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Citations for Vector Labs :
501 - 550 of 765 citations for 6 Hydroxymethyl 4 methoxy 5 methyl nicotinic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... then 4 times 20 min in PBS at RT before leaving brains overnight in Vectashield (Vector Laboratories H-1000-10) at 4C ...
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bioRxiv - Genomics 2023Quote: The aldehyde groups resulting in a 40 μl mix were then biotinylated using a mixture containing 4 μl of NaOAc (1M, pH 6.0) and 4 μl of Biotin (Long Arm) Hydrazide (100 mM, catalog num. SP-1100, Vector Laboratories). The 48 μl resulting mixture was next incubated for 30 minutes at 40°C ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibody incubation was completed for 48 hours at 4°C followed by incubation with biotinylated secondary antibodies (Vector Labs) for 24 hours at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... Subsequently the sections were incubated with anti-rabbit secondary antibody (1:200 dilution; 4°C; 17h.; Vector Laboratories, Burlingame, USA) followed by tertiary labelling with avidin biotin complex (1:200 ...
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bioRxiv - Neuroscience 2023Quote: ... then incubated overnight in an avidin-biotin-horseradish peroxidase complex at 4°C with 0.1% Triton X-100 (Vector Elite; Vector Laboratories). The tissue was counterstained with cresyl violet and cover slipped with dibutyl phithalate (Electron Microscopy Sciences) ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were then incubated in biotinylated goat-anti-mouse antibody for 24 hours at 4 °C in blocking solution (1:200, BA-1000, Vector Laboratories). Color reaction was performed using the Vectastain Elite ABC Kit (PK-6100 ...
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bioRxiv - Genetics 2020Quote: ... Sections were incubated with primary antibody (supplementary table S4) overnight at 4°C and with universal biotinylated horse anti-mouse/rabbit immunoglobulin G secondary antibody (Vector Laboratories) for 1 hour ...
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bioRxiv - Physiology 2021Quote: ... Ears were then gently shaken (120 rpm) at 4°C overnight in 20 μg/ml Dylight 488 strepavidin (SA-5488, Vector Laboratories) and 0.05 mg/ml DAPI (D9542 ...
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bioRxiv - Neuroscience 2021Quote: ... Primary and secondary antibodies were incubated for >36h at 4°C in wash buffer (PBS + 1% BSA) and mounted in Vectashield (Vector Labs). Antibodies used were mouse anti-abd-A (gift of Ian Duncan) ...
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bioRxiv - Physiology 2021Quote: ... Frozen bone sections were incubated for 4 min at room temperature in Alkaline Phosphatase Substrate Solution ImmPACT Vector Red (Vector Laboratories). After washing with buffer ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were then incubated overnight at 4°C with their respective primary antibodies (1%–2% BSA in TBST) followed by horseradish peroxidase IgG (Vector Laboratories)-conjugated secondary antibody (anti-rabbit and anti-mouse ...
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bioRxiv - Microbiology 2021Quote: ... ovaries were fixed in 4% PFA and then mounted in Vectashield® mounting media with DAPI counterstain (Vector Laboratories, Burlingame, CA). Ovaries were then imaged using Differential Interference Contrast on a Zeiss Inverted Observer Z1 at 100X magnification.
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bioRxiv - Neuroscience 2020Quote: ... Tissue was rinsed five times for ten minutes in PBS and then incubated overnight at 4°C in a rabbit anti-goat secondary (1:500; Vector Laboratories) diluted in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... PV staining was performed as above and then sections were incubated at 4°C overnight with byotinilated VVA (1:5000, #B-1235, RRID: AB_2336855, Vector Laboratories, USA). After rinsing ...
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bioRxiv - Cancer Biology 2022Quote: ... or CD4 (ab183685, 1:400 dilution overnight at 4 degrees).Tissues were then counterstained with biotinylated anti-rabbit secondary (Vector Labs, BA-1000 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated with primary antibody for 16-24 hrs at 4°C and then for 2 hrs at 4°C with biotinylated goat anti-rabbit IgG secondary antibody (1:800, Vector Laboratories) in 5% normal goat serum in 0.1% Triton-X-100 in TBS ...
