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Citations for Mabtech :
151 - 200 of 209 citations for 7 PHENYL 1 2 4 TRIAZOLO 1 5 A PYRIMIDINE 6 CARBONITRILE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Each peptide pool (2 µg/ml) and αCD28 (0.1 µg/ml, Mabtech) were added to the cells and plates were incubated for 48h at 37°C ...
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bioRxiv - Immunology 2021Quote: ... were coated with monoclonal antibodies to bovine IFNγ and IL-2 (MABTECH). Duplicate wells were seeded with 1×105 cells per well of cryopreserved bovine PBMCs (rested overnight before use ...
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bioRxiv - Cancer Biology 2023Quote: ... and Granzyme B (Biozol, #3485-1H-20 for CD276- and CD19-CAR T cells; MabTech, #3486-1H-6 for F8-FR4-CAR T cells) following the manufactures’ instructions ...
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bioRxiv - Immunology 2023Quote: TNFα ELISA was performed with the supernatant from T cell and LCL co-culture as described above using the human TNFα ELISABASIC kit (Mabtech, Cat. 3512-1H-6), following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... Anti-IFNγ and anti-IL-4 ELISpot PLUS kits were purchased from Mabtech (Nacka Strand, Sweden). PepTivator Ovalbumin epitope mix was provided by Miltenyi Biotec (Nordrhein-Westfalen ...
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bioRxiv - Immunology 2021Quote: ... Plates were then washed and incubated with biotinylated anti-mouse IFN-γ antibody (clone R4-6A2; Mabtech; 1 μg/mL) followed by streptavidin-alkaline phosphatase (Mabtech ...
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bioRxiv - Immunology 2020Quote: ... Plates were washed 6X with PBS and incubated with 50 µl of streptavidin-alkaline phosphatase conjugate (1:1000 dilution; Mabtech) diluted in PBS 1% FBS ...
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bioRxiv - Cancer Biology 2021Quote: ... and the wells incubated with 100 μL of 1 μg/ml of the R4-6A2 biotinylated anti-IFN-γ (Mabtech) for 2 hours at r.t ...
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bioRxiv - Immunology 2020Quote: ... anti-human IgA-490 (green fluorescence) and anti-human IgM-640 (red fluorescence) secondary detection antibodies (diluted 1:500 each) (all antibodies from Mabtech). Fluorescent spots indicating a single ASC were detected with an IRIS FluoroSpot reader and counted with Apex software (Mabtech).
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bioRxiv - Immunology 2024Quote: ... Plates were washed 6x times with PBST and plated with streptavidin-HRP conjugate (1:1000 dilution; catalogue #3310-9-1000, Mabtech) for 1 hour at room temperature in the dark ...
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bioRxiv - Immunology 2021Quote: ... ELISpotPLUS plates as well as Mouse IFNγ ELISpotPRO plates (MABTECH, 3421M-2AST-2) were washed with PBS and blocked overnight at 4°C in RPMI 1640 media (Invitrogen ...
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Dendritic cells focus CTL responses toward highly conserved and topologically important HIV epitopesbioRxiv - Immunology 2020Quote: ... T cells were resuspended at a concentration of 3×105 cells per milliliter and 100 μl (3×104 cells/well) were transferred to IFNγ-antibody coated 96-well ELISpot plates (5μg/ml anti-IFNγ mAb 1-D1K, Mabtech, Stockholm, Sweden). Individual 9mer peptide dilutions were done in a separate 1 ml deep-well 96-well plate at a final concentration of 2μg/ml and 100 μl of this dilution was added to T cell-containing wells to give a final peptide concentration of 1μg/ml ...
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bioRxiv - Immunology 2020Quote: ... IFN-γ spot forming cells (SFC) were detected by staining membranes with anti-mouse IFN-γ biotin mAb (1 µg/mL; clone R46A2, Mabtech) followed by streptavidin-alkaline phosphatase (1 µg/mL ...
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bioRxiv - Microbiology 2023Quote: ... The plates were incubated at 37 °C in 5 % CO2 for 18 h and washed with PBS before the addition of 1 µg/ml biotinylated anti-IFN-γ mAb (Mabtech) at room temperature for 2 h ...
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bioRxiv - Immunology 2020Quote: Rhesus macaque PBMCs were tested with commercially available NHP IFNγ and IL-4 ELISpot assay kits (Mabtech). Cryopreserved rhesus macaque PBMCs were thawed in pre-warmed AIM-V media (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... ELISpot was performed using mouse IFN-γ and IL-4 ELISpot PLUS kits (MABTECH, Cincinnati, OH, USA) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... ELISpot assays for IFNγ were performed as instructed by the manufacturer using the Mouse IFNγ ELISpot Set (MabTech, cat# Q00824-1×20210623DS). 0.1×106 mouse splenocytes were plated into each well and stimulated overnight with 0.2 µg per peptide per well of RBD-derived overlapping peptides (GenScript).
