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Citations for Mabtech :
51 - 100 of 166 citations for 8 Chloro 2 chloromethyl imidazo 1 2 a pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... 2×103 transfected T cells were plated per well in a pre-coated 96-well ELISpot plate (Mabtech) together with 5×104 PHA-activated autologous CD4 T cells as antigen-presenting cells and challenged with the specific peptide ...
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bioRxiv - Immunology 2023Quote: ... FluoroSpot assays were performed according to the manufacturer’s recommendations (FluoroSpot Plus: Human IFNγ/IL-2/TNFα kit, MABTech). Briefly ...
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bioRxiv - Microbiology 2022Quote: IL-4 and IL-2 ELISPOT assays were performed with mouse IL-4 ELISPOTPLUS kits and mouse IL-2 ELISPOTPLUS kits according to the manufacturer’s instructions (Mabtech). IFN-γ ELISPOT assays were performed with mouse IFN-γ Precoated ELISPOT kits according to the manufacturer’s instructions (Dakewe Biotech) ...
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bioRxiv - Immunology 2021Quote: ... IL-2 (Cy3) and/or TNF (Cy5) were detected with IRIS FluoroSpot reader and counted with Apex software (Mabtech). Numbers of responding cells in negative controls were subtracted from stimulated samples to account for background responses ...
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bioRxiv - Immunology 2021Quote: ... cryopreserved PBMCs from study subjects were stimulated for 5 days with polyclonal B cell stimulation (1μg/mL R848 and 10 ng/mL rIL-2, Mabtech) in R10 media (RPMI 1640 with 10% FCS ...
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bioRxiv - Immunology 2023Quote: ... SARS-CoV-2 S antigen-specific T lymphocyte response in mice was assessed by IFN-γ ELISpotPLUS kits (Mabtech) following the manufacturer’s instruction ...
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bioRxiv - Microbiology 2022Quote: ... and the plate was incubated for 2 h at room temperature with R4-6A2 biotinylated anti-IFN-γ antibody (Mabtech) at the concentration of 100 μg/mL ...
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bioRxiv - Pathology 2022Quote: ... The cells were incubated for 20 hours at 37°C in 5% CO2 incubator in pre-coated Ferret IFNγ-ELISpot plates (3112-4APW-2, Mabtech). The assay was performed following the manufacturers protocol ...
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bioRxiv - Immunology 2020Quote: ... were measured using the Human IgG SARS-CoV-2 RBD ELISpotPLUS (ALP) kit according to the manufacturer’s protocol (Mabtech AB).
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bioRxiv - Immunology 2022Quote: ... Splenocytes were counted and 250,000 cells were plated per well into pre-covered plates and stimulated overnight with 10 μg/mL SARS-CoV-2 (S1) peptide pool (Mabtech) or medium as negative control or PHA 2 μg/mL as positive experimental control ...
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bioRxiv - Immunology 2023Quote: ... and the plate was incubated for 2 h at room temperature with R4-6A2 biotinylated anti-IFN-γ antibody (Mabtech) at the concentration of 100 μg/mL ...
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bioRxiv - Immunology 2023Quote: ... the 2CZ T cell hybridoma was used and the IL-2 secreted upon its activation was quantified using an ELISPOT assay (Mabtech) as previously described 7.
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bioRxiv - Immunology 2022Quote: The T cell immune responses in rhesus macaques were detected using the PBMCs with commercially available NHP IFN-γ and IL-2 ELISpot assay kits (Mabtech), and NHP IL-4 ELISpot assay kit (U-CyTech) ...
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bioRxiv - Microbiology 2020Quote: An IFNγ ELISpot assay was used to estimate the frequency and IFNγ production capacity of SARS-CoV-2-specific T cells in PBMCs using a human / simian IFNγ kit (MabTech, Nacka. Sweden), as described previously 46 ...
