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Citations for Mabtech :
151 - 191 of 191 citations for IFN gamma Mouse since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... pulsed with WT1328-348 (10 µg/ml) followed by measurement of specific cytokine production using the human IFN-γ ELISpot kit (Mabtech AB) and ELIPHOTO Counter (Minerva Tech).
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bioRxiv - Cancer Biology 2021Quote: ... Plates were developed by washing 6 times in PBS/0.05%Tween 20 followed by incubation with biotinylated IFN-γ detection antibody (0.5 mg/mL; Mabtech; 2 hours, 37°C). This was followed by a further 6 washes in PBS/0.05%Tween 20 and incubation with avidin DH-coupled biotinylated peroxidase H complex (Vectastain Elite ABC Kit ...
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bioRxiv - Immunology 2022Quote: ... were pre-wet with 30% ethanol for 60 seconds and then coated with 100 µl primary anti-IFN-γ antibody solution (10 µg/ml of 1-D1K coating antibody from Mabtech, Cincinnati, OH) OVN at 4°C ...
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bioRxiv - Immunology 2022Quote: ... This cell and peptide mixture was loaded onto a 96-well PVDF plate coated with anti-IFN-γ (3420-2HW-Plus, Mabtech, Nacka, Sweden) and cultured overnight ...
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bioRxiv - Immunology 2020Quote: ... Cellular immune responses were measured by IFN-γ FluoroSpot assay according to the manufacturer’s instructions (Cat. No. FSP-4246-2 Mabtech, Inc., Cincinnati, OH). Briefly ...
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bioRxiv - Immunology 2024Quote: ... were pre-wet with 30% ethanol for 60 seconds and then coated with 100 µl primary anti-IFN-γ antibody solution (10 µg/ml of 1-D1K coating antibody from Mabtech, Cincinnati, OH) OVN at 4°C ...
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bioRxiv - Immunology 2019Quote: ... Approximately 100,000 cells in 100 μl of R10 media were layered on precoated monkey IFN-γ ELISpot-Plus plates (Mabtech Inc., Mariemont, Ohio, USA) with a single peptide per well at a final concentration of 1 μM ...
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bioRxiv - Immunology 2022Quote: ... kits with pre-coated plates for enumeration of cells secreting IFN-γ and IL-17A were purchased from Mabtech (Cat. No. FSP-414443-2). Spots were enumerated on an AID iSpot EliSpot/ FluoroSpot Reader.
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bioRxiv - Immunology 2020Quote: ... at 37 °C and 5% CO2 with overlapping peptide pools representative of either S1 or S2 SARS-CoV-2 proteins (JPT, #PM-WCPV-S) at 1 μg/ml each or DMSO in 96-well ELISpot IFN-γ plates (Mabtech #3321-4APW-2) in triplicates ...
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bioRxiv - Immunology 2021Quote: ... washed with sterile PBS and coated overnight at 4 °C with anti–IFN-γ antibody (10 µg/ml in PBS; clone 1-D1K; Mabtech, Nacka Strand, Sweden). Prior to use ...
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bioRxiv - Immunology 2023Quote: ... Wells were washed and incubated for 2 hours with biotinylated anti-IFN-ψ detection antibody (mAb-7-B6-1-Biotin, Mabtech, Cat. 3420-6-250). Afterwards ...
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bioRxiv - Immunology 2020Quote: ... were pre-wet with 70% methanol and then coated with 100 µl primary anti-IFN-γ antibody solution (10 µg/ml of 1-D1K coating antibody from Mabtech in PBS, pH 7.4, V-E4) OVN at 4°C ...
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bioRxiv - Immunology 2021Quote: ... (MabTech, Mouse IFNγ ELISpot Plus). Cells were washed off ...
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bioRxiv - Bioengineering 2024Quote: ... Anti-mouse IgG capture antibody (Mabtech) diluted to 15 µg/mL in PBS was then added to each well before incubation overnight at 4°C ...
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bioRxiv - Immunology 2021Quote: Mouse IL-2 (MABTECH, 3441-4APW-2), IL-4 (MABTECH ...
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bioRxiv - Microbiology 2022Quote: IL-4 and IL-2 ELISPOT assays were performed with mouse IL-4 ELISPOTPLUS kits and mouse IL-2 ELISPOTPLUS kits according to the manufacturer’s instructions (Mabtech). IFN-γ ELISPOT assays were performed with mouse IFN-γ Precoated ELISPOT kits according to the manufacturer’s instructions (Dakewe Biotech) ...
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bioRxiv - Immunology 2022Quote: IFNγ and IL4 ELISpot assay was performed using Mouse IFNγ ELISPOT basic kit (ALP) and Mouse IL4 ELISPOT basic kit following the manufacturer’s instructions (Mabtech, VA, USA). For cell activation control ...
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bioRxiv - Microbiology 2019Quote: ... an enzyme-linked immunosorbent assay kit detecting mouse IgG (Mabtech) was used ...
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bioRxiv - Immunology 2021Quote: ... followed by incubation with biotin-conjugated anti-mouse IgG (Mabtech In) for 2 h at room temperature ...
