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Citations for Vector Labs :
451 - 500 of 1344 citations for Piggybac Transposable Element Derived 3 PGBD3 Antibody since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... After inactivation of endogenous peroxidases and blocking with 3% H2O2 the tissue was treated with Avidin/Biotin Blocking Kit (SP-2002 Vector Labs, USA) followed by an incubation overnight at 4°C with antiDsRed rabbit polyclonal primary antibody (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... sections were washed again (3 times in PBS/Tween, 5 minutes each) and incubated in biotinylated goat anti-rabbit (1: 200, Vector Laboratories, USA) or biotinylated horse anti-mouse (1 ...
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bioRxiv - Neuroscience 2022Quote: ... free–floating brain sections were rinsed in 0.1 M PBS (2 × 15 min) followed by a 30 min blocking in 3 % normal horse serum (Vector Laboratories, Burlingame, CA) and 0.3 % Triton X-100 in 0.1 M PBS ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then washed 3 times for 5 minutes with PBS and mounted using VECTASHIELD Antifade Mounting Medium (Vector Laboratories, H-1000) or Prolong Gold Antifade Mountant with DAPI (Thermo Fisher ...
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bioRxiv - Cell Biology 2021Quote: ... Slides were again washed 3 times with 0.1% PBST with final wash in PBS prior to developing using freshly prepared DAB substrate (Vector Labs SK-4100). Slides were rinsed with water and counterstained with Hematoxylin (RICCA Chemical Company ...
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bioRxiv - Cancer Biology 2021Quote: ... and cuticles were washed 3 times in PBSS before mounting on glass slides in Vectashield mounting media without DAPI (Vector Laboratories, Inc.). Tumours were stained overnight at 4°C with DAPI ...
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bioRxiv - Plant Biology 2022Quote: ... the slides were dehydrated in 70-90-100% ethanol series for 3 min each and counterstained with 10 µg/ml 4’,6-diamidino-2-phenylindoline (DAPI) in Vectashield antifade medium (Vector Laboratories, USA). For immunodetection of microtubules ...
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bioRxiv - Neuroscience 2022Quote: ... free-floating sections were washed with PBS 3 times and blocked in 5% normal goat serum (NGS, Vector Laboratories, S-1000-20) and 0.1% Triton X-100 (Sigma ...
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bioRxiv - Neuroscience 2022Quote: ... slices were washed for 3 times in 0.1 M PBS and mounted on glass slides using Vectashield medium containing DAPI (Vector Labs, Peterborough, UK).
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bioRxiv - Neuroscience 2024Quote: Free-floating brain sections were first washed with a series of PBS and 0.5% PBS-Triton X-100 (PBS-T) before applying a 3% normal goat serum (NGS, #1000, Vector Laboratories, Burlingame, CA) blocking solution for 1 hour at room temperature (RT) ...
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bioRxiv - Genetics 2024Quote: ... Slides were washed for 5 minutes 3 times and mounted in Vectashield plus DAPI (4’,6-diamidino-2-phenylindole) (Vector Laboratories, USA). Stained testis spreads were imaged using an Olympus delta vision or Zeiss Observer microscope using 40X or 63X objectives.
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bioRxiv - Cell Biology 2020Quote: ... The coverslips with cells were mounted on 3 cm glass bottom cell culture plates using Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA). Random fields on the coverslips were photographed using an inverted Olympus IX81 microscope (Olympus Europe Holding Gmbh ...
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bioRxiv - Cell Biology 2020Quote: ... Sections were incubated at room temperature with 3% H2O2 for 10 minutes to quench endogenous peroxidase activity and blocked by 5% normal goat serum (Vector Laboratories# S-1000) in TBST (TBS containing 0.1% Tween® 20 ...
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bioRxiv - Neuroscience 2020Quote: ... Endogenous peroxidase activity and non-specific binding were blocked by incubation with 3% hydrogen peroxide in methanol for 15 minutes followed by 5% normal goat serum (Vector Laboratories, CA, USA) with 0.1% TWEEN-20 in PBS for 1 hour ...
