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Citations for Mabtech :
151 - 177 of 177 citations for PD 1 Human HEK293 mFc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Cells were removed and plates were then washed with PBS-0,05% Tween-20 prior incubation with biotinylated anti-IFN-γ antibody (7-B6-1, Mabtech) (2h ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed 6X with PBS and incubated with 50 µl of streptavidin-alkaline phosphatase conjugate (1:1000 dilution; Mabtech) diluted in PBS 1% FBS ...
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bioRxiv - Cancer Biology 2021Quote: ... and the wells incubated with 100 μL of 1 μg/ml of the R4-6A2 biotinylated anti-IFN-γ (Mabtech) for 2 hours at r.t ...
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bioRxiv - Immunology 2022Quote: ... PBMC were thawed and cultured at 37°C and 5% CO2 in the presence of 1 μg/mL R848 and 10 ng/mL of recombinant interleukin (IL) 2 (both from Mabtech).
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bioRxiv - Immunology 2020Quote: ... cells were removed by washing (using PBS and PBS-Tween 0.02% solution) and 100 µl of biotinylated secondary anti-IFN-γ antibody (clone 7-B6-1, Mabtech) in blocking buffer (PBS 0.5% BSA ...
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bioRxiv - Immunology 2020Quote: ... ELISpot plates were coated with IFN-γ capture antibody (2 μg/ml per well, Mabtech mAb 1-D1K, 3420-7), in D-PBS and then blocked with RPMI-1640 supplemented media ...
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bioRxiv - Immunology 2024Quote: ... Plates were washed 6x times with PBST and plated with streptavidin-HRP conjugate (1:1000 dilution; catalogue #3310-9-1000, Mabtech) for 1 hour at room temperature in the dark ...
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Dendritic cells focus CTL responses toward highly conserved and topologically important HIV epitopesbioRxiv - Immunology 2020Quote: ... T cells were resuspended at a concentration of 3×105 cells per milliliter and 100 μl (3×104 cells/well) were transferred to IFNγ-antibody coated 96-well ELISpot plates (5μg/ml anti-IFNγ mAb 1-D1K, Mabtech, Stockholm, Sweden). Individual 9mer peptide dilutions were done in a separate 1 ml deep-well 96-well plate at a final concentration of 2μg/ml and 100 μl of this dilution was added to T cell-containing wells to give a final peptide concentration of 1μg/ml ...
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bioRxiv - Immunology 2019Quote: ... was coated overnight at 4 °C with 10 μg ml−1solution of anti-IFN-γ monoclonal antibody (Mabtech clone 1-D1K) in a buffer solution of 0.1 M Na2CO3 ...
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bioRxiv - Immunology 2020Quote: ... IFN-γ spot forming cells (SFC) were detected by staining membranes with anti-mouse IFN-γ biotin mAb (1 µg/mL; clone R46A2, Mabtech) followed by streptavidin-alkaline phosphatase (1 µg/mL ...
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bioRxiv - Immunology 2020Quote: ... and a commercial SARS-CoV-2 peptide pool (SARS-CoV-2 S N M O defined peptide pool (3622-1; Mabtech, Sweden) were included as assay controls ...
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bioRxiv - Microbiology 2023Quote: ... The plates were incubated at 37 °C in 5 % CO2 for 18 h and washed with PBS before the addition of 1 µg/ml biotinylated anti-IFN-γ mAb (Mabtech) at room temperature for 2 h ...
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bioRxiv - Immunology 2021Quote: ... cells were discarded and FluoroSpot plates were washed and further incubated for 2 hours with cytokine antibodies (mAbs 7-B6-1-BAM; Mabtech, Stockholm, Sweden). Subsequently ...
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bioRxiv - Immunology 2022Quote: ... ELISpot assays for IFNγ were performed as instructed by the manufacturer using the Mouse IFNγ ELISpot Set (MabTech, cat# Q00824-1×20210623DS). 0.1×106 mouse splenocytes were plated into each well and stimulated overnight with 0.2 µg per peptide per well of RBD-derived overlapping peptides (GenScript).