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bioRxiv - Neuroscience 2020Quote: ... Primary and secondary antibodies were incubated for >36h at 4°C in wash buffer (PBS 1% BSA) and mounted in Vectashield (Vector Labs). Antibodies used were rabbit and guinea pig anti-Hth (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were blocked with 10% normal goat or horse serum followed by overnight incubation with primary antibody at 4°C and 30 min incubation with ImmPRESS HRP-conjugated secondary antibodies (Vector Laboratories) at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... sections were incubated with primary antibodies at 4°C overnight followed with biotinylated HRP-conjugated secondary antibody (Vector Laboratories # BA-1000) at room temperature for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: ... Then the brains were washed multiple times with PBS and PBS-T for 3-4 hours and mounted in Vectashield anti-fade mounting media (H-1000, Vector Laboratories). Morphological images were obtained using Nikon A1R MP+ microscope.
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bioRxiv - Neuroscience 2022Quote: ... primary antibody overnight at 4 °C and revealed with a biotinylated goat anti-rabbit IgG secondary antibody (1:1,000, 2 hat RT, Vector Laboratories, VECTBA1000), the VECTASTAIN ABC Elite HRP kit (Vector Laboratories ...
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bioRxiv - Molecular Biology 2024Quote: ... diluted 1:2000 in blocking solution at 4 °C overnight followed by incubation with peroxidase-linked goat anti-rabbit IgG (1:10000, # PI-1000-1, Vector Laboratories). Chemiluminescent substrate (# 34095 ...
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bioRxiv - Physiology 2024Quote: ... was incubated at 4°C overnight and a biotinylated secondary antibody followed by streptavidin-DyLight 594 (BA1000 and SA-5594, Vector Laboratories) were used for cardiomyocyte identification ...
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bioRxiv - Pathology 2023Quote: ... Table 1) were incubated overnight at 4°C and binding developed with the Vectastain ABC kit (Vector Laboratories, Burlingame, CA, USA). Nuclei were stained with hematoxylin solution and preparations were mounted in DPX ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were washed and incubated first with a Biotinylated Goat Anti-Rabbit IgG Antibody (over-night at 4°C-Vector Laboratories), and then with Alexa Fluor™ 594 streptavidin (3 hours – room temperature-Thermo Fisher) ...
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bioRxiv - Developmental Biology 2024Quote: ... The polyps were kept for at least o/n at 4°C in a Vectashield mounting medium with DAPI (Vector Laboratories) before being mounted and imaged on a Leica SP8 confocal system ...
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bioRxiv - Immunology 2023Quote: ... This was followed by incubation in with biotinylated goat anti-rabbit and donkey anti-guineapig antibodies (Table S5) for 4 h at room temperature then in avidin–biotinylated horseradish peroxidase complex (Elite ABC; 1:300; Vector Laboratories) diluted in PBS for 3 h at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... Sprouts were visualized from days 3-4 via confocal imaging after overnight incubation with FITC labeled Ulex europaeus lectin (Vector labs). Immunofluorescence imaging was performed on a Yokogawa CSU-W1 spinning disk confocal microscope with 20x ...
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bioRxiv - Cell Biology 2023Quote: ... samples were washed 4×15 min with 0.2% Tween-20 in PHEM buffer and mounted in Vectashield (Vector Laboratories, H-1000). Imaging was performed using a SP8 Leica confocal microscope with a 40 x objective with 1x digital zoom ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated with primary antibodies for 48 hours at 4°C followed by incubation with biotinylated secondary antibodies (Vector Labs) for 24 hours at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... followed by an hour in Mouse-on-Mouse (MOM) Blocking Buffer (one drop MOM IgG in 4 ml PBS-T) (Vector Labs). Following the MOM block ...
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bioRxiv - Neuroscience 2024Quote: ... was followed by incubation with the primary antibody (Table S2) at 4°C for 24 or 48h in 2% (vol/vol) serum and with the corresponding biotinylated antibody (Vector Laboratories) or alkaline-phosphatase antibody (ab6722 ...
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bioRxiv - Microbiology 2024Quote: ... The membrane proteins were separated by centrifugation at 104,000 g for 1 h at 4 °C and loaded onto a column prefilled with either 2 mL of agarose-bound WFA (Vector Laboratories) for S ...