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bioRxiv - Immunology 2020Quote: ... for the enumeration of total IgG-secreting cells with 1 µg per well of anti-porcine IgG (mAb, MT421, Mabtech AB, Sweden) or with culture medium supplemented with 10% FBS (background) ...
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bioRxiv - Immunology 2020Quote: ... and for the enumeration of total IgG-secreting cells with 1 µg per well of anti-porcine IgG (mAb, MT421, Mabtech AB, Sweden) or with culture medium supplemented with 10% FBS (media background control) ...
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bioRxiv - Immunology 2020Quote: ... were coated with 50 μl of anti-mouse IFN-γ mAb AN18 (5 µg/ ml; Mabtech) overnight at 4°C before being washed and blocked with cRMPI 10% FBS for 1 hr at 37C ...
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bioRxiv - Microbiology 2023Quote: ... that had been precoated with 5 µg/ml anti-IFN-γ monoclonal antibody (mAb) AN18 (Mabtech). The plates were incubated at 37 °C in 5 % CO2 for 18 h and washed with PBS before the addition of 1 µg/ml biotinylated anti-IFN-γ mAb (Mabtech ...
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bioRxiv - Immunology 2020Quote: Rhesus macaque PBMCs were tested with commercially available NHP IFNγ and IL-4 ELISpot assay kits (Mabtech, Sweden). Cryopreserved rhesus macaque PBMCs were thawed in pre-warmed AIM-V media (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... were pre-wet with 30% ethanol for 60 seconds and then coated with 100 µl primary anti-IFN-γ antibody solution (10 µg/ml of 1-D1K coating antibody from Mabtech, Cincinnati, OH) OVN at 4°C ...
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bioRxiv - Immunology 2024Quote: ... were pre-wet with 30% ethanol for 60 seconds and then coated with 100 µl primary anti-IFN-γ antibody solution (10 µg/ml of 1-D1K coating antibody from Mabtech, Cincinnati, OH) OVN at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... or IL-5 were determined using a mouse enzyme-linked immunospot (ELISpot) assay kit (Mabtech, Cincinnati, OH) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... 96 well plates were coated with a mixture of antibodies against IFN-α (Mabtech, MT1/3/5) overnight at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Plates contained additional no target cell negative control and anti-CD3 antibody (CD3-2, Mabtech) positive control wells ...
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bioRxiv - Bioengineering 2023Quote: ... IFN-γ and IL-2-secreting cells were detected using ELISpot kits (DAKEWE and MABTECH) according to the manufacture protocol and spots were counted using an ELISpot Analyzer AT-SPOT(SINSAGE).
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bioRxiv - Immunology 2020Quote: ... plates were coating with 50 μL of anti-porcine IFN-γ mAb pIFNγ-I (5 µg/ ml; Mabtech) and incubated with 50 μL of biotinylated anti-porcine IFN-γ mAb P2C11 (1:1000 dilution ...
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bioRxiv - Immunology 2020Quote: ... and 5 μg/mL anti-perforin capture antibody mix (capture αPrf; Mabtech, combination of clones Pf-80/164). The resulting surfaces were micropatterned as described before with 10 μg/mL αLFA-1 + 10 μg/mL αCD16 + 5 μg/mL capture αPrf + 10 μg/mL BSA-AF555 ...
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bioRxiv - Immunology 2023Quote: ... Commercially available ELISPOT plates for evaluation of IL-4 (MuIL4, Immunospot, Cellular Technology Limited) and IFNg (MuIFNg, Immunospot, Cellular Technology Limited; cat# 3110-4APW-10, rabbit, MabTech) were used ...
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bioRxiv - Immunology 2024Quote: ... Frequencies of IFN-γ or IL-2-secreting T cells were assessed using commercial kits (Mabtech) according to the manuals ...
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bioRxiv - Biochemistry 2020Quote: ... serum samples were centrifuged at 10000 rpm for 20 minutes at 4 °C and supernatants were analyzed by human ApoA-I ELISA (3710-1HP-10, Mabtech Inc.), as well as by denaturant and native western blot (procedure is described in the Supplementary Information file).
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bioRxiv - Bioengineering 2023Quote: ... were collected for ELISpot assays using ELISpot Plus: Monkey IFN-γ (HRP) and ELISpot Plus: Human IL-4 (HRP) kits (Mabtech). 2 × 105 PBMCs seeded onto 96 well plates in the kits were incubated with 1 μg of PepMixTM SARS-CoV-2 (Spike Glycoprotein ...
