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Citations for Mabtech :
101 - 150 of 166 citations for Mouse IgG2a Isotype Control Antibody Biotin 15H6 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... The plates were washed and a biotinylated goat anti-human IgA antibody (Mabtech) with cross-reactivity to monkey antibodies was added to all wells ...
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bioRxiv - Immunology 2024Quote: ... ELISPOT plates were coated with 50 μl of anti–IFN-γ antibody (Mabtech, clone 1-D1K ...
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bioRxiv - Microbiology 2021Quote: ... Splenocytes were counted and 100,000 cells/well added to the plates of the Mouse IFN-g ELISpotPLUS (HRP) kit (MABTECH). Cells were then stimulated for 18 hours at 37 °C with SARS-CoV-2 S PepTivator peptides (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... and coated overnight at 4°C with anti-IFN-γ antibody (1-DIK, Mabtech). Plates were washed using PBS and then saturated with RPMI complemented with 10% FBS ...
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bioRxiv - Cancer Biology 2022Quote: ... IFN-y secretion was detected using IFN-γ capture and detection antibodies (MABtech technologies) assessed by ELISPOT assay following manufacturer instructions ...
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bioRxiv - Immunology 2021Quote: ... followed by application of IFN-γ-detection antibody (MABTECH Cat# 3420-6-250, RRID:AB_907273) and visualization by Streptavidin horseradish peroxidase (HRP ...
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bioRxiv - Immunology 2022Quote: ... wells were coated with human anti-IFN-γ monoclonal antibodies (1-D1K, Mabtech Sweden) at a concentration of 5μg/ml and diluted with the same coating buffer ...
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bioRxiv - Immunology 2022Quote: ... 100 µL of either goat anti-mouse IgG (for 6E2 hybridoma cells) or mouse anti-human IgG (for EBV-immortalized human B cells) specific for the Fc region (Mabtech) diluted in sterile PBS at 15 µg/mL were added to wells followed by incubation at 4°C for 24 hours ...
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bioRxiv - Microbiology 2020Quote: ... The stimulated splenocytes were analyzed for IFN-γ responses using the mouse IFN-γ ELISpot Plus kit (Mabtech AB, Sweden). Spot-forming cells (SFU ...
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bioRxiv - Immunology 2019Quote: ... NS1-specific IFNγ and IL-5 production by ZIKV-specific splenic T cells were enumerated by Enzyme Linked ImmunoSpot (ELISPOT) assay following manufacturer’s instructions (Mouse IFN-γ/IL-5 ELISpot Basic kits; Mabtech). Mouse splenocytes were left unstimulated or stimulated with an NS1 peptide pool (PepMix ZIKV NS1 Ultra ...
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bioRxiv - Immunology 2020Quote: IFN-γ ELISpot was performed on splenocytes of mice isolated after sacrifice using mouse IFN-γ ELISpot-plus kit (Mabtech). Splenocytes were obtained by disaggregation of spleens with the gentleMACS dissociator ...
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Efficacy of recombinant NP-M1 and NP-M1-CRT DNA vaccines against Influenza A viruses in mice C57/BL6bioRxiv - Molecular Biology 2021Quote: ... The number of NP-specific IFN□ secreting cells in mouse was counted using commercial ELISPOT assay kits (MABTECH AB, Sweden). This procedure was conducted according to the instruction manual ...
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bioRxiv - Immunology 2020Quote: ... the plates were developed with human biotinylated IFN-γ detection antibody (7-B6-1, MabTech), followed by incubation with Streptavidin-AP (MabTech ...
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bioRxiv - Immunology 2020Quote: ... were coated with human IFN-γ antibody (Clone 1-D1K, 2 μg/ml, Mabtech, Sweden) overnight at 4°C ...
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bioRxiv - Immunology 2021Quote: ... were coated with IFN-γ capture antibody at a concentration of 15 µg/mL (Mabtech). The following day ...
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bioRxiv - Immunology 2020Quote: ... the plates were developed with human biotinylated IFN-γ detection antibody (7-B6-1, Mabtech), followed by incubation with Streptavidin-AP (Mabtech ...
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bioRxiv - Immunology 2024Quote: ... and secondary antibodies (anti-BAM-490, anti-WASP-640, and SA-550, all from Mabtech) were then incubated for 1 hr at room temperature ...
