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Citations for Mabtech :
151 - 200 of 203 citations for Mouse Anti Rift Valley Fever Virus Nucleoprotein Antibody AA11 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... ELISpot assays for IFNγ were performed as instructed by the manufacturer using the Mouse IFNγ ELISpot Set (MabTech, cat# Q00824-1×20210623DS). 0.1×106 mouse splenocytes were plated into each well and stimulated overnight with 0.2 µg per peptide per well of RBD-derived overlapping peptides (GenScript).
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bioRxiv - Immunology 2021Quote: Murine IFN-γ and IL-5 ELISpot assays were performed following the manufacturer’s procedures for mouse IFN-γ and IL-5 ELISpot kits (3321-2H and 3321-2A, Mabtech, Cincinnati, OH). Briefly ...
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bioRxiv - Immunology 2022Quote: Spleens from immunized animals were removed at Day 51 for the mice and at Day 110 for the hamsters and splenocytes were isolated for protein S-specific T-cell detection with the ELISpotPLUS mouse IFN-γ or ELISpotPLUS hamster IFN-γ kits (Mabtech) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... or monoclonal anti-human CD3 (Mabtech, Sweden, Cat. 3420-2AST-10) at a 1:1000 dilution for positive control wells ...
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bioRxiv - Immunology 2020Quote: ... Alkaline phosphatase conjugated anti-human IgG (clone MT78; Mabtech Stockholm, Sweden) and p-nitrophenyl phosphate substrate (Thermo Fisher ...
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bioRxiv - Microbiology 2023Quote: Interferon (IFN)-γ Enzyme-linked ImmunoSpot (ELISPOT) assay was performed as described previously160 using the Mouse IFN-γ ELISpot kit (Mabtech, Stockholm, Sweden) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... and ELISPOT plates were developed following the manufacturer’s protocol for the ELISpot Plus: Mouse IFN-γ (ALP) kit (Mabtech Cat. No. 3321-4APW). After the plates were developed ...
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bioRxiv - Immunology 2022Quote: ... the plates were developed with human biotinylated IFN-γ detection antibody (7-B6-1, Mabtech; 1:2000), followed by incubation with Streptavidin-AP (Mabtech ...
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bioRxiv - Immunology 2020Quote: ... 96 well plates were coated with a mixture of antibodies against IFN-α (Mabtech, MT1/3/5) overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: ... 50 μL/well of biotinylated anti-IgG mAb (clone MT424-biotin, Mabtech) was added at 0.5 µg/mL ...
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bioRxiv - Immunology 2022Quote: Single cell suspensions from lungs and spleens of mice were stimulated with mouse IL-2 (R & D Systems, Cat # 402-ML; 0.5 μg/mL) and RD848 (Mabtech, Cat # 3611-5X; 1 μg/mL) for 3 days to induce nonspecific polyclonal expansion ...
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bioRxiv - Genomics 2020Quote: ... Cells were stimulated with soluble anti-CD3 (5 μg/mL; MabTech, Cincinnati, OH) and anti-CD28 antibodies (10ug/mL ...
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bioRxiv - Immunology 2020Quote: ... pre-coated with anti-IFN-γ (clone 1-D1K; 15 μg/ml; Mabtech). Test wells were supplemented with overlapping peptides spanning Prot_E ...
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bioRxiv - Immunology 2023Quote: ... were coated with anti-human IFN-γ mAb 1-D1K (Mabtech, Cincinnati, OH) diluted 1:400 in PBS (Gibco ...
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bioRxiv - Immunology 2022Quote: ... and incubated for 2 h with an IFN-γ detection antibody (1 μg/ml; clone mAb-7B6-1; Mabtech) followed by 1 h incubation with AP-conjugated streptavidin (1:1000 in PBS ...
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bioRxiv - Immunology 2020Quote: ... Cells were then discarded and plates were further incubated with biotinylated IFNγ antibody (Mab 7-B6-1; Mabtech, Stockholm, Sweden) and incubated for 2hr at 37 C° ...
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bioRxiv - Immunology 2020Quote: ... ELISpot plates were coated with IFN-γ capture antibody (2 μg/ml per well, Mabtech mAb 1-D1K, 3420-7), in D-PBS and then blocked with RPMI-1640 supplemented media ...
