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Citations for Vector Labs :
451 - 500 of 991 citations for 4 5 5 5 Tetrafluoro 4 trifluoromethoxy pentan 2 one since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... sections were washed again (3 times in PBS/Tween, 5 minutes each) and incubated in biotinylated goat anti-rabbit (1: 200, Vector Laboratories, USA) or biotinylated horse anti-mouse (1 ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were permeabilized with 0.5% Triton X-100 for 30 min at room temperature and blocked in 5% goat serum (Vector Laboratories, 1:200), goat anti-rat 488 and goat anti-mouse 647 (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were then washed 3 times for 5 minutes with PBS and mounted using VECTASHIELD Antifade Mounting Medium (Vector Laboratories, H-1000) or Prolong Gold Antifade Mountant with DAPI (Thermo Fisher ...
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bioRxiv - Physiology 2020Quote: ... Sections were washed with PBS for 10 min before staining with 1:1000 dilution of rhodamine wheat germ agglutinin (WGA; 5 μg/mL;Vector Laboratories,UK) for 10 min ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were washed 5 times again and mounted onto slides using Vectashield Hardset Antifade reagent with DAPI (Vector Labs, cat. # H-1400). Thalamocortical slice imaging was performed on an Andor Dragonfly microscope described below.
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bioRxiv - Molecular Biology 2021Quote: ... Sections were washed with PBS for 10 min before staining with 1:1000 dilution of rhodamine wheat germ agglutinin (WGA; 5 μg/mL;Vector Laboratories, UK) for 10 min ...
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bioRxiv - Neuroscience 2022Quote: ... or Phaseolus vulgaris leucoagglutinin (PHA-L; 2.5% solution in 10 mM phosphate buffer, pH 7.4; Vector Laboratories, Cat. No. L-1110-5). To reduce the number of animals ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were washed 4 × 10min in TBS 0.1% Tween and incubated with one of the following peroxidase-conjugated secondary antibodies diluted in TBS 0.1% Tween supplemented with 5%BSA for 2h at room temperature: horse anti-mouse IgG(H+L) (Vector laboratories, PI-2000), goat anti-rabbit IgG(H+L ...
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bioRxiv - Neuroscience 2023Quote: ... slides were deparaffinized and boiled for 20 min in citrate buffer (0.1M sodium citrate and 0.1M citric acid in distilled H2O) for antigen retrieval and incubated with 5% normal rabbit serum (Vector laboratory #S-5000) in PBS containing 0.2% Triton for 1 h at RT to block unspecific binding ...
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bioRxiv - Neuroscience 2022Quote: ... free-floating sections were washed with PBS 3 times and blocked in 5% normal goat serum (NGS, Vector Laboratories, S-1000-20) and 0.1% Triton X-100 (Sigma ...
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bioRxiv - Developmental Biology 2024Quote: ... Slides were washed twice in PBS for 5 min and mounted on a drop of VECTASHIELD solution (Vector Laboratories, H-1200-10). Images were acquired using a Zeiss LSM 880 confocal microscope.
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bioRxiv - Genetics 2024Quote: ... Cover slips were subsequently washed with Wash buffer B for 5 minutes and mounted onto the slides with Vectashield® Mounting Medium (H-1000, Vector Laboratories). Images were taken at the Imaging Facility ...
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bioRxiv - Microbiology 2024Quote: ... the specimens were washed in PBS for 5 min each and cover with glass slides using mounting medium (Vector Laboratories, United States). For imaging ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were blocked/permeabilized for 1 hour at room temperature with a solution of 5% Normal Horse Serum (Vector Laboratories, Burlingame, California), 0.2% Triton X-100 (Amresco ...
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bioRxiv - Neuroscience 2023Quote: ... we removed the supernatant and added 1 ml ACSF with DAPI (Vector Laboratories, Cat No.: H-1200-10ml, 5 μ g/ml). Collected single-cell suspensions were used for library construction.
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bioRxiv - Neuroscience 2023Quote: ... The sections were incubated at 4 °C overnight with primary antibody diluted in PBS containing 0.3% Triton X-100 and 5% normal goat serum blocking solution (Vector Laboratories, #S-1000). Anti-mouse monoclonal pS202/pT205 1:1000 (AT8 ...
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bioRxiv - Cancer Biology 2024Quote: ... Sections were washed with 1x PBS for 5 min and antigen retrieval was performed with Antigen Unmasking Solution Citrate-based pH 6.0 (Vector Laboratories H-3300) in a Decloaking Chamber™ (Biocare Medical ...