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bioRxiv - Immunology 2020Quote: ... cells were discarded and FluoroSpot plates were washed and further incubated for 2hr with cytokine antibodies (mAbs 7-B6-1-BAM, 5A10 biotinylated, MT20D9-WASP, respectively; Mabtech, Stockholm, Sweden). Subsequently ...
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bioRxiv - Immunology 2020Quote: ... for the enumeration of total IgG-secreting cells with 1 µg per well of anti-porcine IgG (mAb, MT421, Mabtech AB, Sweden) or with culture medium supplemented with 10% FBS (background) ...
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bioRxiv - Immunology 2021Quote: 96-well FluoroSpot plates were coated with anti-cytokine antibodies for IFNγ and IL-5 (mAbs 1-D1K and TRFK5, respectively; Mabtech, Stockholm, Sweden) at a concentration of 10μg/mL ...
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bioRxiv - Immunology 2021Quote: ... cells were discarded and plates were washed before the addition of cytokine antibodies (mAbs 7-B6-1-BAM and 5A10-WASP; Mabtech, Stockholm, Sweden). After a 2-hour incubation ...
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bioRxiv - Immunology 2020Quote: ... and for the enumeration of total IgG-secreting cells with 1 µg per well of anti-porcine IgG (mAb, MT421, Mabtech AB, Sweden) or with culture medium supplemented with 10% FBS (media background control) ...
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bioRxiv - Immunology 2022Quote: ... were pre-wet with 30% ethanol for 60 seconds and then coated with 100 µl primary anti-IFN-γ antibody solution (10 µg/ml of 1-D1K coating antibody from Mabtech, Cincinnati, OH) OVN at 4°C ...
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bioRxiv - Immunology 2024Quote: ... were pre-wet with 30% ethanol for 60 seconds and then coated with 100 µl primary anti-IFN-γ antibody solution (10 µg/ml of 1-D1K coating antibody from Mabtech, Cincinnati, OH) OVN at 4°C ...
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bioRxiv - Immunology 2020Quote: ... The following morning plates were washed with 1x PBS-T before being coated with 3 μg/mL streptavidin-ALP secondary antibody (Mabtech, clone 7-B6-1) and incubated for 2 hours at room temperature ...
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bioRxiv - Immunology 2020Quote: ... at 37 °C and 5% CO2 with overlapping peptide pools representative of either S1 or S2 SARS-CoV-2 proteins (JPT, #PM-WCPV-S) at 1 μg/ml each or DMSO in 96-well ELISpot IFN-γ plates (Mabtech #3321-4APW-2) in triplicates ...
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bioRxiv - Immunology 2021Quote: ... washed with sterile PBS and coated overnight at 4 °C with anti–IFN-γ antibody (10 µg/ml in PBS; clone 1-D1K; Mabtech, Nacka Strand, Sweden). Prior to use ...
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bioRxiv - Immunology 2022Quote: Single cell suspensions from lungs and spleens of mice were stimulated with mouse IL-2 (R & D Systems, Cat # 402-ML; 0.5 μg/mL) and RD848 (Mabtech, Cat # 3611-5X; 1 μg/mL) for 3 days to induce nonspecific polyclonal expansion ...
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bioRxiv - Immunology 2023Quote: ... Wells were washed and incubated for 2 hours with biotinylated anti-IFN-ψ detection antibody (mAb-7-B6-1-Biotin, Mabtech, Cat. 3420-6-250). Afterwards ...
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bioRxiv - Immunology 2020Quote: ... were pre-wet with 70% methanol and then coated with 100 µl primary anti-IFN-γ antibody solution (10 µg/ml of 1-D1K coating antibody from Mabtech in PBS, pH 7.4, V-E4) OVN at 4°C ...