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Citations for Mabtech :
101 - 150 of 255 citations for Oxytocin ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Plates contained additional no target cell negative control and anti-CD3 antibody (CD3-2, Mabtech) positive control wells ...
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bioRxiv - Immunology 2020Quote: ... and the plates were incubated with the detection antibody 7-B6-1-biotin (Mabtech, Sweden) diluted 1:1000 in PBS + 0.5% FCS and incubated for two hours at room temperature ...
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bioRxiv - Immunology 2020Quote: ... the plates were developed with human biotinylated IFN-γ detection antibody (7-B6-1, MabTech), followed by incubation with Streptavidin-AP (MabTech ...
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bioRxiv - Immunology 2020Quote: ... aliquots of PBMC (105/well) were seeded in duplicate wells of 96-well plates (MABTECH) pre-coated with the primary IFN-γ antibody and stimulated with mitogens PHA ...
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bioRxiv - Immunology 2020Quote: ... the plates were developed with human biotinylated IFN-γ detection antibody (7-B6-1, Mabtech), followed by incubation with Streptavidin-AP (Mabtech ...
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bioRxiv - Immunology 2023Quote: ... Developed spots on the ELISpot plate were then enumerated using an ELISpot reader (Mabtech, Sweden). The reactivity was determined by measuring the fold increase in the number of spots of PBMCs treated with mutant peptides relative to those treated with wild type peptides ...
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bioRxiv - Cancer Biology 2023Quote: ... Developed spots on the ELISpot plate were then counted using an ELISpot reader (Mabtech, Sweden). Reactivity was determined by measuring the fold increase in the number of spots in PBMCs treated with mutant peptides compared to those treated with wild-type peptides ...
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bioRxiv - Immunology 2020Quote: ... and IL-5 (mAbs 1-D1K, MT25C5, TRFK5, respectively; Mabtech, Stockholm, Sweden) at a concentration of 10μg/mL.
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bioRxiv - Microbiology 2022Quote: ... The air-dried plates were read using the automated ELISPOT reader (Mabtech IRIS FluoroSpot/ELISpot reader) for calculating spot-forming cells.
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bioRxiv - Immunology 2023Quote: ... Washed plates were incubated with alkaline-phosphatase-conjugated mAb for IFN-γ (Mabtech #7-B6-1) and with biotinylated mAb for IL-2 (Mabtech #MT8G10) ...
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bioRxiv - Immunology 2022Quote: ... were coated with human IFN-γ antibody (1-D1K, Mabtech; 5 μg/ml) overnight at 4°C ...
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bioRxiv - Genomics 2020Quote: ... Cells were stimulated with soluble anti-CD3 (5 μg/mL; MabTech, Cincinnati, OH) and anti-CD28 antibodies (10ug/mL ...
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bioRxiv - Immunology 2019Quote: ... 5-Bromo-4-chloro-3-indolyl phosphate/Nitro blue tetrazolium (BCIP/NBT, Mabtech) was used to develop the immune-spot according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... the plates were developed with human biotinylated IFN-γ detection antibody (7-B6-1, Mabtech; 1:2000), followed by incubation with Streptavidin-AP (Mabtech ...
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bioRxiv - Immunology 2020Quote: ... Plates were substrated for 8-12 minutes using 0.5µM filtered 3’3’5’5’-Tetramethylbenzidine (TMB substrate) (Mabtech, 3652-F10) and the plates were evaluated with an AID automated microplate ELISpot reader and associated software (AID ELISpot Version 7.0).
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bioRxiv - Immunology 2023Quote: ... 96-well nitrocellulose-bottom plates pre-coated with anti-mouse IFNγ monoclonal antibody were purchased from Mabtech. The plates were blocked with RPMI-10% FBS for at least 30 minutes ...
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bioRxiv - Immunology 2021Quote: ... 100,000 cells were plated in each well of the pre-coated Immobilon-FL PVDF 96 well plates (Mabtech), stimulated with the respective antigen at the respective concentration of 5 μg/ml and incubated at 37°C in a humidified CO2 incubator for 20-24 hrs ...
