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Citations for Mabtech :
51 - 93 of 93 citations for Fc gamma RIIIA Human F176V HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... were either directly coated with 100μL/well of PBS + 0.5μg/mL primary anti-human IFN-γ antibody (clone 1-D1K, Mabtech) overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... IFN-γ secreting cells were detected by Human IFN-γ ELISpot Basic Kit (ALP) (Mabtech AB, 3420-2A). The plates were developed using the AP Conjugate Substrate Kit (Biorad ...
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bioRxiv - Immunology 2020Quote: ELISpot plates were coated with 10 μg/mL anti-human IFN-γ coating antibody (Mabtech, clone 1-D1K) and incubated overnight at 4C° ...
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bioRxiv - Immunology 2020Quote: ... On Day 7 of expansion the CD8 population was assessed by Human IFN-γ 96 well ELISPOT (Mabtech) as per manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Cell suspensions were transferred to pre-coated Human IFN-γ ELISpot plus kit plates (Mabtech, Nacka Strand, Sweden) and developed after 20h per manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... FluoroSpot assays were performed according to the manufacturer’s recommendations (FluoroSpot Plus: Human IFNγ/IL-2/TNFα kit, MABTech). Briefly ...
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bioRxiv - Immunology 2019Quote: ... then added with alkaline phosphatase (ALP) labeled anti-human IFN-γ mAb (7-B6-1-ALP, 1:200; Mabtech) for 2 hours ...
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bioRxiv - Immunology 2019Quote: IFN-γ ELISPOT assay strip plate which was pre-coated with anti-human IFN-γ mAb (1-D1K, Mabtech) was washed with PBS and blocked with RPMI-1640 containing 10% FBS ...
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bioRxiv - Immunology 2020Quote: ... Antigen-specific IgG was detected by addition of alkaline phosphatase-conjugated anti-human IgG (clone MT78; Mabtech, Cincinnati, OH), followed by p-nitrophenyl phosphate substrate ...
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bioRxiv - Cancer Biology 2023Quote: ... The ELISpot assay was performed on treated PBMCs using the ELISpot Pro: Human IFN-g (ALP) kit (Mabtech, Sweden), following the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: Detection of Perforin and CHI3L1 in iNKT cell culture supernatants was performed using the Human Perforin Elisa Flex (Mabtech) and the Human CHI3L1 DuoSet ELISA (R&D systems ...
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bioRxiv - Immunology 2020Quote: ... were measured using the Human IgG SARS-CoV-2 RBD ELISpotPLUS (ALP) kit according to the manufacturer’s protocol (Mabtech AB).
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bioRxiv - Immunology 2021Quote: ... and IgA production by each sorted B-cell subset were evaluated by using the Human IgG/IgM and IgG/IgA FluoroSpot kits (MABTECH), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... anti-human IgA-490 (green fluorescence) and anti-human IgM-640 (red fluorescence) secondary detection antibodies (diluted 1:500 each) (all antibodies from Mabtech). Fluorescent spots indicating a single ASC were detected with an IRIS FluoroSpot reader and counted with Apex software (Mabtech).
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bioRxiv - Immunology 2020Quote: IL-6 levels in serum from patients and healthy controls were measured in freshly thawed serum using human IL-6 ELISA development kit (Mabtech), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... the plates were developed with human biotinylated IFN-γ detection antibody (Clone 7-B6-1, 1 μg/ml, Mabtech, Sweden), followed by incubation with Streptavidin-AP (1:1000 ...
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bioRxiv - Microbiology 2022Quote: ... all at 10 µg/ml except for the CEF pool (CEF extended peptide pool for human CD8 T cells, Mabtech) used at 5 µg/ml and cultured overnight at 37°C ...
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Metabolomics reveals nucleoside analogs for regulating mucosal-associated invariant T cell responsesbioRxiv - Immunology 2023Quote: ... The IFNγ+ MAIT cell spots were then developed with an indirect immunostain approach sequentially using a biotinylated anti-human IFNγ antibody (Mabtech), ExtraAvidin conjugated by alkaline phosphatase (Sigma) ...
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bioRxiv - Immunology 2021Quote: ... The SARS-CoV-2 S1 scanning pool contains 166 peptides from the human SARS-CoV-2 virus (#3629-1, Mabtech AB). The peptides are 15-mers overlapping with 11 amino acids ...
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bioRxiv - Immunology 2021Quote: ... The SARS-CoV-2 S2 N defined peptide pool contains 41 synthetic peptides binding to human HLA derived from the S and N proteins of the SARS-CoV-2 virus (#3620-1, Mabtech AB)58.
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bioRxiv - Immunology 2021Quote: ... The number of B cells secreting SARS-CoV-2 RBD-specific IgG and total IgG were measured using the Human IgG SARS-CoV-2 RBD ELISpotPLUS kit (Mabtech AB)6.
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bioRxiv - Immunology 2020Quote: ... The SARS-CoV-2 S2 N defined peptide pool contains 41 synthetic peptides binding to human HLA derived from the S and N proteins of the SARS-CoV-2 virus (#3620-1, Mabtech AB) 20 ...
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bioRxiv - Immunology 2020Quote: The SARS-CoV-2 S1 scanning pool contains 166 peptides from the human SARS-CoV-2 virus (#3629-1, Mabtech AB). The peptides are 15-mers overlapping with 11 amino acids ...
