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Citations for Mabtech :
201 - 250 of 270 citations for Human Angiopoietin 1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... The stimulated splenocytes were analyzed for IFN-γ responses using the mouse IFN-γ ELISpot Plus kit (Mabtech AB, Sweden). Spot-forming cells (SFU ...
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bioRxiv - Immunology 2019Quote: ... NS1-specific IFNγ and IL-5 production by ZIKV-specific splenic T cells were enumerated by Enzyme Linked ImmunoSpot (ELISPOT) assay following manufacturer’s instructions (Mouse IFN-γ/IL-5 ELISpot Basic kits; Mabtech). Mouse splenocytes were left unstimulated or stimulated with an NS1 peptide pool (PepMix ZIKV NS1 Ultra ...
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bioRxiv - Immunology 2020Quote: ... IgG- and IgM-ASCs in freshly isolated PBMCs were measured using a multicolor B cell FluoroSpot kit with modifications (Mabtech). Briefly ...
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bioRxiv - Immunology 2020Quote: IFN-γ ELISpot was performed on splenocytes of mice isolated after sacrifice using mouse IFN-γ ELISpot-plus kit (Mabtech). Splenocytes were obtained by disaggregation of spleens with the gentleMACS dissociator ...
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Efficacy of recombinant NP-M1 and NP-M1-CRT DNA vaccines against Influenza A viruses in mice C57/BL6bioRxiv - Molecular Biology 2021Quote: ... The number of NP-specific IFN□ secreting cells in mouse was counted using commercial ELISPOT assay kits (MABTECH AB, Sweden). This procedure was conducted according to the instruction manual ...
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bioRxiv - Immunology 2022Quote: ... and cellular responses were determined on fresh PBMCs at Charles River Laboratories using a monkey IFN-γ ELISpot kit (Mabtech) at Weeks 1 ...
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bioRxiv - Immunology 2023Quote: ... plates were developed using reagents and following procedures and incubation periods described in the pig IFNγ ELISpot Plus kit (3130-4HPW-10, Mabtech). Spots were counted under a dissection microscope (Leica GZ6) ...
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bioRxiv - Microbiology 2024Quote: ... Interferon-gamma (IFN-γ) and interleukin-4 (IL-4) were quantified with commercially-available kits (MABTECH AB and FineTest, respectively) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... and coated overnight at 4°C with anti-IFN-γ antibody (1-DIK, Mabtech). Plates were washed using PBS and then saturated with RPMI complemented with 10% FBS ...
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bioRxiv - Cancer Biology 2022Quote: ... development was performed with an IFN-γ- detection antibody (7-B6-1-biotin, Mabtech) and Streptavidin-HRP (Mabtech) ...
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bioRxiv - Immunology 2023Quote: ... Cells were then incubated with IFNγ mAb (7-B6-1-BAM Mabtech, Stockholm, Sweden) for 2 hours and developed.
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bioRxiv - Immunology 2022Quote: The T cell immune responses in rhesus macaques were detected using the PBMCs with commercially available NHP IFN-γ and IL-2 ELISpot assay kits (Mabtech), and NHP IL-4 ELISpot assay kit (U-CyTech) ...
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bioRxiv - Immunology 2021Quote: Antigen-specific circulating PBMCs were evaluated using pre-coated FluoroSpot plates and kits purchased from Mabtech (Mabtech AB, Nacka Strand, Sweden) and used according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: Total splenocytes were cultured on anti-IgG ELISpot plates and developed using the standardized ELISpot kit (Mabtech Inc., Cincinnati, OH, USA). Briefly ...
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bioRxiv - Immunology 2020Quote: IFNy ELISpot assay of PBMCs was performed using the Monkey IFNy ELISpot PRO kit (Mabtech Monkey IFNy ELISPOT pro, #3421M-2APT) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Plates were then washed and visualized using the VECTASTAIN® Elite® ABC-HRP kit according to the manufacturer’s instructions (Mabtech). Antigen-specific T cell responses were quantified by subtracting the number of spots in unstimulated cells from the peptide stimulated cells ...
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bioRxiv - Microbiology 2020Quote: Murine IFN-γ and IL-5 ELISPOT assays were performed following manufacturer’s procedures for mouse IFN-γ and IL-5 ELISPOT kit (Mabtech, Cincinnati, OH). Briefly ...