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bioRxiv - Immunology 2024Quote: ... IFN-γ ELISPOTs were enumerated following development with an alkaline phosphatase-conjugated secondary antibody (Mabtech, clone 7-B6-1-ALP ...
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bioRxiv - Microbiology 2020Quote: Murine IFN-γ and IL-5 ELISPOT assays were performed following manufacturer’s procedures for mouse IFN-γ and IL-5 ELISPOT kit (Mabtech, Cincinnati, OH). Briefly ...
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bioRxiv - Immunology 2022Quote: Cellular immune responses in mice were performed using mouse IFN-γ/IL-4 ELISPOT PLUS plates (3321-4AST-2/3311-4APW-2, MABTECH, Sweden). 96-well ELISPOT plates were pre-treated as the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... ELISpot assays for IFNγ were performed as instructed by the manufacturer using the Mouse IFNγ ELISpot Set (MabTech, cat# Q00824-1×20210623DS). 0.1×106 mouse splenocytes were plated into each well and stimulated overnight with 0.2 µg per peptide per well of RBD-derived overlapping peptides (GenScript).
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bioRxiv - Immunology 2021Quote: Murine IFN-γ and IL-5 ELISpot assays were performed following the manufacturer’s procedures for mouse IFN-γ and IL-5 ELISpot kits (3321-2H and 3321-2A, Mabtech, Cincinnati, OH). Briefly ...
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bioRxiv - Immunology 2022Quote: Spleens from immunized animals were removed at Day 51 for the mice and at Day 110 for the hamsters and splenocytes were isolated for protein S-specific T-cell detection with the ELISpotPLUS mouse IFN-γ or ELISpotPLUS hamster IFN-γ kits (Mabtech) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... The ELISA was developed with the use of Goat anti-human IgG biotinylated antibody (Mabtech, Sweden), Streptavidin Alkaline Phosphatase enzyme (Abcam ...
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bioRxiv - Microbiology 2023Quote: ... that had been precoated with 5 µg/ml anti-IFN-γ monoclonal antibody (mAb) AN18 (Mabtech). The plates were incubated at 37 °C in 5 % CO2 for 18 h and washed with PBS before the addition of 1 µg/ml biotinylated anti-IFN-γ mAb (Mabtech ...
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bioRxiv - Microbiology 2023Quote: Interferon (IFN)-γ Enzyme-linked ImmunoSpot (ELISPOT) assay was performed as described previously160 using the Mouse IFN-γ ELISpot kit (Mabtech, Stockholm, Sweden) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... and ELISPOT plates were developed following the manufacturer’s protocol for the ELISpot Plus: Mouse IFN-γ (ALP) kit (Mabtech Cat. No. 3321-4APW). After the plates were developed ...
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bioRxiv - Immunology 2022Quote: ... 100 µl of biotinylated secondary anti-IFN-γ antibody (1 µg/ml, clone 7-B6-1, Mabtech) in blocking buffer (PBS 0.5% FBS ...
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bioRxiv - Immunology 2022Quote: ... the plates were developed with human biotinylated IFN-γ detection antibody (7-B6-1, Mabtech; 1:2000), followed by incubation with Streptavidin-AP (Mabtech ...
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bioRxiv - Immunology 2020Quote: ... 96 well plates were coated with a mixture of antibodies against IFN-α (Mabtech, MT1/3/5) overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Anti-IFN-γ coating antibody (10 µg/ml in PBS; clone 1-D1K; Mabtech, Nacka Strand, Sweden) was then added and the plates incubated overnight at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... 100 µl of biotinylated secondary anti-IFN-γ antibody (1 µg/ml, clone 7-B6-1, Mabtech) in blocking buffer (PBS 0.5% FBS ...
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bioRxiv - Immunology 2020Quote: ... and immediately washed 6x with 200μL ddH2O and then coated with 100μL/well of PBS + 15μg/mL primary anti-human granzyme B antibody (clone GB10, Mabtech) overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: ... were either directly coated with 100μL/well of PBS + 0.5μg/mL primary anti-human IFN-γ antibody (clone 1-D1K, Mabtech) overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: ELISpot plates were coated with 10 μg/mL anti-human IFN-γ coating antibody (Mabtech, clone 1-D1K) and incubated overnight at 4C° ...
