Labshake search
Citations for Mabtech :
51 - 100 of 209 citations for Mouse Anti Cytomegalovirus Glycoprotein B Antibody 0811 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2021Quote: ... Plates contained additional no target cell negative control and anti-CD3 antibody (CD3-2, Mabtech) positive control wells ...
-
bioRxiv - Immunology 2019Quote: ... The ELISA was developed with the use of Goat anti-human IgG biotinylated antibody (Mabtech, Sweden), Streptavidin Alkaline Phosphatase enzyme (Abcam ...
-
bioRxiv - Microbiology 2023Quote: ... that had been precoated with 5 µg/ml anti-IFN-γ monoclonal antibody (mAb) AN18 (Mabtech). The plates were incubated at 37 °C in 5 % CO2 for 18 h and washed with PBS before the addition of 1 µg/ml biotinylated anti-IFN-γ mAb (Mabtech ...
-
bioRxiv - Immunology 2020Quote: ... were pre-wet with 70% methanol and then coated with 100 µl primary anti-IFN-γ antibody solution (10 µg/ml of 1-D1K coating antibody from Mabtech in PBS, pH 7.4, V-E4) OVN at 4°C ...
-
bioRxiv - Immunology 2022Quote: ... 100 µl of biotinylated secondary anti-IFN-γ antibody (1 µg/ml, clone 7-B6-1, Mabtech) in blocking buffer (PBS 0.5% FBS ...
-
bioRxiv - Immunology 2022Quote: ... Anti-IFN-γ coating antibody (10 µg/ml in PBS; clone 1-D1K; Mabtech, Nacka Strand, Sweden) was then added and the plates incubated overnight at 4 °C ...
-
bioRxiv - Immunology 2024Quote: ... 100 µl of biotinylated secondary anti-IFN-γ antibody (1 µg/ml, clone 7-B6-1, Mabtech) in blocking buffer (PBS 0.5% FBS ...
-
bioRxiv - Immunology 2020Quote: ... IFN-γ spot forming cells (SFC) were detected by staining membranes with anti-mouse IFN-γ biotin mAb (1 µg/mL; clone R46A2, Mabtech) followed by streptavidin-alkaline phosphatase (1 µg/mL ...
-
bioRxiv - Immunology 2020Quote: ... were either directly coated with 100μL/well of PBS + 0.5μg/mL primary anti-human IFN-γ antibody (clone 1-D1K, Mabtech) overnight at 4°C ...
-
bioRxiv - Immunology 2020Quote: ELISpot plates were coated with 10 μg/mL anti-human IFN-γ coating antibody (Mabtech, clone 1-D1K) and incubated overnight at 4C° ...
-
bioRxiv - Immunology 2020Quote: ... and 5 μg/mL anti-perforin capture antibody mix (capture αPrf; Mabtech, combination of clones Pf-80/164). The resulting surfaces were micropatterned as described before with 10 μg/mL αLFA-1 + 10 μg/mL αCD16 + 5 μg/mL capture αPrf + 10 μg/mL BSA-AF555 ...
-
bioRxiv - Immunology 2023Quote: ... washed and coated overnight with anti-IFN-ψ coating antibody (mAb-1-D1K, Mabtech, Cat. 3420-3-1000). Plates were washed and T cells were co-cultured with autologous LCL in RPMI/ 10% FCS/ 1% Pen/Strep at an effector ...
-
bioRxiv - Immunology 2021Quote: ... (MabTech, Mouse IFNγ ELISpot Plus). Cells were washed off ...
-
bioRxiv - Immunology 2020Quote: ... The detection antibody solution composed of PBS + 5 μg/mL anti-perforin conjugated with biotin (clone Pf-344, Mabtech) was added to the slide chambers ...
-
bioRxiv - Immunology 2021Quote: ... cryopreserved PBMCs from study subjects were stimulated for 5 days with polyclonal B cell stimulation (1μg/mL R848 and 10 ng/mL rIL-2, Mabtech) in R10 media (RPMI 1640 with 10% FCS ...
-
bioRxiv - Immunology 2019Quote: ... and 1-2×105 cells were plated per well of a 96-well Millipore MAIPSWU plate coated with anti-IFNγ antibody according to the manufacturer’s instructions (3420-2HW-Plus, Mabtech Inc.). Peptide pools were added to the cells at a final concentration of 1μg/mL/peptide prior to incubation at 37°C overnight ...