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bioRxiv - Immunology 2020Quote: ... The detection antibody solution composed of PBS + 5 μg/mL anti-perforin conjugated with biotin (clone Pf-344, Mabtech) was added to the slide chambers ...
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bioRxiv - Immunology 2022Quote: A fluorospot 96-well plate from a Human INF-γ/IL-2 FluorospotPLUS kit (Mabtech AB, Sweden) was washed x3 with PBS and then blocked with optimizer media-10% FCS according to manufacturer description at room temperature until use ...
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bioRxiv - Immunology 2021Quote: ELISpot assays were performed with mouse IFN-γ and IL-4 ELISpot PLUS kits according to the manufacturer’s instructions (3321-4HPT-10 and 3311-4HPW-10, Mabtech, Herndon, VA, USA). A total of 2,5 × 105 or 4 x 105 splenocytes from female mice were seeded per well for the IFN-γ and IL-4 tests respectively and ex vivo stimulated either with the 1:1 mix of the RBD overlapping peptides from the B.1.1.7 (alpha variant ...
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bioRxiv - Immunology 2019Quote: ... were co-cultured with GLC peptide-specific T cells (5×104 cells/ per well) for 20 hours as per manufacturer’s protocol (Mabtech, Sweden).
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bioRxiv - Immunology 2019Quote: ... NS1-specific IFNγ and IL-5 production by ZIKV-specific splenic T cells were enumerated by Enzyme Linked ImmunoSpot (ELISPOT) assay following manufacturer’s instructions (Mouse IFN-γ/IL-5 ELISpot Basic kits; Mabtech). Mouse splenocytes were left unstimulated or stimulated with an NS1 peptide pool (PepMix ZIKV NS1 Ultra ...
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bioRxiv - Immunology 2020Quote: ... were pre-wet with 70% methanol and then coated with 100 µl primary anti-IFN-γ antibody solution (10 µg/ml of 1-D1K coating antibody from Mabtech in PBS, pH 7.4, V-E4) OVN at 4°C ...
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bioRxiv - Immunology 2021Quote: ... and 2 x 105 splenocytes were plated into each well of the Mouse IFN-γ ELISpotPLUS plates (MabTech). The splenocytes were stimulated for 20 hours at 37°C with RBD peptides (15-mer peptides overlapping by 9 amino acid spanning the RBD of SARS-CoV-2 spike protein ...
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bioRxiv - Immunology 2021Quote: ... 2×105 cells were added to each well of pre-coated Immobilon-FL PVDF 96-well plates (Mabtech) in the presence of 2 μg/ml peptide pool ...
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bioRxiv - Immunology 2023Quote: ... 2×103 transfected T cells were plated per well in a pre-coated 96-well ELISpot plate (Mabtech) together with 5×104 PHA-activated autologous CD4 T cells as antigen-presenting cells and challenged with the specific peptide ...
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bioRxiv - Immunology 2023Quote: ... FluoroSpot assays were performed according to the manufacturer’s recommendations (FluoroSpot Plus: Human IFNγ/IL-2/TNFα kit, MABTech). Briefly ...
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bioRxiv - Immunology 2021Quote: ... IL-2 (Cy3) and/or TNF (Cy5) were detected with IRIS FluoroSpot reader and counted with Apex software (Mabtech). Numbers of responding cells in negative controls were subtracted from stimulated samples to account for background responses ...
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bioRxiv - Immunology 2023Quote: ... SARS-CoV-2 S antigen-specific T lymphocyte response in mice was assessed by IFN-γ ELISpotPLUS kits (Mabtech) following the manufacturer’s instruction ...
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bioRxiv - Immunology 2021Quote: Murine IFN-γ and IL-5 ELISpot assays were performed following the manufacturer’s procedures for mouse IFN-γ and IL-5 ELISpot kits (3321-2H and 3321-2A, Mabtech, Cincinnati, OH). Briefly ...
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bioRxiv - Microbiology 2022Quote: ... and the plate was incubated for 2 h at room temperature with R4-6A2 biotinylated anti-IFN-γ antibody (Mabtech) at the concentration of 100 μg/mL ...
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bioRxiv - Immunology 2020Quote: ... were measured using the Human IgG SARS-CoV-2 RBD ELISpotPLUS (ALP) kit according to the manufacturer’s protocol (Mabtech AB).
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bioRxiv - Immunology 2022Quote: ... Splenocytes were counted and 250,000 cells were plated per well into pre-covered plates and stimulated overnight with 10 μg/mL SARS-CoV-2 (S1) peptide pool (Mabtech) or medium as negative control or PHA 2 μg/mL as positive experimental control ...