Labshake search
Citations for Mabtech :
51 - 100 of 120 citations for IL 9 Mouse CHO since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... IL-2 (Cy3) and/or TNF (Cy5) were detected with IRIS FluoroSpot reader and counted with Apex software (Mabtech). Numbers of responding cells in negative controls were subtracted from stimulated samples to account for background responses ...
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bioRxiv - Bioengineering 2024Quote: ... Anti-mouse IgG capture antibody (Mabtech) diluted to 15 µg/mL in PBS was then added to each well before incubation overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... PBMC were thawed and cultured at 37°C and 5% CO2 in the presence of 1 μg/mL R848 and 10 ng/mL of recombinant interleukin (IL) 2 (both from Mabtech).
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bioRxiv - Immunology 2020Quote: IL-6 levels in serum from patients and healthy controls were measured in freshly thawed serum using human IL-6 ELISA development kit (Mabtech), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... the 2CZ T cell hybridoma was used and the IL-2 secreted upon its activation was quantified using an ELISPOT assay (Mabtech) as previously described 7.
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bioRxiv - Immunology 2023Quote: ... Commercially available ELISPOT plates for evaluation of IL-4 (MuIL4, Immunospot, Cellular Technology Limited) and IFNg (MuIFNg, Immunospot, Cellular Technology Limited; cat# 3110-4APW-10, rabbit, MabTech) were used ...
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bioRxiv - Microbiology 2024Quote: ... Interferon-gamma (IFN-γ) and interleukin-4 (IL-4) were quantified with commercially-available kits (MABTECH AB and FineTest, respectively) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... Secreted mouse IFN-γ was captured by anti-mouse IFN-γ (AN18) monoclonal antibody (Mabtech, Nacka Strand, Sweden) precoated on 96-well nitrocellulose membrane plates (Merk Millipore ...
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bioRxiv - Immunology 2022Quote: The T cell immune responses in rhesus macaques were detected using the PBMCs with commercially available NHP IFN-γ and IL-2 ELISpot assay kits (Mabtech), and NHP IL-4 ELISpot assay kit (U-CyTech) ...
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bioRxiv - Immunology 2020Quote: IFN-γ and IL-2 secreting T cells were detected using Human IFN-γ/IL-2 SARS-CoV-2 FluoroSpotPLUS kits according to the manufacture’s protocol (Mabtech AB). The plates pre-coated with capturing monoclonal anti-IFN-γ and anti-IL-2 were incubated overnight in RPMI-1640 medium containing 10% FCS supplemented with a mixture containing the SARS-CoV-2 peptide pool (scanning or defined pools) ...
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bioRxiv - Microbiology 2021Quote: ... at an E:T ratio of 5:1 for 24 hours followed by measurement of specific cytokine production using the human IL-2 ELISpot kit (Mabtech AB) and ELIPHOTO Counter (Minerva Tech).
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bioRxiv - Immunology 2021Quote: To assess memory T cell responses to SARS-CoV-2 in convalescent COVID-19 patient samples we utilized Human IFN-γ/IL-2/TNF FluoroSpot kit (Mabtech), which allows for the detection of T cells producing IFN-γ ...
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bioRxiv - Bioengineering 2023Quote: ... were collected for ELISpot assays using ELISpot Plus: Monkey IFN-γ (HRP) and ELISpot Plus: Human IL-4 (HRP) kits (Mabtech). 2 × 105 PBMCs seeded onto 96 well plates in the kits were incubated with 1 μg of PepMixTM SARS-CoV-2 (Spike Glycoprotein ...
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bioRxiv - Immunology 2023Quote: IFNγ and IL-17 production was measured by a Fluorospot assay with all antibodies and reagents from Mabtech (Nacka Strand, Sweden). Plates were coated overnight at 4°C with an antibody mixture containing mouse anti-human IFNγ (clone 1-D1K ...
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bioRxiv - Immunology 2021Quote: ... splenocytes or lung leukocytes were stimulated with 1 μg/ml R848 and 10 ng/ml recombinant human IL-2 (Mabtech In, OH). Millipore ELISPOT plates (Millipore Ltd ...
