mixture of Citiflour™ AFI mountant solution to Vectashield® vibrance antifade mounting medium (Vector Laboratories, USA). The fluorescence images were taken using an Echo Revolve R4K (Upright & Inverted Capability ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Slides were incubated with the primary antibody overnight at 4 °C and processed using the VECTASTAIN® Elite ABC-HRP Rabbit IgG Kit (Vector Laboratories, #PK-6101) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Cryosections were blocked in PBS containing 10% donkey serum and 0.1% Triton X-100 for 2 h and incubated overnight at 4°C with rabbit anti-Iba-1 and goat anti-Arg1 antibodies or FITC-conjugated Lycopersicon esculentum (Tomato) Lectin (Vector Labs, FL-1171-1). Next ...
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bioRxiv - Genetics 2021Quote: ... To reduce fluorescence fading most slides were mounted in Vectashield antifade mounting medium containing 4.6-diamidino-2-phenylindole (DAPI; Vector Laboratories). Slides stained with Alexa Fluor 660 were stained with DAPI and then mounted in the ProLongTM Gold Antifade Mountant (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... for 2 hrs at room temperature before 10 min staining with DAPI (1:10,000) and mounting in Vectashield (Vector Laboratories). Mean fluorescence was measured with Fiji (https://imagej.net/fiji ...
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bioRxiv - Microbiology 2020Quote: ... Coverslips were mounted using Vectashield mounting medium supplemented with 1 ug/ml 4,6-diamidino-2-phenylindole (DAPI; Vector Laboratories, Inc.). Slides were examined on a Zeiss Axiovert 200 microscope with an AxioCam camera and ZEN Pro software (Carl Zeiss ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were rinsed with TBS-T (0.1% Tween-20 in TBS) 2 times for 10 min and block with 1.5% horse serum (S-2000, Vector Laboratories) in TBS-T for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Floating sections were blocked for 1 hour at room temperature in 2% normal donkey serum (NDS, Vector Laboratories, Burlingame, CA) in 0.3% Triton X-100 PBS ...
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bioRxiv - Microbiology 2020Quote: ... sections were incubated for 2 hours with SNL-EBL conjugated to Cy5 (1:50 dilution, Vector Labs, cat # CL-1303) followed by 1 hour with Spn typing sera (1:2000 dilution ...
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bioRxiv - Physiology 2020Quote: ... incubated in 4’6’-diamidino-2-phenylindole (DAPI) (1:1000) in TBS for 5 minutes then mounted in Vectashield (Vector Laboratories). Fluorescent images were captured using a Nikon A1R-s confocal laser scanning microscope at the BCM Integrated Microscopy Core and processed with the Nikon Perfect Focus System (Nikon Corporation ...
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bioRxiv - Neuroscience 2021Quote: ... They were then washed and incubated with biotinylated secondary antibody for 2 h at room temperature and developed with Elite ABC kit and 3,3-diaminobenzidine (Vector laboratories). Sections were mounted ...
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bioRxiv - Genetics 2021Quote: ... the sections were washed 3 times in PBS for 5 minutes each and mounted with Vectashield mounting medium containing 4,6-diamidino-2-phenylindole (DAPI, Vector Laboratories) and sealed with nail polish ...
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bioRxiv - Neuroscience 2024Quote: ... washed and incubated in horse anti-mouse biotinylated secondary (1:200 in 2% BSA and 0.1% Triton {TBS]; Vector Laboratories) for 180 min prior to washing and development in Streptavidin-tertiary reagent (1:500 in 2% BSA and 0.1% Triton [TBS] ...
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bioRxiv - Neuroscience 2024Quote: ... incubated in secondary antibody in 5% NGS for at least 2 overnights and mounted using Vectashield (Vector Laboratories H-1000). After each step ...
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bioRxiv - Neuroscience 2022Quote: ... and counterstained with 1 μg/mL DAPI (Thermo) for 2 minutes to label nuclei and mounted with VectaShield (Vector Laboratories). Images were taken using a confocal microscope (Zeiss LSM 800) ...
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bioRxiv - Molecular Biology 2023Quote: ... Double blocking was achieved with (1) H2O2 for 10 mins and (2) 2.5% normal horse serum for 1 hour (S-2012, Vector Labs). vWAT sections were incubated overnight at 4°C with primary antibody (CD68 ...
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bioRxiv - Neuroscience 2023Quote: ... for 2 h at RT followed by incubation in avidin-horseradish peroxidase complex (Vectastain ABC Elite, PK-6000; Vector Laboratories) for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue was then exposed to diaminobenzidine for 2 minutes using the DAB Peroxidase HRP Substrate Kit (Vector Laboratories, SK-4100) then washed with 1X PBS ...