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bioRxiv - Immunology 2021Quote: ... and 2 x 105 splenocytes were plated into each well of the Mouse IFN-γ ELISpotPLUS plates (MabTech). The splenocytes were stimulated for 20 hours at 37°C with RBD peptides (15-mer peptides overlapping by 9 amino acid spanning the RBD of SARS-CoV-2 spike protein ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed with PBS and then incubated with anti-monkey or anti-mouse IFNγ mAb biotin (Mabtech) for two hours ...
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bioRxiv - Immunology 2021Quote: ... 2×105 cells were added to each well of pre-coated Immobilon-FL PVDF 96-well plates (Mabtech) in the presence of 2 μg/ml peptide pool ...
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bioRxiv - Immunology 2020Quote: ... Plates were then washed a further six times and developed for 20 min with BCIP/NBT Substrate (Mabtech). All assays were performed in duplicate ...
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bioRxiv - Immunology 2020Quote: ELISpot plates were coated with 10 μg/mL anti-human IFN-γ coating antibody (Mabtech, clone 1-D1K) and incubated overnight at 4C° ...
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bioRxiv - Immunology 2023Quote: ... 2×103 transfected T cells were plated per well in a pre-coated 96-well ELISpot plate (Mabtech) together with 5×104 PHA-activated autologous CD4 T cells as antigen-presenting cells and challenged with the specific peptide ...
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bioRxiv - Immunology 2019Quote: IFN-γ ELISPOT assay strip plate which was pre-coated with anti-human IFN-γ mAb (1-D1K, Mabtech) was washed with PBS and blocked with RPMI-1640 containing 10% FBS ...
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bioRxiv - Microbiology 2022Quote: ... Miltenyi Biotech) for 24 h in ELISpot plates coated with anti-mouse-IFN antibody (Mabtech AB, Nacka Strand, Sweden). The procedures were carried out according to the manufacturer’s instructions (Mabtech ...
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bioRxiv - Immunology 2023Quote: ... ELISpot assay plates were prepared and processed as per the manufacturer’s instructions (3321-4HPT-10, Mabtech Inc., Cincinnati, OH). ELISpots were enumerated by machine (CTL S6 Entry M2 ...
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bioRxiv - Immunology 2021Quote: ... a Human ELISpotBASIC IgG kit (Mabtech) was used ...
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bioRxiv - Immunology 2021Quote: ... and mIFN-γ Elispot kit (Mabtech) [23] ...
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bioRxiv - Molecular Biology 2021Quote: ... human Apolioprotein A1 ELISAPRO kits (Mabtech) were used to quantify apoA-I levels (as normalized to total protein ...
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bioRxiv - Immunology 2022Quote: ... Human Perforin ELISABASIC kit (HRP) (MabTech), Human Granzyme B ELISABASIC kit (HRP ...
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bioRxiv - Immunology 2023Quote: Murine and rat ELISpot kits (Mabtech) were used with 5×105 splenocytes/well in RPMI with L-glutamine ...
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bioRxiv - Immunology 2020Quote: ... were coated with 50 μl of anti-mouse IFN-γ mAb AN18 (5 µg/ ml; Mabtech) overnight at 4°C before being washed and blocked with cRMPI 10% FBS for 1 hr at 37C ...
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bioRxiv - Microbiology 2023Quote: ... that had been precoated with 5 µg/ml anti-IFN-γ monoclonal antibody (mAb) AN18 (Mabtech). The plates were incubated at 37 °C in 5 % CO2 for 18 h and washed with PBS before the addition of 1 µg/ml biotinylated anti-IFN-γ mAb (Mabtech ...
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bioRxiv - Immunology 2021Quote: ... Plates were then washed and incubated with biotinylated anti-mouse IFN-γ antibody (clone R4-6A2; Mabtech; 1 μg/mL) followed by streptavidin-alkaline phosphatase (Mabtech ...
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bioRxiv - Immunology 2019Quote: ... and 1-2×105 cells were plated per well of a 96-well Millipore MAIPSWU plate coated with anti-IFNγ antibody according to the manufacturer’s instructions (3420-2HW-Plus, Mabtech Inc.). Peptide pools were added to the cells at a final concentration of 1μg/mL/peptide prior to incubation at 37°C overnight ...
