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Citations for Vector Labs :
451 - 500 of 554 citations for 2 CHLOROMETHYL 6 METHOXY 1H BENZO D IMIDAZOLE since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... 4,6-diamidino-2-phenylindole (DAPI) was added to the embedding medium (4:1 Citifluor AF1 (Citifluor) and Vectashield (Vector Laboratories)) ...
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bioRxiv - Genetics 2021Quote: ... the sections were washed 3 times in PBS for 5 minutes each and mounted with Vectashield mounting medium containing 4,6-diamidino-2-phenylindole (DAPI, Vector Laboratories) and sealed with nail polish ...
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bioRxiv - Neuroscience 2024Quote: ... washed and incubated in horse anti-mouse biotinylated secondary (1:200 in 2% BSA and 0.1% Triton {TBS]; Vector Laboratories) for 180 min prior to washing and development in Streptavidin-tertiary reagent (1:500 in 2% BSA and 0.1% Triton [TBS] ...
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bioRxiv - Neuroscience 2024Quote: ... incubated in secondary antibody in 5% NGS for at least 2 overnights and mounted using Vectashield (Vector Laboratories H-1000). After each step ...
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bioRxiv - Neuroscience 2022Quote: ... and counterstained with 1 μg/mL DAPI (Thermo) for 2 minutes to label nuclei and mounted with VectaShield (Vector Laboratories). Images were taken using a confocal microscope (Zeiss LSM 800) ...
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bioRxiv - Molecular Biology 2023Quote: ... Double blocking was achieved with (1) H2O2 for 10 mins and (2) 2.5% normal horse serum for 1 hour (S-2012, Vector Labs). vWAT sections were incubated overnight at 4°C with primary antibody (CD68 ...
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bioRxiv - Neuroscience 2023Quote: ... for 2 h at RT followed by incubation in avidin-horseradish peroxidase complex (Vectastain ABC Elite, PK-6000; Vector Laboratories) for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... Tissue was then exposed to diaminobenzidine for 2 minutes using the DAB Peroxidase HRP Substrate Kit (Vector Laboratories, SK-4100) then washed with 1X PBS ...
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bioRxiv - Neuroscience 2024Quote: ... full-thickness colon segments of ∼1cm in length were blocked for 2 hrs in 1.5ml tubes containing 1 drop of Mouse-on-Mouse blocking reagent (MOM; Vector Labs) in 1ml of PBS with 0.5% Triton-X 100 ...
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bioRxiv - Neuroscience 2024Quote: ... Primary antibody was detected using a biotinylated secondary antibody (Table 2) by avidin-biotin peroxidase method (ABC Kit, Vector Laboratories) using diaminobenzidine (DAB ...
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Co-release of GABA and ACh from medial olivocochlear neurons fine tunes cochlear efferent inhibitionbioRxiv - Physiology 2024Quote: ... a second blocking for ∼2 h at room temperature with a Mouse blocker Reagent (2-3 drops in 2.5 ml PBS, MKB-2213-1, Vector Laboratories) was used ...
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bioRxiv - Biochemistry 2024Quote: ... the samples were washed four times with 1× PBS and mounted in Vectashield Vibrance antifade mounting media with 40,6-diamidino-2-phenylindole (DAPI; catalog no.: H-1800; Vector Laboratories). Images were acquired with a Zeiss LSM 880 confocal microscope with Airyscan ...
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bioRxiv - Cell Biology 2024Quote: ... Nuclei were stained using 49,6-diamidino-2-phenylindole (DAPI) and were subsequently overlaid with Vectashield (Vectashield; Vector Laboratories, Burlingame, CA) mounting medium for imaging with a Zeiss 900 confocal microscope with a 40X objective.
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bioRxiv - Molecular Biology 2021Quote: ... Slides were finally mounted with Vectashield Mounting Media with 4,6-diamidino-2-phenylindole as nuclear counterstain (Vector Laboratories, Cat# H-1200). Stainings were digitalized employing the Olympus BX61VS microscope and scanner software VS-ASW-S6 (Olympus ...
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bioRxiv - Genomics 2021Quote: ... Flies were dissected 10 to 40 days pi and organs viewed by fluorescence microscopy and imaged live or fixed in 2% paraformaldehyde and stained with DAPI in VECTASHIELD mounting medium (Vector Laboratories).