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bioRxiv - Neuroscience 2020Quote: ... Brain sections that contained the ventrolateral preoptic nucleus were collected and blocked in PBS containing 0.25% Triton X-100 and 3% normal donkey serum (Vector Laboratories, Burlingame, CA, USA) for 60 min at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were then washed with 0.1% PBST for 3 × 10 minutes and carefully cut into 4 quadrants and mounted on slides with DAPI Vectashield (Vector Laboratories, H-1200). For cryosections ...
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bioRxiv - Cell Biology 2021Quote: ... pelleted (400 g, 6 minutes) and resuspended in 2 to 3 drops (∼75 μL) of Vectashield Antifade Mounting Medium (Vector Laboratories, H-1000). This suspension was then mixed thoroughly by pipetting to separate clumped cells ...
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bioRxiv - Neuroscience 2020Quote: Sections were again washed 3×15 min with PBS and incubated in avidin-biotin complex solution (Vectastain Elite ABC kit, Vector Laboratories, Burlingame, CA) (2-hours ...
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bioRxiv - Pathology 2020Quote: ... cryoslides were washed 3 times in PBS and then slides were fluoromounted using Vectashield mounting medium with DAPI (Vector Laboratories, Burlingame, CA, USA).
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bioRxiv - Cell Biology 2020Quote: ... The signal was revealed using Vectastain Elite ABC-HRP kit and AEC Peroxidase (HRP) substrate 3-amino-9ethylcarbazole (VECTOR AEC Peroxidase Subtract Kit SK-4200, Vector Laboratories, Burlingame, CA). Samples were mounted using ProLong Gold antifade reagent (Life Technologies ...
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bioRxiv - Neuroscience 2020Quote: ... The slides were then rinsed in diH2O and counterstained in a 1:3 diluted solution of Nuclear fast red (Vector labs H-3403). Counterstaining was done for no longer than 3 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then washed 3 times with PBS and mounted on slides with VECTASHIELD® Vibrance™ Antifade Mounting Medium with DAPI (Vector laboratories).
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bioRxiv - Cell Biology 2024Quote: ... Peroxidase was quenched by incubating them in 3% hydrogen peroxide (H2O2) for 10 min and they were then blocked with 1% normal horse serum (Vector Laboratories, SP-2001) at room temperature for 15 min ...
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bioRxiv - Molecular Biology 2023Quote: ... In the end slides were washed 3 times with 0.1%Tween in PBS and mounted with VECTASHIELD Antifade Mounting Medium with DAPI (Vector Laboratories, H-1200-10). The images were acquired using Zeiss LSM800 confocal microscope and analyses using Fiji software 86 and visualized and deposited using OMERO 87 (project ID:12920).
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bioRxiv - Cell Biology 2023Quote: ... slides were washed in 3 x 5 min in TBST and mounted with Vectashield® mounting medium (Cat. No. H-1000; Vector Laboratories, USA).
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bioRxiv - Neuroscience 2024Quote: ... for 30 seconds to block endogenous fluorescence signal before washing in PBS (3 x 2 mins) and cover slipped and mounting with Vectashield antifade mounting medium with DAPI (H1900, Vector Laboratories, Peterborough, UK).
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bioRxiv - Neuroscience 2024Quote: ... slides were washed with PBS and incubated for at least an hour with blocking buffer containing 3% normal goat serum (NGS; Vector Laboratories, Burlingame, CA) and 0.4% Triton X-100 (Sigma Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... The proximal end of the femur was first dipped in phosphate-buffered saline with 0.5% Triton-X (PBST) for 2-3 seconds and immediately transferred to a Vaseline® well of neurobiotin 488 tracer (Vector Labs, USA) solution prepared in 1M NaCl ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were washed 3 times with PBST for 5 min each and mounted with Vectashield vibrance with DAPI (Cat. # H-1800-10, Vector Labs, Newark, CA). Cells were imaged at 63x on the confocal 60 μm disk setting with the BioTek Cytation C10 using the Gen5 software (Agilent) ...
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bioRxiv - Cell Biology 2024Quote: ... and after 3 more washes with PBS the coverslips were mounted with Vectashield solution (mounting medium with DAPI, Vector Laboratories Inc., Burlingame, CA). Evaluation was done using a Nikon 80i or Bio-rad confocal microscope ...
