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Citations for Mabtech :
51 - 100 of 195 citations for 6 Chloro 2 3 diphenylimidazo 1 2 b pyridazine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... coated with AN18 IFNγ (1 µg/ml, Mabtech #3321-3-250;) and 11B11 IL4 (1 µg/ml ...
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bioRxiv - Immunology 2021Quote: ... and envelope glycoprotein B was purchased from Mabtech.
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bioRxiv - Immunology 2022Quote: Single cell suspensions from lungs and spleens of mice were stimulated with mouse IL-2 (R & D Systems, Cat # 402-ML; 0.5 μg/mL) and RD848 (Mabtech, Cat # 3611-5X; 1 μg/mL) for 3 days to induce nonspecific polyclonal expansion ...
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bioRxiv - Immunology 2021Quote: ... Plates contained additional no target cell negative control and anti-CD3 antibody (CD3-2, Mabtech) positive control wells ...
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bioRxiv - Bioengineering 2023Quote: ... IFN-γ and IL-2-secreting cells were detected using ELISpot kits (DAKEWE and MABTECH) according to the manufacture protocol and spots were counted using an ELISpot Analyzer AT-SPOT(SINSAGE).
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bioRxiv - Immunology 2024Quote: ... Frequencies of IFN-γ or IL-2-secreting T cells were assessed using commercial kits (Mabtech) according to the manuals ...
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bioRxiv - Immunology 2020Quote: ... or biotinylated secondary anti-granzyme B antibody (clone GB11, Mabtech) at 1.0μg/mL in PBS was added to the plates to a final volume of 100μL and incubated for 1 hour in the dark ...
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bioRxiv - Immunology 2022Quote: A fluorospot 96-well plate from a Human INF-γ/IL-2 FluorospotPLUS kit (Mabtech AB, Sweden) was washed x3 with PBS and then blocked with optimizer media-10% FCS according to manufacturer description at room temperature until use ...
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bioRxiv - Immunology 2021Quote: ... and 2 x 105 splenocytes were plated into each well of the Mouse IFN-γ ELISpotPLUS plates (MabTech). The splenocytes were stimulated for 20 hours at 37°C with RBD peptides (15-mer peptides overlapping by 9 amino acid spanning the RBD of SARS-CoV-2 spike protein ...
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bioRxiv - Immunology 2021Quote: ... 2×105 cells were added to each well of pre-coated Immobilon-FL PVDF 96-well plates (Mabtech) in the presence of 2 μg/ml peptide pool ...
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bioRxiv - Immunology 2023Quote: ... 2×103 transfected T cells were plated per well in a pre-coated 96-well ELISpot plate (Mabtech) together with 5×104 PHA-activated autologous CD4 T cells as antigen-presenting cells and challenged with the specific peptide ...
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bioRxiv - Immunology 2023Quote: ... FluoroSpot assays were performed according to the manufacturer’s recommendations (FluoroSpot Plus: Human IFNγ/IL-2/TNFα kit, MABTech). Briefly ...
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bioRxiv - Immunology 2021Quote: ... and IL-8 (3114-1A-6 MABTECH) release in 24h culture supernatants using the provided manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: IL-4 and IL-2 ELISPOT assays were performed with mouse IL-4 ELISPOTPLUS kits and mouse IL-2 ELISPOTPLUS kits according to the manufacturer’s instructions (Mabtech). IFN-γ ELISPOT assays were performed with mouse IFN-γ Precoated ELISPOT kits according to the manufacturer’s instructions (Dakewe Biotech) ...
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bioRxiv - Immunology 2021Quote: ... IL-2 (Cy3) and/or TNF (Cy5) were detected with IRIS FluoroSpot reader and counted with Apex software (Mabtech). Numbers of responding cells in negative controls were subtracted from stimulated samples to account for background responses ...
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bioRxiv - Immunology 2023Quote: ... SARS-CoV-2 S antigen-specific T lymphocyte response in mice was assessed by IFN-γ ELISpotPLUS kits (Mabtech) following the manufacturer’s instruction ...