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bioRxiv - Immunology 2020Quote: ... Cells were then discarded and plates were further incubated with biotinylated IFNγ antibody (Mab 7-B6-1; Mabtech, Stockholm, Sweden) and incubated for 2hr at 37 C° ...
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bioRxiv - Microbiology 2022Quote: ... and the plate was incubated for 2 h at room temperature with R4-6A2 biotinylated anti-IFN-γ antibody (Mabtech) at the concentration of 100 μg/mL ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed 6X with PBS and incubated with 50 µl of streptavidin-alkaline phosphatase conjugate (1:1000 dilution; Mabtech) diluted in PBS 1% FBS ...
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bioRxiv - Immunology 2022Quote: ... Biotinylated antibody and Streptavidin-HRP were added successively after cell lysis.Then AEC Peroxidase Substrate were added and spots counted using an ELISpot plate reader (Mabtech IRIS™ Fluorospot Reader, Mabtech). Spot numbers were evaluated using Mabtech Apex™ software v.1.1.52.121.
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bioRxiv - Immunology 2020Quote: ... ELISpot plates were coated with IFN-γ capture antibody (2 μg/ml per well, Mabtech mAb 1-D1K, 3420-7), in D-PBS and then blocked with RPMI-1640 supplemented media ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed with PBS/0.05% Tween 20 and incubated with streptavidin – alkaline phosphatase conjugate (Strep-ALP, Mabtech AB, Sweden). After a final wash ...
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bioRxiv - Immunology 2020Quote: ... the plates were developed with human biotinylated IFN-γ detection antibody (Clone 7-B6-1, 1 μg/ml, Mabtech, Sweden), followed by incubation with Streptavidin-AP (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... Plates were then washed six times and developed using VECTASTAIN® Elite ABC-HRP according to the manufacturer’s instructions (Mabtech). All assays were performed in duplicate ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed with PBS/0.05% Tween 20 and incubated with streptavidin – alkaline phosphatase conjugate (Strep-ALP, Mabtech AB, Sweden). After a final wash ...
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bioRxiv - Immunology 2022Quote: ... Splenocytes were counted and 250,000 cells were plated per well into pre-covered plates and stimulated overnight with 10 μg/mL SARS-CoV-2 (S1) peptide pool (Mabtech) or medium as negative control or PHA 2 μg/mL as positive experimental control ...
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bioRxiv - Immunology 2022Quote: ... we washed off the cells and developed the plates using biotinylated R4-6A2 anti-IFN-γ (Mabtech #3321-6-250) and BVD6-24G2 anti-IL4 (Mabtech #3311-6-250 ...
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bioRxiv - Immunology 2023Quote: ... and the plate was incubated for 2 h at room temperature with R4-6A2 biotinylated anti-IFN-γ antibody (Mabtech) at the concentration of 100 μg/mL ...
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bioRxiv - Immunology 2022Quote: ... the ELISPOT plates were washed and stained with anti-IFN-γ-biotin followed by streptavidin-conjugated HRP (3420-2HW-Plus, Mabtech). Plates were developed using TMB substrate and read using a CTL-ImmunoSpot® S6 Analyzer (Cellular Technology Limited ...
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Dendritic cells focus CTL responses toward highly conserved and topologically important HIV epitopesbioRxiv - Immunology 2020Quote: ... T cells were resuspended at a concentration of 3×105 cells per milliliter and 100 μl (3×104 cells/well) were transferred to IFNγ-antibody coated 96-well ELISpot plates (5μg/ml anti-IFNγ mAb 1-D1K, Mabtech, Stockholm, Sweden). Individual 9mer peptide dilutions were done in a separate 1 ml deep-well 96-well plate at a final concentration of 2μg/ml and 100 μl of this dilution was added to T cell-containing wells to give a final peptide concentration of 1μg/ml ...
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bioRxiv - Cell Biology 2022Quote: For the ELIspot a commercial kit (Mabtech) was used ...