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Citations for Mabtech :
101 - 150 of 198 citations for Cow Probable fructose 2 6 bisphosphatase TIGAR TIGAR ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... and 2 x 105 splenocytes were plated into each well of the Mouse IFN-γ ELISpotPLUS plates (MabTech). The splenocytes were stimulated for 20 hours at 37°C with RBD peptides (15-mer peptides overlapping by 9 amino acid spanning the RBD of SARS-CoV-2 spike protein ...
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bioRxiv - Immunology 2021Quote: ... 2×105 cells were added to each well of pre-coated Immobilon-FL PVDF 96-well plates (Mabtech) in the presence of 2 μg/ml peptide pool ...
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bioRxiv - Immunology 2020Quote: ... a commercial COVID peptide pool (SARS-CoV-2 S N M O defined peptide pool [3622-1] – Mabtech), and CEF were included as assay controls at a concentration of 1µg/ml ...
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bioRxiv - Immunology 2023Quote: ... 2×103 transfected T cells were plated per well in a pre-coated 96-well ELISpot plate (Mabtech) together with 5×104 PHA-activated autologous CD4 T cells as antigen-presenting cells and challenged with the specific peptide ...
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bioRxiv - Immunology 2021Quote: ... IL-2 (Cy3) and/or TNF (Cy5) were detected with IRIS FluoroSpot reader and counted with Apex software (Mabtech). Numbers of responding cells in negative controls were subtracted from stimulated samples to account for background responses ...
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bioRxiv - Immunology 2021Quote: ... cryopreserved PBMCs from study subjects were stimulated for 5 days with polyclonal B cell stimulation (1μg/mL R848 and 10 ng/mL rIL-2, Mabtech) in R10 media (RPMI 1640 with 10% FCS ...
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bioRxiv - Immunology 2022Quote: ... and incubated for 2 h with an IFN-γ detection antibody (1 μg/ml; clone mAb-7B6-1; Mabtech) followed by 1 h incubation with AP-conjugated streptavidin (1:1000 in PBS ...
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bioRxiv - Microbiology 2019Quote: ... an enzyme-linked immunosorbent assay kit detecting mouse IgG (Mabtech) was used ...
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bioRxiv - Immunology 2020Quote: ... the Monkey IFN-γ ELISpotPLUS kit (MABTECH 3421M-APT-10) was used as directed by the manufacturer ...
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bioRxiv - Immunology 2022Quote: A human IFNγ ELISpotplus kit (MABTECH AB, Nacka Strand, Sweden) was used in accordance with the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... and Monkey IL17A ELISpotPLUS Kit (Mabtech, Cat #3520M-4APW-10) were used following manufacturer’s instructions with 2×105 freshly isolated PBMC added to each well ...
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bioRxiv - Immunology 2023Quote: ... Ninety six-well mouse IFN-γ ELISPOT kit (Mabtech, USA) plates pre-coated with anti-mouse IFN-γ capture antibody were rinsed 5 times with PBS to hydrate the membrane and then blocked with supplemented CTL media for at least 30 minutes ...
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bioRxiv - Microbiology 2022Quote: ... and the plate was incubated for 2 h at room temperature with R4-6A2 biotinylated anti-IFN-γ antibody (Mabtech) at the concentration of 100 μg/mL ...
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bioRxiv - Pathology 2022Quote: ... The cells were incubated for 20 hours at 37°C in 5% CO2 incubator in pre-coated Ferret IFNγ-ELISpot plates (3112-4APW-2, Mabtech). The assay was performed following the manufacturers protocol ...
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bioRxiv - Immunology 2022Quote: ... PBMC were thawed and cultured at 37°C and 5% CO2 in the presence of 1 μg/mL R848 and 10 ng/mL of recombinant interleukin (IL) 2 (both from Mabtech).
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bioRxiv - Immunology 2020Quote: ... ELISpot plates were coated with IFN-γ capture antibody (2 μg/ml per well, Mabtech mAb 1-D1K, 3420-7), in D-PBS and then blocked with RPMI-1640 supplemented media ...