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bioRxiv - Immunology 2021Quote: ... were measured using a multicolor B cell FluoroSpot kit with modifications (Mabtech). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... or IgA (Cat. 3860-1AD-6, Mabtech, USA) ranging from 1.2 μg/ml to 0,0047 μg/ml ...
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bioRxiv - Microbiology 2022Quote: ... and the plate was incubated for 2 h at room temperature with R4-6A2 biotinylated anti-IFN-γ antibody (Mabtech) at the concentration of 100 μg/mL ...
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bioRxiv - Pathology 2022Quote: ... The cells were incubated for 20 hours at 37°C in 5% CO2 incubator in pre-coated Ferret IFNγ-ELISpot plates (3112-4APW-2, Mabtech). The assay was performed following the manufacturers protocol ...
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bioRxiv - Immunology 2020Quote: ... were measured using the Human IgG SARS-CoV-2 RBD ELISpotPLUS (ALP) kit according to the manufacturer’s protocol (Mabtech AB).
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bioRxiv - Immunology 2022Quote: ... Splenocytes were counted and 250,000 cells were plated per well into pre-covered plates and stimulated overnight with 10 μg/mL SARS-CoV-2 (S1) peptide pool (Mabtech) or medium as negative control or PHA 2 μg/mL as positive experimental control ...
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bioRxiv - Immunology 2023Quote: ... and the plate was incubated for 2 h at room temperature with R4-6A2 biotinylated anti-IFN-γ antibody (Mabtech) at the concentration of 100 μg/mL ...
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bioRxiv - Immunology 2023Quote: ... the 2CZ T cell hybridoma was used and the IL-2 secreted upon its activation was quantified using an ELISPOT assay (Mabtech) as previously described 7.
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bioRxiv - Immunology 2022Quote: ... with 40 μg/mL anti-human IFNγ mAb 1-D1K (MABTECH, Stockholm, Sweden, Cat #3420-3-250) for 30 minutes followed by two washes ...
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bioRxiv - Cell Biology 2024Quote: ... APOA1 (3710-3-1000, Mabtech) and ALIX (2171S ...
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bioRxiv - Cell Biology 2024Quote: ... APOB100 (3715-3-250, Mabtech), APOA1 (3710-3-1000 ...
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bioRxiv - Immunology 2020Quote: ... and seeded on the IFN-γ/Granzyme-B/TNF-α FluoroSpot plates (Mabtech) at 200,000 cells/well in RPMI 1640 with 10% Human Serum HI ...
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bioRxiv - Immunology 2022Quote: The T cell immune responses in rhesus macaques were detected using the PBMCs with commercially available NHP IFN-γ and IL-2 ELISpot assay kits (Mabtech), and NHP IL-4 ELISpot assay kit (U-CyTech) ...
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bioRxiv - Immunology 2022Quote: ... and BVD6-24G2 anti-IL4 (Mabtech #3311-6-250) detection antibody ...
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bioRxiv - Cancer Biology 2023Quote: ... and human IgG ELISA kit (Mabtech, #3850-1H-6).
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bioRxiv - Immunology 2023Quote: ... washed and coated overnight with anti-IFN-ψ coating antibody (mAb-1-D1K, Mabtech, Cat. 3420-3-1000). Plates were washed and T cells were co-cultured with autologous LCL in RPMI/ 10% FCS/ 1% Pen/Strep at an effector ...
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bioRxiv - Immunology 2022Quote: Incubate the array with 10 μg/mL biotinylated anti-human IFNγ 7-B6-1 (MABTECH AB, Stockholm, Sweden, Cat #3420-6-250) and then 12 μg/mL AF647-Streptavidin (Jackson ImmunoResearch ...
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bioRxiv - Microbiology 2020Quote: An IFNγ ELISpot assay was used to estimate the frequency and IFNγ production capacity of SARS-CoV-2-specific T cells in PBMCs using a human / simian IFNγ kit (MabTech, Nacka. Sweden), as described previously 46 ...