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bioRxiv - Immunology 2021Quote: 96-well FluoroSpot plates were coated with anti-cytokine antibodies for IFNγ and IL-5 (mAbs 1-D1K and TRFK5, respectively; Mabtech, Stockholm, Sweden) at a concentration of 10μg/mL ...
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bioRxiv - Immunology 2021Quote: ELISpot assays were performed with mouse IFN-γ and IL-4 ELISpot PLUS kits according to the manufacturer’s instructions (3321-4HPT-10 and 3311-4HPW-10, Mabtech, Herndon, VA, USA). A total of 2,5 × 105 or 4 x 105 splenocytes from female mice were seeded per well for the IFN-γ and IL-4 tests respectively and ex vivo stimulated either with the 1:1 mix of the RBD overlapping peptides from the B.1.1.7 (alpha variant ...
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bioRxiv - Immunology 2022Quote: IFNγ and IL4 ELISpot assay was performed using Mouse IFNγ ELISPOT basic kit (ALP) and Mouse IL4 ELISPOT basic kit following the manufacturer’s instructions (Mabtech, VA, USA). For cell activation control ...
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bioRxiv - Immunology 2022Quote: ... kits with pre-coated plates for enumeration of cells secreting IFN-γ and IL-17A were purchased from Mabtech (Cat. No. FSP-414443-2). Spots were enumerated on an AID iSpot EliSpot/ FluoroSpot Reader.
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bioRxiv - Cancer Biology 2021Quote: ... Mouse IFN-γ ELISpot PLUS kit (MABTECH 3321-4APT-2) manufacturer protocol was followed for development of the spots ...
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bioRxiv - Microbiology 2019Quote: ... an enzyme-linked immunosorbent assay kit detecting mouse IgG (Mabtech) was used ...
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bioRxiv - Immunology 2023Quote: ... Ninety six-well mouse IFN-γ ELISPOT kit (Mabtech, USA) plates pre-coated with anti-mouse IFN-γ capture antibody were rinsed 5 times with PBS to hydrate the membrane and then blocked with supplemented CTL media for at least 30 minutes ...
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bioRxiv - Immunology 2021Quote: ... followed by incubation with biotin-conjugated anti-mouse IgG (Mabtech In) for 2 h at room temperature ...
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bioRxiv - Immunology 2021Quote: ... goat anti-mouse IgA biotin (Mabtech, Cat no. 3865-6-250) at (1:500 ...
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bioRxiv - Bioengineering 2021Quote: ... pre-coated with the AN18 mAb against mouse IFN-γ (Mabtech) in RPMI 1640 (Gibco) ...
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bioRxiv - Microbiology 2022Quote: ... previously coated with the anti-mouse IFN-γ AN18 mAb (Mabtech) in RPMI 1640 ...
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bioRxiv - Cancer Biology 2021Quote: ... pre-coated with the AN18 mAb against mouse IFN-γ (Mabtech) in RPMI 1640 (Gibco ...
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bioRxiv - Immunology 2020Quote: ... coated with 5μg/ml anti-mouse IFN-γ (clone AN18; Mabtech). After 18-20 hours of stimulation ...
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bioRxiv - Immunology 2020Quote: ... pre-coated with the AN18 mAb against mouse IFN-γ (Mabtech) in RPMI 1640 (Gibco) ...
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bioRxiv - Cancer Biology 2022Quote: ... IFNΨ secretion was assessed biotinylated anti-mouse IFNΨ (R4-6A2; Mabtech) and imaged using an ELISPOT reader (AID Diagnostika) ...
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bioRxiv - Microbiology 2023Quote: ... followed by incubation with biotin-conjugated anti-mouse IgA (Mabtech In) for 2 h at room temperature ...
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bioRxiv - Immunology 2021Quote: ... ELISpotPLUS plates as well as Mouse IFNγ ELISpotPRO plates (MABTECH, 3421M-2AST-2) were washed with PBS and blocked overnight at 4°C in RPMI 1640 media (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... previously coated with the anti-mouse IFN-AN18 mAb (Mabtech, Nacka Strand, Sweden) in RPMI 1640 ...
