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Citations for Mabtech :
101 - 135 of 135 citations for Indusatumab Anti GUCY2C since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... we washed off the cells and developed the plates using biotinylated R4-6A2 anti-IFN-γ (Mabtech #3321-6-250) and BVD6-24G2 anti-IL4 (Mabtech #3311-6-250 ...
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bioRxiv - Microbiology 2022Quote: ... Elispot plates were pre-coated with anti-IFNγ primary antibody and after cell-incubation IFNγ revealed using anti-IFNγ secondary antibody conjugated with biotin (both from Mabtech) as described (Cardinaud et al. ...
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bioRxiv - Immunology 2023Quote: ... and the plate was incubated for 2 h at room temperature with R4-6A2 biotinylated anti-IFN-γ antibody (Mabtech) at the concentration of 100 μg/mL ...
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Metabolomics reveals nucleoside analogs for regulating mucosal-associated invariant T cell responsesbioRxiv - Immunology 2023Quote: ... The IFNγ+ MAIT cell spots were then developed with an indirect immunostain approach sequentially using a biotinylated anti-human IFNγ antibody (Mabtech), ExtraAvidin conjugated by alkaline phosphatase (Sigma) ...
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bioRxiv - Immunology 2024Quote: ... which were previously (1-7 days) coated with 4 μg/mL of anti-IFN-γ antibody (clone 1-D1k, Mabtech) and blocked by incubating at least for 1 hour with RPMI medium containing 10% human serum ...
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bioRxiv - Microbiology 2024Quote: ... followed with adding 50 µl per well of 1 µg/ml biotin rat anti-mouse IFNγ (Mabtech, 3321-6-1000) into each well and incubated for 2 hours at room temperature ...
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bioRxiv - Immunology 2024Quote: The amounts of IgA and sIgA were measured by ELISA protocols including capture mAbs anti-human IgA (clone MT57, Mabtech) for IgA and anti-human secretory component (clone SC-05 ...
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bioRxiv - Immunology 2024Quote: ... ELISPOT plates were pre-coated with anti-IFNγ primary antibody and after cell-incubation IFNγ revealed using anti-IFNγ secondary antibody conjugated with biotin (both from Mabtech) as described 24 ...
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bioRxiv - Immunology 2024Quote: ... Plates were first incubated with 0.2 mg/mL of biotinylated anti-IFN-γ antibody (clone 7-B6-1 by Mabtech) for 2 hours at 37°C ...
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bioRxiv - Immunology 2022Quote: ... the ELISPOT plates were washed and stained with anti-IFN-γ-biotin followed by streptavidin-conjugated HRP (3420-2HW-Plus, Mabtech). Plates were developed using TMB substrate and read using a CTL-ImmunoSpot® S6 Analyzer (Cellular Technology Limited ...
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bioRxiv - Immunology 2020Quote: ... and incubated for 2 hr at room temperature with biotinylated anti-IFN-γ (clone mAb-7B6-1; 1 μg/ml Mabtech). After six further washes ...
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Dendritic cells focus CTL responses toward highly conserved and topologically important HIV epitopesbioRxiv - Immunology 2020Quote: ... T cells were resuspended at a concentration of 3×105 cells per milliliter and 100 μl (3×104 cells/well) were transferred to IFNγ-antibody coated 96-well ELISpot plates (5μg/ml anti-IFNγ mAb 1-D1K, Mabtech, Stockholm, Sweden). Individual 9mer peptide dilutions were done in a separate 1 ml deep-well 96-well plate at a final concentration of 2μg/ml and 100 μl of this dilution was added to T cell-containing wells to give a final peptide concentration of 1μg/ml ...
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bioRxiv - Immunology 2020Quote: ... IFN-γ spot forming cells (SFC) were detected by staining membranes with anti-mouse IFN-γ biotin mAb (1 µg/mL; clone R46A2, Mabtech) followed by streptavidin-alkaline phosphatase (1 µg/mL ...
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bioRxiv - Immunology 2021Quote: ... followed by two washes with PBS and then incubated for 2 hr at RT with biotinylated anti-IFN-γ (1 μg/mL; clone mAb-7B6-1; Mabtech). After six further washes ...
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bioRxiv - Immunology 2022Quote: ... followed by 2 hours of incubation at 37°C with biotinylated anti-human IFN-γ monoclonal antibodies (clone 7-B6-1, Mabtech) diluted at 1μg/ml in PBS ...
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bioRxiv - Microbiology 2023Quote: ... The plates were incubated at 37 °C in 5 % CO2 for 18 h and washed with PBS before the addition of 1 µg/ml biotinylated anti-IFN-γ mAb (Mabtech) at room temperature for 2 h ...
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bioRxiv - Immunology 2024Quote: ... The plates were washed with DPBS-Tween 7 times and then incubated with 1µg/mL per well of biotinylated anti-human IFN-γ (MabTech, USA) for 2 to 4 hours at room temperature ...
