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Citations for Vector Labs :
451 - 500 of 504 citations for N6 2 aminoethyl 9H purine 2 6 diamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... Migrated cells located at the bottom side of the transwell membrane were permeabilized with 0.1% Triton X-100 in PBS and the transwell membrane was coverslipped with mounting medium containing DAPI (Vector Labs, Cat # H-1800-2). For each transwell ...
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bioRxiv - Immunology 2023Quote: ... The brain lysate was 1:10 diluted in the PBS and stained with rhodamine-labeled dolichos biflorus agglutinin (DBA-red, Vector Laboratories, RL-1032-2). GFP and DBA-red double-positive cysts were counted and diluted to 5 cysts per 200 uL of PBS ...
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bioRxiv - Microbiology 2023Quote: ... the slides were stained with 4’,6-diamidino-2-phenylindole (DAPI) and mounted in a 4:1 mixture of Citifluor (Citifluor Ltd, London, UK) and Vecta Shield (Vector Laboratories, Inc., Burlingame, CA), covered with glass coverslips ...
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bioRxiv - Neuroscience 2023Quote: ... Incubation in primary antibody was followed by washes and a 2-hour incubation in anti- rabbit biotinylated secondary antibodies (1:200, Vector Laboratories, Burlingame, CA, RRID:AB_2313606). Sections were then washed and incubated for 1.5 hours in avidin- biotin complex solution (1:200 ...
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bioRxiv - Genetics 2024Quote: ... or Cy5 (1:1000; USBIO 518482) conjugated Lotus Tetragonolobus Lectin (LTA) and Rhodamine conjugated Dolichos Biflorus Agglutinin (DBA) (1:1000; Vector Laboratories RL-1032-2), was added to co-stain proximal tubules and collecting ducts ...
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bioRxiv - Neuroscience 2023Quote: ... The supernatant was incubated at least 2 hr with 30 μl package volume of Wheat-germ (WGA) -conjugated agarose beads (Vector Laboratories, Inc., Burlingame, CA) on a rotating mixer at 4°C and washed 3 times with lysis buffer ...
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bioRxiv - Synthetic Biology 2024Quote: ... glasses with the labeled cells were placed onto the Superfrost Plus adhesion slides (Epredia, EPBRSF41296SP) in 20 µl VECTASHIELD Vibrance Antifade Mounting Media (Vector Laboratories, H-1700-2) and stored at +4℃ in the dark.
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bioRxiv - Microbiology 2023Quote: ... Parasites were then stained with an anti-VSG-2 monoclonal antibody (Hovel-Miner et al., 2012) conjugated to Alexa647 and mounted in Vectashield (Vector Laboratories, H-1800-10) containing 4′,6-diamidino-2-phenylindole (DAPI) ...
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bioRxiv - Neuroscience 2023Quote: ... free-floating sections were first washed 3 × 10 min in 1x PBS and then incubated for 30 min in a blocking solution of 2% normal goat serum (S-1000-20; Vector Laboratories, Newark, CA, USA) and 0.1% Triton x-100 (T8787-50ML ...
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bioRxiv - Cell Biology 2023Quote: ... cells stained with DAPI followed by wash in 2x SSC buffer before being mounted using VECTASHIELD Vibrance Antifade Mounting Medium (Vector Laboratories H-1700-2).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and incubated for 2-3 hours with biotinylated anti-rabbit secondary antibody (1:450 for DeltaFosB and 1:600 for c-Fos, Vector Laboratories, Burlingame, CA, USA). Finally ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Samples were washed in PBSTr 0.2% at RT (8 x 15 min) and infiltrated with VECTASHIELD PLUS Antifade Mounting Medium (Vector Laboratories, cat. H-1900-2).
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated overnight with agitation at 4°C with Wisteria floribunda lectin (WFA, WFL, Biotinylated; Vector Laboratories, Newark, CA, B-1355-2) diluted in staining buffer (2% PBST and 2% NGS ...
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bioRxiv - Neuroscience 2024Quote: ... for 30 min, a first antibody solution (PB 0.1M, Triton 0.3%, NGS 2%, Rabbit α-Phal-L 1:8000 (Vector Laboratories, AS-2300, RRID: AB_2313686), Mouse α-PV 1:4000 (Swant ...