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bioRxiv - Microbiology 2019Quote: Freshly isolated PBMCs (2-2.5 × 105/well) were stimulated for 16-18 hours in a 96-well pre-coated plate (ELISpotPLUS kit, MABTECH Inc., Mariemont, OH) at 37°C in a 5% CO2 incubator ...
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bioRxiv - Immunology 2021Quote: Murine IFN-γ and IL-5 ELISpot assays were performed following the manufacturer’s procedures for mouse IFN-γ and IL-5 ELISpot kits (3321-2H and 3321-2A, Mabtech, Cincinnati, OH). Briefly ...
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bioRxiv - Immunology 2021Quote: ELISpot assays were performed with mouse IFN-γ and IL-4 ELISpot PLUS kits according to the manufacturer’s instructions (3321-4HPT-10 and 3311-4HPW-10, Mabtech, Herndon, VA, USA). A total of 2,5 × 105 or 4 x 105 splenocytes from female mice were seeded per well for the IFN-γ and IL-4 tests respectively and ex vivo stimulated either with the 1:1 mix of the RBD overlapping peptides from the B.1.1.7 (alpha variant ...
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bioRxiv - Immunology 2022Quote: Spleens from immunized animals were removed at Day 51 for the mice and at Day 110 for the hamsters and splenocytes were isolated for protein S-specific T-cell detection with the ELISpotPLUS mouse IFN-γ or ELISpotPLUS hamster IFN-γ kits (Mabtech) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... and the plates were incubated with the detection antibody 7-B6-1-biotin (Mabtech, Sweden) diluted 1:1000 in PBS + 0.5% FCS and incubated for two hours at room temperature ...
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bioRxiv - Microbiology 2023Quote: Interferon (IFN)-γ Enzyme-linked ImmunoSpot (ELISPOT) assay was performed as described previously160 using the Mouse IFN-γ ELISpot kit (Mabtech, Stockholm, Sweden) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... and ELISPOT plates were developed following the manufacturer’s protocol for the ELISpot Plus: Mouse IFN-γ (ALP) kit (Mabtech Cat. No. 3321-4APW). After the plates were developed ...
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bioRxiv - Immunology 2023Quote: ... Washed plates were incubated with alkaline-phosphatase-conjugated mAb for IFN-γ (Mabtech #7-B6-1) and with biotinylated mAb for IL-2 (Mabtech #MT8G10) ...
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bioRxiv - Immunology 2021Quote: Hamster interferon gamma (IFN-γ) enzyme-linked immunosorbent spot (ELISpot) analysis was performed using the Hamster IFN-γ ELISpotBASIC kit (MABTECH, Nacka Strand, Sweden) in combination with multiscreen immunoprecipitation (IP ...
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bioRxiv - Immunology 2022Quote: ... kits with pre-coated plates for enumeration of cells secreting IFN-γ and IL-17A were purchased from Mabtech (Cat. No. FSP-414443-2). Spots were enumerated on an AID iSpot EliSpot/ FluoroSpot Reader.
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bioRxiv - Microbiology 2024Quote: ... The diluted plasma was determined to determine IFN-γ levels using a commercial monkey IFN-γ ELISApro kit (catalog no. M4210M-1HP-10, Mabtech AB, Sweden), containing mAb MT126L ...
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bioRxiv - Immunology 2021Quote: ... supplemented with the TLR7 and TLR8 agonist imidazoquinoline resiquimod (R848, 1 μg/ml; Mabtech AB, Nacka, Sweden), and recombinant human IL-2 (10 ng/ml ...
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bioRxiv - Immunology 2020Quote: ... and incubated with 50 μL of biotinylated anti-porcine IFN-γ mAb P2C11 (1:1000 dilution; Mabtech) after overnight incubation ...
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bioRxiv - Immunology 2020Quote: ... supplemented with the TLR7 and TLR8 agonist imidazoquinoline resiquimod (R848, 1 µg/ml; Mabtech AB, Nacka, Sweden), and recombinant human IL-2 (10 ng/ml ...
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bioRxiv - Immunology 2020Quote: ... then washed with PBS-Tween 0.01% before to add anti-mouse IgG antibody (Mabtech – 1/500 dilution). After the last washing ...