-
bioRxiv - Bioengineering 2022Quote: ... IL6 was captured to a level of 1300-2800 RU on an anti-IL6 monoclonal antibody (mAb clone 13A5, MABTECH) coupled to the chip surface and the interaction analysis of 4 selected Nbs was performed similarly as described above ...
-
bioRxiv - Microbiology 2022Quote: ... and the plate was incubated for 2 h at room temperature with R4-6A2 biotinylated anti-IFN-γ antibody (Mabtech) at the concentration of 100 μg/mL ...
-
bioRxiv - Immunology 2022Quote: ... Cells were removed and plates were then washed with PBS-0,05% Tween-20 prior incubation with biotinylated anti-IFN-γ antibody (7-B6-1, Mabtech) (2h ...
-
bioRxiv - Immunology 2020Quote: ... cells were removed by washing (using PBS and PBS-Tween 0.02% solution) and 100 µl of biotinylated secondary anti-IFN-γ antibody (clone 7-B6-1, Mabtech) in blocking buffer (PBS 0.5% BSA ...
-
bioRxiv - Immunology 2023Quote: ... and the plate was incubated for 2 h at room temperature with R4-6A2 biotinylated anti-IFN-γ antibody (Mabtech) at the concentration of 100 μg/mL ...
-
Metabolomics reveals nucleoside analogs for regulating mucosal-associated invariant T cell responsesbioRxiv - Immunology 2023Quote: ... The IFNγ+ MAIT cell spots were then developed with an indirect immunostain approach sequentially using a biotinylated anti-human IFNγ antibody (Mabtech), ExtraAvidin conjugated by alkaline phosphatase (Sigma) ...
-
bioRxiv - Immunology 2021Quote: ... and IgA production by each sorted B-cell subset were evaluated by using the Human IgG/IgM and IgG/IgA FluoroSpot kits (MABTECH), according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: ... IgG- and IgM-ASCs in freshly isolated PBMCs were measured using a multicolor B cell FluoroSpot kit with modifications (Mabtech). Briefly ...
-
Dendritic cells focus CTL responses toward highly conserved and topologically important HIV epitopesbioRxiv - Immunology 2020Quote: ... T cells were resuspended at a concentration of 3×105 cells per milliliter and 100 μl (3×104 cells/well) were transferred to IFNγ-antibody coated 96-well ELISpot plates (5μg/ml anti-IFNγ mAb 1-D1K, Mabtech, Stockholm, Sweden). Individual 9mer peptide dilutions were done in a separate 1 ml deep-well 96-well plate at a final concentration of 2μg/ml and 100 μl of this dilution was added to T cell-containing wells to give a final peptide concentration of 1μg/ml ...
-
bioRxiv - Immunology 2019Quote: ... was coated overnight at 4 °C with 10 μg ml−1solution of anti-IFN-γ monoclonal antibody (Mabtech clone 1-D1K) in a buffer solution of 0.1 M Na2CO3 ...
-
bioRxiv - Immunology 2022Quote: ... followed by 2 hours of incubation at 37°C with biotinylated anti-human IFN-γ monoclonal antibodies (clone 7-B6-1, Mabtech) diluted at 1μg/ml in PBS ...
-
bioRxiv - Immunology 2021Quote: Mouse IL-2 (MABTECH, 3441-4APW-2), IL-4 (MABTECH ...
-
bioRxiv - Microbiology 2022Quote: IL-4 and IL-2 ELISPOT assays were performed with mouse IL-4 ELISPOTPLUS kits and mouse IL-2 ELISPOTPLUS kits according to the manufacturer’s instructions (Mabtech). IFN-γ ELISPOT assays were performed with mouse IFN-γ Precoated ELISPOT kits according to the manufacturer’s instructions (Dakewe Biotech) ...
-
bioRxiv - Immunology 2020Quote: ... Controls included 1×105 effector CD8+ T cells co-cultured with anti-human CD3 monoclonal antibody (0.5 µg/mL, Mabtech, 3605-1-1000), or with media alone ...
