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Citations for Vector Labs :
551 - 600 of 616 citations for 3 O tert Butyldimethylsilyl 24 ethyl 24 phenylsulfonyl cholest 5 ene 3 ol d7 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Slides were blocked/permeabilized for 1 hour at room temperature with a solution of 5% Normal Horse Serum (Vector Laboratories, Burlingame, California), 0.2% Triton X-100 (Amresco ...
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bioRxiv - Neuroscience 2022Quote: ... or Phaseolus vulgaris leucoagglutinin (PHA-L; 2.5% solution in 10 mM phosphate buffer, pH 7.4; Vector Laboratories, Cat. No. L-1110-5). To reduce the number of animals ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were washed 4 × 10min in TBS 0.1% Tween and incubated with one of the following peroxidase-conjugated secondary antibodies diluted in TBS 0.1% Tween supplemented with 5%BSA for 2h at room temperature: horse anti-mouse IgG(H+L) (Vector laboratories, PI-2000), goat anti-rabbit IgG(H+L ...
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bioRxiv - Neuroscience 2023Quote: ... slides were deparaffinized and boiled for 20 min in citrate buffer (0.1M sodium citrate and 0.1M citric acid in distilled H2O) for antigen retrieval and incubated with 5% normal rabbit serum (Vector laboratory #S-5000) in PBS containing 0.2% Triton for 1 h at RT to block unspecific binding ...
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bioRxiv - Cancer Biology 2024Quote: ... Sections were washed with 1x PBS for 5 min and antigen retrieval was performed with Antigen Unmasking Solution Citrate-based pH 6.0 (Vector Laboratories H-3300) in a Decloaking Chamber™ (Biocare Medical ...
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bioRxiv - Cell Biology 2024Quote: ... After blocking with 5% goat serum/1% bovine serum albumin/PBS and Mouse on Mouse blocking reagent (Vector Laboratories, Newark, CA, USA), the samples were incubated with primary antibodies overnight at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: Immunohistochemistry on 5 µm sections from frozen or paralin-embedded tissues was performed using the biotin-peroxidase method (Vector laboratories, Burlingame, CA). IHC staining and scoring were evaluated by a trained pathologist.
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bioRxiv - Physiology 2024Quote: ... Cardiac sections were then washed in PBST and labeled with FITC conjugated Wheat-Germ Agglutinin (1:500; Vector Labs #FL-1021-5) and Goat anti-Mouse IgM Alexa Fluor 594 (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... tissue was rinsed again three times (5 minutes each) in 0.01M PBS before immersing in an avidin-biotin complex (ABC; Vector labs, PK-4000) in the same concentration PBS for 30 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... followed by washing in ultrapure water for 5 min and mounted with Vectashield Vibrance® Antifade Mounting Medium (# ZKO8O3, Vector Labs, US) with or without DAPI (0.001 mg/ml) ...
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bioRxiv - Cancer Biology 2024Quote: ... The incubation with the primary antibody is done for 5 hours followed by biotinylated mouse secondary (Vector Labs, MOM Kit BMK-2202) in 5.75μg/mL ...
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bioRxiv - Neuroscience 2021Quote: ... incubated in 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked in 5% normal goat serum (NGS) (Vector Labs, Burlingame, CA) with 0.1% Triton X-100 ...
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bioRxiv - Synthetic Biology 2021Quote: ... blocked in 5% bovine serum albumin for 30 min and probed for 1 h with fluorescein-labeled SBA (Vector Laboratories, catalog # FL-1011) and Alexa Fluor 647® (AF647)-conjugated anti-His antibody (R&D Systems ...
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bioRxiv - Cell Biology 2021Quote: ... the retinal sections were washed with PBS three times for 5 min each and then mounted with Vectashield Mounting Medium (Vector Laboratories, Burlingame, CA). The images were acquired with a BZ-X810 Keyence microscope (Keyence ...
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bioRxiv - Physiology 2021Quote: ... Sections were incubated at room temperature with 3% (v/v) H2O2 for 10 min to quench endogenous peroxidase activity and blocked by 5% (v/v) normal goat serum (Vector Laboratories, #S-1000) in PBS for 1 hour ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were rinsed again in PBS and then incubated for 1 hr in avidin-biotin complex (4.5 μL each of A and B reagents/ml PBS with 0.4% Triton-X 100, ABC Elite Kit, Vector Laboratories, Burlingame, CA, USA). After rinsing in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were rinsed again in PBS and then incubated for 1 hr in avidin-biotin complex (4.5 μL each of A and B reagents/ml PBS with 0.4% Triton-X 100, ABC Elite Kit, Vector Laboratories, Burlingame, CA, USA). After rinsing in PBS ...
