Labshake search
Citations for Mabtech :
151 - 189 of 189 citations for Human Immunodeficiency Virus GP41 Protein HIV 1 Clade B since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2020Quote: ... supplemented with the TLR7 and TLR8 agonist imidazoquinoline resiquimod (R848, 1 µg/ml; Mabtech AB, Nacka, Sweden), and recombinant human IL-2 (10 ng/ml ...
-
bioRxiv - Immunology 2020Quote: ... then washed with PBS-Tween 0.01% before to add anti-mouse IgG antibody (Mabtech – 1/500 dilution). After the last washing ...
-
bioRxiv - Immunology 2022Quote: ... Anti-IFN-γ coating antibody (10 µg/ml in PBS; clone 1-D1K; Mabtech, Nacka Strand, Sweden) was then added and the plates incubated overnight at 4 °C ...
-
bioRxiv - Immunology 2023Quote: ... and incubated with 1 μg/ml of the biotinylated anti-mouse IFNγ monoclonal antibody R4-6A2 (Mabtech) diluted in PBS-0.5% FCS for 2 h ...
-
bioRxiv - Immunology 2020Quote: ... a commercial COVID peptide pool (SARS-CoV-2 S N M O defined peptide pool [3622-1] – Mabtech), and CEF were included as assay controls at a concentration of 1µg/ml ...
-
bioRxiv - Immunology 2020Quote: ... and incubated with 50 μL of biotinylated anti-mouse IFN-γ R4-6A2 mAb (1:1000 dilution; Mabtech) diluted in PBS with 1% FBS for 1 hour at RT ...
-
bioRxiv - Immunology 2023Quote: ... The CD40L-biotin was detected by adding a 1:1,000 dilution of Streptavidin-HRP (Mabtech, 3310-9-1000) and the signal was developed by addition of TMB substrate (BioLegend) ...
-
bioRxiv - Immunology 2023Quote: ... washed and coated overnight with anti-IFN-ψ coating antibody (mAb-1-D1K, Mabtech, Cat. 3420-3-1000). Plates were washed and T cells were co-cultured with autologous LCL in RPMI/ 10% FCS/ 1% Pen/Strep at an effector ...
-
bioRxiv - Bioengineering 2021Quote: ... and wells incubated with 100 µL of 1 µg/ml of the R4-6A2 biotinylated anti-IFN-γ (Mabtech) for 2 hours at r.t ...
-
bioRxiv - Immunology 2020Quote: ... and wells incubated with 100 μL of 1 μg/ml of the R4-6A2 biotinylated anti-IFN-γ (Mabtech) for 2 hours at r.t ...
-
bioRxiv - Immunology 2023Quote: ... The plates were then washed 5 times with PBS and a 1:1000 dilution of ALP-conjugated streptavidin (Mabtech) was added ...
-
bioRxiv - Immunology 2021Quote: ... Plates were then washed and incubated with biotinylated anti-mouse IFN-γ antibody (clone R4-6A2; Mabtech; 1 μg/mL) followed by streptavidin-alkaline phosphatase (Mabtech ...
-
bioRxiv - Immunology 2020Quote: ... Cells were then discarded and plates were further incubated with biotinylated IFNγ antibody (Mab 7-B6-1; Mabtech, Stockholm, Sweden) and incubated for 2hr at 37 C° ...
-
bioRxiv - Immunology 2022Quote: ... Cells were removed and plates were then washed with PBS-0,05% Tween-20 prior incubation with biotinylated anti-IFN-γ antibody (7-B6-1, Mabtech) (2h ...
-
bioRxiv - Immunology 2020Quote: ... Plates were washed 6X with PBS and incubated with 50 µl of streptavidin-alkaline phosphatase conjugate (1:1000 dilution; Mabtech) diluted in PBS 1% FBS ...
-
bioRxiv - Cancer Biology 2021Quote: ... and the wells incubated with 100 μL of 1 μg/ml of the R4-6A2 biotinylated anti-IFN-γ (Mabtech) for 2 hours at r.t ...
-
bioRxiv - Immunology 2022Quote: ... PBMC were thawed and cultured at 37°C and 5% CO2 in the presence of 1 μg/mL R848 and 10 ng/mL of recombinant interleukin (IL) 2 (both from Mabtech).
-
bioRxiv - Immunology 2020Quote: ... cells were removed by washing (using PBS and PBS-Tween 0.02% solution) and 100 µl of biotinylated secondary anti-IFN-γ antibody (clone 7-B6-1, Mabtech) in blocking buffer (PBS 0.5% BSA ...
-
bioRxiv - Immunology 2020Quote: ... ELISpot plates were coated with IFN-γ capture antibody (2 μg/ml per well, Mabtech mAb 1-D1K, 3420-7), in D-PBS and then blocked with RPMI-1640 supplemented media ...
-
bioRxiv - Immunology 2024Quote: ... Plates were washed 6x times with PBST and plated with streptavidin-HRP conjugate (1:1000 dilution; catalogue #3310-9-1000, Mabtech) for 1 hour at room temperature in the dark ...
-
Dendritic cells focus CTL responses toward highly conserved and topologically important HIV epitopesbioRxiv - Immunology 2020Quote: ... T cells were resuspended at a concentration of 3×105 cells per milliliter and 100 μl (3×104 cells/well) were transferred to IFNγ-antibody coated 96-well ELISpot plates (5μg/ml anti-IFNγ mAb 1-D1K, Mabtech, Stockholm, Sweden). Individual 9mer peptide dilutions were done in a separate 1 ml deep-well 96-well plate at a final concentration of 2μg/ml and 100 μl of this dilution was added to T cell-containing wells to give a final peptide concentration of 1μg/ml ...
