Labshake search
Citations for Mabtech :
101 - 150 of 219 citations for IL 1 beta Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... Antigen-specific IgG ELISpot assays were performed using Human IgG ELISpotBASIC kit (MabTech). PBMCs were first pre-stimulated with a mixture of R848 at 1 μg/ml and rhIL-2 at 10 ng/ml for 96h ...
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bioRxiv - Immunology 2022Quote: The T cell immune responses in rhesus macaques were detected using the PBMCs with commercially available NHP IFN-γ and IL-2 ELISpot assay kits (Mabtech), and NHP IL-4 ELISpot assay kit (U-CyTech) ...
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bioRxiv - Immunology 2023Quote: IFNγ and IL-17 production was measured by a Fluorospot assay with all antibodies and reagents from Mabtech (Nacka Strand, Sweden). Plates were coated overnight at 4°C with an antibody mixture containing mouse anti-human IFNγ (clone 1-D1K ...
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bioRxiv - Immunology 2022Quote: Cellular immune responses in mice were performed using mouse IFN-γ/IL-4 ELISPOT PLUS plates (3321-4AST-2/3311-4APW-2, MABTECH, Sweden). 96-well ELISPOT plates were pre-treated as the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Murine IFN-γ and IL-5 ELISpot assays were performed following the manufacturer’s procedures for mouse IFN-γ and IL-5 ELISpot kits (3321-2H and 3321-2A, Mabtech, Cincinnati, OH). Briefly ...
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bioRxiv - Immunology 2021Quote: ELISpot assays were performed with mouse IFN-γ and IL-4 ELISpot PLUS kits according to the manufacturer’s instructions (3321-4HPT-10 and 3311-4HPW-10, Mabtech, Herndon, VA, USA). A total of 2,5 × 105 or 4 x 105 splenocytes from female mice were seeded per well for the IFN-γ and IL-4 tests respectively and ex vivo stimulated either with the 1:1 mix of the RBD overlapping peptides from the B.1.1.7 (alpha variant ...
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bioRxiv - Immunology 2022Quote: ... Cell suspensions were transferred to pre-coated Human IFN-γ ELISpot plus kit (Mabtech) and developed after 20h according to manufacturer instructions ...
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bioRxiv - Immunology 2024Quote: ... were pre-coated with 15 μg/ml capturing monoclonal anti-human IgG mAbs (Mabtech) and incubated overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: Immunoglobulins (Ig) in culture supernatants were measured using a human IgG ELISA development kit (Mabtech) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... kits with pre-coated plates for enumeration of cells secreting IFN-γ and IL-17A were purchased from Mabtech (Cat. No. FSP-414443-2). Spots were enumerated on an AID iSpot EliSpot/ FluoroSpot Reader.
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bioRxiv - Immunology 2019Quote: ... The ELISA was developed with the use of Goat anti-human IgG biotinylated antibody (Mabtech, Sweden), Streptavidin Alkaline Phosphatase enzyme (Abcam ...
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Dendritic cells focus CTL responses toward highly conserved and topologically important HIV epitopesbioRxiv - Immunology 2020Quote: ... The IFNγ ELISpot assay was performed following the Mabtech Human IFNγ ELISpotBasic protocol (Mabtech, Cincinnati, OH) using 96-well PVDF ELISpot plates from Millipore ...
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bioRxiv - Immunology 2023Quote: IFN-γ ELISpot assays were performed using the Human IFN-γ ELISpot PLUS ALP kit (Mabtech) according to the manufacturer’s protocol with some modifications ...
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bioRxiv - Immunology 2020Quote: T cell responses were assessed by ELISPOT assay using a Human IFNγ ELISPOTPro kit (Mabtech, NS, Sweden) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... ELISpot assay was performed on treated PBMCs using ELISpot Pro: Human IFN-γ (ALP) kit (Mabtech, Sweden), following manufacture’s protocol ...