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bioRxiv - Neuroscience 2020Quote: ... Another set of washes in PBS (3x–5 min each) were performed and then the slides were mounted using Vectashield with DAPI (Vector Labs, H-1200).
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bioRxiv - Immunology 2020Quote: ... 3 times with PBTD and counterstaining with DAPI in 5% NGS blocking solution took place after the first wash. Coverslips (No. 1) were mounted using Vectashield (Vector Laboratories, Burlingame, California) and sealed with nail polish ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then rinsed in PBS (5 x 5 min) followed by a 1 hour incubation in biotinylated goat anti-rabbit secondary (1:500 in PBS, Vector Laboratories, BA-1000). Sections were rinsed in PBS (5 x 5 min ...
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bioRxiv - Molecular Biology 2022Quote: ... ATAC-see samples were incubated for 1 hour at room temperature protected from light with blocking buffer (1X PBS, 0.1% TX-100 and 5% normal goat serum (Vector Laboratories, S-1000-20). ds/ssDNA mouse monoclonal IgM antibody clone AC-30-10 (PROGEN ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed with PBS-TX (0.5% Triton X-100 in PBS) 6 x 20 min then blocked in 5% normal goat serum (NGS; Vector Laboratories, Burlingame, CA, USA) for 1 h before being incubated on a shaker overnight with primary antibodies (Table 1 ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were again rinsed five times in PBS and then incubated for 1 hr in avidin-biotin complex (4.5 μL each of A and B reagents/ml PBS with 0.4% Triton-X 100, ABC Elite Kit, Vector Laboratories, Burlingame, CA, USA). After rinsing in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... slides were washed in TBS + 0.001 % Triton (2 x 5 min) and incubated with an ImmPress HRP secondary antibody (Vector Laboratories, MP-7401-15) for 1 h ...
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bioRxiv - Neuroscience 2024Quote: ... slides were rinsed three times for 5 minutes with 0.4% Triton-X-100 TBS and mounted using VectaShield® Hardset ™ Antifade Mounting Medium with DAPI (Vector Laboratories, California). Slides were then left alone to set for at least 48 hours at 4° C and imaged on a Leica DMi8 Confocal Microscope.
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bioRxiv - Cancer Biology 2023Quote: ... Cell nuclei were stained with spectral DAPI in PBS for 5 min and the slides were mounted with Hard-set Vectashield mounting medium (Vector Laboratories, Burlingame, CA). Mounted slides were allowed to harden prior to scanning.
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... followed by washing 3x in PBS for 5 minutes before 60-minute incubation with a Streptavidin-conjugated Dylight 488 secondary (1:200, Vector Laboratories, SA-5488). After secondary ...
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bioRxiv - Bioengineering 2023Quote: ... Slides were washed with 1X TBS twice again for 5 minutes each and Vectashield Mounting Media (H-1200; Vector Laboratories, Newark, CA, USA) was added before coverslipping ...
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bioRxiv - Pathology 2023Quote: ... Tissue sections (5 mm) from each block were cut in a cryostat at −21 C and mounted onto Vectabond-coated slides (Vector Laboratories, Peterborough, UK), fixed in cold acetone for 10 minutes and incubated with an anti PECAM-1 rat primary antibody (BD Biosciences ...
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bioRxiv - Neuroscience 2023Quote: ... The intracellular single unit recordings were performed on the L2/L3 DRGs using the quartz microelectrode containing 5% Neurobiotin (Vector Laboratories, Burlingame, CA) in 1M potassium acetate ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Haematoxylin and Eosin (H&E) staining was performed on 5-μm sections that were prepared and stained using kit (Vector Laboratories, CA, USA) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... We washed slides twice for 5 minutes in RNase-free 1X PBS and marked the sample perimeter of each slide with an ImmEdge pen (Vector Laboratories #H-4000) in between both washes ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were washed with PBS and either stained for 5 min with 1 µg/ml DAPI (for chamber slides) or mounted using DAPI-containing Vectashield (Vector Laboratories; for coverslips). An Olympus IX70 inverted microscope with a UPlanApo 100X/1.35 oil iris objective was used for image acquisition with Micro-Manager software (v2.0) ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated for two hours with a biotinylated horse anti-mouse antibody (1:500 in PBS containing 0.5% Triton X-100, Vector Laboratories, #BA-2001-.5), followed by three PBS washes ...