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bioRxiv - Cancer Biology 2021Quote: ... Plates were developed by washing 6 times in PBS/0.05%Tween 20 followed by incubation with biotinylated IFN-γ detection antibody (0.5 mg/mL; Mabtech; 2 hours, 37°C). This was followed by a further 6 washes in PBS/0.05%Tween 20 and incubation with avidin DH-coupled biotinylated peroxidase H complex (Vectastain Elite ABC Kit ...
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bioRxiv - Immunology 2020Quote: ... Cellular immune responses were measured by IFN-γ FluoroSpot assay according to the manufacturer’s instructions (Cat. No. FSP-4246-2 Mabtech, Inc., Cincinnati, OH). Briefly ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 antigen RBD01-dn5B was diluted to 80 mg/mL and used to coat wells of PVDF plates (Mabtech, #3654-WP-10) according to the assay kit protocol ...
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bioRxiv - Immunology 2022Quote: ... kits with pre-coated plates for enumeration of cells secreting IFN-γ and IL-17A were purchased from Mabtech (Cat. No. FSP-414443-2). Spots were enumerated on an AID iSpot EliSpot/ FluoroSpot Reader.
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bioRxiv - Immunology 2020Quote: ... An IFN-γ ELISpot assay was used to estimate the frequency and IFN-γ production capacity of SARS-CoV-2-specific T cells in PBMCs using a human/simian IFN-γ kit (MabTech, Nacka, Sweden). The cells were assayed at 2 x 105 cells per well ...
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bioRxiv - Immunology 2021Quote: ... The remaining time-points were evaluated using Monkey IFNγ/IL-4 FluoroSpot FLEX kit and Monkey IL-2 FluoroSpot FLEX kit (Mabtech, X-21A16B and X-22B) following manufacturer instructions ...
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bioRxiv - Immunology 2020Quote: ... Plates were substrated for 8-12 minutes using 0.5µM filtered 3’3’5’5’-Tetramethylbenzidine (TMB substrate) (Mabtech, 3652-F10) and the plates were evaluated with an AID automated microplate ELISpot reader and associated software (AID ELISpot Version 7.0).
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bioRxiv - Immunology 2022Quote: ... followed by 1 h incubation with AP-conjugated streptavidin (1:1000 in PBS, Mabtech). Plates were then washed and visualized using the VECTASTAIN® Elite® ABC-HRP kit according to the manufacturer’s instructions (Mabtech) ...
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bioRxiv - Immunology 2021Quote: ... (mAbs 1-D1K; Mabtech, Stockholm, Sweden) at a concentration of 10ug/mL ...
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bioRxiv - Immunology 2020Quote: ... Streptavidin-HRP (Mabtech – 1/1000 dilution) was added for 30 minutes at room temperature ...
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bioRxiv - Immunology 2022Quote: ... 100 µl of biotinylated secondary anti-IFN-γ antibody (1 µg/ml, clone 7-B6-1, Mabtech) in blocking buffer (PBS 0.5% FBS ...
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bioRxiv - Immunology 2022Quote: ... the plates were developed with human biotinylated IFN-γ detection antibody (7-B6-1, Mabtech; 1:2000), followed by incubation with Streptavidin-AP (Mabtech ...
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bioRxiv - Immunology 2024Quote: ... 100 µl of biotinylated secondary anti-IFN-γ antibody (1 µg/ml, clone 7-B6-1, Mabtech) in blocking buffer (PBS 0.5% FBS ...
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bioRxiv - Immunology 2020Quote: ... and incubated for 1 hour with streptavidin-HRP (diluted 1:100 with D-PBS, Mabtech, 3310-9-1000), and then washed again ...
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bioRxiv - Immunology 2022Quote: ... α-human IFN-γ (7-B6-1, MabTech); for fluorescence microscopy ...
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bioRxiv - Immunology 2019Quote: ... then added with alkaline phosphatase (ALP) labeled anti-human IFN-γ mAb (7-B6-1-ALP, 1:200; Mabtech) for 2 hours ...