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bioRxiv - Immunology 2022Quote: Incubate the array with 10 μg/mL biotinylated anti-human IFNγ 7-B6-1 (MABTECH AB, Stockholm, Sweden, Cat #3420-6-250) and then 12 μg/mL AF647-Streptavidin (Jackson ImmunoResearch ...
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bioRxiv - Immunology 2020Quote: ... 5% CO2 at a concentration of 1×105 cells/mL media on plates previously coated with anti-human IFNγ (Mab 1-D1K; Mabtech, Stockholm, Sweden). Cells were then discarded and plates were further incubated with biotinylated IFNγ antibody (Mab 7-B6-1 ...
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bioRxiv - Immunology 2020Quote: ... Controls included 1×105 effector CD8+ T cells co-cultured with anti-human CD3 monoclonal antibody (0.5 µg/mL, Mabtech, 3605-1-1000), or with media alone ...
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bioRxiv - Immunology 2020Quote: ... for the enumeration of total IgG-secreting cells with 1 µg per well of anti-porcine IgG (mAb, MT421, Mabtech AB, Sweden) or with culture medium supplemented with 10% FBS (background) ...
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bioRxiv - Immunology 2021Quote: 96-well FluoroSpot plates were coated with anti-cytokine antibodies for IFNγ and IL-5 (mAbs 1-D1K and TRFK5, respectively; Mabtech, Stockholm, Sweden) at a concentration of 10μg/mL ...
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bioRxiv - Immunology 2020Quote: ... and for the enumeration of total IgG-secreting cells with 1 µg per well of anti-porcine IgG (mAb, MT421, Mabtech AB, Sweden) or with culture medium supplemented with 10% FBS (media background control) ...
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bioRxiv - Immunology 2024Quote: ... cells were discarded and detection of IFN-γ was performed by adding the anti-human IFN-γ biotinylated mAb (clone 7-B6-1, MabTech) for 2h ...
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bioRxiv - Immunology 2024Quote: ... plates were incubated for 1h at RT with a biotin conjugated anti-IL-12p70 detection antibody (1 μg/ml; Mabtech clone MT704) diluted to 1 μg/ml before the process was repeated using streptavidin conjugated to alkaline phosphatase (Mabtech ...
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bioRxiv - Immunology 2022Quote: ... were pre-wet with 30% ethanol for 60 seconds and then coated with 100 µl primary anti-IFN-γ antibody solution (10 µg/ml of 1-D1K coating antibody from Mabtech, Cincinnati, OH) OVN at 4°C ...
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bioRxiv - Immunology 2022Quote: ... This cell and peptide mixture was loaded onto a 96-well PVDF plate coated with anti-IFN-γ (3420-2HW-Plus, Mabtech, Nacka, Sweden) and cultured overnight ...
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bioRxiv - Immunology 2024Quote: ... were pre-wet with 30% ethanol for 60 seconds and then coated with 100 µl primary anti-IFN-γ antibody solution (10 µg/ml of 1-D1K coating antibody from Mabtech, Cincinnati, OH) OVN at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... 0.5×106 lung cells or 0.5×105 BALF cells in 100 µl RPMI complete media were added into each well of purified rat anti-mouse IFNγ (Mabtech, 3321-3-1000) pre-coated ELISpot plates ...
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bioRxiv - Immunology 2021Quote: ... washed with sterile PBS and coated overnight at 4 °C with anti–IFN-γ antibody (10 µg/ml in PBS; clone 1-D1K; Mabtech, Nacka Strand, Sweden). Prior to use ...
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bioRxiv - Immunology 2024Quote: ... the plates were coated with a capture antibody cocktail containing 10 µg/mL mouse anti-human of each IFN-γ (Mabtech, clone: 1-D1K), IL-5 (Mabtech ...
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bioRxiv - Immunology 2024Quote: ... Wells were washed and incubated for 2 hours with biotinylated anti-IFN-γ detection antibody (mAb-7-B6-1-Biotin, Mabtech, Cat. 3420-6-250). Afterwards ...
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bioRxiv - Immunology 2020Quote: ... then wells were washed 5 times with sterile D-PBS and incubated with anti–IFNγ biotinylated antibody (1 µg/mL per well, Mabtech, mAb 7-B6-1, 3420-9H) for 2 hours.
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bioRxiv - Immunology 2020Quote: ... were pre-wet with 70% methanol and then coated with 100 µl primary anti-IFN-γ antibody solution (10 µg/ml of 1-D1K coating antibody from Mabtech in PBS, pH 7.4, V-E4) OVN at 4°C ...
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bioRxiv - Immunology 2022Quote: ... the cells were washed and plated onto PVDF ELISpot filter plates coated with anti-mouse IgG or IgA capture antibody (Mabtech, Cat# BASIC 3825-2H and BASIC 3835-2H). The plates were incubated at 37°C for 24 hours ...