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bioRxiv - Microbiology 2024Quote: ... washed twice with PBS and blocked for ∼10 minutes in 2% BSA (Fisher, cat: 9048-46-8) in PBS (w/v) while the click chemistry (CC) labeling reaction (Vector labs, cat: CCT-1317) was prepared ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and DAB chromogen with nickel (Vector Labs SK-4100; 6 min). After a distilled water wash ...
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bioRxiv - Cancer Biology 2021Quote: ... After a 5 min wash with PBS the slide was incubated for 30 min with the Vectastain Elite ABC reagent followed by a 2-10 min incubation with a DAB peroxidase substrate solution (Vector Laboratories, Cat. No. SK-400) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... immunofluorescence was performed on paraffin sections using antibodies in Supplementary Table 2 and the following reagents: ImmPRESS® HRP Horse Anti-Rabbit IgG Polymer Detection Kit (Vector Labs, MP-7401-50). Following the application of TSA fluorophores (listed in Supplementary Table 2 ...
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Analysis of spike protein variants evolved in a novel mouse model of persistent SARS-CoV-2 infectionbioRxiv - Microbiology 2023Quote: ... The slides stained with rabbit anti-SARS-CoV-2 S polyclonal antibody or rabbit anti-ACE2 antibody were further incubated with biotinylated horse anti-rabbit antibody (Vector Laboratories, cat. no. BP 1100). All slides were then incubated with HRP-conjugated streptavidin (Vector Laboratories ...
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bioRxiv - Cell Biology 2020Quote: ... 6-diamidinophenylindole (DAPI) in Vectashield anti-fade medium (Vector Laboratories, CA, USA).
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bioRxiv - Pathology 2023Quote: ... mounted using 4′,6-diamidino-2phenylindole (DAPI: Vector Laboratories, Burlingame, CA, USA), and examined employing a laser confocal microscope (LSM 710 ...
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bioRxiv - Bioengineering 2024Quote: ... 6 µL of DAPI-containing mounting media (Vector Laboratories, Vectashield #H-1200) was added to the surface of each scaffold ...
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bioRxiv - Genetics 2022Quote: ... and either directly seeded on 10cm cell culture dishes or further enriched with Lotus Tetragonolobus Lectin (LTL; Vector labs biotynilated beads, cat. no. B-1325-2) LTL or Dolichos Biflorus Agglutinin (DBA ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated overnight at 4°C in slow agitation with primary antibody and/or biotinylated Wisteria Floribunda lectin (WFA, 1:1000, Vector Labs, USA, Ref. B-1355-2). The following primary antibodies were used ...
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bioRxiv - Neuroscience 2024Quote: ... sections were rinsed with 0.1 M PB (4 x 5 min) and incubated in biotinylated goat anti-mouse IgG antibody (Vector Laboratories: 1/200 in 2% NGS in 0.1M PB) for 30 min ...
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bioRxiv - Cancer Biology 2024Quote: ... antigen retrieval using Antigen Unmasking Solution Citrate-Based pH 6 (Vector Laboratories #H-3300) with 0.05% Tween 20 (Biorad #1610781 ...
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bioRxiv - Developmental Biology 2023Quote: ... the antigenic sites were unmasked with a citrate buffer (pH 6; H-3300, Vector Laboratories), and endogenous peroxidases were blocked with a 3% H2O2 solution (H1009 ...
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bioRxiv - Immunology 2022Quote: ... Heat-mediated antigen retrieval followed with a pH 6 unmasking solution (Vector Laboratories, H-3300) and a pH 9 unmasking solution (Abcam 100X Tris-EDTA) ...
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bioRxiv - Bioengineering 2022Quote: ... After rehydration, antigen retrieval was conducted using Unmasking solution (Citrate based, pH 6) (Vector Laboratories, H-3300) at 95 °C for 30 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... Coverslips were then mounted and counterstained on 6 μl of VectaShield mounting agent with DAPI (Vector Lab, H1200), and sealed before being imaged on Nikon’s Inverted Ti eclipse equipped with an Andor Zyla sCMOS camera (2560 × 2160 ...
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bioRxiv - Cancer Biology 2020Quote: FFPE sections (4um) were deparaffinised and used for heat mediated antigen retrieval (citrate buffer pH 6, Vector labs). Sections were blocked for 10min in 3%H2O2 ...
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bioRxiv - Neuroscience 2020Quote: ... each slice was incubated for thirty-five minutes with 6 mg/mL DyLight 594 Isolectin B4 conjugate (Vector Labs) to label vascular endothelium as previously described(18).