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bioRxiv - Immunology 2020Quote: ... and immediately washed 6x with 200μL ddH2O and then coated with 100μL/well of PBS + 15μg/mL primary anti-human granzyme B antibody (clone GB10, Mabtech) overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... IFN-γ secreting cells were detected by Human IFN-γ ELISpot Basic Kit (ALP) (Mabtech AB, 3420-2A). The plates were developed using the AP Conjugate Substrate Kit (Biorad ...
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bioRxiv - Immunology 2020Quote: ... On Day 7 of expansion the CD8 population was assessed by Human IFN-γ 96 well ELISPOT (Mabtech) as per manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Cell suspensions were transferred to pre-coated Human IFN-γ ELISpot plus kit plates (Mabtech, Nacka Strand, Sweden) and developed after 20h per manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Antigen-specific IgG was detected by addition of alkaline phosphatase-conjugated anti-human IgG (clone MT78; Mabtech, Cincinnati, OH), followed by p-nitrophenyl phosphate substrate ...
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bioRxiv - Cancer Biology 2023Quote: ... The ELISpot assay was performed on treated PBMCs using the ELISpot Pro: Human IFN-g (ALP) kit (Mabtech, Sweden), following the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: Detection of Perforin and CHI3L1 in iNKT cell culture supernatants was performed using the Human Perforin Elisa Flex (Mabtech) and the Human CHI3L1 DuoSet ELISA (R&D systems ...
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bioRxiv - Immunology 2020Quote: ... were measured using the Human IgG SARS-CoV-2 RBD ELISpotPLUS (ALP) kit according to the manufacturer’s protocol (Mabtech AB).
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bioRxiv - Immunology 2021Quote: ... and IgA production by each sorted B-cell subset were evaluated by using the Human IgG/IgM and IgG/IgA FluoroSpot kits (MABTECH), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... all at 10 µg/ml except for the CEF pool (CEF extended peptide pool for human CD8 T cells, Mabtech) used at 5 µg/ml and cultured overnight at 37°C ...
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Metabolomics reveals nucleoside analogs for regulating mucosal-associated invariant T cell responsesbioRxiv - Immunology 2023Quote: ... The IFNγ+ MAIT cell spots were then developed with an indirect immunostain approach sequentially using a biotinylated anti-human IFNγ antibody (Mabtech), ExtraAvidin conjugated by alkaline phosphatase (Sigma) ...
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bioRxiv - Cancer Biology 2019Quote: ... This method is similar to before(Depla et al., 2008) and were performed using a Human IFN-gamma ELISpot PLUS kit (Mabtech, Sweden) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2021Quote: ... The number of B cells secreting SARS-CoV-2 RBD-specific IgG and total IgG were measured using the Human IgG SARS-CoV-2 RBD ELISpotPLUS kit (Mabtech AB)6.
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bioRxiv - Biochemistry 2020Quote: ... serum samples were centrifuged at 10000 rpm for 20 minutes at 4 °C and supernatants were analyzed by human ApoA-I ELISA (3710-1HP-10, Mabtech Inc.), as well as by denaturant and native western blot (procedure is described in the Supplementary Information file).
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bioRxiv - Molecular Biology 2020Quote: ... Unlabeled freshly recombinantly produced proteins and the mAb 107 of the Human IgE ELISA development kit HRP (Mabtech, Nacka Strand, Sweden), which was used for standardization ...
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bioRxiv - Immunology 2019Quote: The ex vivo IFN-γ ELISpot assay was performed as previously described using a human IFN-γ ELISpot kit (capture mAb -D1K) (Mabtech) 8 ...
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bioRxiv - Immunology 2022Quote: ... and the supernatant collected 24 hours post transfection to measure IFN-α using the Human IFN-α Pan specific ELISA kit (MabTech). MoDCs were lysed using RIPA buffer supplemented with protease inhibitors ...
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bioRxiv - Microbiology 2020Quote: An IFNγ ELISpot assay was used to estimate the frequency and IFNγ production capacity of SARS-CoV-2-specific T cells in PBMCs using a human / simian IFNγ kit (MabTech, Nacka. Sweden), as described previously 46 ...