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bioRxiv - Immunology 2020Quote: ... and 5 μg/mL anti-perforin capture antibody mix (capture αPrf; Mabtech, combination of clones Pf-80/164). The resulting surfaces were micropatterned as described before with 10 μg/mL αLFA-1 + 10 μg/mL αCD16 + 5 μg/mL capture αPrf + 10 μg/mL BSA-AF555 ...
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bioRxiv - Immunology 2023Quote: ... washed and coated overnight with anti-IFN-ψ coating antibody (mAb-1-D1K, Mabtech, Cat. 3420-3-1000). Plates were washed and T cells were co-cultured with autologous LCL in RPMI/ 10% FCS/ 1% Pen/Strep at an effector ...
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bioRxiv - Immunology 2022Quote: Single cell suspensions from lungs and spleens of mice were stimulated with mouse IL-2 (R & D Systems, Cat # 402-ML; 0.5 μg/mL) and RD848 (Mabtech, Cat # 3611-5X; 1 μg/mL) for 3 days to induce nonspecific polyclonal expansion ...
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bioRxiv - Immunology 2022Quote: ... and incubated for 2 h with an IFN-γ detection antibody (1 μg/ml; clone mAb-7B6-1; Mabtech) followed by 1 h incubation with AP-conjugated streptavidin (1:1000 in PBS ...
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bioRxiv - Immunology 2019Quote: ... and 1-2×105 cells were plated per well of a 96-well Millipore MAIPSWU plate coated with anti-IFNγ antibody according to the manufacturer’s instructions (3420-2HW-Plus, Mabtech Inc.). Peptide pools were added to the cells at a final concentration of 1μg/mL/peptide prior to incubation at 37°C overnight ...
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bioRxiv - Immunology 2020Quote: ... Cells were then discarded and plates were further incubated with biotinylated IFNγ antibody (Mab 7-B6-1; Mabtech, Stockholm, Sweden) and incubated for 2hr at 37 C° ...
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bioRxiv - Bioengineering 2022Quote: ... IL6 was captured to a level of 1300-2800 RU on an anti-IL6 monoclonal antibody (mAb clone 13A5, MABTECH) coupled to the chip surface and the interaction analysis of 4 selected Nbs was performed similarly as described above ...
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bioRxiv - Microbiology 2022Quote: ... and the plate was incubated for 2 h at room temperature with R4-6A2 biotinylated anti-IFN-γ antibody (Mabtech) at the concentration of 100 μg/mL ...
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bioRxiv - Immunology 2022Quote: ... Cells were removed and plates were then washed with PBS-0,05% Tween-20 prior incubation with biotinylated anti-IFN-γ antibody (7-B6-1, Mabtech) (2h ...
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bioRxiv - Immunology 2020Quote: ... cells were removed by washing (using PBS and PBS-Tween 0.02% solution) and 100 µl of biotinylated secondary anti-IFN-γ antibody (clone 7-B6-1, Mabtech) in blocking buffer (PBS 0.5% BSA ...
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bioRxiv - Immunology 2020Quote: ... ELISpot plates were coated with IFN-γ capture antibody (2 μg/ml per well, Mabtech mAb 1-D1K, 3420-7), in D-PBS and then blocked with RPMI-1640 supplemented media ...
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bioRxiv - Immunology 2021Quote: Antigen-specific cellular responses were measured by IFNγ Fluorospot assay with all antibodies and reagents from Mabtech (Nacka Strand, Sweden). Plates were coated overnight at 4°C with a mouse anti-human IFNγ (clone 1-D1K ...
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bioRxiv - Immunology 2020Quote: ... the plates were developed with human biotinylated IFN-γ detection antibody (Clone 7-B6-1, 1 μg/ml, Mabtech, Sweden), followed by incubation with Streptavidin-AP (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... Fluorescent spots indicating a single mASC and relative volume of antibody produced were detected with an IRIS FluoroSpot Reader and counted with Apex software (Mabtech). Positivity thresholds were set for both S1 and N mASCs based on prepandemic control samples’ average S1 or N plus three standard deviations.
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bioRxiv - Immunology 2023Quote: ... and the plate was incubated for 2 h at room temperature with R4-6A2 biotinylated anti-IFN-γ antibody (Mabtech) at the concentration of 100 μg/mL ...