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bioRxiv - Neuroscience 2020Quote: ... Samples were incubated overnight with Dolichos biflorus lectin conjugated to Tetramethylrhodamine-5-(and 6)-isothiocyanate (DBA-TRITC, Vector Lab) at 10 μg/mL diluted in blocking buffer at 4°C ...
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bioRxiv - Physiology 2020Quote: ... Samples were then rinsed 6 times with PBSTx.3B at room temperature and mounted in Vectashield® (Vector Laboratories) for subsequent imaging ...
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bioRxiv - Neuroscience 2022Quote: ... The brains were then washed in PBS (6 x 10 min each) and mounted in Vectashield (Vector Laboratories Inc) on a cover slip ...
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bioRxiv - Neuroscience 2022Quote: ... Slides were washed 6 times for 20 min in PBS-T and mounted in Vectashield with DAPI (Vector Laboratories).
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bioRxiv - Cell Biology 2020Quote: ... epitopes were retrieved by immersing slides through 35 min incubation at 95°C in citrate buffer (Vector Laboratories, pH 6) or Tris-EDTA buffer (Cellgro ...
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bioRxiv - Neuroscience 2020Quote: The next day sections were wash 6 × 10 min with wash buffer (1 × TBS, 0.05% triton X-100) and incubated with biotinylated antibody (Vector Laboratories) diluted 1:200 in secondary blocking buffer (1 × TBS pH 7.6 ...
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bioRxiv - Cancer Biology 2022Quote: ... Antigen retrieval was performed using heat-mediated microwave methods and the antigen unmasking solution (Vector Laboratories, H-3300 pH 6) was used for cleaved caspase 3 ...
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bioRxiv - Molecular Biology 2024Quote: ... Sections (6 μm) were deparaffinized and rehydrated in a graded alcohol series followed by antigen retrieval (Vector Laboratories, Burlingame, CA) and hydrogen peroxide treatment ...
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bioRxiv - Neuroscience 2024Quote: ... β-amyloid was detected using thiazine red (1 µM, Cat# 2150-33-6, Chemsavers) associated with DAPI (0.5 µM, Cat# 1485, Vector lab) and a mouse monoclonal antibody against VSM α-smooth muscle actin (Cat# ABT1487 ...
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bioRxiv - Microbiology 2024Quote: ... and antigens were exposed by incubating slides in Vector Labs Antigen Unmasking Solution Citrate Buffer pH 6 (Vector labs, #F4680) in a steamer for 30 min ...
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bioRxiv - Cell Biology 2022Quote: ... Intestine sections were rehydrated and antigens were unmasked for 30 min in a steamer with sodium citrate (10 mM, pH 6) (Ki67, GFP, p57, PCNA, cleaved caspase 3) or High pH (H-3301, Vector Laboratories) (Olfm4 ...
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bioRxiv - Microbiology 2022Quote: ... The membrane was washed 6 separate times in TBS-T for 5 min and then incubated with HRP-conjugated streptavidin (Vector Laboratories) for an additional hour ...
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bioRxiv - Neuroscience 2024Quote: ... (recognizing Aβ and C99 but not holo-APP) or for 30 min in citric acid solution pH=6 at 90-100°C (Pressure cooker, Vector Laboratories) for 82E1 and anti-Iba1 (1/1,000 ...
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bioRxiv - Neuroscience 2024Quote: ... the brains were mounted on a microscope slide within a 6 µL drop of VECTASHIELD Mounting Medium (Vector Laboratories, H-1000) set within a reinforcement ring ...
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bioRxiv - Cancer Biology 2024Quote: ... the sections were first rinsed with PBS for 15 minutes and then treated with a citrate-based antigen unmasking solution (10 mM sodium citrate, pH 6, Vector Labs) that was heated to 95C for 20 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... and 6 sections with distal CA1 TdTomato+ dendrites were mounted on slides and coverslipped using Vectashield Hardset mounting media (Vector Laboratories #H140010).
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bioRxiv - Neuroscience 2022Quote: ... Sections were blocked for 1 h in 0.3% PBS-Triton-X-100 with 6% normal goat serum (NGS; Vector Labs, S-1000), followed by incubation for 72 h at 4 ºC with anti-cFos rabbit primary antibody (1:2000 ...
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bioRxiv - Neuroscience 2022Quote: ... Antigen retrieval was performed by microwaving the samples for 15 minutes in 0.01 M citrate buffer (pH = 6, Vector Laboratories, H-3300) in Millipore water ...