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bioRxiv - Cell Biology 2024Quote: ... After blocking with 5% goat serum/1% bovine serum albumin/PBS and Mouse on Mouse blocking reagent (Vector Laboratories, Newark, CA, USA), the samples were incubated with primary antibodies overnight at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: Immunohistochemistry on 5 µm sections from frozen or paralin-embedded tissues was performed using the biotin-peroxidase method (Vector laboratories, Burlingame, CA). IHC staining and scoring were evaluated by a trained pathologist.
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bioRxiv - Neuroscience 2024Quote: ... followed by washing in ultrapure water for 5 min and mounted with Vectashield Vibrance® Antifade Mounting Medium (# ZKO8O3, Vector Labs, US) with or without DAPI (0.001 mg/ml) ...
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bioRxiv - Cancer Biology 2024Quote: ... The incubation with the primary antibody is done for 5 hours followed by biotinylated mouse secondary (Vector Labs, MOM Kit BMK-2202) in 5.75μg/mL ...
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bioRxiv - Physiology 2024Quote: ... Cardiac sections were then washed in PBST and labeled with FITC conjugated Wheat-Germ Agglutinin (1:500; Vector Labs #FL-1021-5) and Goat anti-Mouse IgM Alexa Fluor 594 (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... tissue was rinsed again three times (5 minutes each) in 0.01M PBS before immersing in an avidin-biotin complex (ABC; Vector labs, PK-4000) in the same concentration PBS for 30 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... and incubated with 4 μg/mL biotinylated WGA (Vector Laboratories) diluted in Carbo-Free Blocking Solution ...
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bioRxiv - Neuroscience 2021Quote: ... Electrode tips were then filled with 4% neurobiotin (Vector Laboratories) in 1 M KCl ...
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bioRxiv - Cancer Biology 2021Quote: ... 100 uL of 4 μg/mL biotinylated AAL (Vector Laboratories) was added and incubated at RT for 90 min ...
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bioRxiv - Genetics 2021Quote: ... the gonads were again washed three times with PBSTw and resuspended in 1 drop of Vectashield containing 4’,6-diamidino-2-phenylindole (DAPI) (Vector Laboratories, Burlingame, CA). The gonad was finally mounted on pads of 5% agarose in water on microscope slides and covered with a cover glass (Sulston and Horvitz ...
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bioRxiv - Neuroscience 2021Quote: ... The slices were then washed three times with PBS and mounted with 4’,6-diamidino-2-phenylindole (DAPI)-containing Vectashield (Vector Laboratories, CA, USA). Cell counting was done manually on brain sections containing the mPFC ...
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bioRxiv - Developmental Biology 2021Quote: ... embryos were incubated in the second antibody solution for 1 h at 37° C and subsequently mounted in Vectashield mounting medium containing 4′,6-diamidin-2-phenylindol (DAPI, Vector Laboratories, Burlingame, California) in a manner that conserves the 3D structure of the specimen [42] ...
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bioRxiv - Neuroscience 2023Quote: ... for 40 seconds and sections were finally coverslipped with Vectashield mounting medium containing 4′,6-diamidino-2-phenylindole (DAPI) to label the nucleus (Vector Laboratories, H-1800). Slides were kept at 4 °C until imaging ...
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bioRxiv - Physiology 2023Quote: ... the sections were mounted with hard-set DAPI (4′, 6-diamidino-2-phenylindole –hard set; Cat# H-1500, Vector Laboratories Ltd., Peterborough, UK). The slides were left overnight in the dark at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... staining was 2% goat serumPrimary antibodies were left overnight in 4 degrees and followed by anti-rabbit secondary (Vector Laboratories Cat# BA-1000). Next ...
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bioRxiv - Physiology 2023Quote: ... The coverslips were washed in PBS before being mounted onto a microscope slide with 4’,6-diamidino-2-phenylindole (DAPI) (Vectashield, Vector Laboratories, Burlingame, CA) mounting medium.
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bioRxiv - Bioengineering 2022Quote: ... Secondary antibodies were incubated with the sections for one hour before washing using PBS and mounting using Vectashield Antifade Mounting Medium with 4’,6-diamidino-2-phenylindole (DAPI) (Vector Laboratories, Burlingame, CA).