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bioRxiv - Cancer Biology 2021Quote: ... Plates were developed by washing 6 times in PBS/0.05%Tween 20 followed by incubation with biotinylated IFN-γ detection antibody (0.5 mg/mL; Mabtech; 2 hours, 37°C). This was followed by a further 6 washes in PBS/0.05%Tween 20 and incubation with avidin DH-coupled biotinylated peroxidase H complex (Vectastain Elite ABC Kit ...
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bioRxiv - Immunology 2020Quote: ... Cellular immune responses were measured by IFN-γ FluoroSpot assay according to the manufacturer’s instructions (Cat. No. FSP-4246-2 Mabtech, Inc., Cincinnati, OH). Briefly ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 antigen RBD01-dn5B was diluted to 80 mg/mL and used to coat wells of PVDF plates (Mabtech, #3654-WP-10) according to the assay kit protocol ...
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bioRxiv - Immunology 2022Quote: ... kits with pre-coated plates for enumeration of cells secreting IFN-γ and IL-17A were purchased from Mabtech (Cat. No. FSP-414443-2). Spots were enumerated on an AID iSpot EliSpot/ FluoroSpot Reader.
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bioRxiv - Immunology 2020Quote: ... An IFN-γ ELISpot assay was used to estimate the frequency and IFN-γ production capacity of SARS-CoV-2-specific T cells in PBMCs using a human/simian IFN-γ kit (MabTech, Nacka, Sweden). The cells were assayed at 2 x 105 cells per well ...
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bioRxiv - Immunology 2021Quote: ... goat anti-mouse IgA biotin (Mabtech, Cat no. 3865-6-250) at (1:500 ...
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bioRxiv - Immunology 2021Quote: ... The remaining time-points were evaluated using Monkey IFNγ/IL-4 FluoroSpot FLEX kit and Monkey IL-2 FluoroSpot FLEX kit (Mabtech, X-21A16B and X-22B) following manufacturer instructions ...
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bioRxiv - Immunology 2020Quote: ... the plate was washed 6 times and the pNPP solution (Mabtech, Sweden) was added at 50 μl per well ...
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bioRxiv - Immunology 2023Quote: The number of B cells secreting IgG was quantified using a mouse ELISpot kit (Mabtech, 3825-2H). The isolated cells were counted using the Countess Automated Cell Counter II (ThermoFisher ...
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bioRxiv - Immunology 2020Quote: ... and immediately washed 6x with 200μL ddH2O and then coated with 100μL/well of PBS + 15μg/mL primary anti-human granzyme B antibody (clone GB10, Mabtech) overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: ... The following morning plates were washed with 1x PBS-T before being coated with 3 μg/mL streptavidin-ALP secondary antibody (Mabtech, clone 7-B6-1) and incubated for 2 hours at room temperature ...
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bioRxiv - Immunology 2021Quote: ... followed by application of IFN-γ-detection antibody (MABTECH Cat# 3420-6-250, RRID:AB_907273) and visualization by Streptavidin horseradish peroxidase (HRP ...
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bioRxiv - Immunology 2022Quote: ... 100 µL of either goat anti-mouse IgG (for 6E2 hybridoma cells) or mouse anti-human IgG (for EBV-immortalized human B cells) specific for the Fc region (Mabtech) diluted in sterile PBS at 15 µg/mL were added to wells followed by incubation at 4°C for 24 hours ...
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bioRxiv - Immunology 2021Quote: ... and IgA production by each sorted B-cell subset were evaluated by using the Human IgG/IgM and IgG/IgA FluoroSpot kits (MABTECH), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... IgG- and IgM-ASCs in freshly isolated PBMCs were measured using a multicolor B cell FluoroSpot kit with modifications (Mabtech). Briefly ...
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bioRxiv - Immunology 2021Quote: ... IFN-α was quantified from culture media using a pan-subtype ELISA (MABTECH 3425-1H-6).