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Citations for Vector Labs :
451 - 497 of 497 citations for 2 Pyridin 2 yl acetic acid d4 hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Parasites were then stained with an anti-VSG-2 monoclonal antibody (Hovel-Miner et al., 2012) conjugated to Alexa647 and mounted in Vectashield (Vector Laboratories, H-1800-10) containing 4′,6-diamidino-2-phenylindole (DAPI) ...
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bioRxiv - Neuroscience 2023Quote: ... Incubation in primary antibody was followed by washes and a 2-hour incubation in anti- rabbit biotinylated secondary antibodies (1:200, Vector Laboratories, Burlingame, CA, RRID:AB_2313606). Sections were then washed and incubated for 1.5 hours in avidin- biotin complex solution (1:200 ...
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bioRxiv - Genetics 2024Quote: ... or Cy5 (1:1000; USBIO 518482) conjugated Lotus Tetragonolobus Lectin (LTA) and Rhodamine conjugated Dolichos Biflorus Agglutinin (DBA) (1:1000; Vector Laboratories RL-1032-2), was added to co-stain proximal tubules and collecting ducts ...
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bioRxiv - Immunology 2023Quote: ... The brain lysate was 1:10 diluted in the PBS and stained with rhodamine-labeled dolichos biflorus agglutinin (DBA-red, Vector Laboratories, RL-1032-2). GFP and DBA-red double-positive cysts were counted and diluted to 5 cysts per 200 uL of PBS ...
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bioRxiv - Neuroscience 2023Quote: For light and electron microscopic analysis when we visualized the L5 fibers in the brainstem after the anti-mCherry primary antibody we used anti-rabbit ImmPRESS (1:2, Vector Laboratories Burlingame, Ca 94010) and DAB-Ni/DAB (DAB-Ni ...
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bioRxiv - Bioengineering 2023Quote: ... Migrated cells located at the bottom side of the transwell membrane were permeabilized with 0.1% Triton X-100 in PBS and the transwell membrane was coverslipped with mounting medium containing DAPI (Vector Labs, Cat # H-1800-2). For each transwell ...
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bioRxiv - Pathology 2023Quote: ... The tissue sections were washed and mounted on microscope slides with VECTASHIELD Antifade Mounting Medium with 4′,6′[Diamidino[2[phenylindole (DAPI; H-1200, Vector Laboratories, Newark, CA, USA) to preserve fluorescence and nuclear staining ...
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bioRxiv - Microbiology 2023Quote: ... the slides were stained with 4’,6-diamidino-2-phenylindole (DAPI) and mounted in a 4:1 mixture of Citifluor (Citifluor Ltd, London, UK) and Vecta Shield (Vector Laboratories, Inc., Burlingame, CA), covered with glass coverslips ...
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bioRxiv - Developmental Biology 2023Quote: ... Nuclei were counterstained with 4’,6-diamidino-2-phenyl-indole (DAPI) diluted at 10 μg/ml in the mounting medium (Vectashield; Vector Laboratories, Newark, CA, USA). ImmPRESS® Polymer Detection Kits MP-7500 and MP-7405 were used according to the manufacturer’s protocol (Vector Laboratories).
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bioRxiv - Cell Biology 2023Quote: ... cells stained with DAPI followed by wash in 2x SSC buffer before being mounted using VECTASHIELD Vibrance Antifade Mounting Medium (Vector Laboratories H-1700-2).
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bioRxiv - Neuroscience 2023Quote: ... The supernatant was incubated at least 2 hr with 30 μl package volume of Wheat-germ (WGA) -conjugated agarose beads (Vector Laboratories, Inc., Burlingame, CA) on a rotating mixer at 4°C and washed 3 times with lysis buffer ...
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bioRxiv - Neuroscience 2024Quote: ... for 30 min, a first antibody solution (PB 0.1M, Triton 0.3%, NGS 2%, Rabbit α-Phal-L 1:8000 (Vector Laboratories, AS-2300, RRID: AB_2313686), Mouse α-PV 1:4000 (Swant ...
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bioRxiv - Neuroscience 2024Quote: ... Cover slips were added and sealed with Vectashield mounting medium plus 4’,6-diamindino-2-phenylindole (DAPI) to visualise nuclei (Vector Laboratories, Burlingame CA, USA). Slides were imaged with an upright confocal microscope (Zeiss LSM880)
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bioRxiv - Microbiology 2024Quote: ... washed twice with PBS and blocked for ∼10 minutes in 2% BSA (Fisher, cat: 9048-46-8) in PBS (w/v) while the click chemistry (CC) labeling reaction (Vector labs, cat: CCT-1317) was prepared ...