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bioRxiv - Immunology 2022Quote: ... Splenocytes were counted and 250,000 cells were plated per well into pre-covered plates and stimulated overnight with 10 μg/mL SARS-CoV-2 (S1) peptide pool (Mabtech) or medium as negative control or PHA 2 μg/mL as positive experimental control ...
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bioRxiv - Immunology 2023Quote: ... and the plate was incubated for 2 h at room temperature with R4-6A2 biotinylated anti-IFN-γ antibody (Mabtech) at the concentration of 100 μg/mL ...
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bioRxiv - Immunology 2023Quote: ... the 2CZ T cell hybridoma was used and the IL-2 secreted upon its activation was quantified using an ELISPOT assay (Mabtech) as previously described 7.
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bioRxiv - Immunology 2023Quote: ... Monkey IFNγ ELISpot PRO kit (Mabtech, Cat #3421M-2APT, Nacka, Sweden) and Monkey IL17A ELISpotPLUS Kit (Mabtech ...
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bioRxiv - Immunology 2023Quote: IFNγ ELISpot assays were performed using a commercially available kit (Mabtech). 2.5 x 105 CD4+ T cells were incubated with 2.5 x 105 irradiated APCs in 200 μl R10 media (RPMI 1640 + 10% FBS ...
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bioRxiv - Immunology 2021Quote: ... The SARS-CoV-2 S2 N defined peptide pool contains 41 synthetic peptides binding to human HLA derived from the S and N proteins of the SARS-CoV-2 virus (#3620-1, Mabtech AB)58.
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bioRxiv - Immunology 2020Quote: ... and incubated for 2 hr at room temperature with biotinylated anti-IFN-γ (clone mAb-7B6-1; 1 μg/ml Mabtech). After six further washes ...
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bioRxiv - Immunology 2020Quote: ... The SARS-CoV-2 S2 N defined peptide pool contains 41 synthetic peptides binding to human HLA derived from the S and N proteins of the SARS-CoV-2 virus (#3620-1, Mabtech AB) 20 ...
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bioRxiv - Immunology 2021Quote: ... followed by two washes with PBS and then incubated for 2 hr at RT with biotinylated anti-IFN-γ (1 μg/mL; clone mAb-7B6-1; Mabtech). After six further washes ...
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bioRxiv - Immunology 2022Quote: ... followed by 2 hours of incubation at 37°C with biotinylated anti-human IFN-γ monoclonal antibodies (clone 7-B6-1, Mabtech) diluted at 1μg/ml in PBS ...
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bioRxiv - Immunology 2022Quote: ELISpot experiment was performed with Human IgM ELISpotBASIC kit (ALP) from Mabtech AB ...
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bioRxiv - Immunology 2022Quote: ... we used antigen-specific IFN-γ ELISpot kits (MABTECH, 3321-4HPT-10) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... and PN-specific IgG ELISpots were performed with commercially available kits (Mabtech), as per manufacturer’s recommendations.
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bioRxiv - Immunology 2021Quote: ... were measured using a multicolor B cell FluoroSpot kit with modifications (Mabtech). Briefly ...
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bioRxiv - Immunology 2021Quote: ... splenocytes or lung leukocytes were stimulated with 1 μg/ml R848 and 10 ng/ml recombinant human IL-2 (Mabtech In, OH). Millipore ELISPOT plates (Millipore Ltd ...
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bioRxiv - Immunology 2021Quote: ... cells were discarded and FluoroSpot plates were washed and further incubated for 2 hours with cytokine antibodies (mAbs 7-B6-1-BAM; Mabtech, Stockholm, Sweden). Subsequently ...
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bioRxiv - Cancer Biology 2021Quote: ... Plates were developed by washing 6 times in PBS/0.05%Tween 20 followed by incubation with biotinylated IFN-γ detection antibody (0.5 mg/mL; Mabtech; 2 hours, 37°C). This was followed by a further 6 washes in PBS/0.05%Tween 20 and incubation with avidin DH-coupled biotinylated peroxidase H complex (Vectastain Elite ABC Kit ...