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bioRxiv - Immunology 2022Quote: Interferon gamma production was measured using the ELISpot Plus: Mouse IFN-γ (ALP) (Mabtech). Splenocytes were counted and 250,000 cells were plated per well into pre-covered plates and stimulated overnight with 10 μg/mL SARS-CoV-2 (S1 ...
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bioRxiv - Immunology 2023Quote: ... ELISpot was performed using mouse IFN-γ ELISpot PLUS kits (MABTECH, Cincinnati, OH, USA) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ELISpot assays were performed with mouse IFNγ ELISpotPLUS kits according to the manufacturer’s instructions (Mabtech). A total of 5 × 105 splenocytes was ex vivo restimulated with the full-length S peptide mix (0.1 µg/mL final concentration per peptide ...
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bioRxiv - Immunology 2022Quote: ... IFNγ ELISPOT was performed using the Mouse IFNγ ELISPOTPLUS kit (MABTECH product # 3321-4HPW-10). ELISPOT plates were analyzed using ImmunoSpot CTL plate reader (CTL ImmunoSpot S6 Entry Analyzer ...
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bioRxiv - Immunology 2019Quote: ... were coated at 4°C overnight with anti-mouse IFN-γ mAb AN-18 (Mabtech), at 250 ng per well ...
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bioRxiv - Immunology 2020Quote: ... Antigen-specific IgA titers in lungs were detected using a mouse IgA ELISA kit (Mabtech) and pNPP substrate (Mabtech) ...
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bioRxiv - Immunology 2022Quote: ... Cells were plated in the Mouse IFN-γ ELISPOTPLUS kit (ALP) (MABTECH, Nacka Strand, Sweden) and IFN-γ spots were detected after 48 h using ELISPOT reader systems (Autoimmun Diagnostika GmbH ...
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bioRxiv - Microbiology 2023Quote: ... the wells were coated with 100 µl of anti-mouse IgA capture Ab (Mabtech In). Cells were added in duplicates to assess total IgA ASCs or SARS-CoV-2 specific B cells ...
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bioRxiv - Immunology 2020Quote: Murine ELISpot assays were performed with mouse IFNγ ELISpotPLUS kits according to the manufacturer’s instructions (Mabtech). A total of 5 × 105 splenocytes was ex vivo restimulated with the full-length S peptide mix (0.1 μg/mL final concentration per peptide ...
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bioRxiv - Immunology 2020Quote: ... were coated with 50 μl of anti-mouse IFN-γ mAb AN18 (5 µg/ ml; Mabtech) overnight at 4°C before being washed and blocked with cRMPI 10% FBS for 1 hr at 37C ...
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bioRxiv - Immunology 2022Quote: IFN-γ ELISPOT assay was performed using a Mouse IFN-γ ELISpot antibody pair (Mabtech, Sweden). Splenocytes were isolated in RPMI culture medium (Gibco ...
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bioRxiv - Immunology 2022Quote: ... Spot-forming units (SFU) were quantified using a mouse IFN-γ ELISpot kit (Mabtech, Stockholm, Sweden) per the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... the wells were coated with 100 μL of anti-mouse IgG or IgA capture Ab (Mabtech In). Stimulated cells were harvested ...
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bioRxiv - Immunology 2020Quote: ... then washed with PBS-Tween 0.01% before to add anti-mouse IgG antibody (Mabtech – 1/500 dilution). After the last washing ...
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bioRxiv - Immunology 2023Quote: ... 96-well nitrocellulose-bottom plates pre-coated with anti-mouse IFNγ monoclonal antibody were purchased from Mabtech. The plates were blocked with RPMI-10% FBS for at least 30 minutes ...
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bioRxiv - Immunology 2023Quote: ... and incubated with 1 μg/ml of the biotinylated anti-mouse IFNγ monoclonal antibody R4-6A2 (Mabtech) diluted in PBS-0.5% FCS for 2 h ...
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bioRxiv - Immunology 2024Quote: ... then incubated at room temperature with biotinylated secondary goat anti-mouse IgG (catalogue #3825-6-250, Mabtech) at a 2 µg/mL concentration in the dark for 2 hours ...