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bioRxiv - Neuroscience 2024Quote: ... sections were washed and mounted on slides using a Vectashield mounting medium with 4′,6-diamidino-2-phenyl indole (DAPI) (H-1200; Vector Laboratories, Inc., CA, USA) and a coverslip ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and incubated for 2-3 hours with biotinylated anti-rabbit secondary antibody (1:450 for DeltaFosB and 1:600 for c-Fos, Vector Laboratories, Burlingame, CA, USA). Finally ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Samples were washed in PBSTr 0.2% at RT (8 x 15 min) and infiltrated with VECTASHIELD PLUS Antifade Mounting Medium (Vector Laboratories, cat. H-1900-2).
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bioRxiv - Neuroscience 2024Quote: ... The slices were mounted on glass slides with a mounting solution containing DAPI (4’,6-diamidino-2-phenylindole; Vectashield with DAPI, Vector Laboratories, Burlingame, CA, USA). Images were acquired using a slide scanning system (Panoramic Scan ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were then incubated overnight with agitation at 4°C with Wisteria floribunda lectin (WFA, WFL, Biotinylated; Vector Laboratories, Newark, CA, B-1355-2) diluted in staining buffer (2% PBST and 2% NGS ...
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bioRxiv - Cancer Biology 2021Quote: ... Sections were transferred to citric acid-based antigen unmasking solution (Vector Laboratories) and heated in a 2100 antigen-retriever (Aptum Biologics) ...
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bioRxiv - Cancer Biology 2021Quote: ... After a 5 min wash with PBS the slide was incubated for 30 min with the Vectastain Elite ABC reagent followed by a 2-10 min incubation with a DAB peroxidase substrate solution (Vector Laboratories, Cat. No. SK-400) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... and then mounted with Vectashield® mounting media with 4’,6-diamidino-2-phenylindole (DAPI; H-1000 and H-1200; Vector laboratories, Inc, Burlingame, USA). Potential unspecific binding was evaluated by omission of the primary antibody in control sections that were otherwise processed similarly.
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bioRxiv - Developmental Biology 2024Quote: ... immunofluorescence was performed on paraffin sections using antibodies in Supplementary Table 2 and the following reagents: ImmPRESS® HRP Horse Anti-Rabbit IgG Polymer Detection Kit (Vector Labs, MP-7401-50). Following the application of TSA fluorophores (listed in Supplementary Table 2 ...
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Analysis of spike protein variants evolved in a novel mouse model of persistent SARS-CoV-2 infectionbioRxiv - Microbiology 2023Quote: ... The slides stained with rabbit anti-SARS-CoV-2 S polyclonal antibody or rabbit anti-ACE2 antibody were further incubated with biotinylated horse anti-rabbit antibody (Vector Laboratories, cat. no. BP 1100). All slides were then incubated with HRP-conjugated streptavidin (Vector Laboratories ...
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bioRxiv - Cell Biology 2022Quote: ... and incubated with a rabbit polyclonal anti-syncollin antibody for 2 h at room temperature followed by an FITC-conjugated goat anti-rabbit secondary antibody and 4′,6-diamidino-2-phenylindole (DAPI, Vector Laboratories, Burlingame, CA; 1.5 μg.mL−1).
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bioRxiv - Genetics 2022Quote: ... and either directly seeded on 10cm cell culture dishes or further enriched with Lotus Tetragonolobus Lectin (LTL; Vector labs biotynilated beads, cat. no. B-1325-2) LTL or Dolichos Biflorus Agglutinin (DBA ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated overnight at 4°C in slow agitation with primary antibody and/or biotinylated Wisteria Floribunda lectin (WFA, 1:1000, Vector Labs, USA, Ref. B-1355-2). The following primary antibodies were used ...
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bioRxiv - Immunology 2023Quote: ... antigen retrieval was performed using citric acid–based antigen unmasking solution (Vector Laboratories) on 5 μm sections from FFPE Swiss rolls of distal small intestine ...
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bioRxiv - Physiology 2024Quote: ... Slides were boiled in citric acid-based antigen retrieval solution (H-3300, Vector Labs) for 20 minutes.
