Labshake search
Citations for Mabtech :
101 - 126 of 126 citations for IL 6 Mouse since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: IFN-γ ELISPOT assay was performed using a Mouse IFN-γ ELISpot antibody pair (Mabtech, Sweden). Splenocytes were isolated in RPMI culture medium (Gibco ...
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bioRxiv - Immunology 2022Quote: ... Spot-forming units (SFU) were quantified using a mouse IFN-γ ELISpot kit (Mabtech, Stockholm, Sweden) per the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... the wells were coated with 100 μL of anti-mouse IgG or IgA capture Ab (Mabtech In). Stimulated cells were harvested ...
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bioRxiv - Immunology 2020Quote: ... then washed with PBS-Tween 0.01% before to add anti-mouse IgG antibody (Mabtech – 1/500 dilution). After the last washing ...
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bioRxiv - Immunology 2023Quote: ... 96-well nitrocellulose-bottom plates pre-coated with anti-mouse IFNγ monoclonal antibody were purchased from Mabtech. The plates were blocked with RPMI-10% FBS for at least 30 minutes ...
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bioRxiv - Immunology 2023Quote: ... and incubated with 1 μg/ml of the biotinylated anti-mouse IFNγ monoclonal antibody R4-6A2 (Mabtech) diluted in PBS-0.5% FCS for 2 h ...
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bioRxiv - Immunology 2023Quote: The number of B cells secreting IgG was quantified using a mouse ELISpot kit (Mabtech, 3825-2H). The isolated cells were counted using the Countess Automated Cell Counter II (ThermoFisher ...
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bioRxiv - Immunology 2021Quote: ... and 2 x 105 splenocytes were plated into each well of the Mouse IFN-γ ELISpotPLUS plates (MabTech). The splenocytes were stimulated for 20 hours at 37°C with RBD peptides (15-mer peptides overlapping by 9 amino acid spanning the RBD of SARS-CoV-2 spike protein ...
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bioRxiv - Immunology 2021Quote: ... Plates were washed with PBS and then incubated with anti-monkey or anti-mouse IFNγ mAb biotin (Mabtech) for two hours ...
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bioRxiv - Immunology 2020Quote: Assessment of the IFN-γ T cell response was performed using the Mouse IFN-γ ELISpotPLUS kit (Mabtech) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... and incubated with 50 μL of biotinylated anti-mouse IFN-γ R4-6A2 mAb (1:1000 dilution; Mabtech) diluted in PBS with 1% FBS for 1 hour at RT ...
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bioRxiv - Immunology 2021Quote: ... FluoroSpot assay was performed using mouse IFN-γ FluoroSpotPLUSkit according to the manufacturer’s instructions (Mabtech, Inc., Cincinnati, OH). Briefly ...
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bioRxiv - Bioengineering 2023Quote: Assessment of the IFN-γ T cell response was performed using the Mouse IFN-γ ELISpotPLUS kit (Mabtech) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Splenocytes were counted and 100,000 cells/well added to the plates of the Mouse IFN-g ELISpotPLUS (HRP) kit (MABTECH). Cells were then stimulated for 18 hours at 37 °C with SARS-CoV-2 S PepTivator peptides (Miltenyi Biotec ...
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bioRxiv - Microbiology 2022Quote: ... Miltenyi Biotech) for 24 h in ELISpot plates coated with anti-mouse-IFN antibody (Mabtech AB, Nacka Strand, Sweden). The procedures were carried out according to the manufacturer’s instructions (Mabtech ...
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bioRxiv - Immunology 2021Quote: ... Plates were then washed and incubated with biotinylated anti-mouse IFN-γ antibody (clone R4-6A2; Mabtech; 1 μg/mL) followed by streptavidin-alkaline phosphatase (Mabtech ...
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bioRxiv - Immunology 2022Quote: ... 100 µL of either goat anti-mouse IgG (for 6E2 hybridoma cells) or mouse anti-human IgG (for EBV-immortalized human B cells) specific for the Fc region (Mabtech) diluted in sterile PBS at 15 µg/mL were added to wells followed by incubation at 4°C for 24 hours ...
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bioRxiv - Microbiology 2020Quote: ... The stimulated splenocytes were analyzed for IFN-γ responses using the mouse IFN-γ ELISpot Plus kit (Mabtech AB, Sweden). Spot-forming cells (SFU ...
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bioRxiv - Immunology 2020Quote: IFN-γ ELISpot was performed on splenocytes of mice isolated after sacrifice using mouse IFN-γ ELISpot-plus kit (Mabtech). Splenocytes were obtained by disaggregation of spleens with the gentleMACS dissociator ...
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Efficacy of recombinant NP-M1 and NP-M1-CRT DNA vaccines against Influenza A viruses in mice C57/BL6bioRxiv - Molecular Biology 2021Quote: ... The number of NP-specific IFN□ secreting cells in mouse was counted using commercial ELISPOT assay kits (MABTECH AB, Sweden). This procedure was conducted according to the instruction manual ...
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bioRxiv - Immunology 2020Quote: ... IFN-γ spot forming cells (SFC) were detected by staining membranes with anti-mouse IFN-γ biotin mAb (1 µg/mL; clone R46A2, Mabtech) followed by streptavidin-alkaline phosphatase (1 µg/mL ...
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bioRxiv - Immunology 2022Quote: ... ELISpot assays for IFNγ were performed as instructed by the manufacturer using the Mouse IFNγ ELISpot Set (MabTech, cat# Q00824-1×20210623DS). 0.1×106 mouse splenocytes were plated into each well and stimulated overnight with 0.2 µg per peptide per well of RBD-derived overlapping peptides (GenScript).
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bioRxiv - Immunology 2022Quote: Spleens from immunized animals were removed at Day 51 for the mice and at Day 110 for the hamsters and splenocytes were isolated for protein S-specific T-cell detection with the ELISpotPLUS mouse IFN-γ or ELISpotPLUS hamster IFN-γ kits (Mabtech) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Interferon (IFN)-γ Enzyme-linked ImmunoSpot (ELISPOT) assay was performed as described previously160 using the Mouse IFN-γ ELISpot kit (Mabtech, Stockholm, Sweden) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... and ELISPOT plates were developed following the manufacturer’s protocol for the ELISpot Plus: Mouse IFN-γ (ALP) kit (Mabtech Cat. No. 3321-4APW). After the plates were developed ...
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bioRxiv - Immunology 2022Quote: ... the cells were washed and plated onto PVDF ELISpot filter plates coated with anti-mouse IgG or IgA capture antibody (Mabtech, Cat# BASIC 3825-2H and BASIC 3835-2H). The plates were incubated at 37°C for 24 hours ...