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bioRxiv - Immunology 2021Quote: Antigen-specific cellular responses were measured by IFNγ Fluorospot assay with all antibodies and reagents from Mabtech (Nacka Strand, Sweden). Plates were coated overnight at 4°C with a mouse anti-human IFNγ (clone 1-D1K ...
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bioRxiv - Immunology 2020Quote: ... the plates were developed with human biotinylated IFN-γ detection antibody (Clone 7-B6-1, 1 μg/ml, Mabtech, Sweden), followed by incubation with Streptavidin-AP (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... Fluorescent spots indicating a single mASC and relative volume of antibody produced were detected with an IRIS FluoroSpot Reader and counted with Apex software (Mabtech). Positivity thresholds were set for both S1 and N mASCs based on prepandemic control samples’ average S1 or N plus three standard deviations.
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bioRxiv - Immunology 2024Quote: ... were pre-coated with 15 μg/ml capturing monoclonal anti-human IgG mAbs (Mabtech) and incubated overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: IFNγ and IL-17 production was measured by a Fluorospot assay with all antibodies and reagents from Mabtech (Nacka Strand, Sweden). Plates were coated overnight at 4°C with an antibody mixture containing mouse anti-human IFNγ (clone 1-D1K ...
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bioRxiv - Cancer Biology 2023Quote: ... were hydrophilized with 35 % EtOH and subsequently coated with anti-human IFNγ (1-D1K, Mabtech). Blocking was performed with X-Vivo-20 (Lonza ...
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bioRxiv - Immunology 2021Quote: ... cells were discarded and FluoroSpot plates were washed and further incubated for 2 hours with cytokine antibodies (mAbs 7-B6-1-BAM; Mabtech, Stockholm, Sweden). Subsequently ...
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bioRxiv - Immunology 2020Quote: ... cells were discarded and FluoroSpot plates were washed and further incubated for 2hr with cytokine antibodies (mAbs 7-B6-1-BAM, 5A10 biotinylated, MT20D9-WASP, respectively; Mabtech, Stockholm, Sweden). Subsequently ...
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bioRxiv - Immunology 2020Quote: ... The plate was washed and IFN-I assayed using a mixture of biotinylated detection antibodies against IFN-α (Mabtech, MT2/4/6). Avidin-conjugated alkaline phosphatase (Sigma ...
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bioRxiv - Immunology 2021Quote: ... cells were discarded and plates were washed before the addition of cytokine antibodies (mAbs 7-B6-1-BAM and 5A10-WASP; Mabtech, Stockholm, Sweden). After a 2-hour incubation ...
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bioRxiv - Cancer Biology 2021Quote: ... Plates were developed by washing 6 times in PBS/0.05%Tween 20 followed by incubation with biotinylated IFN-γ detection antibody (0.5 mg/mL; Mabtech; 2 hours, 37°C). This was followed by a further 6 washes in PBS/0.05%Tween 20 and incubation with avidin DH-coupled biotinylated peroxidase H complex (Vectastain Elite ABC Kit ...
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bioRxiv - Immunology 2022Quote: ... with 40 μg/mL anti-human IFNγ mAb 1-D1K (MABTECH, Stockholm, Sweden, Cat #3420-3-250) for 30 minutes followed by two washes ...
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bioRxiv - Immunology 2020Quote: ... and incubated with 50 μL of biotinylated anti-porcine IFN-γ mAb P2C11 (1:1000 dilution; Mabtech) after overnight incubation ...
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bioRxiv - Immunology 2020Quote: ... were coated with 100 μL of 15 μg/mL anti-porcine IgG mAb (clone MT421, Mabtech, 2BScientific) diluted in 0.05M carbonate-bicarbonate buffer pH 9.2 (Merck) ...
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bioRxiv - Immunology 2020Quote: ... The following morning plates were washed with 1x PBS-T before being coated with 3 μg/mL streptavidin-ALP secondary antibody (Mabtech, clone 7-B6-1) and incubated for 2 hours at room temperature ...
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bioRxiv - Immunology 2020Quote: ... plates were coating with 50 μL of anti-porcine IFN-γ mAb pIFNγ-I (5 µg/ ml; Mabtech) and incubated with 50 μL of biotinylated anti-porcine IFN-γ mAb P2C11 (1:1000 dilution ...
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bioRxiv - Immunology 2019Quote: ... then added with alkaline phosphatase (ALP) labeled anti-human IFN-γ mAb (7-B6-1-ALP, 1:200; Mabtech) for 2 hours ...