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bioRxiv - Neuroscience 2020Quote: ... Following 3 washes in PBS-T they were incubated for 2 hr at RT with the appropriate biotinylated secondary antibody (Vector Laboratories) diluted 1:500 in PBS-T ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were postfixed with 10 % NBF for 2-3 min before mounting on a clean glass slide using Vectashield antifade mounting media (Vector Laboratories). Image acquisition was performed on the LSM 700 laser confocal microscope (Zeiss) ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were washed and transferred to donkey anti-rabbit secondary antibody (1:500; Jackson Immuno) for 2 hr before detection with ABC (Vectastain ABC Kit, Vector Laboratories) and visualization with DAB substrate (Vector Laboratories) ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were then incubated overnight at 4°C with their respective primary antibodies (1%–2% BSA in TBST) followed by horseradish peroxidase IgG (Vector Laboratories)-conjugated secondary antibody (anti-rabbit and anti-mouse ...
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bioRxiv - Neuroscience 2022Quote: ... incubated in appropriate secondary antibody solutions for 2 hours (ChAT (1:200): rabbit anti-goat IgG (biotinylated; Vector Laboratories, BA-5000); PV (1:200) ...
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bioRxiv - Neuroscience 2021Quote: ... We then rinsed the sections in PBS and incubated them for 2 h with biotinylated anti‐rabbit IgG secondary antibody (BA‐1000, Vector Laboratories) diluted 1:600 in 4% BSA in 0.4% PBS‐TX ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated with primary antibody for 16-24 hrs at 4°C and then for 2 hrs at 4°C with biotinylated goat anti-rabbit IgG secondary antibody (1:800, Vector Laboratories) in 5% normal goat serum in 0.1% Triton-X-100 in TBS ...
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bioRxiv - Neuroscience 2020Quote: ... After the transfer the blots were blocked for 2 h in 1 x Animal-Free Blocker (SP-5030 Vector Labs, USA). The blots were incubated over night at 4°C under gentle agitation in the primary antibody solutions (1:300 goat anti-UCP1 ...
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bioRxiv - Developmental Biology 2021Quote: ... For IBA1 staining sections were subsequently incubated with a biotinylated F(ab’)2-Goat anti-Rabbit IgG (Vector Labs, CA, USA). All sections were then incubated with horseradish peroxidase (HRP)-conjugated streptavidin (Dako Agilent Pathology Solutions ...
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bioRxiv - Cell Biology 2020Quote: ... Muscle tissues were then placed into a microcentrifuge tube containing PBT with 2 % final concentration of normal goat serum (NGS; Vector Labs) for 30 mins ...
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bioRxiv - Neuroscience 2020Quote: ... two times for 2 min each before reacting with diaminobenzidine in the presence of nickel (DAB Peroxidase Substrate Kit, Vector Laboratories) according to the manufacturer instructions ...
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bioRxiv - Neuroscience 2022Quote: ... a thin slice of dorsal spinal cord (∼2 mm thick) with attached DR stumps was cut with a microscissors and mounted on slides in Vectashield (Vector Laboratories).
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bioRxiv - Bioengineering 2022Quote: ... incubation was for 2 hours at RT and secondary antibody (1:2000 in 1% BSA, Biotinylated horse anti-mouse, Vector Labs) incubation was for 1 hour at RT ...
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bioRxiv - Genomics 2022Quote: ... sections were incubated with Alexa dye–conjugated secondary antibodies diluted in blocking serum for 2 hours before being washed again and mounted with Vectashield Antifade Mounting Medium with DAPI (Vector Laboratories). Alexa Fluor® 488 Donkey IgG ...
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bioRxiv - Neuroscience 2022Quote: ... primary antibody overnight at 4 °C and revealed with a biotinylated goat anti-rabbit IgG secondary antibody (1:1,000, 2 hat RT, Vector Laboratories, VECTBA1000), the VECTASTAIN ABC Elite HRP kit (Vector Laboratories ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed with PBS-T and then incubated for 2 h at RT with biotinylated secondary antibody (1:200, Vector Laboratories). After subsequent washes with PBS-T ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Expression of MUC protein by Caco-2 cells was studied using biotinylated wheat germ agglutinin (WGA) (Vector Labs; Burlingame, CA, USA). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: ... This was followed by incubation with HRP conjugated 2° antibodies (α-mouse (PI-2000) and α-rabbit (PI-1000, Vector Labs)) in 5% Milk in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... Free-floating sections were washed (three times in 1xPBS for 10 min) and blocked for 2 hours with 10% normal goat serum (NGS, Vector Laboratories), 0.2% BSA (Carl Roth ...
