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Citations for Mabtech :
51 - 68 of 68 citations for SARS CoV 2 Spike N Terminal Domain NTD Sheep Fc Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2022Quote: ... The cells were incubated for 20 hours at 37°C in 5% CO2 incubator in pre-coated Ferret IFNγ-ELISpot plates (3112-4APW-2, Mabtech). The assay was performed following the manufacturers protocol ...
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bioRxiv - Immunology 2022Quote: ... PBMC were thawed and cultured at 37°C and 5% CO2 in the presence of 1 μg/mL R848 and 10 ng/mL of recombinant interleukin (IL) 2 (both from Mabtech).
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bioRxiv - Immunology 2020Quote: ... ELISpot plates were coated with IFN-γ capture antibody (2 μg/ml per well, Mabtech mAb 1-D1K, 3420-7), in D-PBS and then blocked with RPMI-1640 supplemented media ...
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bioRxiv - Immunology 2023Quote: ... and the plate was incubated for 2 h at room temperature with R4-6A2 biotinylated anti-IFN-γ antibody (Mabtech) at the concentration of 100 μg/mL ...
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bioRxiv - Immunology 2023Quote: ... the 2CZ T cell hybridoma was used and the IL-2 secreted upon its activation was quantified using an ELISPOT assay (Mabtech) as previously described 7.
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bioRxiv - Immunology 2022Quote: The T cell immune responses in rhesus macaques were detected using the PBMCs with commercially available NHP IFN-γ and IL-2 ELISpot assay kits (Mabtech), and NHP IL-4 ELISpot assay kit (U-CyTech) ...
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bioRxiv - Immunology 2020Quote: ... and incubated for 2 hr at room temperature with biotinylated anti-IFN-γ (clone mAb-7B6-1; 1 μg/ml Mabtech). After six further washes ...
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bioRxiv - Microbiology 2021Quote: ... at an E:T ratio of 5:1 for 24 hours followed by measurement of specific cytokine production using the human IL-2 ELISpot kit (Mabtech AB) and ELIPHOTO Counter (Minerva Tech).
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bioRxiv - Immunology 2021Quote: ... followed by two washes with PBS and then incubated for 2 hr at RT with biotinylated anti-IFN-γ (1 μg/mL; clone mAb-7B6-1; Mabtech). After six further washes ...
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bioRxiv - Immunology 2022Quote: ... followed by 2 hours of incubation at 37°C with biotinylated anti-human IFN-γ monoclonal antibodies (clone 7-B6-1, Mabtech) diluted at 1μg/ml in PBS ...
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bioRxiv - Immunology 2021Quote: ... splenocytes or lung leukocytes were stimulated with 1 μg/ml R848 and 10 ng/ml recombinant human IL-2 (Mabtech In, OH). Millipore ELISPOT plates (Millipore Ltd ...
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bioRxiv - Immunology 2021Quote: ... cells were discarded and FluoroSpot plates were washed and further incubated for 2 hours with cytokine antibodies (mAbs 7-B6-1-BAM; Mabtech, Stockholm, Sweden). Subsequently ...
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bioRxiv - Cancer Biology 2021Quote: ... Plates were developed by washing 6 times in PBS/0.05%Tween 20 followed by incubation with biotinylated IFN-γ detection antibody (0.5 mg/mL; Mabtech; 2 hours, 37°C). This was followed by a further 6 washes in PBS/0.05%Tween 20 and incubation with avidin DH-coupled biotinylated peroxidase H complex (Vectastain Elite ABC Kit ...
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bioRxiv - Immunology 2020Quote: ... Cellular immune responses were measured by IFN-γ FluoroSpot assay according to the manufacturer’s instructions (Cat. No. FSP-4246-2 Mabtech, Inc., Cincinnati, OH). Briefly ...
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bioRxiv - Immunology 2022Quote: ... kits with pre-coated plates for enumeration of cells secreting IFN-γ and IL-17A were purchased from Mabtech (Cat. No. FSP-414443-2). Spots were enumerated on an AID iSpot EliSpot/ FluoroSpot Reader.
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bioRxiv - Immunology 2021Quote: ... The remaining time-points were evaluated using Monkey IFNγ/IL-4 FluoroSpot FLEX kit and Monkey IL-2 FluoroSpot FLEX kit (Mabtech, X-21A16B and X-22B) following manufacturer instructions ...
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bioRxiv - Immunology 2022Quote: Single cell suspensions from lungs and spleens of mice were stimulated with mouse IL-2 (R & D Systems, Cat # 402-ML; 0.5 μg/mL) and RD848 (Mabtech, Cat # 3611-5X; 1 μg/mL) for 3 days to induce nonspecific polyclonal expansion ...
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bioRxiv - Immunology 2023Quote: ... Wells were washed and incubated for 2 hours with biotinylated anti-IFN-ψ detection antibody (mAb-7-B6-1-Biotin, Mabtech, Cat. 3420-6-250). Afterwards ...