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bioRxiv - Molecular Biology 2021Quote: All slides were washed three times for 5 min at RT and dried before mounting with the DAPI-containing VectaMount Permanent Mounting Medium (Vector Laboratories, Burlingame, CA, USA), and sealed with nail varnish ...
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bioRxiv - Developmental Biology 2020Quote: ... Secondary antibody was decanted and slides washed three times with PBS for 5 minutes each followed by VECTASHIELD® Antifade Mounting Medium with DAPI (Vector Labs H-1200), sealing coverslip ...
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bioRxiv - Neuroscience 2021Quote: ... sections were washed two more times for 5 min in 1x PBS and mounted with Vectashield mounting media (Vector Laboratories, # H1000, Burlingame, CA, USA). The following primary antibodies and dilutions were used ...
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bioRxiv - Biochemistry 2020Quote: ... The membranes were blocked with 5% BSA and then incubated with biotin-labeled Maackia amurensis lectin II (MAL-II biotin conjugate, Vector Laboratories, Inc., Burlingame, CA) for 1 h at room temperature ...
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bioRxiv - Microbiology 2021Quote: Paraffin-embedded tissues were sectioned at 5 µm and subjected to IHC using Vectastain Elite ABC Peroxidase (HRP) Kit (Vector Laboratories Cat # PK-6102) as previously described [40] ...
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bioRxiv - Immunology 2022Quote: Clarified cell supernatant was adjusted to a final concentration of 100 mM NaCl and then incubated with Galanthus Nivalis Lectin Agarose (Vector Laboratories, AL-1243-5), washed with 10 column volumes of 20 mM Tris ...
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bioRxiv - Cell Biology 2022Quote: ... Endogenous peroxidase was blocked for 5 mins before incubation with 2.5% Normal Goat Serum (ImmPRESS Rat kit – Vector Laboratories, San Francisco Bay Area, USA) TRU for 20 mins ...
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bioRxiv - Neuroscience 2022Quote: ... slices were rinsed again 4 times for 5 minutes in PBS and revealed using a DAB substrate kit (Vector Laboratories Cat# SK-4100, RRID:AB_2336382). Stained slices (at least 5 per region ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were washed three times with 1X PBS for 5 minutes each wash and then mounted using a DAPI-staining mounting medium (Vector Laboratories; H-1200-10). Cells were imaged using Leica DMI4000B microscope fitted with Leica TCS SPE laser-scanning confocal system with Solid State lasers (405/488/561/635 ...
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bioRxiv - Microbiology 2021Quote: ... Worms were washed in PBS three times for 5 mins and transferred to new 12 well plates with 1ml of Fluorescein labeled Aleuria Aurantia Lectin (Vector labs cat# FL-1391) and Rhodamine labeled Griffonia Simplicifolia Lectin I (Vector labs cat# RL-1102 ...
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bioRxiv - Neuroscience 2022Quote: ... antigen retrieval was performed by using either formic acid for 5 minutes at room temperature or citric acid pH=6 (Vector Laboratories; Cat# H-3300) for 15 minutes at 95 °C ...
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bioRxiv - Neuroscience 2023Quote: ... The blots were washed with 1X TBST thrice for 5 minutes and then incubated with HRP-conjugated secondary antibodies (1:4000, Vector Labs, Burlingame, CA, USA) for 1-2 hours at RT ...
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bioRxiv - Microbiology 2022Quote: ... Removal of α(2,3)-linked and α(2,6)-linked sialic acids on the cell surface was verified by staining with biotinylated Maackia amurensis lectin (MAL) I (5 μg/ml; Vector Laboratories; B-1265-1) or fluorescein-labeled Sambucus nigra lectin (SNA ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... the slides were washed three times with PBS (5 minutes each at RT) and mounted using Vectashield antifade mounting media with DAPI (Vector Laboratories, #H-1200-10). To secure the coverslips ...
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bioRxiv - Neuroscience 2024Quote: ... sections were rinsed in PBS three times for 5 min each and mounted on slides with VECTASHIELD Antifade Mounting Medium with DAPI (Vector Laboratories, H-1200-10) and coverslipped ...
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bioRxiv - Pathology 2024Quote: ... sections were stained with Flash Phalloidin™ and autofluorescence quenched for 5 min with the Vector® TrueVIEW™ autofluorescence quenching kit (Vector Laboratories), then mounted with Prolong Diamond Antifade Mountant with DAPI ...