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bioRxiv - Immunology 2020Quote: ... Cellular immune responses were measured by IFN-γ FluoroSpot assay according to the manufacturer’s instructions (Cat. No. FSP-4246-2 Mabtech, Inc., Cincinnati, OH). Briefly ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 antigen RBD01-dn5B was diluted to 80 mg/mL and used to coat wells of PVDF plates (Mabtech, #3654-WP-10) according to the assay kit protocol ...
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bioRxiv - Immunology 2022Quote: ... ELISpot assays were performed using Monkey IFNγ ELISpotPLUS kits (MabTech, cat# 3241M-4HPW). Precoated 96-well ELISpot plates were blocked with RPMI + 10% FBS for 2 hours at room temperature ...
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bioRxiv - Microbiology 2019Quote: ... Antigen-specific IgG ELISpot assays were performed using Human IgG ELISpotBASIC kit (MabTech). PBMCs were first pre-stimulated with a mixture of R848 at 1 μg/ml and rhIL-2 at 10 ng/ml for 96h ...
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bioRxiv - Immunology 2023Quote: T cell responses were measured using a IFN-γ ELISpot Pro kit (Mabtech) as previously described ...
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bioRxiv - Immunology 2022Quote: ... Cell suspensions were transferred to pre-coated Human IFN-γ ELISpot plus kit (Mabtech) and developed after 20h according to manufacturer instructions ...
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bioRxiv - Immunology 2022Quote: Mouse IFN-γ and IL-4 ELISpot assays were performed with ELISpot kits (Mabtech) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... IFN-γ and IL-17A ELISPOT assays were performed using Human ELISpotplus kit (MabTech) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... ELISpot was performed using mouse IFN-γ ELISpot PLUS kits (MABTECH, Cincinnati, OH, USA) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: Single cell suspensions from lungs and spleens of mice were stimulated with mouse IL-2 (R & D Systems, Cat # 402-ML; 0.5 μg/mL) and RD848 (Mabtech, Cat # 3611-5X; 1 μg/mL) for 3 days to induce nonspecific polyclonal expansion ...
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bioRxiv - Immunology 2020Quote: ELISpot assays were performed with mouse IFNγ ELISpotPLUS kits according to the manufacturer’s instructions (Mabtech). A total of 5 × 105 splenocytes was ex vivo restimulated with the full-length S peptide mix (0.1 µg/mL final concentration per peptide ...
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bioRxiv - Microbiology 2021Quote: ... cells were cultured for 24 h and detected using a commercial kit (MABTECH, Nacka, Sweden) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... IFNγ ELISPOT was performed using the Mouse IFNγ ELISPOTPLUS kit (MABTECH product # 3321-4HPW-10). ELISPOT plates were analyzed using ImmunoSpot CTL plate reader (CTL ImmunoSpot S6 Entry Analyzer ...
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bioRxiv - Immunology 2022Quote: ... Cells were plated in the Mouse IFN-γ ELISPOTPLUS kit (ALP) (MABTECH, Nacka Strand, Sweden) and IFN-γ spots were detected after 48 h using ELISPOT reader systems (Autoimmun Diagnostika GmbH ...
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bioRxiv - Immunology 2024Quote: ... IFNγ and TNFα ELISpot were performed using monkey cytokine specific ELISpotPLUS (ALP) KITs (Mabtech, Inc.) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... ELISpot assays were performed using the Rat IFN-γ ELISpotPLUS kit (MABTECH, Cat. No.: 3220- 4APW), Rat IL-4 T cell ELISpot kit (U-CyTech ...
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bioRxiv - Immunology 2020Quote: Murine ELISpot assays were performed with mouse IFNγ ELISpotPLUS kits according to the manufacturer’s instructions (Mabtech). A total of 5 × 105 splenocytes was ex vivo restimulated with the full-length S peptide mix (0.1 μg/mL final concentration per peptide ...