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bioRxiv - Cancer Biology 2024Quote: ... for 1 hour and then incubated overnight with the primary antibody (Table 1) containing 4 drops/ml of Biotin Solution (of the Avidin/Biotin Blocking Kit, Vector Labs) at RT ...
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bioRxiv - Systems Biology 2024Quote: ... the sections were washed again in PBS-T and incubated in Mouse-on-Mouse (MOM) Blocking Buffer (one drop MOM IgG in 4 ml PBS-T) (Vector Labs) for another hour at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were incubated with primary antibodies for 48 h at 4°C followed by incubation with biotinylated secondary antibodies (Vector Labs) for 24 h at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated for 3 days at 4°C in avidin-biotinylated horseradish peroxidase complex (Vectastain ABC Elite kit, Vector Laboratories) in TBS ...
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bioRxiv - Bioengineering 2024Quote: ... The plates were again washed 4 times with PBS-T followed by addition of avidin D-horseradish peroxidase (HRP) (Vector Laboratories) diluted 1:1,000 in PBS-T with 1% FBS ...
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bioRxiv - Molecular Biology 2021Quote: ... each for 5 min using a 42°C hot plate) before being mounted with VECTASHIELD with DAPI (Vector Labs) onto Superfrost Plus microscopy slides (VWR) ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were washed 3×5 min in PBS and incubated in freshly prepared avidin-bHRP solution (Vector Laboratories, USA) for 30 min at RT ...
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bioRxiv - Cell Biology 2021Quote: ... The cells were blocked with DPBS containing 5% (w/v) normal goat serum (Vector Laboratories, Burlingame, CA, United States), 5% (w/v ...
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bioRxiv - Cell Biology 2021Quote: ... sectioned (5 um) and processed for immunohistochemical analysis post heat-mediated antigen retrieval at pH 6.0 (Vector Labs, USA) except sections stained with anti-SOD1 antisera that were subject to antigen retrieval at pH 9.0 (Vector Labs ...
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bioRxiv - Biophysics 2020Quote: ... 1.6 kb) or control nontelomeric DNA (noTel, 4.1 kb) using the 5’ EndTag Labeling DNA/RNA Kit (Vector Laboratories). The biotinylation of the pT270 and noTel fragments was verified using AFM imaging of DNA samples in the presence of streptavidin-coated quantum dots (strep-QDs ...
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bioRxiv - Neuroscience 2021Quote: ... Peanut Agglutinin conjugated with rhodamine was used at 1:1000 to identify cones (Vector Laboratories, catalog # RL-1072-5). Secondary antibodies ...
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bioRxiv - Immunology 2020Quote: ... coverslips were washed 5 times with 1×PBS and mounted with VECTASHIELD antifade mounting medium (Vector Laboratories, CA, USA). Nuclear intensity of at least 15 infected cells was measured for each experiment and coverslip.
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bioRxiv - Neuroscience 2021Quote: ... and 1000 mM) or 500 mM KCl in 0.1 Trizma buffer with 5 - 10% neurobiotin (Vector Labs, Birmingame, USA). They were then beveled from an initial resistance of 40 - 150 MΩ to 30 - 40 MΩ using a rotary beveller (Sutter BV-10) ...
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bioRxiv - Microbiology 2021Quote: ... Cell nuclei were labeled with Hoechst 1:10,000 for 5 minutes and coverslips were mounted using Vectashield (Vector Laboratories). Slides were imaged using a Leica DM6000 Widefield microscope.
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bioRxiv - Cell Biology 2021Quote: ... washed three times with PBS at RT for 5 min and finally mounted with VECTASHIELD mounting medium (Vector Laboratories). Primary antibodies were rabbit antibodies against DDX6 (Bethyl ...
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bioRxiv - Molecular Biology 2024Quote: ... and washed two times for 5 min in MQ before mounting with Vectashield mounting media (Vector Laboratories, #H-1000). Lipid content was quantified using Qupath version 0.2.2 ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were washed three times with PBS and then blocked with normal horse serum (NHS; 5%; Vector Laboratories) for 1h at RT or overnight at 4 °C ...