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bioRxiv - Neuroscience 2024Quote: ... sections were rinsed with 0.1 M PB (4 x 5 min) and incubated in biotinylated goat anti-mouse IgG antibody (Vector Laboratories: 1/200 in 2% NGS in 0.1M PB) for 30 min ...
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bioRxiv - Molecular Biology 2023Quote: ... Microwave antigen retrieval in citric acid-based antigen unmasking solution (Vector Laboratories, Burlingame, CA, USA) was then performed ...
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bioRxiv - Synthetic Biology 2024Quote: ... heat-induced epitope retrieval was performed using citric acid-based antigen unmasking solution (Vector Laboratories) at 110°C for 15 minutes in a programmable antigen retrieval chamber (ARC ...
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bioRxiv - Cancer Biology 2021Quote: ... antigen unmasking was performed on rehydrated tissue sections using either a citric-acid retrieval buffer (Vector Labs) or Tris-EDTA retrieval buffer (10 mM Tris ...
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bioRxiv - Microbiology 2024Quote: ... Antigen retrieval in pre-heated citric acid-based buffer (1:100 in water, Vector Laboratories, Cat#H-3300) was performed in a pressure cooker (Instant Pot ...
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bioRxiv - Immunology 2021Quote: ... Tissues were then subjected to antigen retrieval in either Tris- or citric acid-based antigen unmasking solutions (Vector Laboratories), blocked in 10% normal goat serum ...
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bioRxiv - Cell Biology 2021Quote: ... the slides were washed in cold 3:1 ethanol:acetic acid and mounted in Vectashield medium with DAPI (Vector Laboratories).
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bioRxiv - Developmental Biology 2022Quote: ... 5 μm sections were de-paraffinized and treated with citric acid-based Antigen Unmasking Solution (Vector Laboratories, #H-3300) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... and antigenic sites were unmasked using either Tris-EDTA (10mM Tris-HCl, pH 9.0; 1mM EDTA; 0.05% Tween-20) or citric acid-based unmasking solution (Vector Laboratories) in Pascal pressure chamber (Dako) ...
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bioRxiv - Cell Biology 2024Quote: ... and incubated in antigen retrieval (1:100, antigen unmasking solution, citric acid based, catalog number: H-3300, Vector Laboratories) solution at 92°C for 10 mins ...
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bioRxiv - Microbiology 2020Quote: ... Remaining −2,3-linked sialic acid was detected by adding 100uL biotin-linked Mal II (2.5ug/mL; Vector Laboratories, B-1265) and incubating for 1h at RT ...
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bioRxiv - Microbiology 2022Quote: ... Antigen retrieval was performed prior to immunofluorescent staining by heating the tissue sections in citric acid buffer (pH 6.0; Vector Laboratories) at 95°C for 20 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... (recognizing Aβ and C99 but not holo-APP) or for 30 min in citric acid solution pH=6 at 90-100°C (Pressure cooker, Vector Laboratories) for 82E1 and anti-Iba1 (1/1,000 ...
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bioRxiv - Developmental Biology 2024Quote: Slides were kept at room temperature for 1hour followed by antigenic retrieval for 10 minutes at 90 degrees in citric acid solution (H3300, Vector Labs) following manufacturer instructions ...
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bioRxiv - Neuroscience 2023Quote: ... slides were deparaffinized and boiled for 20 min in citrate buffer (0.1M sodium citrate and 0.1M citric acid in distilled H2O) for antigen retrieval and incubated with 5% normal rabbit serum (Vector laboratory #S-5000) in PBS containing 0.2% Triton for 1 h at RT to block unspecific binding ...
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bioRxiv - Microbiology 2022Quote: ... Removal of α(2,3)-linked and α(2,6)-linked sialic acids on the cell surface was verified by staining with biotinylated Maackia amurensis lectin (MAL) I (5 μg/ml; Vector Laboratories; B-1265-1) or fluorescein-labeled Sambucus nigra lectin (SNA ...
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bioRxiv - Microbiology 2021Quote: ... was used to stain nucleic acids following washing away unbound secondary antibodies and samples were covered with VectaShield anti-fade reagent (Vector Laboratories, VWR, Cat# 101098-048) and a coverslip ...
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Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: Fluorescein-conjugated Sambucus nigra lectin (SNA) that binds sialic acid and Erythrina cristagalli lectin (ECL) that binds galactose were acquired from Vector Laboratories (SA-5001-1 and FL-1141-5, respectively).