-
bioRxiv - Immunology 2021Quote: 96-well FluoroSpot plates were coated with anti-cytokine antibodies for IFNγ and IL-5 (mAbs 1-D1K and TRFK5, respectively; Mabtech, Stockholm, Sweden) at a concentration of 10μg/mL ...
-
bioRxiv - Immunology 2023Quote: ... mouse IFN-γ/IL-5 (X-41A43B; Mabtech) and IFN-γ/IL-2/TNF-α (X-41A42B45W ...
-
bioRxiv - Immunology 2022Quote: IFNγ and IL4 ELISpot assay was performed using Mouse IFNγ ELISPOT basic kit (ALP) and Mouse IL4 ELISPOT basic kit following the manufacturer’s instructions (Mabtech, VA, USA). For cell activation control ...
-
bioRxiv - Immunology 2021Quote: ... washed with sterile PBS and coated overnight at 4 °C with anti–IFN-γ antibody (10 µg/ml in PBS; clone 1-D1K; Mabtech, Nacka Strand, Sweden). Prior to use ...
-
bioRxiv - Cancer Biology 2023Quote: ... and Granzyme B (Biozol, #3485-1H-20 for CD276- and CD19-CAR T cells; MabTech, #3486-1H-6 for F8-FR4-CAR T cells) following the manufactures’ instructions ...
-
bioRxiv - Immunology 2023Quote: ... Wells were washed and incubated for 2 hours with biotinylated anti-IFN-ψ detection antibody (mAb-7-B6-1-Biotin, Mabtech, Cat. 3420-6-250). Afterwards ...
-
bioRxiv - Cancer Biology 2021Quote: ... Mouse IFN-γ ELISpot PLUS kit (MABTECH 3321-4APT-2) manufacturer protocol was followed for development of the spots ...
-
bioRxiv - Microbiology 2019Quote: ... an enzyme-linked immunosorbent assay kit detecting mouse IgG (Mabtech) was used ...
-
bioRxiv - Immunology 2023Quote: ... Ninety six-well mouse IFN-γ ELISPOT kit (Mabtech, USA) plates pre-coated with anti-mouse IFN-γ capture antibody were rinsed 5 times with PBS to hydrate the membrane and then blocked with supplemented CTL media for at least 30 minutes ...
-
bioRxiv - Immunology 2020Quote: ... then wells were washed 5 times with sterile D-PBS and incubated with anti–IFNγ biotinylated antibody (1 µg/mL per well, Mabtech, mAb 7-B6-1, 3420-9H) for 2 hours.
-
bioRxiv - Immunology 2019Quote: ... and perforin (antibody sets from MABTECH), using ELISA substrate ADHP from Cayman Chemical ...
-
bioRxiv - Bioengineering 2021Quote: ... pre-coated with the AN18 mAb against mouse IFN-γ (Mabtech) in RPMI 1640 (Gibco) ...
-
bioRxiv - Cancer Biology 2021Quote: ... pre-coated with the AN18 mAb against mouse IFN-γ (Mabtech) in RPMI 1640 (Gibco ...
-
bioRxiv - Immunology 2020Quote: ... pre-coated with the AN18 mAb against mouse IFN-γ (Mabtech) in RPMI 1640 (Gibco) ...
-
bioRxiv - Neuroscience 2023Quote: ... Mouse IL-17A ELISpotPLUS kit ALP (MABTECH, Cat# 3521-4APW-2) was performed following manufacturer’s instructions ...
-
bioRxiv - Immunology 2020Quote: ... were coated with 1D1K antibody (Mabtech, Sweden) diluted 1:400 in PBS and incubated overnight at 4°C ...
-
bioRxiv - Immunology 2023Quote: ... IFN-γ capturing antibody (MABTECH,3321-2H) was diluted to 15ug/ml with PBS and added to the above plates at 100ul/well and the plates were incubated at 4°C overnight ...
-
bioRxiv - Immunology 2021Quote: ... anti-IgM (Mabtech) or anti-IgG (Mabtech) ...
-
bioRxiv - Immunology 2020Quote: ... biotinylated antibody (Mabtech, Sweden Cat: 3820-4-250) was diluted 1:1000 in PBS containing 1% BSA and added 100μl/well ...
-
bioRxiv - Immunology 2021Quote: Precoated monoclonal antibody IL-4 ELISPOT plates (Mabtech) were washed and blocked ...