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Citations for Mabtech :
201 - 250 of 292 citations for Streptavidin ZAP Antibody Kit mouse since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... and 5 μg/mL anti-perforin capture antibody mix (capture αPrf; Mabtech, combination of clones Pf-80/164). The resulting surfaces were micropatterned as described before with 10 μg/mL αLFA-1 + 10 μg/mL αCD16 + 5 μg/mL capture αPrf + 10 μg/mL BSA-AF555 ...
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bioRxiv - Immunology 2023Quote: ... washed and coated overnight with anti-IFN-ψ coating antibody (mAb-1-D1K, Mabtech, Cat. 3420-3-1000). Plates were washed and T cells were co-cultured with autologous LCL in RPMI/ 10% FCS/ 1% Pen/Strep at an effector ...
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bioRxiv - Immunology 2022Quote: Single cell suspensions from lungs and spleens of mice were stimulated with mouse IL-2 (R & D Systems, Cat # 402-ML; 0.5 μg/mL) and RD848 (Mabtech, Cat # 3611-5X; 1 μg/mL) for 3 days to induce nonspecific polyclonal expansion ...
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bioRxiv - Immunology 2020Quote: ... The detection antibody solution composed of PBS + 5 μg/mL anti-perforin conjugated with biotin (clone Pf-344, Mabtech) was added to the slide chambers ...
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bioRxiv - Immunology 2022Quote: ... and incubated for 2 h with an IFN-γ detection antibody (1 μg/ml; clone mAb-7B6-1; Mabtech) followed by 1 h incubation with AP-conjugated streptavidin (1:1000 in PBS ...
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bioRxiv - Immunology 2022Quote: ... Cell suspensions were transferred to pre-coated Human IFN-γ ELISpot plus kit (Mabtech) and developed after 20h according to manufacturer instructions ...
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bioRxiv - Microbiology 2019Quote: ... IFN-γ and IL-17A ELISPOT assays were performed using Human ELISpotplus kit (MabTech) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... and 1-2×105 cells were plated per well of a 96-well Millipore MAIPSWU plate coated with anti-IFNγ antibody according to the manufacturer’s instructions (3420-2HW-Plus, Mabtech Inc.). Peptide pools were added to the cells at a final concentration of 1μg/mL/peptide prior to incubation at 37°C overnight ...
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bioRxiv - Immunology 2020Quote: ... Cells were then discarded and plates were further incubated with biotinylated IFNγ antibody (Mab 7-B6-1; Mabtech, Stockholm, Sweden) and incubated for 2hr at 37 C° ...
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bioRxiv - Bioengineering 2022Quote: ... IL6 was captured to a level of 1300-2800 RU on an anti-IL6 monoclonal antibody (mAb clone 13A5, MABTECH) coupled to the chip surface and the interaction analysis of 4 selected Nbs was performed similarly as described above ...
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bioRxiv - Microbiology 2022Quote: ... and the plate was incubated for 2 h at room temperature with R4-6A2 biotinylated anti-IFN-γ antibody (Mabtech) at the concentration of 100 μg/mL ...
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bioRxiv - Immunology 2022Quote: ... Cells were removed and plates were then washed with PBS-0,05% Tween-20 prior incubation with biotinylated anti-IFN-γ antibody (7-B6-1, Mabtech) (2h ...
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bioRxiv - Immunology 2020Quote: ... cells were removed by washing (using PBS and PBS-Tween 0.02% solution) and 100 µl of biotinylated secondary anti-IFN-γ antibody (clone 7-B6-1, Mabtech) in blocking buffer (PBS 0.5% BSA ...
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bioRxiv - Immunology 2020Quote: ... ELISpot plates were coated with IFN-γ capture antibody (2 μg/ml per well, Mabtech mAb 1-D1K, 3420-7), in D-PBS and then blocked with RPMI-1640 supplemented media ...
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bioRxiv - Immunology 2021Quote: Antigen-specific cellular responses were measured by IFNγ Fluorospot assay with all antibodies and reagents from Mabtech (Nacka Strand, Sweden). Plates were coated overnight at 4°C with a mouse anti-human IFNγ (clone 1-D1K ...