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bioRxiv - Immunology 2021Quote: ... goat anti-mouse IgA biotin (Mabtech, Cat no. 3865-6-250) at (1:500 ...
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bioRxiv - Cancer Biology 2022Quote: ... IFNΨ secretion was assessed biotinylated anti-mouse IFNΨ (R4-6A2; Mabtech) and imaged using an ELISPOT reader (AID Diagnostika) ...
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bioRxiv - Microbiology 2023Quote: ... followed by incubation with biotin-conjugated anti-mouse IgA (Mabtech In) for 2 h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Mouse IL-17A ELISpotPLUS kit ALP (MABTECH, Cat# 3521-4APW-2) was performed following manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... ELISpotPLUS plates as well as Mouse IFNγ ELISpotPRO plates (MABTECH, 3421M-2AST-2) were washed with PBS and blocked overnight at 4°C in RPMI 1640 media (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ELISpot assays were performed with mouse IFNγ ELISpotPLUS kits according to the manufacturer’s instructions (Mabtech). A total of 5 × 105 splenocytes was ex vivo restimulated with the full-length S peptide mix (0.1 µg/mL final concentration per peptide ...
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bioRxiv - Immunology 2022Quote: ... IFNγ ELISPOT was performed using the Mouse IFNγ ELISPOTPLUS kit (MABTECH product # 3321-4HPW-10). ELISPOT plates were analyzed using ImmunoSpot CTL plate reader (CTL ImmunoSpot S6 Entry Analyzer ...
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bioRxiv - Immunology 2020Quote: ... Antigen-specific IgA titers in lungs were detected using a mouse IgA ELISA kit (Mabtech) and pNPP substrate (Mabtech) ...
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bioRxiv - Microbiology 2023Quote: ... the wells were coated with 100 µl of anti-mouse IgA capture Ab (Mabtech In). Cells were added in duplicates to assess total IgA ASCs or SARS-CoV-2 specific B cells ...
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bioRxiv - Immunology 2020Quote: Murine ELISpot assays were performed with mouse IFNγ ELISpotPLUS kits according to the manufacturer’s instructions (Mabtech). A total of 5 × 105 splenocytes was ex vivo restimulated with the full-length S peptide mix (0.1 μg/mL final concentration per peptide ...
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bioRxiv - Immunology 2021Quote: ... the wells were coated with 100 μL of anti-mouse IgG or IgA capture Ab (Mabtech In). Stimulated cells were harvested ...
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bioRxiv - Microbiology 2022Quote: ... or IL-5 were determined using a mouse enzyme-linked immunospot (ELISpot) assay kit (Mabtech, Cincinnati, OH) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... then washed with PBS-Tween 0.01% before to add anti-mouse IgG antibody (Mabtech – 1/500 dilution). After the last washing ...
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bioRxiv - Immunology 2023Quote: ... and incubated with 1 μg/ml of the biotinylated anti-mouse IFNγ monoclonal antibody R4-6A2 (Mabtech) diluted in PBS-0.5% FCS for 2 h ...
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bioRxiv - Immunology 2023Quote: ... 96-well nitrocellulose-bottom plates pre-coated with anti-mouse IFNγ monoclonal antibody were purchased from Mabtech. The plates were blocked with RPMI-10% FBS for at least 30 minutes ...
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bioRxiv - Immunology 2024Quote: ... then incubated at room temperature with biotinylated secondary goat anti-mouse IgG (catalogue #3825-6-250, Mabtech) at a 2 µg/mL concentration in the dark for 2 hours ...
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bioRxiv - Immunology 2023Quote: The number of B cells secreting IgG was quantified using a mouse ELISpot kit (Mabtech, 3825-2H). The isolated cells were counted using the Countess Automated Cell Counter II (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed with PBS and then incubated with anti-monkey or anti-mouse IFNγ mAb biotin (Mabtech) for two hours ...
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bioRxiv - Immunology 2022Quote: ... 100 µL of either goat anti-mouse IgG (for 6E2 hybridoma cells) or mouse anti-human IgG (for EBV-immortalized human B cells) specific for the Fc region (Mabtech) diluted in sterile PBS at 15 µg/mL were added to wells followed by incubation at 4°C for 24 hours ...
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bioRxiv - Immunology 2022Quote: ... ELISpot assays for IFNγ were performed as instructed by the manufacturer using the Mouse IFNγ ELISpot Set (MabTech, cat# Q00824-1×20210623DS). 0.1×106 mouse splenocytes were plated into each well and stimulated overnight with 0.2 µg per peptide per well of RBD-derived overlapping peptides (GenScript).
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bioRxiv - Immunology 2022Quote: Single cell suspensions from lungs and spleens of mice were stimulated with mouse IL-2 (R & D Systems, Cat # 402-ML; 0.5 μg/mL) and RD848 (Mabtech, Cat # 3611-5X; 1 μg/mL) for 3 days to induce nonspecific polyclonal expansion ...
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bioRxiv - Immunology 2022Quote: ... the cells were washed and plated onto PVDF ELISpot filter plates coated with anti-mouse IgG or IgA capture antibody (Mabtech, Cat# BASIC 3825-2H and BASIC 3835-2H). The plates were incubated at 37°C for 24 hours ...