Labshake search
Citations for Mabtech :
151 - 200 of 200 citations for Mouse B And T Lymphocyte Attenuator BTLA ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... we used antigen-specific IFN-γ ELISpot kits (MABTECH, 3321-4HPT-10) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... and PN-specific IgG ELISpots were performed with commercially available kits (Mabtech), as per manufacturer’s recommendations.
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bioRxiv - Immunology 2022Quote: ... ELISpot assays were performed using Monkey IFNγ ELISpotPLUS kits (MabTech, cat# 3241M-4HPW). Precoated 96-well ELISpot plates were blocked with RPMI + 10% FBS for 2 hours at room temperature ...
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bioRxiv - Microbiology 2019Quote: ... Antigen-specific IgG ELISpot assays were performed using Human IgG ELISpotBASIC kit (MabTech). PBMCs were first pre-stimulated with a mixture of R848 at 1 μg/ml and rhIL-2 at 10 ng/ml for 96h ...
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bioRxiv - Immunology 2022Quote: Single cell suspensions from lungs and spleens of mice were stimulated with mouse IL-2 (R & D Systems, Cat # 402-ML; 0.5 μg/mL) and RD848 (Mabtech, Cat # 3611-5X; 1 μg/mL) for 3 days to induce nonspecific polyclonal expansion ...
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bioRxiv - Immunology 2022Quote: ... Cell suspensions were transferred to pre-coated Human IFN-γ ELISpot plus kit (Mabtech) and developed after 20h according to manufacturer instructions ...
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bioRxiv - Microbiology 2019Quote: ... IFN-γ and IL-17A ELISPOT assays were performed using Human ELISpotplus kit (MabTech) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... cells were cultured for 24 h and detected using a commercial kit (MABTECH, Nacka, Sweden) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... IFN-γ and IL-2-secreting cells were detected using ELISpot kits (DAKEWE and MABTECH) according to the manufacture protocol and spots were counted using an ELISpot Analyzer AT-SPOT(SINSAGE).
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bioRxiv - Immunology 2024Quote: ... IFNγ and TNFα ELISpot were performed using monkey cytokine specific ELISpotPLUS (ALP) KITs (Mabtech, Inc.) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... the cells were washed and plated onto PVDF ELISpot filter plates coated with anti-mouse IgG or IgA capture antibody (Mabtech, Cat# BASIC 3825-2H and BASIC 3835-2H). The plates were incubated at 37°C for 24 hours ...
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bioRxiv - Immunology 2021Quote: ... ELISpot assays were performed using the Rat IFN-γ ELISpotPLUS kit (MABTECH, Cat. No.: 3220- 4APW), Rat IL-4 T cell ELISpot kit (U-CyTech ...
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bioRxiv - Immunology 2020Quote: An ELISPOT assay was performed with the Monkey IFN-γ ELISPOT Kit (Mabtech, Cincinnati, OH, USA) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... Anti-IFNγ and anti-IL-4 ELISpot PLUS kits were purchased from Mabtech (Nacka Strand, Sweden). PepTivator Ovalbumin epitope mix was provided by Miltenyi Biotec (Nordrhein-Westfalen ...
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bioRxiv - Immunology 2023Quote: IFN-γ ELISpot assays were performed using the Human IFN-γ ELISpot PLUS ALP kit (Mabtech) according to the manufacturer’s protocol with some modifications ...
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bioRxiv - Immunology 2020Quote: Rhesus macaque PBMCs were tested with commercially available NHP IFNγ and IL-4 ELISpot assay kits (Mabtech). Cryopreserved rhesus macaque PBMCs were thawed in pre-warmed AIM-V media (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ELISpot assays were performed using precoated monkey IFN-γ ELISpotPLUS Kits according to the manufacturer’s instructions (Mabtech). Briefly ...
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bioRxiv - Immunology 2022Quote: A fluorospot 96-well plate from a Human INF-γ/IL-2 FluorospotPLUS kit (Mabtech AB, Sweden) was washed x3 with PBS and then blocked with optimizer media-10% FCS according to manufacturer description at room temperature until use ...