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bioRxiv - Neuroscience 2022Quote: ... Cells incubated with MitoSOX™ were then fixed with 3.7% paraformaldehyde and applied with an anti-fading mounting medium with 4′,6-diamidino-2-phenylindole (DAPI; H-1200, Vector Laboratories, Burlingame, CA). Live or fixed cell images were captured at an excitation/emission (in nm ...
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bioRxiv - Neuroscience 2023Quote: ... slices were incubated overnight at 4°C with a solution containing biotinylated Wisteria floribunda Lectin (WFA, B-1355-2, Vector Laboratories, 1:200) and 3% BSA in PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... The sections were washed with PBS and mounted using VECTASHIELD® Vibrance™ mounting medium with 4′,6-diamidino-2-phenylindole (DAPI, Vector Laboratories). Table S6 lists the primary and secondary antibody dilutions for the organ tissues ...
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bioRxiv - Neuroscience 2024Quote: ... Slides were then cover slipped with Vectashield mounting medium with 4′,6-diamidino-2-phenylindole (DAPI) for nuclear staining (Vector Laboratories, H-1800) and kept at 4L°C until imaging ...
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bioRxiv - Neuroscience 2024Quote: ... and the cover-slips applied with a drop of Vectashield-containing DAPI (4’,6-diamidino-2-phenylinodole; catalog number: H-1200; Vector Laboratories, Burlingame, CA). Sections were visualized on a Zeiss AxioImager M2 microscope equipped with an AxioCam MRm and the ApoTome imaging system ...
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bioRxiv - Immunology 2024Quote: ... diluted in 0.1% Triton X-100 in PBS and then mounted with Vectashield Mounting Medium containing 4′,6-diamidino-2-phenylindole (DAPI, Vector Laboratories, Burlingame, CA). Confocal microscopy (performed on a Leica TCS SP8 or Leica Stellaris 8 ...
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bioRxiv - Neuroscience 2024Quote: ... slides were incubated O/N at 4 °C with biotinylated Wisteria Floribunda Agglutinin (WFA, Vector Laboratories, CA, USA; #B-1355-2 1:2000). Afterward ...
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bioRxiv - Cell Biology 2020Quote: ... Sections were incubated at room temperature with 3% H2O2 for 10 minutes to quench endogenous peroxidase activity and blocked by 5% normal goat serum (Vector Laboratories# S-1000) in TBST (TBS containing 0.1% Tween® 20 ...
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bioRxiv - Neuroscience 2020Quote: ... Endogenous peroxidase activity and non-specific binding were blocked by incubation with 3% hydrogen peroxide in methanol for 15 minutes followed by 5% normal goat serum (Vector Laboratories, CA, USA) with 0.1% TWEEN-20 in PBS for 1 hour ...
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bioRxiv - Neuroscience 2021Quote: ... incubated in 0.1% Triton X-100 in PBS for 10 minutes at room temperature and blocked in 5% normal goat serum (NGS) (Vector Labs, Burlingame, CA) with 0.1% Triton X-100 ...
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bioRxiv - Synthetic Biology 2021Quote: ... blocked in 5% bovine serum albumin for 30 min and probed for 1 h with fluorescein-labeled SBA (Vector Laboratories, catalog # FL-1011) and Alexa Fluor 647® (AF647)-conjugated anti-His antibody (R&D Systems ...
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bioRxiv - Cell Biology 2021Quote: ... the retinal sections were washed with PBS three times for 5 min each and then mounted with Vectashield Mounting Medium (Vector Laboratories, Burlingame, CA). The images were acquired with a BZ-X810 Keyence microscope (Keyence ...
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bioRxiv - Physiology 2021Quote: ... Sections were incubated at room temperature with 3% (v/v) H2O2 for 10 min to quench endogenous peroxidase activity and blocked by 5% (v/v) normal goat serum (Vector Laboratories, #S-1000) in PBS for 1 hour ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were rinsed again in PBS and then incubated for 1 hr in avidin-biotin complex (4.5 μL each of A and B reagents/ml PBS with 0.4% Triton-X 100, ABC Elite Kit, Vector Laboratories, Burlingame, CA, USA). After rinsing in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were rinsed again in PBS and then incubated for 1 hr in avidin-biotin complex (4.5 μL each of A and B reagents/ml PBS with 0.4% Triton-X 100, ABC Elite Kit, Vector Laboratories, Burlingame, CA, USA). After rinsing in PBS ...
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bioRxiv - Neuroscience 2020Quote: ... Another set of washes in PBS (3x–5 min each) were performed and then the slides were mounted using Vectashield with DAPI (Vector Labs, H-1200).