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bioRxiv - Microbiology 2022Quote: ... Wells were then washed and treated for 1 h at room temperature with 1:1,000 diluted streptavidin-ALP from Mabtech. Afterwards ...
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bioRxiv - Immunology 2023Quote: ... Wells were then washed and treated for 1 h at room temperature with 1:1,000 diluted streptavidin-ALP from Mabtech. Afterwards ...
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bioRxiv - Immunology 2020Quote: ... the plates were developed with human biotinylated IFN-γ detection antibody (Clone 7-B6-1, 1 μg/ml, Mabtech, Sweden), followed by incubation with Streptavidin-AP (1:1000 ...
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bioRxiv - Immunology 2022Quote: ... anti-IFN-γ 7-B6-1 (biotinylated, Mabtech) as well as streptavidin-horseradish complex was added for visualization.
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bioRxiv - Immunology 2020Quote: ... 5% CO2 at a concentration of 1×105 cells/mL media on plates previously coated with anti-human IFNγ (Mab 1-D1K; Mabtech, Stockholm, Sweden). Cells were then discarded and plates were further incubated with biotinylated IFNγ antibody (Mab 7-B6-1 ...
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bioRxiv - Immunology 2020Quote: ... Controls included 1×105 effector CD8+ T cells co-cultured with anti-human CD3 monoclonal antibody (0.5 µg/mL, Mabtech, 3605-1-1000), or with media alone ...
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bioRxiv - Immunology 2021Quote: ... followed by streptavidin-alkaline phosphatase (Mabtech, 1:1,000 dilution). Spots were visualised using BCIP/NBT plus substrate and counted via ELISPOT plate reader.
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bioRxiv - Immunology 2021Quote: ... followed by streptavidin-alkaline phosphatase (Mabtech; 1:1,000 dilution). Spots were visualised using BCIP/NBT plus substrate (Mabtech ...
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bioRxiv - Immunology 2021Quote: ... M ORF3a and ORF7a proteins (#3622-1, Mabtech AB)57 ...
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bioRxiv - Bioengineering 2021Quote: ... with 1:1000 diluted streptavidine-ALP preparations from Mabtech. After washing ...
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bioRxiv - Cancer Biology 2021Quote: ... with 1:1000 diluted streptavidine-ALP preparations from Mabtech. After washing ...
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bioRxiv - Immunology 2020Quote: ... M ORF3a and ORF7a proteins (#3622-1, Mabtech AB) 19 ...
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bioRxiv - Immunology 2020Quote: ... with 1:1000 diluted streptavidine-ALP preparations from Mabtech. After washing ...
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bioRxiv - Immunology 2023Quote: ... The diluted detection antibody (1:1000) (MABTECH,3321-2H) was added and the plate was incubated at room temperature for 2h ...
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bioRxiv - Immunology 2023Quote: ... The diluted streptavidin-HRP (1:1000) (MABTECH,3321-2H) was added and the plate was incubated at room temperature for 1h ...
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bioRxiv - Immunology 2020Quote: ... Biotinylated secondary IFN-γ antibody (clone 7-B6-1, Mabtech) at 0.5μg/mL in PBS ...
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bioRxiv - Immunology 2020Quote: ... a 1:1,000 dilution of alkaline phosphatase-conjugated streptavidin (Mabtech) was added for 1 hr at room temperature ...
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bioRxiv - Immunology 2020Quote: ... or 3) anti-CD3 positive control (Mabtech, 1:1000 dilution). All samples were plated in triplicate ...
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bioRxiv - Immunology 2021Quote: ... or 3) anti-CD3 positive control (Mabtech, 1:1000 dilution), in triplicate ...
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bioRxiv - Immunology 2020Quote: ... were coated with human IFN-γ antibody (1-D1K, MabTech) overnight at 4°C ...