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bioRxiv - Immunology 2023Quote: ... ELISpot assay was performed on treated PBMCs using ELISpot Pro: Human IFN-γ (ALP) kit (Mabtech, Sweden), following manufacture’s protocol ...
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bioRxiv - Immunology 2020Quote: Rhesus macaque PBMCs were tested with commercially available NHP IFNγ and IL-4 ELISpot assay kits (Mabtech, Sweden). Cryopreserved rhesus macaque PBMCs were thawed in pre-warmed AIM-V media (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... IFN-γ secreting cells were detected by Human IFN-γ ELISpot Basic Kit (ALP) (Mabtech AB, 3420-2A). The plates were developed using the AP Conjugate Substrate Kit (Biorad ...
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bioRxiv - Immunology 2022Quote: ... Cell suspensions were transferred to pre-coated Human IFN-γ ELISpot plus kit plates (Mabtech, Nacka Strand, Sweden) and developed after 20h per manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... FluoroSpot assays were performed according to the manufacturer’s recommendations (FluoroSpot Plus: Human IFNγ/IL-2/TNFα kit, MABTech). Briefly ...
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bioRxiv - Immunology 2024Quote: ... we conducted ex vivo ELISpot assay using equine interferon-γ (IFN-γ) ELISpot kit (Mabtech, Inc.; Cincinnati, Ohio) according to manufacturer’s directions ...
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bioRxiv - Microbiology 2023Quote: ... IFN-γ+ ELISPOTs assays were performed using 105 cells/well with the Hamster IFN-γ ELISPOTBASIC kit (Mabtech AB) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... The ELISpot assay was performed on treated PBMCs using the ELISpot Pro: Human IFN-g (ALP) kit (Mabtech, Sweden), following the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... were measured using the Human IgG SARS-CoV-2 RBD ELISpotPLUS (ALP) kit according to the manufacturer’s protocol (Mabtech AB).
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bioRxiv - Immunology 2022Quote: ... and cellular responses were determined on fresh PBMCs at Charles River Laboratories using a monkey IFN-γ ELISpot kit (Mabtech) at Weeks 1 ...
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bioRxiv - Immunology 2023Quote: ... plates were developed using reagents and following procedures and incubation periods described in the pig IFNγ ELISpot Plus kit (3130-4HPW-10, Mabtech). Spots were counted under a dissection microscope (Leica GZ6) ...
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bioRxiv - Microbiology 2024Quote: ... Interferon-gamma (IFN-γ) and interleukin-4 (IL-4) were quantified with commercially-available kits (MABTECH AB and FineTest, respectively) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: The T cell immune responses in rhesus macaques were detected using the PBMCs with commercially available NHP IFN-γ and IL-2 ELISpot assay kits (Mabtech), and NHP IL-4 ELISpot assay kit (U-CyTech) ...
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bioRxiv - Cancer Biology 2019Quote: ... This method is similar to before(Depla et al., 2008) and were performed using a Human IFN-gamma ELISpot PLUS kit (Mabtech, Sweden) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2021Quote: ... The number of B cells secreting SARS-CoV-2 RBD-specific IgG and total IgG were measured using the Human IgG SARS-CoV-2 RBD ELISpotPLUS kit (Mabtech AB)6.
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bioRxiv - Immunology 2021Quote: Antigen-specific circulating PBMCs were evaluated using pre-coated FluoroSpot plates and kits purchased from Mabtech (Mabtech AB, Nacka Strand, Sweden) and used according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: Total splenocytes were cultured on anti-IgG ELISpot plates and developed using the standardized ELISpot kit (Mabtech Inc., Cincinnati, OH, USA). Briefly ...
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bioRxiv - Immunology 2020Quote: IFN-γ and IL-2 secreting T cells were detected using Human IFN-γ/IL-2 SARS-CoV-2 FluoroSpotPLUS kits according to the manufacture’s protocol (Mabtech AB). The plates pre-coated with capturing monoclonal anti-IFN-γ and anti-IL-2 were incubated overnight in RPMI-1640 medium containing 10% FCS supplemented with a mixture containing the SARS-CoV-2 peptide pool (scanning or defined pools) ...