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bioRxiv - Immunology 2020Quote: IFN-γ and IL-2 secreting T cells were detected using Human IFN-γ/IL-2 SARS-CoV-2 FluoroSpotPLUS kits according to the manufacture’s protocol (Mabtech AB). The plates pre-coated with capturing monoclonal anti-IFN-γ and anti-IL-2 were incubated overnight in RPMI-1640 medium containing 10% FCS supplemented with a mixture containing the SARS-CoV-2 peptide pool (scanning or defined pools) ...
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bioRxiv - Microbiology 2021Quote: ... at an E:T ratio of 5:1 for 24 hours followed by measurement of specific cytokine production using the human IL-2 ELISpot kit (Mabtech AB) and ELIPHOTO Counter (Minerva Tech).
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bioRxiv - Biochemistry 2020Quote: ... serum samples were centrifuged at 10000 rpm for 20 minutes at 4 °C and supernatants were analyzed by human ApoA-I ELISA (3710-1HP-10, Mabtech Inc.), as well as by denaturant and native western blot (procedure is described in the Supplementary Information file).
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bioRxiv - Immunology 2021Quote: To assess memory T cell responses to SARS-CoV-2 in convalescent COVID-19 patient samples we utilized Human IFN-γ/IL-2/TNF FluoroSpot kit (Mabtech), which allows for the detection of T cells producing IFN-γ ...
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bioRxiv - Molecular Biology 2020Quote: ... Unlabeled freshly recombinantly produced proteins and the mAb 107 of the Human IgE ELISA development kit HRP (Mabtech, Nacka Strand, Sweden), which was used for standardization ...
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bioRxiv - Immunology 2022Quote: ... followed by 2 hours of incubation at 37°C with biotinylated anti-human IFN-γ monoclonal antibodies (clone 7-B6-1, Mabtech) diluted at 1μg/ml in PBS ...
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bioRxiv - Bioengineering 2023Quote: ... were collected for ELISpot assays using ELISpot Plus: Monkey IFN-γ (HRP) and ELISpot Plus: Human IL-4 (HRP) kits (Mabtech). 2 × 105 PBMCs seeded onto 96 well plates in the kits were incubated with 1 μg of PepMixTM SARS-CoV-2 (Spike Glycoprotein ...
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bioRxiv - Immunology 2022Quote: Incubate the array with 10 μg/mL biotinylated anti-human IFNγ 7-B6-1 (MABTECH AB, Stockholm, Sweden, Cat #3420-6-250) and then 12 μg/mL AF647-Streptavidin (Jackson ImmunoResearch ...
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bioRxiv - Immunology 2021Quote: ... splenocytes or lung leukocytes were stimulated with 1 μg/ml R848 and 10 ng/ml recombinant human IL-2 (Mabtech In, OH). Millipore ELISPOT plates (Millipore Ltd ...
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bioRxiv - Immunology 2020Quote: ... 5% CO2 at a concentration of 1×105 cells/mL media on plates previously coated with anti-human IFNγ (Mab 1-D1K; Mabtech, Stockholm, Sweden). Cells were then discarded and plates were further incubated with biotinylated IFNγ antibody (Mab 7-B6-1 ...
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bioRxiv - Immunology 2019Quote: The ex vivo IFN-γ ELISpot assay was performed as previously described using a human IFN-γ ELISpot kit (capture mAb -D1K) (Mabtech) 8 ...
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bioRxiv - Immunology 2021Quote: S1 and S N M O specific IFN-γ and IL-2-secreting T cells were detected using the Human IFN-γ/IL-2 SARS-CoV-2 FluoroSpotPLUS kit (Mabtech AB)6 ...
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bioRxiv - Microbiology 2020Quote: ... Baboon IFN-γ and IL-4 assays were carried out using NHP IFN-γ and Human IL-4 assay kit from Mabtech using PBMC collected at day 7 following the second immunization (day 28).
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bioRxiv - Immunology 2022Quote: ... and the supernatant collected 24 hours post transfection to measure IFN-α using the Human IFN-α Pan specific ELISA kit (MabTech). MoDCs were lysed using RIPA buffer supplemented with protease inhibitors ...
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bioRxiv - Microbiology 2020Quote: An IFNγ ELISpot assay was used to estimate the frequency and IFNγ production capacity of SARS-CoV-2-specific T cells in PBMCs using a human / simian IFNγ kit (MabTech, Nacka. Sweden), as described previously 46 ...
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bioRxiv - Microbiology 2021Quote: ... pulsed with WT1328-348 (10 µg/ml) followed by measurement of specific cytokine production using the human IFN-γ ELISpot kit (Mabtech AB) and ELIPHOTO Counter (Minerva Tech).
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bioRxiv - Immunology 2021Quote: ... baboon IFN-γ and IL-4 assays were carried out using NHP IFN-γ and Human IL-4 assay kits from Mabtech. Assays were performed in triplicate.
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bioRxiv - Immunology 2020Quote: ... Controls included 1×105 effector CD8+ T cells co-cultured with anti-human CD3 monoclonal antibody (0.5 µg/mL, Mabtech, 3605-1-1000), or with media alone ...
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bioRxiv - Immunology 2020Quote: ... An IFN-γ ELISpot assay was used to estimate the frequency and IFN-γ production capacity of SARS-CoV-2-specific T cells in PBMCs using a human/simian IFN-γ kit (MabTech, Nacka, Sweden). The cells were assayed at 2 x 105 cells per well ...
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bioRxiv - Immunology 2023Quote: TNFα ELISA was performed with the supernatant from T cell and LCL co-culture as described above using the human TNFα ELISABASIC kit (Mabtech, Cat. 3512-1H-6), following manufacturer’s instructions ...