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bioRxiv - Microbiology 2021Quote: ... pulsed with WT1328-348 (10 µg/ml) followed by measurement of specific cytokine production using the human IFN-γ ELISpot kit (Mabtech AB) and ELIPHOTO Counter (Minerva Tech).
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bioRxiv - Immunology 2020Quote: ... An IFN-γ ELISpot assay was used to estimate the frequency and IFN-γ production capacity of SARS-CoV-2-specific T cells in PBMCs using a human/simian IFN-γ kit (MabTech, Nacka, Sweden). The cells were assayed at 2 x 105 cells per well ...
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bioRxiv - Immunology 2023Quote: TNFα ELISA was performed with the supernatant from T cell and LCL co-culture as described above using the human TNFα ELISABASIC kit (Mabtech, Cat. 3512-1H-6), following manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... followed by 1 h incubation with AP-conjugated streptavidin (1:1000 in PBS, Mabtech). Plates were then washed and visualized using the VECTASTAIN® Elite® ABC-HRP kit according to the manufacturer’s instructions (Mabtech) ...
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bioRxiv - Immunology 2021Quote: ... (mAbs 1-D1K; Mabtech, Stockholm, Sweden) at a concentration of 10ug/mL ...
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bioRxiv - Immunology 2020Quote: ... Streptavidin-HRP (Mabtech – 1/1000 dilution) was added for 30 minutes at room temperature ...
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bioRxiv - Immunology 2022Quote: ... 100 µl of biotinylated secondary anti-IFN-γ antibody (1 µg/ml, clone 7-B6-1, Mabtech) in blocking buffer (PBS 0.5% FBS ...
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bioRxiv - Immunology 2024Quote: ... 100 µl of biotinylated secondary anti-IFN-γ antibody (1 µg/ml, clone 7-B6-1, Mabtech) in blocking buffer (PBS 0.5% FBS ...
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bioRxiv - Immunology 2020Quote: ... and incubated for 1 hour with streptavidin-HRP (diluted 1:100 with D-PBS, Mabtech, 3310-9-1000), and then washed again ...
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bioRxiv - Microbiology 2022Quote: ... Wells were then washed and treated for 1 h at room temperature with 1:1,000 diluted streptavidin-ALP from Mabtech. Afterwards ...
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bioRxiv - Immunology 2022Quote: ... and incubated for 2 h with an IFN-γ detection antibody (1 μg/ml; clone mAb-7B6-1; Mabtech) followed by 1 h incubation with AP-conjugated streptavidin (1:1000 in PBS ...
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bioRxiv - Immunology 2023Quote: ... Wells were then washed and treated for 1 h at room temperature with 1:1,000 diluted streptavidin-ALP from Mabtech. Afterwards ...
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bioRxiv - Immunology 2022Quote: ... anti-IFN-γ 7-B6-1 (biotinylated, Mabtech) as well as streptavidin-horseradish complex was added for visualization.
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bioRxiv - Immunology 2020Quote: ... and incubated for 2 hr at room temperature with biotinylated anti-IFN-γ (clone mAb-7B6-1; 1 μg/ml Mabtech). After six further washes ...
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bioRxiv - Immunology 2021Quote: ... followed by two washes with PBS and then incubated for 2 hr at RT with biotinylated anti-IFN-γ (1 μg/mL; clone mAb-7B6-1; Mabtech). After six further washes ...
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bioRxiv - Immunology 2021Quote: ... followed by streptavidin-alkaline phosphatase (Mabtech, 1:1,000 dilution). Spots were visualised using BCIP/NBT plus substrate and counted via ELISPOT plate reader.
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bioRxiv - Immunology 2021Quote: ... followed by streptavidin-alkaline phosphatase (Mabtech; 1:1,000 dilution). Spots were visualised using BCIP/NBT plus substrate (Mabtech ...