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bioRxiv - Immunology 2020Quote: IFNy ELISpot assay of PBMCs was performed using the Monkey IFNy ELISpot PRO kit (Mabtech Monkey IFNy ELISPOT pro, #3421M-2APT) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... at an E:T ratio of 5:1 for 24 hours followed by measurement of specific cytokine production using the human IL-2 ELISpot kit (Mabtech AB) and ELIPHOTO Counter (Minerva Tech).
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bioRxiv - Immunology 2021Quote: To assess memory T cell responses to SARS-CoV-2 in convalescent COVID-19 patient samples we utilized Human IFN-γ/IL-2/TNF FluoroSpot kit (Mabtech), which allows for the detection of T cells producing IFN-γ ...
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bioRxiv - Immunology 2022Quote: ... Plates were then washed and visualized using the VECTASTAIN® Elite® ABC-HRP kit according to the manufacturer’s instructions (Mabtech). Antigen-specific T cell responses were quantified by subtracting the number of spots in unstimulated cells from the peptide stimulated cells ...
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bioRxiv - Bioengineering 2023Quote: ... were collected for ELISpot assays using ELISpot Plus: Monkey IFN-γ (HRP) and ELISpot Plus: Human IL-4 (HRP) kits (Mabtech). 2 × 105 PBMCs seeded onto 96 well plates in the kits were incubated with 1 μg of PepMixTM SARS-CoV-2 (Spike Glycoprotein ...
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bioRxiv - Immunology 2019Quote: The ex vivo IFN-γ ELISpot assay was performed as previously described using a human IFN-γ ELISpot kit (capture mAb -D1K) (Mabtech) 8 ...
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bioRxiv - Microbiology 2020Quote: ... Baboon IFN-γ and IL-4 assays were carried out using NHP IFN-γ and Human IL-4 assay kit from Mabtech using PBMC collected at day 7 following the second immunization (day 28).
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bioRxiv - Microbiology 2019Quote: Freshly isolated PBMCs (2-2.5 × 105/well) were stimulated for 16-18 hours in a 96-well pre-coated plate (ELISpotPLUS kit, MABTECH Inc., Mariemont, OH) at 37°C in a 5% CO2 incubator ...
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bioRxiv - Microbiology 2021Quote: ... pulsed with WT1328-348 (10 µg/ml) followed by measurement of specific cytokine production using the human IFN-γ ELISpot kit (Mabtech AB) and ELIPHOTO Counter (Minerva Tech).
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bioRxiv - Immunology 2021Quote: ... baboon IFN-γ and IL-4 assays were carried out using NHP IFN-γ and Human IL-4 assay kits from Mabtech. Assays were performed in triplicate.
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bioRxiv - Immunology 2021Quote: ELISpot assays were performed with mouse IFN-γ and IL-4 ELISpot PLUS kits according to the manufacturer’s instructions (3321-4HPT-10 and 3311-4HPW-10, Mabtech, Herndon, VA, USA). A total of 2,5 × 105 or 4 x 105 splenocytes from female mice were seeded per well for the IFN-γ and IL-4 tests respectively and ex vivo stimulated either with the 1:1 mix of the RBD overlapping peptides from the B.1.1.7 (alpha variant ...
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bioRxiv - Immunology 2021Quote: Hamster interferon gamma (IFN-γ) enzyme-linked immunosorbent spot (ELISpot) analysis was performed using the Hamster IFN-γ ELISpotBASIC kit (MABTECH, Nacka Strand, Sweden) in combination with multiscreen immunoprecipitation (IP ...
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bioRxiv - Immunology 2022Quote: ... kits with pre-coated plates for enumeration of cells secreting IFN-γ and IL-17A were purchased from Mabtech (Cat. No. FSP-414443-2). Spots were enumerated on an AID iSpot EliSpot/ FluoroSpot Reader.
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bioRxiv - Microbiology 2024Quote: ... The diluted plasma was determined to determine IFN-γ levels using a commercial monkey IFN-γ ELISApro kit (catalog no. M4210M-1HP-10, Mabtech AB, Sweden), containing mAb MT126L ...