-
bioRxiv - Immunology 2019Quote: ... was coated overnight at 4 °C with 10 μg ml−1solution of anti-IFN-γ monoclonal antibody (Mabtech clone 1-D1K) in a buffer solution of 0.1 M Na2CO3 ...
-
bioRxiv - Immunology 2020Quote: ... IFN-γ spot forming cells (SFC) were detected by staining membranes with anti-mouse IFN-γ biotin mAb (1 µg/mL; clone R46A2, Mabtech) followed by streptavidin-alkaline phosphatase (1 µg/mL ...
-
bioRxiv - Immunology 2020Quote: ... and a commercial SARS-CoV-2 peptide pool (SARS-CoV-2 S N M O defined peptide pool (3622-1; Mabtech, Sweden) were included as assay controls ...
-
bioRxiv - Microbiology 2023Quote: ... The plates were incubated at 37 °C in 5 % CO2 for 18 h and washed with PBS before the addition of 1 µg/ml biotinylated anti-IFN-γ mAb (Mabtech) at room temperature for 2 h ...
-
bioRxiv - Immunology 2021Quote: ... cells were discarded and FluoroSpot plates were washed and further incubated for 2 hours with cytokine antibodies (mAbs 7-B6-1-BAM; Mabtech, Stockholm, Sweden). Subsequently ...
-
bioRxiv - Immunology 2022Quote: ... ELISpot assays for IFNγ were performed as instructed by the manufacturer using the Mouse IFNγ ELISpot Set (MabTech, cat# Q00824-1×20210623DS). 0.1×106 mouse splenocytes were plated into each well and stimulated overnight with 0.2 µg per peptide per well of RBD-derived overlapping peptides (GenScript).
-
bioRxiv - Immunology 2020Quote: ... cells were discarded and FluoroSpot plates were washed and further incubated for 2hr with cytokine antibodies (mAbs 7-B6-1-BAM, 5A10 biotinylated, MT20D9-WASP, respectively; Mabtech, Stockholm, Sweden). Subsequently ...
-
bioRxiv - Immunology 2020Quote: ... for the enumeration of total IgG-secreting cells with 1 µg per well of anti-porcine IgG (mAb, MT421, Mabtech AB, Sweden) or with culture medium supplemented with 10% FBS (background) ...
-
bioRxiv - Immunology 2021Quote: 96-well FluoroSpot plates were coated with anti-cytokine antibodies for IFNγ and IL-5 (mAbs 1-D1K and TRFK5, respectively; Mabtech, Stockholm, Sweden) at a concentration of 10μg/mL ...
-
bioRxiv - Immunology 2021Quote: ... cells were discarded and plates were washed before the addition of cytokine antibodies (mAbs 7-B6-1-BAM and 5A10-WASP; Mabtech, Stockholm, Sweden). After a 2-hour incubation ...
-
bioRxiv - Immunology 2020Quote: ... and for the enumeration of total IgG-secreting cells with 1 µg per well of anti-porcine IgG (mAb, MT421, Mabtech AB, Sweden) or with culture medium supplemented with 10% FBS (media background control) ...
-
bioRxiv - Immunology 2022Quote: ... were pre-wet with 30% ethanol for 60 seconds and then coated with 100 µl primary anti-IFN-γ antibody solution (10 µg/ml of 1-D1K coating antibody from Mabtech, Cincinnati, OH) OVN at 4°C ...
-
bioRxiv - Immunology 2024Quote: ... were pre-wet with 30% ethanol for 60 seconds and then coated with 100 µl primary anti-IFN-γ antibody solution (10 µg/ml of 1-D1K coating antibody from Mabtech, Cincinnati, OH) OVN at 4°C ...
-
bioRxiv - Immunology 2020Quote: ... The following morning plates were washed with 1x PBS-T before being coated with 3 μg/mL streptavidin-ALP secondary antibody (Mabtech, clone 7-B6-1) and incubated for 2 hours at room temperature ...
-
bioRxiv - Immunology 2021Quote: ... washed with sterile PBS and coated overnight at 4 °C with anti–IFN-γ antibody (10 µg/ml in PBS; clone 1-D1K; Mabtech, Nacka Strand, Sweden). Prior to use ...
-
bioRxiv - Immunology 2022Quote: Single cell suspensions from lungs and spleens of mice were stimulated with mouse IL-2 (R & D Systems, Cat # 402-ML; 0.5 μg/mL) and RD848 (Mabtech, Cat # 3611-5X; 1 μg/mL) for 3 days to induce nonspecific polyclonal expansion ...
-
bioRxiv - Immunology 2023Quote: ... Wells were washed and incubated for 2 hours with biotinylated anti-IFN-ψ detection antibody (mAb-7-B6-1-Biotin, Mabtech, Cat. 3420-6-250). Afterwards ...
-
bioRxiv - Immunology 2020Quote: ... were pre-wet with 70% methanol and then coated with 100 µl primary anti-IFN-γ antibody solution (10 µg/ml of 1-D1K coating antibody from Mabtech in PBS, pH 7.4, V-E4) OVN at 4°C ...