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bioRxiv - Immunology 2019Quote: IFN-γ ELISPOT assay strip plate which was pre-coated with anti-human IFN-γ mAb (1-D1K, Mabtech) was washed with PBS and blocked with RPMI-1640 containing 10% FBS ...
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bioRxiv - Bioengineering 2021Quote: ... and wells incubated with 100 µL of 1 µg/ml of the R4-6A2 biotinylated anti-IFN-γ (Mabtech) for 2 hours at r.t ...
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bioRxiv - Immunology 2020Quote: ... Antigen-specific IgG was detected by addition of alkaline phosphatase-conjugated anti-human IgG (clone MT78; Mabtech, Cincinnati, OH), followed by p-nitrophenyl phosphate substrate ...
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bioRxiv - Immunology 2020Quote: ... and wells incubated with 100 μL of 1 μg/ml of the R4-6A2 biotinylated anti-IFN-γ (Mabtech) for 2 hours at r.t ...
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bioRxiv - Immunology 2021Quote: ... Reactivity was assessed by specific IFN-γ release by ELISpot assay by coating ELISpot plates (MAHAS4510) with IFN-γ capture antibody (MABTECH Cat# 3420-3-250, RRID:AB_907283) followed by application of IFN-γ-detection antibody (MABTECH Cat# 3420-6-250 ...
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bioRxiv - Cancer Biology 2021Quote: ... and the wells incubated with 100 μL of 1 μg/ml of the R4-6A2 biotinylated anti-IFN-γ (Mabtech) for 2 hours at r.t ...
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bioRxiv - Immunology 2020Quote: ... before incubation with 100 µl per well of 0.5 µg/ml biotinylated anti porcine IgG (mAb, MT424, Mabtech AB, Sweden) diluted in PBS/0.5 % FBS for two hours at room temperature ...
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bioRxiv - Immunology 2020Quote: ... before incubation with 100 µl per well of 0.5 µg/ml biotinylated anti porcine IgG (mAb, MT424, Mabtech AB, Sweden) diluted in PBS/0.5 % FBS for two hours at room temperature ...
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bioRxiv - Immunology 2022Quote: ... we washed off the cells and developed the plates using biotinylated R4-6A2 anti-IFN-γ (Mabtech #3321-6-250) and BVD6-24G2 anti-IL4 (Mabtech #3311-6-250 ...
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bioRxiv - Immunology 2019Quote: Total splenocytes were cultured on anti-IgG ELISpot plates and developed using the standardized ELISpot kit (Mabtech Inc., Cincinnati, OH, USA). Briefly ...
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bioRxiv - Immunology 2022Quote: ... the ELISPOT plates were washed and stained with anti-IFN-γ-biotin followed by streptavidin-conjugated HRP (3420-2HW-Plus, Mabtech). Plates were developed using TMB substrate and read using a CTL-ImmunoSpot® S6 Analyzer (Cellular Technology Limited ...
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bioRxiv - Immunology 2020Quote: ... and incubated for 2 hr at room temperature with biotinylated anti-IFN-γ (clone mAb-7B6-1; 1 μg/ml Mabtech). After six further washes ...
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bioRxiv - Immunology 2021Quote: ... followed by two washes with PBS and then incubated for 2 hr at RT with biotinylated anti-IFN-γ (1 μg/mL; clone mAb-7B6-1; Mabtech). After six further washes ...
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bioRxiv - Microbiology 2023Quote: ... The plates were incubated at 37 °C in 5 % CO2 for 18 h and washed with PBS before the addition of 1 µg/ml biotinylated anti-IFN-γ mAb (Mabtech) at room temperature for 2 h ...
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bioRxiv - Immunology 2022Quote: Incubate the array with 10 μg/mL biotinylated anti-human IFNγ 7-B6-1 (MABTECH AB, Stockholm, Sweden, Cat #3420-6-250) and then 12 μg/mL AF647-Streptavidin (Jackson ImmunoResearch ...
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bioRxiv - Immunology 2020Quote: ... 5% CO2 at a concentration of 1×105 cells/mL media on plates previously coated with anti-human IFNγ (Mab 1-D1K; Mabtech, Stockholm, Sweden). Cells were then discarded and plates were further incubated with biotinylated IFNγ antibody (Mab 7-B6-1 ...