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bioRxiv - Neuroscience 2023Quote: ... and hybridized with 2 µg/ml digoxigenin (DIG)- and dinitrophenol (DNP)-labeled probes in a hybridization buffer containing the Biotin solution (Vector Laboratories) overnight at 60°C in a humidified chamber ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were dehydrated by incubating for 2 min in increasing concentrations of EtOH (70%, 95%, and 100%) before mounting with Vectashield with DAPI (Vector laboratories). Images of at least 150 nuclei were acquired at 20X using a Zeiss Imager.M2 epifluorescence microscope equipped with a Axiocam 503 mono camera ...
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bioRxiv - Neuroscience 2023Quote: ... The slides were washed in 1x PBS three times for 2 minutes and covered with Vectashield mounting medium without DAPI (Vector Labs). Five imaging fields in the gray matter of the same case were captured using a fluorescent microscope (Keyence BZ-X710) ...
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bioRxiv - Neuroscience 2024Quote: ... was followed by incubation with the primary antibody (Table S2) at 4°C for 24 or 48h in 2% (vol/vol) serum and with the corresponding biotinylated antibody (Vector Laboratories) or alkaline-phosphatase antibody (ab6722 ...
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bioRxiv - Microbiology 2024Quote: ... The membrane proteins were separated by centrifugation at 104,000 g for 1 h at 4 °C and loaded onto a column prefilled with either 2 mL of agarose-bound WFA (Vector Laboratories) for S ...
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bioRxiv - Genomics 2024Quote: ... cells were stained with DAPI 2 μg per ml and mounted into slides using Vectashield (Vector Laboratories Cat # H-5700-60). Images were obtained with a Leica TCS-SL confocal microscope ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were then incubated for 2 hours at room temperature with horseradish-peroxidase-labelled secondary antibody (Goat Anti-Rabbit IgG Antibody; Vector Laboratories) diluted 1:10,000 in 5% dried non-fat milk powder in TBS-T ...
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bioRxiv - Neuroscience 2024Quote: Sections were then incubated in secondary antibody (biotinylated anti-goat IgG made in horse, 1:500, Vector Laboratories, see Table 2) for 1 hr in Tris B ...
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bioRxiv - Biophysics 2024Quote: ... Pel in the matrix of PA14 wild-type and ΔwspF was stained using GFP fluorescently labelled Wisteria floribunda lectin (WFL, FL-1351-2, Vector Laboratories). WFL is diluted in phosphate-buffered saline solution at 10 µ/ml concentration and loaded into a syringe ...
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bioRxiv - Cell Biology 2024Quote: ... Histopathology for three biotinylated mucin O-linked specific lectins: Griffonia (Bandeiraea) Simplidifolia Lectin I (GSL I, Vector Labs, B-1105-2); Maackia Amurensis Lectin II (MAL II ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated for 2 hours at room temperature with avidin–biotin–peroxidase (ABC; 1:250 in PB; VECTASTAIN Elite ABC kit, Vector Laboratories, PK6100). The immunoreaction was visualized with 3,3-diaminobenzidine (DAB) ...
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bioRxiv - Neuroscience 2022Quote: ... then mounted on slides in hard set mounting medium containing 4,6-diamidino-2-phenylindole (DAPI) (Vectashield, H-1500, Vector Laboratories, Burlingame, CA). A confocal system (TCS SP8 ...
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bioRxiv - Neuroscience 2022Quote: ... free–floating brain sections were rinsed in 0.1 M PBS (2 × 15 min) followed by a 30 min blocking in 3 % normal horse serum (Vector Laboratories, Burlingame, CA) and 0.3 % Triton X-100 in 0.1 M PBS ...
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bioRxiv - Developmental Biology 2020Quote: ... A small aliquot of embryo pellet (2 μL) was taken right before freezing and mixed with 10 μL VECTASHIELD® Antifade Mounting Medium with DAPI (Vector laboratories) and immediately frozen in dry ice ...
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bioRxiv - Microbiology 2021Quote: ... incubated with avidin-biotin complex kit (ThermoFischer Scientific, 32020) for 2 hrs and visualized with a 3,3’-diaminobenzidine kit (Vectastain, Vector Labs, SK-4100). Sections were then counterstained with cresyl violet for Nissl labeling.
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bioRxiv - Microbiology 2024Quote: ... The parasitophorous vacuoles (PV) of the parasites of were then stained with 2 μg/mL fluorescein labelled Vicia Villosa lectin (VVL; Vector Laboratories #ZG0124) in 1% BSA/PBS for 1 hour at room temperature then nuclei stained with 2 μg/mL DAPI (Merk #096M-4014V ...