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bioRxiv - Immunology 2020Quote: ... the plates were developed with human biotinylated IFN-γ detection antibody (Clone 7-B6-1, 1 μg/ml, Mabtech, Sweden), followed by incubation with Streptavidin-AP (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... Fluorescent spots indicating a single mASC and relative volume of antibody produced were detected with an IRIS FluoroSpot Reader and counted with Apex software (Mabtech). Positivity thresholds were set for both S1 and N mASCs based on prepandemic control samples’ average S1 or N plus three standard deviations.
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bioRxiv - Immunology 2023Quote: ... and the plate was incubated for 2 h at room temperature with R4-6A2 biotinylated anti-IFN-γ antibody (Mabtech) at the concentration of 100 μg/mL ...
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Metabolomics reveals nucleoside analogs for regulating mucosal-associated invariant T cell responsesbioRxiv - Immunology 2023Quote: ... The IFNγ+ MAIT cell spots were then developed with an indirect immunostain approach sequentially using a biotinylated anti-human IFNγ antibody (Mabtech), ExtraAvidin conjugated by alkaline phosphatase (Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... cells were cultured for 24 h and detected using a commercial kit (MABTECH, Nacka, Sweden) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: Immunoglobulins (Ig) in culture supernatants were measured using a human IgG ELISA development kit (Mabtech) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... IFN-γ and IL-2-secreting cells were detected using ELISpot kits (DAKEWE and MABTECH) according to the manufacture protocol and spots were counted using an ELISpot Analyzer AT-SPOT(SINSAGE).
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bioRxiv - Immunology 2024Quote: ... IFNγ and TNFα ELISpot were performed using monkey cytokine specific ELISpotPLUS (ALP) KITs (Mabtech, Inc.) following the manufacturer’s instructions ...
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Dendritic cells focus CTL responses toward highly conserved and topologically important HIV epitopesbioRxiv - Immunology 2020Quote: ... T cells were resuspended at a concentration of 3×105 cells per milliliter and 100 μl (3×104 cells/well) were transferred to IFNγ-antibody coated 96-well ELISpot plates (5μg/ml anti-IFNγ mAb 1-D1K, Mabtech, Stockholm, Sweden). Individual 9mer peptide dilutions were done in a separate 1 ml deep-well 96-well plate at a final concentration of 2μg/ml and 100 μl of this dilution was added to T cell-containing wells to give a final peptide concentration of 1μg/ml ...
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bioRxiv - Immunology 2019Quote: ... was coated overnight at 4 °C with 10 μg ml−1solution of anti-IFN-γ monoclonal antibody (Mabtech clone 1-D1K) in a buffer solution of 0.1 M Na2CO3 ...
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bioRxiv - Immunology 2022Quote: ... followed by 2 hours of incubation at 37°C with biotinylated anti-human IFN-γ monoclonal antibodies (clone 7-B6-1, Mabtech) diluted at 1μg/ml in PBS ...
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bioRxiv - Immunology 2023Quote: IFNγ and IL-17 production was measured by a Fluorospot assay with all antibodies and reagents from Mabtech (Nacka Strand, Sweden). Plates were coated overnight at 4°C with an antibody mixture containing mouse anti-human IFNγ (clone 1-D1K ...
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bioRxiv - Immunology 2021Quote: ... ELISpot assays were performed using the Rat IFN-γ ELISpotPLUS kit (MABTECH, Cat. No.: 3220- 4APW), Rat IL-4 T cell ELISpot kit (U-CyTech ...
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bioRxiv - Immunology 2020Quote: An ELISPOT assay was performed with the Monkey IFN-γ ELISPOT Kit (Mabtech, Cincinnati, OH, USA) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: IFN-γ ELISpot assays were performed using the Human IFN-γ ELISpot PLUS ALP kit (Mabtech) according to the manufacturer’s protocol with some modifications ...
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bioRxiv - Bioengineering 2023Quote: ... Anti-IFNγ and anti-IL-4 ELISpot PLUS kits were purchased from Mabtech (Nacka Strand, Sweden). PepTivator Ovalbumin epitope mix was provided by Miltenyi Biotec (Nordrhein-Westfalen ...
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bioRxiv - Immunology 2024Quote: ... Frequencies of IFN-γ or IL-2-secreting T cells were assessed using commercial kits (Mabtech) according to the manuals ...