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bioRxiv - Cancer Biology 2021Quote: ... incubated with the corresponding biotin-conjugated secondary antibodies (Vector Laboratories, Burlingame, CA) at room temperature for one hour ...
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bioRxiv - Cancer Biology 2020Quote: ... biotinylated anti-rabbit secondary antibody (Vector Laboratories, Cat. BA-1000; 1:50) and Vectastain® ABC kit (Vector laboratories) ...
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bioRxiv - Cancer Biology 2020Quote: ... biotinylated anti-rabbit secondary antibody (Vector Laboratories, Cat. BA-1000; 1:50) and Vectastain® ABC kit (Vector laboratories) ...
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bioRxiv - Cancer Biology 2020Quote: ... the sections were incubated with ImmPRESS anti-rat secondary antibody (Vector Laboratories) or with Dako Envision system-HRP labeled polymer anti-rabbit antibodies (Dako ...
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bioRxiv - Physiology 2020Quote: ... the secondary antibody was conjugated with horseradish peroxidase (HRP) from Vector Labs (Vectastain ABC HRP kit ...
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bioRxiv - Neuroscience 2020Quote: ... For immunohistochemistry the following biotinylated antibodies were used from Vector Laboratories (UK): biotinylated anti-rabbit IgG (H+L ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were incubated with rabbit or rat biotinylated secondary antibodies (Vector Laboratories) and conjugated with an ABC-HRP biotin/avidin complex kit (Vector Laboratories) ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were incubated in biotinylated secondary antibodies (1:1000; Vector Laboratories, USA) for two hours at room temperature.
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bioRxiv - Neuroscience 2020Quote: ... biotinylated secondary antibody goat anti-mouse (1:200, Vector Laboratories, Burlingame, USA) or goat anti-rabbit (1:200 ...
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bioRxiv - Microbiology 2020Quote: ... The secondary antibody is an anti-rabbit IgG polymer from Vector Laboratories ImPress VR ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were incubated in biotinylated secondary antibodies (1:1000; Vector Laboratories, USA) for two hours at room temperature ...
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bioRxiv - Pathology 2021Quote: ... incubated with biotin-labelled goat-anti-rabbit IgG secondary antibody (Vector Labs) for 20min ...
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bioRxiv - Cancer Biology 2021Quote: ... Peroxidase labeled anti-rabbit secondary antibody (PI-1000, Vector laboratories, Burlingame, CA) was diluted 1:5000 in 5% non-fat powdered milk and 1X TBST ...
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Pseudohypoxic HIF pathway activation dysregulates collagen structure-function in human lung fibrosisbioRxiv - Cell Biology 2021Quote: ... followed by a biotinylated secondary antibody (1:500, Vector Laboratories Ltd., UK); antibody binding was detected using streptavidin-conjugated horse-radish peroxidase and visualised using DAB before counter-staining with Gill’s Haematoxylin ...
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bioRxiv - Cancer Biology 2022Quote: ... Antigen-antibody binding was detected by Elite kit (PK-6100, Vector Laboratories) and DAB (DAKO ...
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bioRxiv - Cancer Biology 2022Quote: ... Primary antibody diluted in dilution buffer (PBS, 1%BSA, Biotin (Vector Laboratories)) was added and incubated in a humidified chamber ...
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bioRxiv - Cancer Biology 2022Quote: ... antibody staining was visualized with Vectastain Elite ABC immunoperoxidase system (Vector Laboratories), and sections were counterstained with hematoxylin ...
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bioRxiv - Neuroscience 2021Quote: ... then successively incubated with Amplifier Antibody and ImmPRESS Polymer Reagent (Vector labs) and the ImmPACT DAB EqV working solution (Vector labs ...
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bioRxiv - Neuroscience 2020Quote: ... antibodies and developed with biotinylated secondary anti-rabbit (1:500; Vector Laboratories) and avidin-biotin-peroxidase complex (ABC Elite Kit ...
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bioRxiv - Molecular Biology 2021Quote: ... and rabbit anti-sheep FITC antibodies (FI-6000, Vector Laboratories, RRID: AB_2336218). Widefield fluorescence imaging was performed on a DeltaVision Elite system (Applied Precision ...