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bioRxiv - Immunology 2022Quote: ... Anti-IFN-γ coating antibody (10 µg/ml in PBS; clone 1-D1K; Mabtech, Nacka Strand, Sweden) was then added and the plates incubated overnight at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... and incubated with 1 μg/ml of the biotinylated anti-mouse IFNγ monoclonal antibody R4-6A2 (Mabtech) diluted in PBS-0.5% FCS for 2 h ...
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bioRxiv - Immunology 2020Quote: ... a commercial COVID peptide pool (SARS-CoV-2 S N M O defined peptide pool [3622-1] – Mabtech), and CEF were included as assay controls at a concentration of 1µg/ml ...
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bioRxiv - Immunology 2020Quote: ... and incubated with 50 μL of biotinylated anti-mouse IFN-γ R4-6A2 mAb (1:1000 dilution; Mabtech) diluted in PBS with 1% FBS for 1 hour at RT ...
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bioRxiv - Immunology 2023Quote: ... The CD40L-biotin was detected by adding a 1:1,000 dilution of Streptavidin-HRP (Mabtech, 3310-9-1000) and the signal was developed by addition of TMB substrate (BioLegend) ...
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bioRxiv - Immunology 2023Quote: ... washed and coated overnight with anti-IFN-ψ coating antibody (mAb-1-D1K, Mabtech, Cat. 3420-3-1000). Plates were washed and T cells were co-cultured with autologous LCL in RPMI/ 10% FCS/ 1% Pen/Strep at an effector ...
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bioRxiv - Bioengineering 2021Quote: ... and wells incubated with 100 µL of 1 µg/ml of the R4-6A2 biotinylated anti-IFN-γ (Mabtech) for 2 hours at r.t ...
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bioRxiv - Immunology 2020Quote: ... and wells incubated with 100 μL of 1 μg/ml of the R4-6A2 biotinylated anti-IFN-γ (Mabtech) for 2 hours at r.t ...
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bioRxiv - Immunology 2023Quote: ... The plates were then washed 5 times with PBS and a 1:1000 dilution of ALP-conjugated streptavidin (Mabtech) was added ...
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bioRxiv - Immunology 2021Quote: ... Plates were then washed and incubated with biotinylated anti-mouse IFN-γ antibody (clone R4-6A2; Mabtech; 1 μg/mL) followed by streptavidin-alkaline phosphatase (Mabtech ...
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bioRxiv - Immunology 2020Quote: ... Cells were then discarded and plates were further incubated with biotinylated IFNγ antibody (Mab 7-B6-1; Mabtech, Stockholm, Sweden) and incubated for 2hr at 37 C° ...
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bioRxiv - Immunology 2022Quote: ... Cells were removed and plates were then washed with PBS-0,05% Tween-20 prior incubation with biotinylated anti-IFN-γ antibody (7-B6-1, Mabtech) (2h ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed 6X with PBS and incubated with 50 µl of streptavidin-alkaline phosphatase conjugate (1:1000 dilution; Mabtech) diluted in PBS 1% FBS ...
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bioRxiv - Cancer Biology 2021Quote: ... and the wells incubated with 100 μL of 1 μg/ml of the R4-6A2 biotinylated anti-IFN-γ (Mabtech) for 2 hours at r.t ...
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bioRxiv - Immunology 2022Quote: ... PBMC were thawed and cultured at 37°C and 5% CO2 in the presence of 1 μg/mL R848 and 10 ng/mL of recombinant interleukin (IL) 2 (both from Mabtech).
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bioRxiv - Immunology 2020Quote: ... cells were removed by washing (using PBS and PBS-Tween 0.02% solution) and 100 µl of biotinylated secondary anti-IFN-γ antibody (clone 7-B6-1, Mabtech) in blocking buffer (PBS 0.5% BSA ...
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bioRxiv - Immunology 2020Quote: ... ELISpot plates were coated with IFN-γ capture antibody (2 μg/ml per well, Mabtech mAb 1-D1K, 3420-7), in D-PBS and then blocked with RPMI-1640 supplemented media ...
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bioRxiv - Immunology 2024Quote: ... Plates were washed 6x times with PBST and plated with streptavidin-HRP conjugate (1:1000 dilution; catalogue #3310-9-1000, Mabtech) for 1 hour at room temperature in the dark ...