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bioRxiv - Immunology 2021Quote: ... cells were discarded and FluoroSpot plates were washed and further incubated for 2 hours with cytokine antibodies (mAbs 7-B6-1-BAM; Mabtech, Stockholm, Sweden). Subsequently ...
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bioRxiv - Immunology 2020Quote: ... cells were discarded and FluoroSpot plates were washed and further incubated for 2hr with cytokine antibodies (mAbs 7-B6-1-BAM, 5A10 biotinylated, MT20D9-WASP, respectively; Mabtech, Stockholm, Sweden). Subsequently ...
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bioRxiv - Immunology 2020Quote: ... Controls included 1×105 effector CD8+ T cells co-cultured with anti-human CD3 monoclonal antibody (0.5 µg/mL, Mabtech, 3605-1-1000), or with media alone ...
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bioRxiv - Immunology 2020Quote: ... The plate was washed and IFN-I assayed using a mixture of biotinylated detection antibodies against IFN-α (Mabtech, MT2/4/6). Avidin-conjugated alkaline phosphatase (Sigma ...
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bioRxiv - Immunology 2021Quote: 96-well FluoroSpot plates were coated with anti-cytokine antibodies for IFNγ and IL-5 (mAbs 1-D1K and TRFK5, respectively; Mabtech, Stockholm, Sweden) at a concentration of 10μg/mL ...
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bioRxiv - Immunology 2021Quote: ... cells were discarded and plates were washed before the addition of cytokine antibodies (mAbs 7-B6-1-BAM and 5A10-WASP; Mabtech, Stockholm, Sweden). After a 2-hour incubation ...
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bioRxiv - Immunology 2019Quote: IL-8 was quantified in plasma by Human IL-8 (CXCL8) ELISA development kit (3560-1H, Mabtech) according to manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: Rhesus macaque PBMCs were tested with commercially available NHP IFNγ and IL-4 ELISpot assay kits (Mabtech). Cryopreserved rhesus macaque PBMCs were thawed in pre-warmed AIM-V media (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: T cell responses were assessed by ELISPOT assay using a Human IFNγ ELISPOTPro kit (Mabtech, NS, Sweden) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ELISpot assays were performed using precoated monkey IFN-γ ELISpotPLUS Kits according to the manufacturer’s instructions (Mabtech). Briefly ...
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bioRxiv - Immunology 2022Quote: A fluorospot 96-well plate from a Human INF-γ/IL-2 FluorospotPLUS kit (Mabtech AB, Sweden) was washed x3 with PBS and then blocked with optimizer media-10% FCS according to manufacturer description at room temperature until use ...
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bioRxiv - Immunology 2023Quote: ... ELISpot assay was performed on treated PBMCs using ELISpot Pro: Human IFN-γ (ALP) kit (Mabtech, Sweden), following manufacture’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... Plates were developed by washing 6 times in PBS/0.05%Tween 20 followed by incubation with biotinylated IFN-γ detection antibody (0.5 mg/mL; Mabtech; 2 hours, 37°C). This was followed by a further 6 washes in PBS/0.05%Tween 20 and incubation with avidin DH-coupled biotinylated peroxidase H complex (Vectastain Elite ABC Kit ...
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bioRxiv - Immunology 2020Quote: Rhesus macaque PBMCs were tested with commercially available NHP IFNγ and IL-4 ELISpot assay kits (Mabtech, Sweden). Cryopreserved rhesus macaque PBMCs were thawed in pre-warmed AIM-V media (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... IFN-γ secreting cells were detected by Human IFN-γ ELISpot Basic Kit (ALP) (Mabtech AB, 3420-2A). The plates were developed using the AP Conjugate Substrate Kit (Biorad ...
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bioRxiv - Immunology 2021Quote: ... Cell culture supernatants were analysed with the bovine IFNγ ELISA development kit (MABTECH, Cat no. 3119-1H-20) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Cell suspensions were transferred to pre-coated Human IFN-γ ELISpot plus kit plates (Mabtech, Nacka Strand, Sweden) and developed after 20h per manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... FluoroSpot assays were performed according to the manufacturer’s recommendations (FluoroSpot Plus: Human IFNγ/IL-2/TNFα kit, MABTech). Briefly ...